• Journal of Animal Reproduction and Biotechnology
• /
• v.38 no.4
• /
• pp.275-290
• /
• 2023

Background: Porcine pluripotent stem cells (pPSCs) would provide enormous potential for agriculture and biomedicine. However, authentic pPSCs have not established yet because standards for pPSCs-specific markers and culture conditions are not clear. Therefore, the present study reports comparative pluripotency characteristics in porcine induced pluripotent stem cells (piPSCs) derived from different viral transduction and reprogramming factors [Lenti-iPSCs (OSKM), Lenti-iPSCs (OSKMNL) and Sev-iPSCs (OSKM)]. Methods: Porcine fibroblasts were induced into Lenti-iPSCs (OSKM) and Lenti-iPSCs (OSKMNL) by using Lentiviral vector and Sev-iPSCs (OSKM) by using Sendaiviral vector. Expressions of endogenous or exogenous pluripotency-associated genes, surface marker and in vitro differentiation in between Lenti-piPSCs (OSKM), Lenti-iPSCs (OSKMNL) and Sev-piPSCs (OSKM) were compared. Results: Colonial morphology of Lenti-iPSCs (OSKMNL) closely resembles the naïve mouse embryonic stem cells colony for culture, whereas Sev-iPSCs (OSKM) colony is similar to the primed hESCs. Also, the activity of AP shows a distinct different in piPSCs (AP-positive (+) Lenti-iPSCs (OSKMNL) and Sev-iPSCs (OSKM), but AP-negative (-) Lenti-iPSCs (OSKM)). mRNAs expression of several marker genes (OCT-3/4, NANOG and SOX2) for pluripotency was increased in Lenti-iPSCs (OSKMNL) and Sev-iPSCs (OSKM), but Sev-iPSCs (OSKM). Interestingly, SSEA-1 of surface markers was expressed only in Sev-iPSCs (OSKM), whereas SSEA-4, Tra-1-60 and Tra-1-81 were positively expressed in Lenti-iPSCs (OSKMNL). Exogenous reprogramming factors continuously expressed in Lenti-iPSCs (OSKMNL) for passage 20, whereas Sev-iPSCs (OSKM) did not express any exogenous transcription factors. Finally, only Lenti-iPSCs (OSKMNL) express the three germ layers and primordial germ cells markers in aggregated EBs. Conclusions: These results indicate that the viral transduction system of reprograming factors into porcine differentiated cells display different pluripotency characteristics in piPSCs.

• Proceedings of the Korean Society of Embryo Transfer Conference
• /
• 2002.06a
• /
• pp.1-16
• /
• 2002

• 대한생식의학회:학술대회논문집
• /
• 2004.06a
• /
• pp.51-51
• /
• 2004

• The Korea Genome Conference
• /
• 2002.08a
• /
• pp.50.1-50.1
• /
• 2002

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.02a
• /
• pp.3-3
• /
• 2003

No Abstract, See Full Text

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.02a
• /
• pp.4-4
• /
• 2003

No Abstract, See Full Text

• Journal of Genetic Medicine
• /
• v.9 no.2
• /
• pp.67-72
• /
• 2012

The generation of induced pluripotent stem cells (iPSCs) derived from patients' somatic cells provides a new paradigm for studying human genetic diseases. Human iPSCs which have similar properties of human embryonic stem cells (hESCs) provide a powerful platform to recapitulate the disease-specific cell types by using various differentiation techniques. This promising technology has being realized the possibility to explore pathophysiology of many human genetic diseases at the molecular and cellular levels. Furthermore, disease-specific human iPSCs can also be used for patient-based drug screening and new drug discovery at the stage of the pre-clinical test in vitro. In this review, we summarized the concept and history of cellular reprogramming or iPSC generation and highlight recent progresses for disease modeling using patient-specific iPSCs.

• The Korean Journal of Physiology and Pharmacology
• /
• v.16 no.1
• /
• pp.1-9
• /
• 2012

Because the average human life span has recently increased, the number of patients who are diagnosed with neurodegenerative diseases has escalated. Recent advances in stem cell research have given us access to unlimited numbers of multi-potent or pluripotent cells for screening for new drugs for neurodegenerative diseases. Neural stem cells (NSCs) are a good model with which to screen effective drugs that increase neurogenesis. Recent technologies for human embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) can provide human cells that harbour specific neurodegenerative disease. This article discusses the use of NSCs, ESCs and iPSCs for neurodegenerative drug screening and toxicity evaluation. In addition, we introduce drugs or natural products that are recently identified to affect the stem cell fate to generate neurons or glia.

• KSBB Journal
• /
• v.28 no.5
• /
• pp.269-274
• /
• 2013

Motor neuron disease (MND) is a fatal neurodegenerative disorder caused by progressive and selective degeneration of motor neurons (MNs). Because of the versatile nature, stem cells have the potential to repair or replace the degenerated cells. In this review, we discussed stem cell based therapies including the use of embryonic stem cells (ESCs), neural stem cells (NSCs), induced pluripotent stem cells (iPSCs) and genetically engineered cells to produce the neurotrophic factors for the treatment of MND. To achieve this goal, the knowledge of specificity of the cell target, homing and special markers are required.

• Biomedical Science Letters
• /
• v.19 no.1
• /
• pp.25-31
• /
• 2013

Electrospun nanofibers are being widely used as a substratum for mammalian cell culture owing to their structural similarity to collagen fibers found in extracellular matrices of mammalian cells and tissues. Especially, development of diverse synthetic polymers has expanded use of electrospun nanofibers for constructing cell culture substrata. Synthetic polymers have several benefits comparing to natural polymer for their structural consistency, low cost, and capability for blending with other polymers or small molecules to enhance their structural integrity or add biological functions. PMGI (polymethylglutarimide) is one of the synthetic polymers that produced a rigid nanofiber that enables incorporation of small molecules, peptides, and gold nanoparticles through co-electrospinning process, during which the materials are fixed without any chemical modifications in the PMGI nanofibers by maintaining their activities. Using the phenomenon of PMGI nanofiber, here I introduce a construction method of a nanofiber substratum having cell-affinity function towards a pluripotent stem cell by incorporating a small molecule in the PMGI nanofiber.