• Clinical and Experimental Reproductive Medicine
• /
• v.24 no.1
• /
• pp.43-49
• /
• 1997

The objective of this study is to determine developmental pattern and cell allocation to the ICM and TE in haploid and diploid of embryos following parthenogenetic activation and in vitro fertilization. The incidence of development to blastocyst was lower in the combined treatments of ethanol stimulation and cytochalasin B as compared to the control. However, the combined ethanol stimulation and cytochalasin B treatment (diploid) accelerated development to the blastocyst as compared to the ethanol treatment alone (haploid). Significantly reduction in the average number of total cells and ICM was observed in the parthenotes alone as compared to fetilized embryos, but those of combined ethanol stimulation and cytochalasin B treatment embryos were significantly increased as compared to ethanol alone embryos. These results suggested that the ploidy affects preimplantation developmental patters and cell allocation to the ICM and TE in the porcine.

• Korean Journal of Plant Resources
• /
• v.19 no.2
• /
• pp.360-364
• /
• 2006

The gametophytes of Cyrtomium falcatum and Cyrtomium caryoptideum var. coreanum arising from spores were mechanically homogenized and cultured In vitro, to study their gametophyte ontogeny and sporophyte development. Homogenized gametophytic tissues formed as one-dimensional filaments after 2 weeks in culture and then glowed into blanched gamatophytes after 4 weeks. After 6 weeks, which were developed to two dimensional plates with apical notch and meristem in central zone. After 8 weeks in culture, apomictic buds were formed on the midribs without archegonium formation and these buds developed to sporophytes after 10 weeks in culture. Flow cytometric analysis of gametophytes and apomictic sporophytes revealed that both forms had the same ploidy level in C. falcatum and C. caryoptideum vu. coreanum, respectively. This is to certify that C. caryoptideum var. coreanum was an apomictic fern as well as C. falcatum.

• Radiation Oncology Journal
• /
• v.7 no.1
• /
• pp.1-13
• /
• 1989

Effects of ionizing radiation alone and combined with chemotherapy on tumor growth and it's clonal specificity monitored by changes in distribution of chromosome number were studies in A549 ceil line originated from human adenocarcinoma of the lung. Radiation (300 rad, 600 rad and 900 rad) were delivered with or without 5-FU. Forty eight hours later, 57.5% of growth inhibition of cell w8s seen in cells treated with 5-FU concentration of $0.4{\mu}g/ml$ for 24hr exposure. Cell survival curves after radiation with and without 5-FU were made. Chromosomal analysis of cells in metaphase in control, and in cells treated with 300 rad of radiation, or $0.4{\mu}g/ml$ of 5-FU treatment, and combined treatment of both were done to examine the changes in ploidy and number of chromosome. Radiation combined with S-FU enhanced growth inhibition of A549 cells. However, no evidence of synergegic effects in growth. inhibition was observed in the cells treated with the combination therapy. Pattern of chromosomal distribution of survived cells were shifted from hyperploidy to hypoploidy by single dose of radiation (300 rad). As radiation dose increased a large number of hypoploidy cells were observed. Following treatment of cells with 5-FU, chomosomal distribution of survived cells were also shifted to hypodiploidy which were seen in cells treated with radiation, The ceil treated with 5-FU and fellowed by radiation within 24 hrs had cell with increased number of hypodiploidy cells. Almost same type of chromosomal changes were reproduced in cells treated with combined treatment with radiation and 5-FU. Minor differences were that cells with fewer number of chromosome were more frequent in cells treated with combined therapy. Further increase in cells of hypoploidy (93%) having 1-10 chromosome were induced by additional radiation. Therefore, the enhanced therapeutic effect of 5-FU combined with radiation of A549 cells appeared to be additive rather than synergistic.

• Journal of the Korean Society of Costume
• /
• v.62 no.6
• /
• pp.200-217
• /
• 2012

The purpose of this research is to examine the specificity of grids to define the characteristics of clothes styles in the Joseon Dynasty period. The significance of examining of the specificity of grids is to find out arbitrary types of the features of grids involved in structuring the Jeogori in the Joseon Dynasty period one by one. The Visual Linguistic Theory was introduced as a methodological tool to exquisitely analyze the characteristics of grids in deep structures of Jeogori in the Joseon Dynasty period. This theory strives to examine sample distribution, the distribution of samples by quality and the distribution of the types of ploidy features. Through the examination, the results are as follows. The grid systems of the Jeogori consisted of diverse proportion systems reaching 86 cases, that is, sequence systems composed of multi-functional, multi-combined bodies. Most ornamental grids had feature angles distributed in a range of $2-20^{\circ}$ that showed a common preference for low sloped diagonal lines or small curvature. Although the preference for certain feature angles were prominent, the feature angles that were used were generally distributed evenly among diverse feature angles to show the characteristics of separation. Therefore, Jeogori makers in the Joseon Dynasty period can be considered as having experimented with many proportion systems to show their aesthetics. In conclusion, based on the results of the examination of feature distributions and related methods to allocate ploidy features, O-type accounted for 66% and thus it was identified that the Jeogori was characterized by O-type. Therefore, it was identified that the characteristic of the Jeogori in the Joseon Dynasty period consisted of O-type fractal structures which are formative structures unique to our nation.

• Current Research on Agriculture and Life Sciences
• /
• v.32 no.2
• /
• pp.99-103
• /
• 2014

This study investigated the effects of cryopreserving Chrysanthemum morifolium cv. 'White ND' shoot tips for eliminating viroids. As a result, smaller shoot tips (2-3 LP, 1mm) showed a better survival and regrowth than larger shoot tips (4-5 LP, 1.5mm). The most effective vitrification solution for survival and regrowth was PVS3, which induced a high survival rate after 60 minutes of incubation. For a high efficiency, the best pre-treatment condition for vitrification was incubation in 88 mM sucrose for 24 h, 0.3M sucrose for 16 h, 0.5 M sucrose for 6 h, and 0.7 M sucrose for 3 h, in a descending order. The ploidy levels were the same in the mother plants and following cryopreservation, which confirmed the absence of any gene mutation.

• Korean Journal of Plant Resources
• /
• v.29 no.3
• /
• pp.331-338
• /
• 2016

The present study aimed to compare and investigate the morphological characteristics and yield components according to ploidy level of diploid and tetraploid Platycodon grandiflorum under vinyl-greenhouse and open field conditions. Plant height of diploid and tetraploid P. grandiflorum was 51.3 ㎝, 54.0 ㎝, respectively. The results revealed that the plants grown in the vinyl-greenhouse showed significantly higher growth compared to those grown in the open field. Regardless of the growing place, diploid and tetraploid of P. grandiflorum showed the rapid elongation of internodes after 4 and 3 internodes respectively and elongation tends to be decreased as entering the flower-bud differentiation period. The starting day of flowering in vinyl-greenhouse cultivation was found to be faster than that of the open field cultivation by 2∼3 days and tended to be delayed by about 5∼6 days in tetraploid P. grandiflorum compared to diploid. Fresh weight of roots from the vinyl-greenhouse cultivation showed a high quantity as 34.2g and 49.4g in diploid and tetraploid P. grandiflorum, respectively and especially tetraploid P. grandiflorum was found to be increased by approximately 44.4% compared to other plots.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.23 no.2
• /
• pp.503-524
• /
• 1996

A series of 31 patients with primary oral squamous cell carcinoma (SCC) who were treated at Chonbuk National University Hospital during the years 1991-1995, were evaluated by dual parameter analysis in flow cytometric DNA measurement, Bryne's malignancy grading system, and the TNM classification. The aims of the present study were to discover that previously undetected aneuploid clones could be detected by dual parameter analysis and to determine the prognostic value of the above parameters. 1. Using dual parameter analysis of cytokeratin and DNA on disintegrated paraffin-embedded samples, aneuploid clones which were undetected by regular single parameter DNA analysis could be found among the cytokeratin-selected cells. DNA aneuploidy from paraffin-embedded samples were 15 cases compared with 10 cases using conventional DNA analysis. 2. The portion of aneuploid tumors showed slightly higher clinical stage and tumor size than the portion of diploid tumors, but the difference was not significant. The portion of DNA aneuploid tumors showed significantly higher mean mitosis and total malignancy scores than the portion of DNA diploid tumors. 3. The majority of the patients presented with clinical stage III and IV lesions showed significantly higher mean total malignancy score as compared to those with clinical stage I and II. 4. Histopathologic mean total malignancy score of the 31 cases was 12.7. Among the histologic parameters, mean mitosis score was correlated to the status of DNA ploidy and total malignancy score were correlated to the DNA ploidy and clinical staging.

• Korean Journal of Plant Resources
• /
• v.36 no.6
• /
• pp.588-596
• /
• 2023

Long-term culture of cell lines is an important issue in in vitro culture and in plant science. In this study, the regeneration ability and ex vitro acclimatization of regenerants were evaluated. The ploidy level of regenerants derived from long-term cultured cell lines was measured in Imperata cylindrica 'Rubra', Poaceae. Adventitious buds (shoots) were successfully induced from five-year-cultured calli on MS medium containing 0.1 mg/L BA or 2.0 mg/L TDZ, combined with 0.01 mg/L auxins (IAA, IBA, NAA and 2,4-D), respectively. Adventitious roots were also induced on MS medium containing 0.01 mg/L auxins (IBA, NAA and 2,4-D), respectively. Interestingly, regenerants with both red and green leaf were successfully obtained when regenerants were cultured on MS medium with 9% sucrose. Regenerants derived from long-term cultured calli were transferred to pots using an optimal acclimatization process and successfully adapted to both pot and soil conditions. Moreover, the ploidy level was measured using calli and regenerants that had been kept on MS medium containing various kinds of plant growth regulators (PGRs).

• Korean Journal of Breeding Science
• /
• v.41 no.3
• /
• pp.213-219
• /
• 2009

This study was carried out to establish breeding system for interspecific hybridization of Lilium using spontaneous meiotic polyploidization of $F_1$ OA hybrid and its progenies. Pollen viability and germination of $F_1$ OA interspecific hybrid showed variation by collection time, indicating that pollen grain in June, which was natural blooming season, had better viability and germination ability than in October. 2n-gametes from spontaneous meiotic polyploidization of OA hybrid were crossed with either diploid Asiatic or Oriental hybrid, and subsequent progenies crossed with Asiatic hybrid as female and OA hybrid as male showed the highest efficiency, where 2x-2x A$\times$OA produced 0.64 plants per cross combination, while 2x-2x, O$\times$OA and 2x-2x, OA$\times$A crosses produced 0.42 and 0.4 plant per ovary, and especially no progeny was obtained in 2x-2x, OA$\times$O cross combination. For the subsequent progeny production, a cross between diploid Asiatic hybrid as female and diploid OA hybrid as male was the best ploidy combination. Ploidy level of subsequent progeny derived from backcross with 2n-gametes of OA hybrid revealed that triploids from 2x-2x OA$\times$A, tetraploids from both 4x-2x, AA$\times$OA and OO$\times$OA, diploid from 2x-2x, O$\times$OA, and diploids or triploids from 2x-2x, A$\times$OA crosses were produced respectively.

• The Korean Journal of Malacology
• /
• v.16 no.1_2
• /
• pp.11-16
• /
• 2000

The electrophoretic banding patterns of 6-phosphogluconate dehydrogenase (PGD) in the two different chromosomal ploidy groups of Gyraulus were compared. The monomeric or dimeric banding patterns or allelic variations in a locus of PGD were observed in four diploid populations (Osan, Sohre, Kimpo and Kangwha) of G. convexiusculus occurring in Korea, whereas the isozyme banding patterns encoded by at least 3 different loci were shown in the tetraploid populations of G. (Torquis) groups collected from Michigan, the U.S.A. Of 3 different tetraploid groups, G. (T.) circumstriatus group showed 3 monomorphic isozyme banding patterns, and the other 2 populations showed some allelic variations. Such results provided good evidence to differentiate tetraploid subgenus Torquis group from the diploid Gyraulus populations.