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Fertility Restoration of F1 OA Interspecific Hybrid by Spontaneous Meiotic Polyploidization and Analysis of Their Progenies  

Chung, Mi-Young (Department of Horticulture, Kyungpook National University)
Chung, Jae-Dong (Department of Horticulture, Kyungpook National University)
Van Tuyl, Jaap M. (BU Biodiversity and Breeding, Plant Research International, Wageningen University and Research Centre)
Lim, Ki-Byung (Department of Horticulture, Kyungpook National University)
Publication Information
Korean Journal of Breeding Science / v.41, no.3, 2009 , pp. 213-219 More about this Journal
Abstract
This study was carried out to establish breeding system for interspecific hybridization of Lilium using spontaneous meiotic polyploidization of $F_1$ OA hybrid and its progenies. Pollen viability and germination of $F_1$ OA interspecific hybrid showed variation by collection time, indicating that pollen grain in June, which was natural blooming season, had better viability and germination ability than in October. 2n-gametes from spontaneous meiotic polyploidization of OA hybrid were crossed with either diploid Asiatic or Oriental hybrid, and subsequent progenies crossed with Asiatic hybrid as female and OA hybrid as male showed the highest efficiency, where 2x-2x A$\times$OA produced 0.64 plants per cross combination, while 2x-2x, O$\times$OA and 2x-2x, OA$\times$A crosses produced 0.42 and 0.4 plant per ovary, and especially no progeny was obtained in 2x-2x, OA$\times$O cross combination. For the subsequent progeny production, a cross between diploid Asiatic hybrid as female and diploid OA hybrid as male was the best ploidy combination. Ploidy level of subsequent progeny derived from backcross with 2n-gametes of OA hybrid revealed that triploids from 2x-2x OA$\times$A, tetraploids from both 4x-2x, AA$\times$OA and OO$\times$OA, diploid from 2x-2x, O$\times$OA, and diploids or triploids from 2x-2x, A$\times$OA crosses were produced respectively.
Keywords
2n-gametes; Pollen viability; Ploidy level; $F_1$ sterility; OA hybrid;
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