Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지

Construction of a Cell-Adhesive Nanofiber Substratum by Incorporating a Small Molecule

  • Jung, Dongju (Institute for Integrated Cell - Material Sciences (WPI-iCeMS), Yoshida Ushinomiya-cho Sakyo-ku, Kyoto University)
  • Received : 2013.01.16
  • Accepted : 2013.03.09
  • Published : 2013.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Electrospun nanofibers are being widely used as a substratum for mammalian cell culture owing to their structural similarity to collagen fibers found in extracellular matrices of mammalian cells and tissues. Especially, development of diverse synthetic polymers has expanded use of electrospun nanofibers for constructing cell culture substrata. Synthetic polymers have several benefits comparing to natural polymer for their structural consistency, low cost, and capability for blending with other polymers or small molecules to enhance their structural integrity or add biological functions. PMGI (polymethylglutarimide) is one of the synthetic polymers that produced a rigid nanofiber that enables incorporation of small molecules, peptides, and gold nanoparticles through co-electrospinning process, during which the materials are fixed without any chemical modifications in the PMGI nanofibers by maintaining their activities. Using the phenomenon of PMGI nanofiber, here I introduce a construction method of a nanofiber substratum having cell-affinity function towards a pluripotent stem cell by incorporating a small molecule in the PMGI nanofiber.

Keywords

References

  1. Dongju J, Minami I, Patel S, Lee J, Jiang B, Yuan Q, Li L, Kobayashi S, Chen Y, Lee KB, Nakatsuji N. Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells. Journal of Nanobiotechnology 2012. 10: 23. https://doi.org/10.1186/1477-3155-10-23
  2. Greiner A, Wendorff JH. Electrospinning: a fascinating method for the preparation of ultrathin fibers. Angewandte Chemie International Edition English 2007. 46: 5670-5703. https://doi.org/10.1002/anie.200604646
  3. Hansma HG, Pietrasanta L. Atomic force microscopy and other scanning probe miscroscopies. Current Opinion in Chemical Biology 1998. 2: 579-584. https://doi.org/10.1016/S1367-5931(98)80086-0
  4. Hoshino M, Tsujimoto T, Yamazoe S, Uesugi M, Terada S. Adhesamine, a new synthetic molecule, accelerates differentiation and prolongs survival of primary cultured mouse hippocampal neurons. Biochemical Journal 2010. 427: 297-304. https://doi.org/10.1042/BJ20100071
  5. Khil MS, Bhattarai SR, Kim HY, Kim SZ, Lee KH. Novel fabricated matrix via electrospinning for tissue engineering. Journal of Biomedical Materials Research B: Applied Biomaterials 2005. 72: 117-124.
  6. Klim JR, Li L, Wrighton PJ, Piekarczyk MS, Kiessling LL. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nature Methods 2010. 7: 989-994. https://doi.org/10.1038/nmeth.1532
  7. Koivunen E, Wang B, Ruoslahti E. Phage libraries displaying cyclic peptides with different ring sizes: ligand specificities of the RGD-directed integrins. Biotechnology (N Y) 1995. 13: 265-270. https://doi.org/10.1038/nbt0395-265
  8. Liu L, Yuan Q, Shi J, Li X, Jung D, Wang L, Yamauchi K, Nakatsuji N, Kamei K, Chen Y. Chemically-defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions. Biotechnology Letters 2012, doi:10.1007/s10529-012-0973-9.
  9. Melkoumian Z, Weber JL, Weber DM, Fadeev AG, Zhou Y, Dolley-Sonneville P, Yang J, Qiu L, Priest CA, Shogbon C, Martin AW, Nelson J, West P, Beltzer JP, Pal S, Brandenberger R. Synthetic peptide-acrylate surfaces for long-term selfrenewal and cardiomyocyte differentiation of human embryonic stem cells. Nature Biotechnology 2010. 28: 606-610. https://doi.org/10.1038/nbt.1629
  10. Pham QP, Sharma U, Mikos AG. Electrospinning of polymeric nanofibers for tissue engineering applications: a review. Tissue Engineering 2006. 12: 1197-1211. https://doi.org/10.1089/ten.2006.12.1197
  11. Ruoslahti E. RGD and other recognition sequences for integrins. Annual Review of Cell and Developmental Biology 1996. 12: 697-715. https://doi.org/10.1146/annurev.cellbio.12.1.697
  12. Ruoslahti E, Pierschbacher MD. New perspectives in cell adhesion: RGD and integrins. Science 1987. 238: 491-497. https://doi.org/10.1126/science.2821619
  13. Shi J, Wang L, Chen Y. Microcontact printing and lithographic patterning of electrospun nanofibers. Langmuir 2009. 25: 6015-6018. https://doi.org/10.1021/la900811k
  14. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007. 131: 861-872. https://doi.org/10.1016/j.cell.2007.11.019
  15. Vasita R, Katti D. Nanofibers and their applications in tissue engineering. International Journal of Nanomedicine 2006. 1: 15-30. https://doi.org/10.2147/nano.2006.1.1.15
  16. Yamazoe S, Shimogawa H, Sato S, Esko JD, Uesugi M. A dumbbell-shaped small molecule that promotes cell adhesion and growth. Chemistry & Biology 2009. 16: 773-782. https://doi.org/10.1016/j.chembiol.2009.06.008
  17. Yeom YI, Fuhrmann G, Ovitt CE, Brehm A, Ohbo K, Gross M, Hübner K, Schöler HR. Germline regulatory element of Oct-4 specific for the totipotent cycle of embryonal cells. Development 1996. 122: 881-894.
  18. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007. 318: 1917-1920. https://doi.org/10.1126/science.1151526