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Serotype and Leukotoxic Strain Distribution of Actinobacillus(Haemophilus) Actinomycetemcomitans in Korean Localized Juvenile Periodontitis (한국인 국소 유년성 치주염환자의 Actinobacillus(Haemophilus) Actinomycetemcomitans 혈청형 및 백혈구독성 균주 분포)

  • Chung, Hyun-Ju;Chung, Chong-Pyoung;Son, Seong-Heui
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.4
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    • pp.487-501
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    • 1986
  • Previous studies from our laboratory suggested that Korean LJP patients might habor A. actinomycetemcomitans of different serotype from Caucasian LJP patients in whom serotype b was predominant. In order to observe the prevalence and serotype distribution of A. actinomycetemcomitans in localized juvenile periodontitis patients and to evaluate leukotoxic activity of oral isolates, this study was performed. A. actinomycetemcomitans was isolated by using a selective medium(tryptic soy agar supplemented with 10% serum, $75{\mu}g$ of bacitracin and $5{\mu}g$ of vancomycin per ml). Using immunoabsorbed, ammonium sulfate-fractionated serotype-specific antisera, a total of 69 strains were serologically categorized by ELISA. Leukotoxicity was monitored biochemically by measuring lactate dehydrogenase indicator of cell viability in culture supernatant of PMNL plus viable A. actinomycetemcomitans mixture. The results were as follows: 1. A. actinomycetemcomitans was detected in 75% of 16 LJP patients, and 71% in the LJP lesions and 6% in the control sites. 2. Presence or absence of A. actinomycetemcomitans in the sampled disease sites has no in fluence on clinical measurements. 3. Three serotypes were approximately equally distributed in overall 9 patients. Three patients harbored 2 different serotypes of A. actinomycetemcomitans in the same disease site or different disease sites. 4. The proportion of leukotoxic oral isolates was 22% of a total of 46 strains and the prevalence was 69% in 13 sampled sites. The same disease site could harbor both leukotoxic and nonleukotoxic strains. 5. Distribution of leukotoxic strains in 3 serotypes were not different.

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Studies on Hilly Pasture Landscape Expectancy, Satisfaction of Tourist on Grassland Facility: A case Study of Yangtae Farm Visitor (산지목장 방문자의 목장 경관 기대와 목초지 및 초지시설 만족도에 관한 연구 : 양떼목장 방문객의 경우)

  • Kang, Dae-Koo;Lee, Hyo-Jin;Lee, Hyowon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.1
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    • pp.68-79
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    • 2017
  • The objective of study was to find the relationship in hilly pasture landscape expectancy and tourist's satisfaction on grassland facility. It was followed by literature reviews and visitors' survey in Daegwallyeong Yangtte Farm on 31, July, 2014. 367 respondents were analyzed by F-test, t-test, Chi-square and Fisher's Exact Test at 0.05 level after data screening process. Computing factors were sex, marital status, age, academic career and occupation. The results was as followed; First, major respondents group of survey were in oder of woman, forty years old group, married office worker, and university graduate. Second, the expectancy for grassland was significant difference in age, but pasture color expectancy was not significant difference in gender, age, educational background, marital status, and there was significant difference in favorite grassland type with age, marital status. Third, favorite fence type was not significant difference along with all group of participants. However, color and material of fence was significant difference in marital status. Fourth, preferred ranch road was significant difference with occupation and marital status. There was significant difference in favorite grassland type near ranch road along with age and occupation type. Fifth, the mean satisfaction was 3.6 point in 5.0. Therefore, all respondents were generally satisfaction in visited. Tourists were more interested in ranch landscape than experience or contacts to animal.

Effect of Vegetation Types on the Distribution of Soil Invertebrates (식생유형이 토양무척추동물 분포에 미치는 영향)

  • Kim, Myung-Hyun;Bang, Hea-Son;Han, Min-Su;Hong, Hey-Kyoung;Na, Young-Eun;Kang, Kee-Kyung;Lee, Jeong-Taek;Lee, Deog-Bae
    • Korean Journal of Environmental Agriculture
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    • v.28 no.2
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    • pp.125-130
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    • 2009
  • The aim of this study was to investigate whether differences in the distribution of soil invertebrates among different vegetation types (forest, reservoir, and crop land types) in rural area. A total of 18 orders and 137 species were collected by pitfall traps. Species numbers were the lowest (33 species) at the Chamaecyparis obtusa plantation (St. 6). On the forest sites, the individual number of Hymenoptera was the most abundant, and Acari and Coleoptera was the relatively more abundant than the other sites. On the reservoir sites (Salix chaenomeloides community), the individual number of Collembola was the most abundant, and Diptera was the relatively more abundant than the other sites. On the crop land sites, the individual numbers of Collembola, Hymenoptera, and Araneae were the relatively more abundant than the other orders. The density of Araneae was higher in the reservoir and crop land sites than in the forest sites. From a point of view of biodiversity, although the diversity index(H') was the highest in the mixed broad-leaved forest type (St. 2) with Quercus serrata and Q. acutissima, and the lowest in the upland levee of crop land(St. 11), there was no significant difference among the habitat or vegetation types. According to the community analysis, the soil invertebrates could be divided into 4 groups, the mixed broad-leaved forest type (A group), the plantation or pure forest type (B group), the reservoir type (C group), and the crop land type (D group).

Effects of Sowing Method and Summer Management on Yield , Dead Matter , Weed Development and Ground Cover of Orchargrass (Dactylis glomerata L.) Meadow (파종방법 및 여름철관리가 Orchargrass ( Dactylis glomerata L. ) 채초지의 수량 , 고사물량 , 잡초발생 및 피복율에 미치는 영향)

  • 권찬호;김동암
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.7 no.2
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    • pp.71-78
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    • 1987
  • This experiment was carried out to examine the effects of sowing method and summer management on the dry matter yield, dead matter, weed development and ground cover of orchardgrass (Dactylis glomerata L.) meadow. The experiment was allocated as a split-split plot design with three replications. The main plots were sowing method of drilling and broadcasting, sub plots were drainage of experimental field, adequate and inadequate, and sub-sub plots were cutting timeof orchardgrass, cutting before rainy season started and cutting after rainy season ended. The experiment was undertaken over a period of 14 months from September, 1983 to October, 1984. The results obtained are summarized as follows: 1. There were no significant differences in dry matter yield among treatments at the first cutting, but cutting before rainy season produced significantly more forage yield ($P{\le}0.01$) than cutting after rainy season at the second and third cuts. At the third cutting, drilled orchardgrass meadow showed a significant dry matter yield ($P{\le}0.05$) than broadcast orchardgrass meadow, 2. The dead matter of orchardgrass was accumulated only at the second cutting when orchardgrass meadow cut after rainy season. Orchardgrass produced in the adequate and inadequate drainage plots consisted 20.4 and 35.9% of dead material, respectively, but no significant difference was found between two drainage treatments. 3. Drilled orchardgrass meadow produced significantly less weeds ($P{\le}0.05$) than broadcast orchardgrass meadow, but the plots cut after rainy season produced significantly more weeds ($P{\le}0.01$) than the plots cut before rainy season. 4. The percent ground cover of orchardgrass in the plots cut before rainy season was significantly higher ($P{\le}0.01$) than that in the plots cut after rainy season at the second cutting. Drilled plots showed a slight increase in the ground cover than the broadcast, but the difference was not significant. The same trend of ground cover of the meadow estimated at the second cutting was sustained after the third cutting. 5. Based on the results of the experiment, it indicates that the second cut of orchardgrass should be made before rainy season related for maintaining high yield of the meadow. Drilling as a sowing method of orchardgrass meadow could be adopted in the view point of reducing weed development.

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Diagnosis of Pigs Producing PSE Meat using DNA Analysis (DNA검사기법을 이용한 PSE 돈육 생산 돼지 진단)

  • Chung Eui-Ryong;Chung Ku-Young
    • Food Science of Animal Resources
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    • v.24 no.4
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    • pp.349-354
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    • 2004
  • Stress-susceptible pigs have been known as the porcine stress syndrome (PSS), swine PSS, also known as malignant hyperthermia (MH), is characterized as sudden death and production of poor meat quality such as PSE (pale, soft and exudative) meat after slaughtering. PSS and PSE meat cause major economic losses in the pig industry. A point mutation in the gene coding for the ryanodine receptor (RYR1) in porcine skeletal muscle, also known calcium (Ca$^{2+}$) release channel, has been associated with swine PSS and halothane sensitivity. We used the PCR-RFLP(restriction fragment length polymorphism) and PCR-SSCP (single strand conformation polymorphism) methods to detect the PSS gene mutation (C1843T) in the RYR1 gene and to estimate genotype frequencies of PSS gene in Korean pig breed populations. In PCR-RFLP and SSCP analyses, three genotypes of homozygous normal (N/M), heterozygous carrier (N/n) and homozygous recessive mutant (n/n) were detected using agarose or polyacrylamide gel electrophoresis, respectively. The proportions of normal, carrier and PSS pigs were 57.1, 35.7 and 7.1% for Landrace, 82.5, 15.8 and 1.7% far L. Yorkshire, 95.2, 4.8 and 0.0% for Duroc and 72.0, 22.7 and 5.3% for Crossbreed. Consequently, DNA-based diagnosis for the identification of stress-susceptible pigs of PSS and pigs producing PSE meat is a powerful technique. Especially, PCR-SSCP method may be useful as a rapid, sensitive and inexpensive test for the large-scale screening of PSS genotypes and pigs with PSE meat in the pork industry.y.

A Study on the Irrigation Water Pollution of the Gimhae Plain (김해평야(金海平野)의 관개수(灌漑水) 오염(汚染)에 관한 연구(硏究))

  • Ha, Ho-Sung;Heo, Jong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.1 no.1
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    • pp.22-30
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    • 1982
  • Water quality of downstream of the Nagdong river, using for agricultural irrigation of the Gimhae plain, were observed. Water temperature, turbidity, residue, pH, BOD, COD, DO, hardness, chloride, sulphate, phosphate, inorganic nitrogenous compounds, sodium, general bacteria, E. coli and heavy metals of the water were investigated at Daejeo, Sikman, Bongrim, Noksan, Machal and Jangyou pumping stations in the Gimhae plain in May, July and October, 1981. The results obtained were as follows; 1) Average value of analyzed components of the water at all sampling sites were 7.8 pH, 6.3 ppm BOD, 6.5 ppm COD, 6.4 ppm DO, 231 ppm hardness, 582 ppm Cl-, 412 ppm $SO_4--$, 2.32 ppm $PO_4---$, 3.8 ppm $NH_4+,\;478\;ppm\;Na+$, 2964 No. /100 ml total coliform, 0.0040 ppm Cd, 0.0066 ppm Pb, respectively. 2) The most heavily polluted site of all investigated ones was Sikman. It seemed to be caused by the vast quantity of wastewater discharged from industrial district in Gimhae city. The next polluted sites were Bongrim, Daejeo and Noksan, and comparatively less polluted sites were Machal and Jangyou, judging from both appearance and physicochemical observation. 3) At Sikman, the most heavily polluted site, average value of components were 8.0 pH, 8.1 ppm BOD, 8.2 ppm COD. These values were close to the limit point of agricultural water quality standard of 8.0 ppm BOD (COD). 4) Any apparent variation was not observed by the sampling season in most components except DO and $NH_4+$. DO of October was higher than that of May or July but $NH_4+$ was low. 5) $NH_4+$ content was comparatively high in downstream of the Nagdong river of which water is used as the agricultural irrigation in the Gimhae plain. Therefore, fertilizer application on the farming land must make account of nitrogen content of the irrigation water 6) It was considered that chloride and sodium contents would not influence the crop cultivation in common season, but in dry season irrigation must be done carefully.

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The implication of capital restructuring on urban development : Chicago politics as the local contingent facter for urban restructuring (자본재구조화가 도시발달에 미치 는 영향:시카고 정치와 재개발사업을 사례로)

  • Koh, Tae Kyung
    • Journal of the Korean Geographical Society
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    • v.29 no.4
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    • pp.420-437
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    • 1994
  • The starting point of the research is the relation between capital restructuring and urban restructuring. The economic restructuring, which has been caused by the economic crisis in the early 1970s in the United States has brought a spatial restructuring at different geographic scales. The degree of the success of urban restructuring is contingent to the local economic and political environments. The local contingent factor such as local politics should not be neglected for investigating the restructuring process. Through the case study of Chicago, the research provides two inconsistencies in applying the structural approach to the local level: first, the lack of the theoretical link between crisis and restructuring; and second, the crucial importance of local politics in shaping urban development.

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Effect of prosthetic designs and alveolar bone conditions on stress distribution in fixed partial dentures with pier abutments (중간 지대치가 존재하는 고정성 국소의치에서 보철물 설계 및 치조골 상태가 응력분포에 미치는 영향)

  • Cho, Wook;Kim, Chang-Seop;Jeon, Young-Chan;Jeong, Chang-Mo
    • The Journal of Korean Academy of Prosthodontics
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    • v.47 no.3
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    • pp.328-334
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    • 2009
  • Statement of problem: Pier abutments act as a Class I fulcrum lever system when the teeth are incorporated in a fixed partial denture with rigid connectors. Therefore non-rigid connector incorporated into the fixed partial denture might reduce the stresses created by the leverage. Purpose: The purpose of this study was to evaluate, by means of finite element method, the effects of non-rigid connectors and supporting alveolar bone level on stress distribution for fixed partial dentures with pier abutments. Material and methods: A 2-dimensional finite element model simulating a 5-unit metal ceramic fixed partial denture with a pier abutment with rigid or non-rigid designs, the connector was located at the distal region of the second premolar, was developed. In the model, the lower canine, second premolar, and second molar served as abutments. Four types of alveolar bone condition were employed. One was normal bone condition and others were supporting bone reduced 20% height at one abutment. Two different loading conditions, each 150 N on 1st premolar and 1st molar and 300N on 1st molar, were used. Results: Two types of FPD were displaced apically. The amount of displacement decreased in an almost linear slope away from the loaded point. Non-rigid design tended to cause the higher stresses in supporting bone of premolar and molar abutments and the lower stresses in that of canine than rigid design. Alveolar bone loss increased the stresses in supporting bone of corresponding abutment. Conclusion: Careful evaluation of the retentive capacity of retainers and the periodontal condition of abutments may be required for the prosthetic design of fixed partial denture with a pier abutment.

Effects of Environmental Factors on Growth and Nitrogen Fixation Activity of Autumn Olive (Elaeognus umbellata) Seedlings (보리수나무 유식물의 생장과 질소고정 활성에 대한 환경요인의 영향)

  • 송승달
    • Journal of Plant Biology
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    • v.37 no.3
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    • pp.387-394
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    • 1994
  • Effects of environmental factors of light, temperature, nitrogen sources and water stress were analyzed quantitatively on the nodule formation and nitrogen fixation activity of autumn olive plant (Elaeagnu$ umbellala Thunb.) during the seedling growth. Seedlings showed the maximum nitrogenase activity of $72.5\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$ in the early nodulation stage. The relative growth rate and T/R ratio changed from $1.60%{\cdot}d^{-1}$ and 1.12 in the earlier stage to $3.75%{\cdot}d^{-1}$ and 2.31 in the later stage, respectively. light conditions of 20-25, 1015 and 4-6% resulted in decreases of 41, 54 and 71% of the nitrogenase activity, respectively. Nodules incubated in 15, 20, 25 and $30^{\circ}C$ showed the activities of 5.4, 24.7, 51.6 and $58.5\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$ respectively. Pretreatment with low temperature ($15^{\circ}C$) followed incubation at $30^{\circ}C$ attained higher nitrogenase activity ($66.5\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$) than that with higher temperature ($35^{\circ}C$). The oxygen pressure above 16 kPa is necessary for saturation of the nodule activity, but the activity was inhibited severely by physical impact such as the exision or isolation of nodules from the root. The relative activities of early nodules grown in pH 5.5, 6.5 and 8.0 were 89, 100 and 40% and those grown in 1 and 3 mM of $NO_3\;and\;NH_4$ were 6, 1 and 68, 50%, respectively. Watering levels of 20, 50 and 100 mL during the seedling growth resulted in 35, 120 and 8 mg of nodule formation and 33.6, 58.4 and $8.4\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$ of the nitrogenase activity, respectively. Water stress with 86% decrease of soil water content caused temporary wilting point of leaf and a complete disappearance of nitrogenase activity of nodules, though the water content and transpiration rate in plant were reduced to 90 and 53%, respectively.tively.

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Comparison of Quality Characteristics among Chilled Loins Obtained from Jeju Black Cattle, Hanwoo and Imported Australian Beef (냉장한 제주흑우육, 한우육 및 호주산 수입우육의 품질 특성 비교)

  • Moon, Yoon-Hee
    • Journal of the East Asian Society of Dietary Life
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    • v.22 no.4
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    • pp.497-505
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    • 2012
  • The nutritional, physicochemical and sensory quality characteristics of chilled loins of Jeju Black Cattle, Hanwoo and imported Australian beef were compared. For nutritional properties, moisture and crude protein contents of Jeju Black Cattle beef were 65.91% and 17.39% respectively, the lowest among the comparison groups. On the other hand, their crude fat and crude ash contents were 15.89% and 0.79%, respectively, the highest among the comparison groups. There was a significant difference in crude ash content, but there was no significant difference in the other properties. The Fe and Zn contents of Jeju Black Cattle beef were 29.85 and 48.13 ppm, respectively, lower than those of Hanwoo beef, although the difference was not significant. These values were higher than those of Australian beef (p<0.05). There was no significant difference among the samples in contents of Ca, P, Na and Cu. The total amino acid and essential amino acid contents of Jeju Black Cattle beef were 16.81% and 9.08%, respectively, the lowest among the samples, although the difference was not significant. The ratio of essential amino acids to total amino acids of Jeju Black Cattle beef was 54.01%, the highest among the samples. The saturated fatty acid (43.25%), stearic acid (13.51%), linoleic acid (2.39%) and essential fatty acid (2.47%) contents of Jeju Black Cattle beef were the lowest. However, their unsaturated fatty acid (56.69%), oleic acid (47.80%) and MUFA/SFA ratios were the highest, and there were significant differences compared with Australian beef. There was no significant difference in the PUFA/SFA ratio. Among physicochemical properties, redness ($a^*$) of Jeju Black Cattle beef was the highest while melting point was the lowest (p<0.05). There were no significant differences in lightness ($L^*$), yellowness ($b^*$), pH, water holding capacity, cooking loss or shear force value. For sensory properties, raw meat aroma, cooked meat aroma and palatability of Jeju Black Cattle beef were superior compared to other comparison groups, with significant differences in comparison with Australian beef (p<0.05). However, there were no significant differences in taste, juiciness, or tenderness of cooked meat.