• Title/Summary/Keyword: Plum

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The Effects of Plum Extracts on the Proliferation of Human Epithelial Cell and Human Cervical Cancer Cells (자두 추출물이 인체 상피세포와 자궁경부암세포의 증식에 미치는 효과)

  • Han, Man-Deuk;Kweon, Dur-Han;Kang, Byung-Tae;Lee, Jae-Woo;Yoon, Ok-Hyun
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.5
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    • pp.710-718
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    • 2007
  • This study was designed to investigate the effects of plum(Prunus salicina Lindl. cultivars 'Oishiwase', 'Formosa', and 'Soldam') extracts on the proliferation as well as inhibition of human epithelial cells(HaCaT), human cervical carcinoma (HeLa, SiHa, and C33A) cells, and human stomach adenocarcinoma(SNU 638) cells. Dried plum was sequentially extracted and fractionated by hexane(KC-01), chloroform(KC-02), ethyl acetate(KC-03), n-butanol(KC-04), water(KC-05), methanol(KC-6), and hot water extract(KC-07). The epithelial and cancer cells were exposed for 48 h to $50{\mu}g/mL$ of plum extract in vitro, and were then analysed by a sulforhodamin B(SRB) staining assay. The methanol extract(KCP-6) of 'Formosa' proliferated not only the HaCaT cells(147.3%), but also the cervical carcinoma C33A cells(167.8%). The ethyl acetate extract of 'Soldam'(KCJ-3) significantly reduced the proliferation rate of the HPV positive conical carcinoma cells, at 61.5% for the SiHa cells and 70.5% for the HeLa cells. In the C33A cells, which are HPV negative cervical carcinoma cells, the hexane fractions of 'Formosa'(KCP-1) and 'Oishiwase'(KCD-1) markedly suppressed proliferation activity at 20.4% and 61.7%, respectively. However, the proliferation rate of the normal epithelial cells(HaCaT cell) was not reduced the proliferation rate by KCJ-3, KCP-1, or KCD-1, There were no significant effects on proliferation of the stomach cancer cells(SNU 638) by any of the extracts or fractions of the plum cultivars. These results suggest that the anti-proliferative effects of the plum cultivars were selective to the cancer cell origin. In conclusion, we found that several plum cultivar extracts, especially, the ethyl acetate fraction of 'Soldam" and the hexane fraction of "Formosa', have anti-proliferative activity toward human cervical carcinoma cells. However, further investigation is needed to assess the molecular mechanisms that mediate the antiproliferation activities of the plum cultivars.

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Quality, Safety and Sensory Characteristics of Plum Jangachi Produced using Automatic Plum Sarcocarp Separator (매실 과육 자동 분리기를 이용하여 제조한 매실장아찌의 품질, 안전성 및 관능특성)

  • Lee, Sang-Yoon;Park, Woo-Jun;Kim, Hyuck-Joo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.2
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    • pp.368-377
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    • 2021
  • Plum is a typical fruit that is consumed processed rather than raw. In this study, we manufactured one of the processed foods, viz., plum Jangachi. In this process, the manpower-dependent seed separation and flesh cutting operations were automated by mechanizing, thereby altering the manufacturing process. Quality and Safety were assessed through microbial evaluation, analysis of color, and detection of preservatives in the plum Jangachi. Preference factors were identified through sensual evaluation. When compared with other plum Jangachi currently available in the market, our product was determined to contain 2.7±0.1 Log CFU/g total aerobic bacteria, which is slightly higher than the average of other products. This was not surprising, as the figures are due to the inherent characteristics, which were determined to be lower as compared to other commercial plum Jangachi. Other coliforms, tar dyes, and preservatives were undetected, thereby conferring satisfactory Quality and Safety. In general, there was no statistical difference in the sensual evaluation and appearance; overall, our product received better feedbacks than products on the market. Taken together, our results provide a foundation for applying the mechanization of plum-processed foods, thereby promoting the local economy in the main production area, and overall characteristics obtained are regarded sufficient in terms of market competitiveness.

Dominance and Distribution of Weed Occurrence on Orchards of Apple, Grape, Peach, Pear, and Plum of Gyeongbuk Province (경북 지역의 사과, 배, 복숭아, 포도, 자두과원의 잡초 발생 분포 및 우점도)

  • Kim, Sang-Kuk;Shin, Jong-Hee;Kim, Se-Jong
    • Weed & Turfgrass Science
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    • v.5 no.2
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    • pp.51-59
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    • 2016
  • The study was conducted to get a information on the dominance and distribution of weeds occurred in major orchards including apple, grape, peach, pear, and plum at 631 sites of Gyeongsangbuk-do during winter and summer season. The weeds classified by family and life cycle occurred in the five orchards were summarized as 36 family and 105 species in apple orchard, 34 family and 126 species in grape orchard, 34 family 126 species in peach orchard, 33 family 98 species in pear orchards, and 36 family 111 species in plum orchard. In addition to life cycle of weeds, most orchards except for pear orchard were dominant to biennial weeds. The most dominant importance value was observed in pear orchards as 6.57%. In winter's season, the weeds were summarized as 31 family and 89 species in apple orchard, 28 family and 71 species in grape orchard, 32 family 111 species in peach orchard, 27 family 68 species in pear orchards, and 33 family 83 species in plum orchard. In summer's season, the weeds were distributed as 31 family and 101 species in apple orchard, 27 family and 69 species in grape orchard, 29 family 91 species in peach orchard, 31 family 94 species in pear orchard, and 31 family 97 species in plum orchard. In winter season, the most dominant weeds in apple, grape, peach, pear, and plum orchard were Capsella bursa-pastoris, Laria media, Capsella bursa-pastoris, Capsella bursa-pastoris, and Erigeron canadensis, in turn. In summer season, the most dominant weeds in apple, grape, peach, pear, and plum orchard were Acalypha australis, Acalypha australis, Setaria viridis, Setaria viridis, and Setaria viridis, respectively.

Effect of Plum Wine on the Lipid Metabolism and Lipid Peroxidation of Rats (자두와인의 섭취가 흰쥐의 지질대사 및 지질과산화에 미치는 영향)

  • Yoon, Ok-Hyun;Kang, Byung-Tae;Lee, Jae-Woo;Kim, Kwang-Ok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.4
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    • pp.422-427
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    • 2008
  • The effect of plum wine on lipid metabolism and lipid peroxidation in rats with chronic ethanol consumption was evaluated. Sprague-Dawley rats were randomly divided into the following 4 groups; water (NC), alcohol control (AC), low concentration plum wine (LP) and plum wine (P). The alcohol content of experimental drinking water of AC, LP and P were 6%, 6% and 12%, respectively. Animals were fed AIN-76 diet and experimental drinking water for 4 weeks. LP group showed significantly decreased liver weight per 100 g body weight, the levels of total cholesterol and atherogenic index in plasma whereas the ratio of HDL-cholesterol to total cholesterol was significantly increased in comparison to that of AC group. LP and P groups showed significantly decreased total lipid, total cholesterol in liver tissue, AST and ALT activities of plasma as compared with that of AC group. LP and P groups showed a significant decrease in the level of plasma lipid peroxidation products and LP group showed a significant decrease in the level of liver lipid peroxidation products as compared with that of AC group. These results suggest that supplementation of low concentration plum wine may exert more beneficial effects than pure alcohol beverage on lipid metabolism and lipid peroxidation products in chronically alcohol-treated animals by improving lipid profiles in plasma and liver tissues and decreasing plasma and hepatic lipid peroxidation product.

Development and Study of Separator for Plum and Pulp (매실 씨 및 과육 분리기 개발 및 연구)

  • Park, Woo-Jun;Yang, Kyu-won;Kim, Hyuck-Joo;Lee, Sang-Yoon;Jung, Bo-RA;Kim, Jung-Sil
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.2
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    • pp.378-385
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    • 2021
  • The production of plum jangachi requires the cleaning of plums, immersion in saltwater, separation of seed and pulp, removal of impurities, and addition of sugar. In most farms, the separation of plum seed and pulp is carried out manually, requiring considerable labor, which is why plum jangachi is expensive. To solve this problem, this study designed and manufactured automatic, semi-automatic plum seed and pulp separators. During the design process, the characteristics were compared, and the machine power was determined through on-site test after manufacture. As a result, automatic machines used plums 180° arrayed and six reverse-edged blades, semi-automated plums 180° arrayed, and six blades, each with a 68% and 57% pulp recovery rate and a machine power of 80 kg/h and 62 kg/h respectively. Overall, the mechanization of plum processed food will reduce labor and increase the market value of plums compared to the previous method.

Effect of ethphon treatment on the maturation of Plum fruits(Prunus salisina) and changes of the quality as affected by storage conditions (에테폰 처리가 자두과실의 성숙과 수확후 유통방법이 품질에 미치는 영향)

  • 임병선;이종석
    • Food Science and Preservation
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    • v.3 no.2
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    • pp.155-170
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    • 1996
  • In order to determine the physiolosical characteristics of plum fruits(Oishi wase) during maturation and guilty according to temperature after harvest, (2-chloroethyl) phosphonic acid(ethphon) 390ppm was sprayed on plum tree 11days before commercial matuarity in Suwon area. And also this study was acted to investigate fruits quility(Formosa) influenced by temperature(room, low) and polyethylene films(0.03, 0.06, 0.1mm), 1. Effect of ethphon on the fruits maturation and fruits(Oishi wase) quility according to temperature ofter harvest. Ethephon stimulated fruits ripening but the firmness was reduced rapidly. Soluble solids and titratable acidity was not very different than each treatment. Carbon dioxide and ethylene production were advanced and the production peak were shown earlier by ethephon treatment as compared with control fruit. Anthocyanin development was enhansed rapidly by ethephon treatment but it exerted a bad influence on fruits color after harvest, The soluble sugars in fluits were mainly glucose, sucrose, and fructose. Those content were higher in treated fruit than control. The organic acid was mainly malic acid. The shelf life was less than about 5days at room temperature and about 10 days at low temperature. 2. Fruits(Formosa) quility as affected by polyethylene film bagging. The polyethylene films well maintained the firmness both room and low temperature. Low temperature was more effective in maintaining titratable acidity than room temperature, especially polyethylene films. On the Other hand, soluble solids content was not shown wide differance between room and low temperature. Polyethylene film showed a high resperation rate, the rate was higher at room temperature than low temperature and thicker films revealed higher rate. Otherwise, ethylene production was low in all treatment Polyethylene film inhibited the coloration of fruits, decreased anthocyanin content. Fruits coloration delayed by low temperature in control. The shelf life of plum fruits was about 6 days at room temperature and 13 days at low temperature in control Polyethylene film had no advantage on shelf life both at room and low temperature.

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Experimental Studies on the Expression of Hair Growth Related Factors after Acupuncture & Moxibustion Therapy (침구요법(鍼灸療法)에 의한 발모관련 인자들의 발현에 대한 실험적 연구)

  • Kim, Ho-Il;Kim, Cheong-Moo;Lee, Chang-Hyun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.4
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    • pp.674-682
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    • 2011
  • The present study was undertaken to investigate the effect of acupuncture & moxibustion therapy on the hair follicle growth of skin 5 days and 10 days by macroscopic, microscopic and immunohistochemical methods. The results were as follows : Macroscopic hair follicle growth of plum-blossom needle treated group and strong moxibustion treated group was more increase than that of control group. Microscopic hair follicle growth of plum-blossom needle treated group and strong moxibustion treated group was hair growing cycle, anagen phase VI and that of control group and weak moxibustion treated group was hair growing cycle, anagen phase IV. Immunohistochemical observations on the expression of various growth factors, enzyme and receptor in hair follicle cycle after local treatment of acupuncture & moxibustion therapy are as follows: Expression of fibroblast growth factor was more intense in epidermis in plum-blossom needle treated group, epidermis and secondary hair germ cells in strong moxibustion treated group than control group. Expression of epidermal growth factor was more intense in epidermis in all experimental groups, and secondary hair germ cells in moxibustion treated group than control group. Expression of c-kit receptor was more intense in epidermis, secondary hair germ cells, outer root sheath in all experimental groups than control group. Expression of protein kinase C-${\alpha}$ was more intense in epidermis, secondary hair germ cells, outer root sheath in all experimental groups than control group. Expression of vascular endothelial growth factor was more intense in epidermis, bulge, secondary hair germ cells, outer root sheath in plum-blossom needle treated group and strong moxibustion treated group than control group. We concluded that acupuncture & moxibustion therapy related to the expression of various growth factors, enzymes and receptor on the hair growth cycle for hair growth.

Biphasic augmentation of alpha-adrenergic contraction by plumbagin in rat systemic arteries

  • Kim, Hae Jin;Yoo, Hae Young;Zhang, Yin Hua;Kim, Woo Kyung;Kim, Sung Joon
    • The Korean Journal of Physiology and Pharmacology
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    • v.21 no.6
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    • pp.687-694
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    • 2017
  • Plumbagin, a hydroxy 1,4-naphthoquinone compound from plant metabolites, exhibits anticancer, antibacterial, and antifungal activities via modulating various signaling molecules. However, its effects on vascular functions are rarely studied except in pulmonary and coronary arteries where NADPH oxidase (NOX) inhibition was suggested as a mechanism. Here we investigate the effects of plumbagin on the contractility of skeletal artery (deep femoral artery, DFA), mesenteric artery (MA) and renal artery (RA) in rats. Although plumbagin alone had no effect on the isometric tone of DFA, $1{\mu}M$ phenylephrine (PhE)-induced partial contraction was largely augmented by plumbagin (${\Delta}T_{Plum}$, 125% of 80 mM KCl-induced contraction at $1{\mu}M$). With relatively higher concentrations (>$5{\mu}M$), plumbagin induced a transient contraction followed by tonic relaxation of DFA. Similar biphasic augmentation of the PhE-induced contraction was observed in MA and RA. VAS2870 and GKT137831, specific NOX4 inhibitors, neither mimicked nor inhibited ${\Delta}T_{Plum}$ in DFA. Also, pretreatment with tiron or catalase did not affect ${\Delta}T_{Plum}$ of DFA. Under the inhibition of PhE-contraction with L-type $Ca^{2+}$ channel blocker (nifedipine, $1{\mu}M$), plumbagin still induced tonic contraction, suggesting $Ca^{2+}$-sensitization mechanism of smooth muscle. Although ${\Delta}T_{Plum}$ was consistently observed under pretreatment with Rho A-kinase inhibitor (Y27632, $1{\mu}M$), a PKC inhibitor (GF 109203X, $10{\mu}M$) largely suppressed ${\Delta}T_{Plum}$. Taken together, it is suggested that plumbagin facilitates the PKC activation in the presence of vasoactive agonists in skeletal arteries. The biphasic contractile effects on the systemic arteries should be considered in the pharmacological studies of plumbagin and 1,4-naphthoquinones.

Occurrence of Powdery Mildew of Japanese Plum Caused by Podosphaera tridactyla in Korea (Podosphaera tridactyla에 의한 자두나무 흰가루병 발생)

  • Lee, Seong-Chan;Han, Kyung-Sook;Cho, Sung-Eun;Park, Ji-Hyun;Shin, Hyeon-Dong
    • Research in Plant Disease
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    • v.18 no.1
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    • pp.49-53
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    • 2012
  • Occurrence of powdery mildew was found on Japanese plum (Prunus salicina) growing in private gardens in Yanggu, Hongcheon, Hoengseong, and Gapyeong of central Korea, in 2011. Despite of extensive survey, however, there was no powdery mildew infection in commercial orchards of Japanese plum in Korea. The initial symptoms included white, evanescent mycelia and irregular patches on leaves and young stems. Infected leaves later showed partial distortion and diffuse red-purple discoloration. Based on morphological characteristics of holomorph and phylogenetic analysis, the causal fungus was identified as Podosphaera tridactyla. This is the first report of powdery mildew on Japanese plum in Korea.

Physicochemical Properties of Salt-fermented Mytilus edulis Added with Various Seasoning Sauces (진주담치 양념젓갈의 이화학적 특성)

  • Park, Jung-Suk
    • Food Science and Preservation
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    • v.18 no.3
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    • pp.335-340
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    • 2011
  • Three kinds of salt-fermented Mytilus Edulis were produced: a Mytilus edulis seasoning sauce salt-fermented with Lactobacillus Plantarum CHO55, a Mytilus edulis seasoning sauce salt-fermented with Lactobacillus Plantarum CHO55 with teriyaki sauce added; and a A1ytilus edulis seasoning sauce salt-fermented with Lactobacillus Plantarum CHO55 with plum added. The salt-fermented Mytilus edulis with teriyaki sauce was high in crude protein (11.44%), and the plum seasoning sauce was more or less high in crude fat. Among the amino acids of the ingredients, glutamic acid was the most abundant in all the samples. The sauce with the highest total amino acid content of 9,169.48 mg per 100 g was the salt-fermented Mytilus edulis that was fermented with Lactobacillus Plantarum CHO55. Among the major fatty acids, C16:0 (palmitic acid), which is the main constituent of saturated fatty acid, was the most abundant; and the salt-fermented Mytilus edulis seasoning sauce that was fermented with Lactobacillus Plantarum CHO55 with plum added had a slight high amount of C18:2 (linoleic acid). The sensory evaluation showed the following sauces receiving the highest scores in all the measurement items, in this order, though the difference in their scores was not statistically significant: the salt-fermented seasoning sauce with plum added and the salt-fermented seasoning sauce with teriyaki sauce added.