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Prothallus Morphogenesis of Cyrtomium falcatum (L.) Presl and Cyrtomium caryotideum var. coreanum Nakai In vitro Culture  

Jeong Jin-A (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
Lee Cheol-Hee (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
Publication Information
Korean Journal of Plant Resources / v.19, no.2, 2006 , pp. 360-364 More about this Journal
Abstract
The gametophytes of Cyrtomium falcatum and Cyrtomium caryoptideum var. coreanum arising from spores were mechanically homogenized and cultured In vitro, to study their gametophyte ontogeny and sporophyte development. Homogenized gametophytic tissues formed as one-dimensional filaments after 2 weeks in culture and then glowed into blanched gamatophytes after 4 weeks. After 6 weeks, which were developed to two dimensional plates with apical notch and meristem in central zone. After 8 weeks in culture, apomictic buds were formed on the midribs without archegonium formation and these buds developed to sporophytes after 10 weeks in culture. Flow cytometric analysis of gametophytes and apomictic sporophytes revealed that both forms had the same ploidy level in C. falcatum and C. caryoptideum vu. coreanum, respectively. This is to certify that C. caryoptideum var. coreanum was an apomictic fern as well as C. falcatum.
Keywords
Gametophyte ontogeny; Homogenized; Apomictic fern;
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