• Journal of the Korean Chemical Society
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• v.34 no.2
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• pp.179-183
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• 1990

New synthesis and electroanthennogram (EAG) responses of the pheromones of the diamond back moth Plutella xylostella L. are described. Lithium dianion of propargyl alcohol was alkylated with 1-bromononane to give 2-dodecyn-1-ol, which was converted to 11-dodecyn-1-ol by acetylene-zipper reaction. Dianion of 11-dodecyn-1-ol was alkylated with 1-bromobutane to provide 11-hexadecyn-1-ol, which was in turn reduced over Pd/BaSO4 to afford (Z)-11-hexadecen-1-ol (3). (Z)-11-Hexadecen-1-ol was oxidized and acetylated to (Z)-11-hexadecen-1-al (1) and (Z)-11-hexadecen-1-yl acetate (2), respectively. The electroantennogram (EAG) responses of the diamond back moth to the pheromone compouds thus synthesized were measured.

• The Korean Journal of Pesticide Science
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• v.7 no.1
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• pp.38-44
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• 2003

In vitro inhibitory activity of 34 insecticides and 31 fungicides to glutathione-S-transferase and esterases extracted from rats was determined. Of tested pesticides, the pesticides with high activity on both detoxifying enzymes were mixed with pesticides that are known to be detoxified by detoxifying enzymes. Glutathione-S-transferase was inhibited by thiodicarb $(I_{50}:1.87\times10^{-4}M)$, thiocyclam $(7.40\times10^{-4}M)$, dithianon $(7.55\times10^{-5}M)$, and tolylfluanide $(8.66\times10^{-5}M)$, while esterases by dichlorvos $(8.95\times10^{-8}M)$, pirimicarb $(2.74\times10^{-6}M)$, pyrazophos $(3.31\times10^{-5}M)$, and benomyl $(4.96\times10^{-5}M)$. After acephate known to be detoxified by glutathione-S-transferase was mixed with glutathione-S-transferase-inhibiting pesticides and phenthoate known to be detoxified by esterases was mixed with esterases-inhibiting pesticides, insecticidal activities of such mixtures were determined against diamondback moth (PlutelLa xylostella L.). Synergistic action was observed in all pesticide combinations. The highest synergistic action was obtained when phenthoate was combined with dichlorvos, showing that co-toxicity coefficients were 1512 and 1877 after 24 and 48 hours of treatment, respectively. Several other combinations of pesticides, such as phenthoate with benomyl, and acephate with dithianon, also showed synergism, showing that their co-toxicity coefficients were about 1,000 and 500, after 24 hours of treatment, respectively. Our results showed that combinations of pesticides inhibited by detoxifying enzymes and ones detoxified by detoxifying enzymes resulted in increased toxicities of pesticides, suggesting that such combinations could be used to develop pesticide mixtures with more broad spectrum and high effectiveness.

• Plant Biotechnology Reports
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• v.5 no.3
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• pp.217-224
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• 2011

Non-heading Chinese cabbage (Brassica rapa L. ssp. chinensis) is a popular vegetable in Asian countries. The diamondback moth (DBM), Plutella xylostella (L.), an insect with worldwide distribution, is a main pest of Brassicaceae crops and causes enormous crop losses. Transfer of the anti-insect gene into the plant genome by transgenic technology and subsequent breeding of insect-resistant varieties will be an effective approach to reducing the damage caused by this pest. We have produced transgenic non-heading Chinese cabbage plants expressing the potato proteinase inhibitor II gene (pinII) and tested the pest resistance of these transgenic plants. Non-heading Chinese cabbages grown for 45 days on which buds had formed were used as experimental materials for Agrobacterium-mediated vacuum infiltration transformation. Forty-one resistant plants were selected from 1166 g of seed harvested from the infiltrated plants based on the resistance of the young seedlings to the herbicide Basta. The transgenic traits were further confirmed by the Chlorophenol red test, PCR, and genomic Southern blotting. The results showed that the bar and pinII genes were co-integrated into the resistant plant genome. A bioassay of insect resistance in the second generation of individual lines of the transgenic plants showed that DBM larvae fed on transgenic leaves were severely stunted and had a higher mortality than those fed on the wild-type leaves.

• Research in Plant Disease
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• v.9 no.2
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• pp.99-103
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• 2003

Bioactivities of Korean ginkgo (Ginkgo biloba L.) extract were investigated against several fungi, general bacteria and insect pests. Crude methanolic extracts of different parts of Korean ginkgo showed different bioactivities depending on the target organisms. The methanolic extract showed in vitro antimicrobial activity at dose of 200 ug per paper disc. The extract of ginkgo stalk was some higher than seed coat and root. The extract also showed a remarkable in vivo antifungal activity against green mold (Trichoderma harzianum) on compost surface of spawn bags and in vivo insecticidal activity to Nilaparvata lugens, Plutella xylostella and Tetranychus urticae. This study suggests that Korean ginkgo extracts have a potential as a natural pesticide.

• Korean Journal of Microbiology
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• v.46 no.4
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• pp.389-396
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• 2010

Insecticidal crystalline toxin producing Bacillus thuringiensis BT17 strain was isolated and identified as B. thuringiensis serovar colmeri by 16S rRNA analysis. BT17 strain produced crystalline ${\delta}$-endotoxin against to Lepidopteran larvae effectively on the culture broth of soybean meal and skim milk, $30^{\circ}C$ and 36 h shaking culture of 280 rpm. The maximum colony forming unit achieved when the culture was continued for 24 h, but the number of crystals increased until 36 h in the 200 L fermentor. Liquid type of biological insecticide product was made, and after 3 months storage in $20^{\circ}C$ the number of crystals was increased up to twice than beginning. Biocontrol effect of BT17 insecticide product was better in Plutella xylostella than in Spodoptera exigua, and the toxicity to animals was negligible.

• Korean journal of applied entomology
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• v.33 no.4
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• pp.242-249
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• 1994

These studles were conducted to investigate the development of chem~cal resistance and cross.resista. nce m dlarnondback moth (Piuteiia xylostella L). The resistance development of diamondback moth greatly vaned under single selection of five insectlades. The tnflumuron and lambda cyhalothnn strains at 8th selected generation showed 374- and 29.l.fold resistant levels. respectively, as compared with the susceptible strain. However, the Baciilus thuringiensis-seleded at 8th selected generatLon exhibited 240-fold resistant level. and the prothiophos-selected at 8th generation revealed 14.3-fold resistant !eve1 while the cartap hydrochloride-selected at 8th generailon showed 9.1-fold resistant level Prothiophos- selected strain showed low cross-resistance level to cartap hydrochloride, while this strain exhibited no cross-resistance of 1.3 to 2.8-fold to other msectlcides. Cartap hydrochlonde- seleded strain showed 19.9-fold. a high cross-resistance to lambda cyhalothrin, but this strain showed 2.2-34 fold, no cross resistance to other insecticide. Lambda cyhalothnn-selected strain exhibited cross-resistance to cartap hydrochlolide and prothiophas. Triflumuron-seleded strain showed 1.3-4.9 fold. no cross-resistance to other ~nsectic~dTe he B. ihuring~ensis-selededs train showed no cross-resistance to other insecticides.

• The Korean Journal of Pesticide Science
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• v.3 no.1
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• pp.13-19
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• 1999

Insecticidal potentials of polar and non-polar tractions obtained from 84 medicinal plants were screened against five major agricultural insects. Based on the primary and secondary screening results, non-polar fraction of Atractylodes koreana Kitam. rhizomes was selected to isolate and identify an active compound effective to diamondback moth (Plutella xylostella L.) larvae. Counter-current distribution separation on the non-polar fraction and TLC and spectroscopic analyses (GC-MS and $^{1}H$- and $^{13}C$-NMR) revealed that molecular formula of the active compound was $C_{15}H_{22}O$ known as a sesquiterpenoid 4,11-selinadien-3-one (${\alpha}$-cyperone). However, ${\alpha}$-cyperone was not detected in the non-polar fractions that showed high insecticidal potential against the diamondback moth. Although ${\alpha}$-cyperone has been first identified from Cyperus rotundus, the compound did not occur in C. rotundus cultivated in Korea.

• The Korean Journal of Pesticide Science
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• v.6 no.4
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• pp.271-278
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• 2002

Methanol extracts from 420 samples of 173 plant species in 58 families were tested at 5000 ppm for their insecticidal and acaricidal activities against five economically important arthropod pests by spray method. The responses varied with arthropod pest species, plant species and plant tissue sampled. In a test with Nilaparvata lugens Stal, extracts from Zanthoxylum piperitum barks, Chamaecyparis obtusa leaf and Quercus salicina leaf showed potent insecticidal activity. With Plutella xylostella L., potent larvicidal activity was observed from extracts of Platycarya strobilacea wood, Meliosma myriantha barks, Sophora japonica leaf, Zanthoxylum piperitum barks, and Pinus thunbergii wood. Methanol extracts of Sophora japonica leaf and Zanthoxylum piperitum barks showed high insecticidal activity against Spodoptera litura. In a test with Tetranychus urticae Koch, extract from Carpinus coreana leaf, Firmiana simplex barks, Elaeagnus macrophylla leaf, Aralia elata leaf, Comus controversa barks and Chamaecyparis obtusa leaf exhibited strong acaricidal activity. As a naturally occurring pest control agent, Zanthoxylum piperitum barks could be useful as new insecticidal and acaricidal products against various arthropod pests.

• Korean journal of applied entomology
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• v.31 no.4
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• pp.338-344
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• 1992

This test has been carried out to clear the effectiveness of pyrethroid insecticides to the Diamond back moth, that was complained by a farmer. The results obtained are summarized as follows. Effectiveness of Pyrethroids to suceptable strain and the results of chemical anaysis on the ingredients of pyrethroikds were normal at recommended concentrations. The effect ualue of pyrethroid by which the blocks were only one time treated was 57-59%, whereas the blocks sprayed 4 consecutive times of pyrethroid didn't show any effectiveness as shown in larval increasing rate of 489-552%. Among the results of field test conducted from 1990 to 1991 at 7 area, Dae Gu area showed the highest Effectiveness as 95-98%. However, Pyong Taek area, showed the least effectiveness as 0% in effect value. The resistant population which was collected at Tae kwan Ryung area was resurrected susceptability as much as JMC, in case of Placing it in non-treatment environment, whereas the pupulation which was revealed to pyrethroids for 6 genetations showed as much as 341-544 times of resistance compared with JMC. The non-effectiveness of pyrethroids to Diamondback moth which was complainted by a certain farmer named Mr Ju, was clearly resulted by the development of insect resistance. In Korea, the resistance of Diamondback moth to pyrethroids has developed in some area, as well as the resistance of pyrethroids has developed according to the continuous use of Insecticides, and the susceptability resurrected by stopping the use of pyrethroids. The result from this test would suggest that the pyrethroid insecticides should be applied in turb with other insecticides.

• The Korean Journal of Pesticide Science
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• v.5 no.2
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• pp.18-25
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• 2001

Ethanol extracts from 46 vegetables were tested their insecticidal activities toward five agricultural insect pests and four stored-product insect pests. The efficacy varied with both agricultural insects/stored-product insects and vegetable species used. Potent insecticidal activities, at the concentration of 5,000 ppm, were produced from extracts of Nelumbo nucifera and Ulva lactuca against Myzus persicae, Zea ways and Z. mays (leaf) against Nilaparvata lugens, Citrullus vulgaris (seed) and U. lactuca against Plutella xylostella, N. nucifera, Z. mays, and Z. mays (leaf) against Spodoptera litura, and C. vulgaris (seed), Daucus carota, Helianthus annuus (leaf), H. annuus (flower), Lactuca sativa, and Zingiber officinale against Tetranychus urticae. Potent insecticidal activities at the concentration of 2,500 ppm were exhibited from the extracts of N. nucifera and U. lactuca against M. persicae, Z. mays against N. lugens, C. vulgaris (seed) and U. lactuca against xylostella, N. nucifera and Z. mays against S. litura, and C. vulgaris (seed), H. annuus (flower), and L. santiva against T. urticae. Against four stored-product insect pests at 50 ppm, extracts of C. vulgaris (seed) and Cucurbita moschatla (seed) against Sitophilus oryzae and C. vulgaris (seed), H. annuus (seed), and Z. officinale against Plodia interpunctellfa revealed potent insecticidal activities over 80% mortality. In tests with Callosobruchus chininsis and Lasioderma serricorne, extracts of all vegetables tested exhibited meager and no activity.