• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.347-355
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• 2016

An exo-β-D-glucosaminidase (AorCsxA) from Aspergillus oryzae FL402 was heterologously expressed and purified. The deduced amino acid sequence indicated that AorCsxA belonged to glycoside hydrolase family 2. AorCsxA digested colloid chitosan into glucosamine but not into chitosan oligosaccharides, demonstrating exo-β-D-glucosaminidase (CsxA) activity. AorCsxA exhibited optimal activity at pH 5.5 and 50℃; however, the enzyme expressed in Pichia pastoris (PpAorCsxA) showed much stronger thermostability at 50℃ than that expressed in Escherichia coli (EcAorCsxA), which may be related to glycosylation. AorCsxA activity was inhibited by EDTA and most of the tested metal ions. A single amino acid mutation (F769W) in AorCsxA significantly enhanced the specific activity and hydrolysis velocity as revealed by comparison of V_max and k_cat values with those of the wild-type enzyme. The three-dimensional structure suggested the tightened pocket at the active site of F769W enabled efficient substrate binding. The AorCsxA gene was heterologously expressed in P. pastoris, and one transformant was found to produce 222 U/ml activity during the high-cell-density fermentation. This AorCsxA-overexpressing P. pastoris strain is feasible for large-scale production of AorCsxA.

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.1845-1854
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• 2016

The transglycosylation activity of amylosucrase (ASase) has received significant attention owing to its use of an inexpensive donor, sucrose, and broad acceptor specificity, including glycone and aglycone compounds. The transglycosylation reaction of recombinant ASase from Deinococcus radiopugnans (DRpAS) was investigated using various phenolic compounds, and quercetin-3-O-rutinoside (rutin) was found to be the most suitable acceptor molecule used by DRpAS. Two amino acid residues in DRpAS variants (DRpAS Q299K and DRpAS Q299R), assumed to be involved in acceptor binding, were constructed by site-directed mutagenesis. Intriguingly, DRpAS Q299K and DRpAS Q299R produced 10-fold and 4-fold higher levels of rutin transglycosylation product than did the wild-type (WT) DRpAS, respectively. According to in silico molecular docking analysis, the lysine residue at position 299 in the mutants enables rutin to more easily position inside the active pocket of the mutant enzyme than in that of the WT, due to conformational changes in loop 4.

• 대한지역사회영양학회지
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• 제8권6호
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• pp.867-875
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• 2003

The purpose of this study was to investigate the psychosocial factors influencing dairy product consumption of female university students in Daejeon. The Theory of Planned Behavior provided the basis for this study. As a result of the pilot-study, 18 behavioral beliefs, 8 normative beliefs, and 12 control beliefs were identified. The subjects (n=236) were grouped into a high-consumption group (1 serving/day, n=117) and a low consumption group (< 1 serving/day, n=119). The data were analyzed using t-tests or $\chi$$^2$-tests. Among the general characteristics, there were significant differences in the amount of pocket money spent per month, residence type (p<0.01), weight, frequency of exercise and perceived health status (p<0.05) of the subjects. With respect to the 18 behavioral beliefs, the high consumption group responded less negatively on ‘eating dairy foods would not be convenient’ than the low consumption group (p<0.05). None of the subjective normative items were significantly different between the two groups. However, notable differences were found in regard to the control beliefs (8 out of 12 control beliefs). These included overall control over consuming dairy products (p< 0.001), as well as specific beliefs regarding barriers such as easy spoilage of dairy products, the cost, eating them for snacks and dislike for them (p<0.05). In addition, specific situations, such as ‘when I want them I get them’ (p<0.01), eating out and the availability of dairy foods at home (p<0.05) were significantly different between the two groups. The high consumption group showed more control over these barriers or situations. These results suggest that nutritional education for young female adults should incorporate strategies to increase their perceived control over the consumption of dairy products by removing barriers and including environmental approaches which address the availability issues.

• Journal of Periodontal and Implant Science
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• 제34권3호
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• pp.647-657
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• 2004

A non-carious cervical lesion(NCCL) is the loss of tooth structure at the cementoenamel junction level that is unrelated to dental caries. This study was to evaluate the occlusal and periodontal status of teeth with non-carious cervical lesions. We evaluated 105 teeth with non-carious cervical lesions in 35 subjects aged 38-75 years and characterized them based on the shape and dimension, plaque retention, bleeding on probing(BOP), probing pocket depth(PPD), occlusal status, brushing type, hypersensitivity and wear facet. The results of this study were as follows 1. No significant association was observed between cervical lesions and occlusal contact in lateral excursions. 2. No significant difference occurred in plaque retention, PPD, BOP between teeth with and without cervical lesions. 3. Test teeth had a significantly higher percentage of hypersensitivity and occlusal wear facet than teeth without cervical lesions. 4. Wedge shaped lesions had a significantly higher percentage of plaque than saucer shaped lesions. 5. Teeth with plaque were found to have significantly deeper PPD than teeth without plaque retention in cervical regions. 6. Teeth with occlusal contacts were found to have significantly deeper PPD than teeth without occlusal contacts. 7. No significant association was observed between cervical lesions and PPD independent of plaque retention and occlusal contacts Although more knowledge is necessary, our results suggest that occlusal contact and bacterial plaque may influence on periodontal tissue, but NCCL is not directly associated with periodontal health

• Journal of Yeungnam Medical Science
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• 제31권2호
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• pp.113-116
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• 2014

A 52-year-old man was referred to our hospital due to fever and myalgia that occurred 2 weeks earlier. He showed a complete atrioventricular block on his electrocardiogram, and his vital signs were unstable. On his transthoracic echocardiograph, the 1.5 cm vegetation in the aortic valve with severe aortic regurgitation suggested infective endocarditis. His transesophageal enchocardiograph showed abscess in his mitral-aortic intervalvular fibrosa and vegetation was suspected on his anterior mitral valve leaflet. The patient underwent an emergent operation for valve replacement with temporary epicardial pacing. Intraoperatively, the septal leaflet of his tricuspid valve was injured during the debridement of the abscess pocket that was extended to the membranous septum. The aortic, mitral, and tricuspid mechanical valves were replaced with annular reconstruction without complications. After 14 days of intravenous antibiotics, we successfully changed the epicardial pacemaker into a transvenous DDD-type permanent pacemaker by placing a left ventricular lead via the coronary sinus and an atrial lead in the right atrium appendage. The patient was discharged in a tolerable state and was examined uneventfully in our hospital's outpatient clinic for 8 months.

• Elastomers and Composites
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• 제38권3호
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• pp.195-205
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• 2003

본 논문에서는 미드솔에 에어싸이클 펌프를 장착한 골프화에 대한 해석적 방법과 설계기술을 보여준다. 골프화는 유한요소법으로 모델링 하였으며, 더 나은 설계를 위해서 신발을 구성하는 미드솔과 아웃솔의 형상을 고려하였다. 또한 미드솔에 있는 에어싸이클 펌프의 최적의 크기와 형상을 조사하였다. 표준 인체 압력 값을 유한요소해석의 경계 값으로 채택하였다. 오그덴 타입의 변형률에너지 함수의 미지 상수는 인장시험에 따라서 조사하였다. 비선형해석을 위해서 상용 유한요소해석 프로그램 MARC V7.3을 이용하여 미드솔과 아웃솔의 변형률과 부피 변화 값을 각각 구하였다. 미드솔과 아웃솔 유한요소법에 의해 구해진 값은 탄성체의 특성에 따라서 달리 나타난다. 여기에 보인 결과는 보다 나은 골프화 해석에 사용될 것이다. 더욱이, 결과 간들은 보다 효과적인 방법을 개발하기 위한 방법으로 신발산업체에 사용되어 질 수 있다.

• The Journal of Advanced Prosthodontics
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• 제13권1호
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• pp.46-54
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• 2021

Purpose. The design of the implant-abutment complex is thought to be responsible for marginal bone loss (MBL) and might affect the condition of the peri-implant tissues. This the present study aimed to evaluate the influence of the implant-abutment complex on MBL and the peri-implant tissues in partially edentulous patients treated with dental implants and determine the most advantageous design. Materials and Methods. A total of ninety-one endosseous implants with different designs of implant-abutment complex [tissue level-TL (n = 30), platform switch-PS (n = 18), and platform match-PM (n = 43)] were reviewed for MBL, Probing Pocket Depth (PPD) and Bleeding on Probing (BoP). MBL was calculated for first year of the insertion and the following years. Results. The median MBL for the PM implants (2.66 ± 1.67 mm; n = 43) in the first year was significantly higher than those for the other types (P=.033). The lowest rate of MBL (0.61 ± 0.44 mm; n = 18) was observed with PS implants (P=.000). The position of the crown-abutment border showed a statistically significant influence (P=.019) and a negative correlation (r=-0.395) on MBL. BoP was found significantly higher in PM implants (P=.006). The lowest BoP scores were detected in PS implants, but the difference was not significant (P=.523). The relation between PPD and connection type revealed no statistically significant influence (P>.05). Conclusion. Within the limitations of the present study, it may be concluded that PS implants seem to show better peri-implant soft tissue conditions and cause less MBL.

• Biomolecules & Therapeutics
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• 제32권2호
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• pp.214-223
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• 2024

Metabolic abnormalities in the liver are closely associated with diverse metabolic diseases such as non-alcoholic fatty liver disease, type 2 diabetes, and obesity. The aim of this study was to evaluate the ameliorating effect of robinetin (RBN) on the significant pathogenic features of metabolic failure in the liver and to identify the underlying molecular mechanism. RBN significantly decreased triglyceride (TG) accumulation by downregulating lipogenesis-related transcription factors in AML-12 murine hepatocyte cell line. In addition, mice fed with Western diet (WD) containing 0.025% or 0.05% RBN showed reduced liver mass and lipid droplet size, as well as improved plasma insulin levels and homeostatic model assessment of insulin resistance (HOMA-IR) values. CD38 was identified as a target of RBN using the BioAssay database, and its expression was increased in OPA-treated AML-12 cells and liver tissues of WD-fed mice. Furthermore, RBN elicited these effects through its anti-histone acetyltransferase (HAT) activity. Computational simulation revealed that RBN can dock into the HAT domain pocket of p300, a histone acetyltransferase, which leads to the abrogation of its catalytic activity. Additionally, knock-down of p300 using siRNA reduced CD38 expression. The chromatin immunoprecipitation (ChIP) assay showed that p300 occupancy on the promoter region of CD38 was significantly decreased, and H3K9 acetylation levels were diminished in lipid-accumulated AML-12 cells treated with RBN. RBN improves the pathogenic features of metabolic failure by suppressing the p300-CD38 axis through its anti-HAT activity, which suggests that RBN can be used as a new phytoceutical candidate for preventing or improving this condition.

• 농촌의학ㆍ지역보건
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• 제24권2호
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• pp.233-243
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• 1999

본 연구는 노인들이 지각한 사회적 지지의 정도를 확인하고 노인의 일상생활활동과 상관관계를 분석함으로써 노인의 삶의 지을 향상시킬 수 있는 간호중재방안의 모색에 유용한 기초자료를 제공하기 위해 시도하였다. 본 연구는 대구와 경북에 거주하는 65세 이상의 재가노인으로 대구지역 120명과 경북지역 119명이며 자료수집 기간은 1998년 7월 20일부터 8월 30일 까지 이다. 연구도구로는 송미순(1991)의 지각된 사회적 지지 척도로서 정보적 지지 6문항, 물질적 지지 5문항, 감정적 지지 5문항, 자존감 지지 4문항으로 구성된 총 20문항과 Lawton과 Bordy(1971)의 수단적 일상생활활동(Instrumental Activities of Daily Living: IADL) 측정도구 8문항을 사용하였다. 자료 분석 방법은 SAS 프로그램을 이용하여 실수와 백분율, 평균과 표준편차, t-test, ANOVA, Pearson Correlation으로 분석하였다. 연구결과를 요약하면 다음과 같다. 1) 노인이 지각한 사회적 지지가 전체 평균점수 2.36점 이었으며, 이 중 물질적 지지가 2.52점으로 가장 높았고 자존감 지지가 2.18점으로 가장 낮았다. 2) 노인의 일상생활활동중에서 물건구입 평균점수 2.89점, 자신의 투약에 대한 책임 2.62점, 수송양식 2.22점 순으로 높았으며, 세탁일 평균점수 1.24점, 음식 준비 능력 1.58점 순으로 낮았다. 3) 노인이 지각한 사회적지지 중 자존감 지지(r=.58, p=.013), 정보적지지(r=.54, p=.048), 감정적지지(r=.41, p=.000), 그리고 물질적지지(r=.32, p=.000) 순으로 유의한 상관관계를 나타냈다. 4) 대상자의 일반적 특성에 따른 사회적지지 정도의 차이를 검증한 결과 유의한 차이를 보인 것은 연령(F=4.61, p=.004), 교육정도(F=4.04, p=.002), 배우자 유무(t=3.37, p=.000) 한달 용돈 정도(F=3.51, p=.032), 용돈에 대한 만족도(F=5.12, p=.007) 등으로 나타났다. 이상의 결과로 미루어 볼 때 사회적 지지가 노인의 일상생활활동에 영향을 미치는 중요한 요인이 되므로 특히 사회교육으로부터의 정보적지지, 용돈 등으로 부터의 물질적지지, 배우자나 사회망으로부터의 정서적 지지와 자존감 지지가 효과적인 지지가 되므로 노인을 중심심으로 한 사회망으로부터 사회적 지지를 고취시키고 우리나라 노인에게 알맞는 지지간호 중재 개발이 필요하다. 본 연구의 결과를 기초로 하여 다음의 제언을 하고 자 한다. 1)노인의 일상생활활동을 증진시키는 사회적지지 간호중재 프로그램의 개발에 대한 추후 연구가 필요하다. 2) 노인의 사회적 지지가 노인의 일상생활활동에 미치는 영향을 규명하는 유사실험연구를 시도할 것을 제언한다.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1433-1441
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• 2015

Pollution resulting from the discharge of textile dyes into water systems has become a major global concern. Because peroxidases are known for their ability to decolorize and detoxify textile dyes, the peroxidase activity of Vitreoscilla hemoglobin (VHb) has recently been studied. It is found that VHb and variants of this enzyme show great promise for enzymatic decolorization of dyes and may play a role in achieving their successful removal from industrial wastewater. The level of VHb peroxidase activity correlates with two amino acid residues present within the conserved distal pocket, at positions 53 and 54. In this work, sitedirected mutagenesis of these residues was performed and resulted in improved VHb peroxidase activity. The double mutant, Q53H/P54C, shows the highest dye decolorization and removal efficiency, with 70% removal efficiency within 5 min. UV spectral studies of Q53H/P54C reveals a more compact structure and an altered porphyrin environment (λ_Soret = 413 nm) relative to that of wild-type VHb (λ_Soret = 406), and differential scanning calorimetry data indicate that the VHb variant protein structure is more stable. In addition, circular dichroism spectroscopic studies indicate that this variant's increased protein structural stability is due to an increase in helical structure, as deduced from the melting temperature, which is higher than 90℃. Therefore, the VHb variant Q53H/P54C shows promise as an excellent peroxidase, with excellent dye decolorization activity and a more stable structure than wild-type VHb under high-temperature conditions.