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Purification and Characterization of a Bacteriocin, BacBS2, Produced by Bacillus velezensis BS2 Isolated from Meongge Jeotgal

  • Perumal, Venkatesh;Yao, Zhuang;Kim, Jeong A;Kim, Hyun-Jin;Kim, Jeong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.29 no.7
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    • pp.1033-1042
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    • 2019
  • Bacillus velezensis BS2 was isolated from meongge (common sea squirt) jeotgal, a Korean fermented seafood, and produces a bacteriocin, BacBS2, which strongly inhibits Listeria monocytogenes and Bacillus cereus. BacBS2 was partially purified by Q-Sepharose column chromatography after ammonium sulfate precipitation of the culture supernatant, then further purified by Sephadex G-50 column chromatography. Partially purified BacBS2 was estimated to be 6.5 kDa in size by Tricine-SDS PAGE and activity detection by gel-overlay. Enzyme treatment and FT-IR spectrum of partially purified BacBS2 confirmed its proteinaceous nature. BacBS2 was fully stable at pH 4-9, and half of activity was retained at pH 1-3. Full activity was retained after exposure to $80^{\circ}C$ for 15 min, but half of the activity was retained upon exposure to $90^{\circ}C$ for 15 min or $100^{\circ}C$for 10 min. BacBS2 inhibited L. monocytogenes by bactericidal mode of action. B. velezensis BS2 and its BacBS2 seem useful as biopreservatives for fermented foods such as jeotgal.

Proposal and Evaluation of the Safety Inspection Cost Estimation Model for Multi-building Construction Project (군집시설물 건설공사의 안전점검 대가 산정모델 제안 및 평가)

  • Kim, Jin-Won;Bang, Jong-Dae;Sohn, Jeong-Rak
    • Journal of the Architectural Institute of Korea Structure & Construction
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    • v.33 no.12
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    • pp.11-18
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    • 2017
  • The safety inspection cost of the construction work was based on commercial facilities classified as a single building. Therefore, it is not possible to fully reflect the characteristics of the multi-building construction project such as apartment houses. Therefore, this study suggests a reasonable estimation model that can fully reflect the characteristics of the multi-building construction project. The safety inspection cost estimation model proposed two models such as construction cost ratio method and cost plus fixed fee method. And these models were simulated by the apartment construction work and compared with the current standard. As a result, the current construction cost ratio method has shown that the safety inspection cost tends to be overestimated as the construction size increases. Therefore, the proposed model has reflected characteristics of the multi-building construction project, so that it can reasonably estimate the safety inspection cost more than the current standard.

Control of Weld Pool Size in GMA Welding Process Using Neural Networks (신경회로를 이용한 GMA 용접 공정에서의 용융지의 크기 제어)

  • 임태균;조형석;부광석
    • Journal of Welding and Joining
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    • v.12 no.1
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    • pp.59-72
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    • 1994
  • This paper presents an on-line quality monitoring and control method to obtain a uniform weld quality in gas metal arc welding (GMAW) processes. The geometrical parameters of the weld pool such as the top bead width and the penetration depth plus half back width are utilized to assess the integrity of the weld quality. Since a good quality weld is characterized by a relatively high depth-to-width ratio in its dimensions, the second geometrical parameter is regulated to a desired one. The monitoring variables are the surface temperatures measured at various points on the top surface of the weldment which are strongly related to the formation of the weld pool The relationship between the measured temperatures and the weld pool size is implemented on the multilayer perceptrons which are powerful for realization of complex mapping characteristics through training by samples. For on-line quality monitoring and control, it is prerequisite to estimate the weld pool sizes in the region of transient states. For this purpose, the time history of the surface temperatures is used as the input to the neural estimator. The control purpose is to obtain a uniform weld quality. In this research, the weld pool size is directly regulated to a desired one. The proposed controller is composed of a neural pool size estimator, a neural feedforward controller and a conventional feedback controller. The pool size estimator predicts the weld pool size under growing. The feedforward controller compensates for the nonlinear characteristics of the welding process. A series of simulation studies shows that the proposed control method improves the overall system response in the presence of changes in torch travel speed during GMA welding and guarantees the uniform weld quality.

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Studies on the Nutrient Contents and Optimum Prices of Eggs in Relation to Egg Weight (계란의 란중별영양성분 및 적정가격에 관한 조사연구)

  • 이규호;오봉국;오세정;이상진
    • Korean Journal of Poultry Science
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    • v.12 no.1
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    • pp.39-44
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    • 1985
  • These studies were conducted to compare the nutritive values and optimum prices of eggs among 6 groups of different egg Weight. With the total of 100 eggs of each weight group, after the weight percentage of egg yolk, albumen and shell in the whole egg were investigated, protein and fat contents of e99 yolk and albumen were analyzed. and then protein and fat contents in the whole eggs were calculated. Finally, the optimum prices of eggs in relation to the egg weight were studied on the basis of egg weight, protein content and protein plus fat contents of eggs, respectively. The results obtained are summarized as follows; 1. As the egg weight (X, g/10 eggs) increased, egg yolk (Y$_1$, %) and shell(Y$_2$, %) percentages tended to decrease, but egg albumen(Y$_3$, %) percentage increased lineally; Y$_1$=44.34-0.02X, Y$_2$=15.358-0.006 X, and Y$_3$=40.136+0.026 X. 2. There were no significant differences in protein and fat contents of eggs among 6 different groups of egg weight. 3. Protein (Y$_1$, %), fat (Y$_2$, %) and protein plus fat (Y$_3$, %) contents in the whole eggs declined progressively as the egg weight (X, g/10 eggs) increased ; Y$_1$=11.943-0.00032X, Y$_2$=13.996-0.00614X, and Y$_3$=25.939-0.00646X. 4. Similar results were obtained whether the optimum prices of eggs were estimated on the basis of egg weight or protein content of eggs, and they were higher in the large size eggs and lower in the small size eggs than the optimum prices of eggs estimated on the basis of protein plus fat content of eggs.

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Mechanism on Extraction of Heavy Metals from Soil by Ultrasonication (초음파 조사에 의한 토양내 중금속 추출 기작 연구)

  • Shin, Yeon-Jun;Lee, Cha-Dol;Yoo, Jong-Chan;Yan, Jung-Seok;Kim, Ho-Sub;Baek, Kitae
    • Journal of Soil and Groundwater Environment
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    • v.20 no.1
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    • pp.28-35
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    • 2015
  • In this study, the mechanisms on ultrasonication enhanced metals extraction were investigated compared with the conventional washing technique. We hypothesized the mechanisms on enhanced extraction of ultrasonication: ultrasonication increased the temperature of soil slurry and decreased average particle size of soil due to breakdown of soil aggregate. Actually, the ultrasonication increased the temperature of soil slurry to $60^{\circ}C$ in this study, and the increase in the temperature enhanced the metal extraction to 15-20% even in the conventional simple mixing. The conventional washing technique decreased average size of soil particles because of breakdown of soil aggregate, and the ultrasonication decreased the size more than that of washing. The breakdown of soil aggregate improved the contact between metals and washing agent, which enhanced the extraction of metals in the ultrasonication. Therefore, we concluded that the main mechanisms of ultrasonication are increase in the temperature and breakdown of the soil aggregate. Finally, the ultrasonicaiton increased the extractability of metals upto 40% compared to conventional washing technique.

Microwave Imaging of a Large High Contrast Scatterer by Using the Hybrid Algorithm Combining a Levenberg-Marquardt and a Genetic Algorithm (Levenberg-Marquardt와 유전 알고리듬을 결합한 잡종 알고리듬을 이용한 거대 강산란체의 초고주파 영상)

  • 박천석;양상용
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.8 no.5
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    • pp.534-544
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    • 1997
  • The permittivity distribution of a two-dimensional high-contrast object with large size, which leads to the global minimum of cost function, is reconstructed by iteratively using the hybrid algorithm of Levenberg-magquardt algorithm(LMA) plus Genetic Algorithm(GA). The scattered fields calculated in a cost function are expanded in angular spectral modes, of which only effective propagating modes are used. The definition of cost function based on the effective propagating modes enables us to formulate the minimum number of incident waves for the reconstruction of object. It is numerically shown that LMA has an advantage of fast convergence but can't reconstruct a high-contrast object with large size and GA can reconstruct a high-contrast object with large size but has an disadvantage of slow convergence, whereas an inverse scattering technique using the hybrid algorithm adopts only advantages of LMA and GA.

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Sizing System for Medical Compression Stockings -Focus on Imported Medical Compression Stockings in the Korean Market- (의료용 압박스타킹의 사이즈 체계에 관한 연구 -한국 시장에서의 수입 의료용 압박스타킹을 중심으로-)

  • Do, Wol-Hee;Kim, Nam-Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.36 no.8
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    • pp.860-874
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    • 2012
  • This study provides size information on current imported medical compression stockings in Korea. A total 20 brands from 6 countries imported and sold in the Korean on-off line markets were selected for this study and the sizing system of medical compression stockings was researched. The results of this study were as follows: there were size differences among different USA brands according to product type or compression class of medical compression stockings. To establish the sizing system for medical stockings of the German brands, the basic body measurements standard was set as cA, cY, cB, cB, cC, cD, cE, cF, cG, cH, cT, lD, lG, and lT. Italian brands developed the sizing system for medical compression stockings by compression class. In the Swiss brands, size types were sub-divided as 'Normal', 'Plus', 'Short', and 'Long'. The sizing 1 were confined within very narrow limits. There were differences of the basic body measurements between Taiwan brands. The results of the comparison of the size range of the imported brands with Size Korea (KATS, 2010) by two-way size distribution shows that the sizing systems of the imported brands were unsuitable for Koreans. To enhance the suitability of the sizing system for medical compression stockings, a new sizing system for Korean adult males and females needs to be established.

Diagnosis of fibroadenoma using radiolabeled bladder cancer specific peptide

  • Ha, Yeong Su;Kwak, Wonjung;Lee, Hwa Young;Lee, Byung-Heon;Hong, Il-Hwa;Jeong, Kyu-Shik;An, Gwang Il;Yoo, Jeongsoo
    • Journal of Radiopharmaceuticals and Molecular Probes
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    • v.1 no.1
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    • pp.38-45
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    • 2015
  • Most tumors are believed to overexpress several receptors, and small peptides targeting these receptors were developed for diagnosis and tumor therapy during past decade. Here we report that fibroadenoma can be visualized by bladder cancer specific peptide. A 9-mer bladder cancer specific peptide, which was discovered from the phage display method, was synthesized by peptide synthesizer, and additional tyrosine was conjugated at the N-terminal for radioiodination (Y-BP). Y-BP was radiolabeled with $^{131/124}I$ using Iodogen tube. The rat treated with N-butyl-N-(4-hydroxybutyl)nitrosamine for 8 weeks was allowed to grow until large size tumor was developed under axilla. The tumor model was microPET imaged sequentially using [$^{18}F$]FDG and radioiodinated $^{124}I-Y-BP$. The tumor was excised and examined by immunostaining studies. Radioiodinated $^{124}I-Y-BP$ was purified using fast protein liquid chromatography (FPLC) in > 90% radiochemical purity. The whole tumor was well visualized by [$^{18}F$]FDG with several intense focal uptake within tumor. The tumor was also clearly seen with $^{124}I-Y-BP$ at 4 h post-injection, and to our surprise the tumor uptake of $^{124}I-Y-BP$ lasted up to three days. The tumor was diagnosed histologically as a fibroadenoma derived from mammary gland. In conclusion, the bladder cancer specific peptide showed the good potential as a new radiotracer for the detection of breast fibroadenoma.

Effects of Frequency of Meals on Energy Utilization and Body Composition of Sheep Ingesting Diets of Equal Amount (급식회수(給食回數)가 면양(緬羊)의 열량대사(熱量代謝) 및 조직(組織)의 화학적성분(化學的成分)에 미치는 영향)

  • Han, In-K.
    • Applied Biological Chemistry
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    • v.8
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    • pp.21-28
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    • 1967
  • Two experiments with 32 sheep were conducted to study the effects of feeding the same amount of diet per day at different meal frequencies on ration digestibility, energy utilization, rate of gain, body composition and efficiency of gain. The results obtained are as follows: (1) The ingestion by sheep of the same amount of feed per day in 8 meals, 1 meal plus 7 ruminal inflations-deflations, and in 1 meal caused no different effect in the digestibility of the nutrients and energy, or the ME value of the diet. (2) Heat production per unit of metabolic size per unit of dietary intake was markedly lower for sheep ingesting 8 meals or administered 1 meal plus 7 ruminal inflations-deflations per day than for sheep fed 1 meal per day. (3) Body weight gain was significantly greater by sheep fed 8 meals per day or 1 meal plus 7 ruminal inflations-deflations than by those fed 1 meal per day. However, the gain in DM and energy of wool was not affected by frequency o( meals. (4) Sheep ingesting 8 meals or administered 1 meal plus 7 ruminal inflations-deflations per day gained body protein, fat and energy at a more rapid and efficient rate than sheep fed 1 meal per day. (5) Sheep fed 8 meals per day gained greater proportion of fat, protein and ash in the gained portion of the bodies than did 1 meal fed sheep. (6) An attempt was made to establish the possible explanations by which the frequency of ingesting meals exerts its effects.

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COLOR DIFFERENCE OF THE DENTAL COMPOSITES MEASURED BY DIFFERENT COLOR MEASURING INSTRUMENTS (복합레진 색상의 측정 기기에 따른 차이)

  • Park, Su-Jung;Noh, Eun-Young;Cho, Hyun-Gu;Hwang, Yun-Chan;Oh, Won-Mann;Hwang, In-Nam
    • Restorative Dentistry and Endodontics
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    • v.34 no.3
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    • pp.199-207
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    • 2009
  • The objective of this study was to evaluate the effect of color measuring instrument by measuring the color of dental composite resins. Nine shade light cured composite resin disks were prepared (diameter : 15 mm, thickness : 4 mm). CIE $L^*a^*b^*$ color scale of each disk was measured with 3 different types of spectrophotometer [MiniScan XE plus (Model 4000S, Hunter Lab, USA), CM-3500d (Minolta, Japan) and Specbos 2100 Miniature VIS Reflection spectrometer (Serial No: 319416, JETI Technishe VIS Instrumentic GmbH. Germany)]. Miniscan XE Plus and CM-3500d using identical measuring geometry with different size of viewing aperture. But Specbos 2100 using different measuring geometry. Within the limitation of this study, there were color difference (${\Delta}E^*$) from 2.4 to 7.8 between Miniscan XE Plus and CM-3500d, but $L^*$, $a^*$, $b^*$ values showed the high correlation. However, there were great color difference (${\Delta}E^*$) in the extent of about 20 between instruments with the different measuring geometry. Therefore, color scale measured by color measuring instrument should be used as a relative value rather than an absolute value in the field of dentistry.