• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.239.2-239
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• 1997

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• Mycobiology
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• 제38권4호
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• pp.295-301
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• 2010

We evaluated the antioxidant activity and tyrosinase inhibitory effects of Pleurotus ostreatus fruiting bodies extracted with acetone, methanol, and hot water. The antioxidant activities were tested against $\beta$-carotene-linoleic acid, reducing power, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity, and ferrous chelating ability. Furthermore, phenolic acid and flavonoid contents were also analyzed. The methanol extract showed the strongest $\beta$-carotene-linoleic acid inhibition as compared to the other exracts. The acetone extract (8 mg/mL) showed a significantly high reducing power of 1.54 than the other extracts. The acetone extract was more effective than other extracts for scavenging on 1,1-diphenyl-2-picrylhydrazyl radicals. The strongest chelating effect (85.66%) was obtained from the acetone extract at 1.0 mg/mL. The antioxidant activities of the extracts from the P. ostreatus fruiting bodies increased with increasing concentration. A high performance liquid chromatography analysis detected seven phenolic compounds, including gallic acid, protocatechuic acid, chlorogenic acid, naringenin, hesperetin, formononetin, and biochanin-A in an acetonitrile and 0.1 N hydrochloric acid (5 : 1) solvent extract. The total phenolic compound concentration was $188{\mu}g$/g. Tyrosinase inhibition of the acetone, methanol, and hot water P. ostreatus extracts increased with increasing concentration. The results revealed that the methanol extract had good tyrosinase inhibitory ability, whereas the acetone and hot water extracts showed moderate activity at the concentrations tested. The results suggested that P. ostreatus may have potential as a natural antioxidant.

• 한국균학회지
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• 제24권4호통권79호
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• pp.322-328
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• 1996

왕호장근(Reynoutria sachalinensis)은 국내에서 자라는 마디풀과에 속하는 다년생 잡초로써 단위 면적당 Biomass가 매우 높으므로 버섯재배의 첨가 기질로써 이용가능성을 시험하였다. 대조구인 malt extract agar보다 왕호장근 1/4 희석 배지에서 느타리 균사 생장이 우수하였으며 1/8 희석 배지에서는 대조구보다 3배나 균사생장이 빨랐으며 균총의 밀도도 매우 높았다. 또한 느타리 균사가 왕호장근 한천배양기의 최적 산도 및 희석비율을 알아본 결과 pH 6.5, 희석비율 1/8이 느타리 균사생장에 가장 적합하였다. 왕호장근, 톱밥 및 미강를 각각 혼합하여 균사생장을 비교하여 본 결과 왕호장근을 넣은 배지에서 느타리 생강이 우수하게 나타났으며 1.5 내지 2배의 균사생장을 보였다. 왕호장근 한천배양기의 희석비율 l/4, 1/8와 pH 4.5, 6.5, 8.5에서 느타리 균사와 Trichoderma sp.의 대칭배양에서 산도 및 희석농도와 관계없이 느타리 균사생장이 월등하게 나타났으며 Trichoderma sp.의 균사생장을 억제하였다. 왕호장근, 톱밥, 미강을 혼합하여 종균 제조시 톱밥+왕호장근 10%+미강 10%의 배지에서 균사활착이 대조구보다는 4내지 5일 빨랐으며 2배 이상의 균사생장을 보였고 균사의 밀도도 가장 우수하였다.

• 한국버섯학회지
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• 제20권2호
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• pp.43-49
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• 2022

Pleurotus ostreatus is a globally cultivated mushroom crop. Cap color is a quality factor in P. ostreatus. However, cap color can spontaneously mutate, degrading the quality of the mushroom on the market. Early detection and removal of mutant strains is the best way to maintain the commercial value of the crop. To detect the cap color mutant Gonji7ho, molecular markers were developed based on insertion/deletions (InDels) derived from the comparison of mitogenomes of Gonji7ho and Gonji7hoM mushrooms. Sequencing, assembly, and comparative analysis of the two mitogenomes revealed genome sizes of 73,212 bp and 72,576 bp with 61 and 57 genes or open reading frames (ORFs) in P. ostreatus Gonji7ho and Gonji7hoM, respectively. Fourteen core protein-encoding genes, two rRNA, and 24 tRNA with some OFRs were predicted. Of the 61 genes or OFRs in the wild type, dpo, rpo, and two orf139 were missing (or remnant) in the mutant strain. Molecular markers were developed based on the sequence variations (InDels) between the two mitogenomes. Six polymorphic molecular markers could detect the mutated mitochondria by PCR. These results provide basic knowledge of the mitogenomes of wild-type and mutant P. ostreatus, and can be applied to discriminate mutated mitochondria.

• Mycobiology
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• 제36권4호
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• pp.228-232
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• 2008

Mushroom cultivation has been started recently in Bangladesh. Awareness of the nutritional and medicinal importance of mushrooms is not extensive. In this study, the nutritional values of dietary mushrooms- Pleurotus ostreatus, Pleurotus sajorcaju, Pleurotus florida and Calocybe indica that are very popular among the cultivated mushrooms in Bangladesh have been determined. These mushrooms were rich in proteins ($20{\sim}25%$) and fibers ($13{\sim}24%$ in dry samples) and contained a lower amount of lipid (4 to 5%). The carbohydrate contents ranged from 37 to 48% (on the basis of dry weight). These were also rich in mineral contents (total ash content is $8{\sim}13%$). The pileus and gills were protein and lipid rich and stripe was carbohydrate and fiber-rich. The moisture content of mushrooms ranged from 86 to 87.5%. Data of this study suggest that mushrooms are rich in nutritional value.

• 한국버섯학회지
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• 제20권4호
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• pp.193-198
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• 2022

The biological efficiencies of promising Pleurotus spp. were evaluated. Pleurotus ostreatus, Pleurotus tuberregium,and Pleurotus sajor-cajuwere investigated at temperatures of 22℃, 20℃, and 18℃ to determine their ability to adapt to temperatures that are likely to be found in subtropical regions. The experiment was conducted using a substrate comprising Popular sawdust 60% + cotton seed 20% + beet pulp 20% under a relative humidity of 65%. The results indicated that there were significant differences in terms of biological efficiency between the species: P. tuber regium 128.84% at 22℃, P. ostreatus 108.41% at 22℃, and P. sajor-caju is 80% at 20℃. The common temperatures at which all species showed the highest biological efficiencies were 22℃ and 20℃. Therefore, the production of the evaluated species was equal to or surpassed biological efficiencies reported in tropical environments, thereby demonstrating a potential opportunity to adapt to tropical environments without compromising quality standards.

• 한국식품영양학회지
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• 제33권1호
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• pp.64-73
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• 2020

The purpose of this study was to investigate the antioxidant activity and β-glucan content of extracts extracted by varying the temperature at 30, 55 and 80℃ after hot air drying or freeze drying of Pleurotus ostreatus and Pleurotus eryngii. For the analysis antioxidant activity of each mushroom, β-glucan, total phenol, flavonoid contents, and DPPH·ABTS⁺·Nitrite assay were measured. Also, the β-glucan content, total flavonoid content and ABTS⁺ scavenging activity increased with freeze drying rather than hot air drying, and increased with increasing extraction temperature in both mushrooms. However, the total phenol and nitrite scavenging activity increased with hot air drying rather than freeze drying, and decreased with increasing temperature in both mushrooms. DPPH scavenging activity was not significant in both mushrooms, but decreased with increasing extraction temperature. Pearson's correlations between total flavonoid content and antioxidant activities were r=0.719~0.753 (p<0.01). As a result, the β-glucan content, total flavonoid content, and ABTS radical cation scavenging activity were highest during freeze drying and extraction at 80℃. And the highest total phenol content, DPPH radical scavenging activity and nitrite scavenging activity were obtained during hot air drying and extraction at 30℃.

• 한국균학회지
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• 제11권1호
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• pp.1-7
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• 1983

진탕배양에서 느타리와 목이의 균사체(菌絲體) 생산(生産)에 대한 실험(實驗)의 결과(結果)를 요약(要約)하면 다음과 같다. 1. 균사체생산(菌絲體生産)에 양호(良好)한 탄소원(炭素源)은 P. ostreatus경우 glucose와 mannitol이었고 A. auricula-judae는 mannitol과 fructose이었다. 유기산(有機酸)중에서는 양균주(兩菌株) 모두 fumaric acid에서 제일 양호(良好)하였다. 2. 균사체생산(菌絲體生産)에 양호(良好)한 질소원(窒素源)은 P. ostreatus 경우 peptone, urea와 asparagin, DL-threonine이 었고 A. auricula-judae는 peptone, Casamino acid와 L-Serine, DL-threonine이었다. 3. 균사체생산(菌絲體生産)에 양호(良好)한 vitamin은 A. auricula-judae 경우 folic acid, thiamine이었고 A. auricula-judae는 folic acid, inositol, riboflavin이었다. 4.양균주(兩菌株)가 $MgSO_4$는 0.08%, $KH_2PO_4$ 0.2%에서 균사체생산(菌絲體生産)이 양호(良好)하였다. 5. 균사체생산(菌絲體生産)의 최적온도(最適溫度)와 pH는 P. ostreatus에서 $25{\sim}30^{\circ}C$, $pH\;5.5{\sim}6.0$이고 A. auricula-judae에서 $25{\sim}30^{\circ}C$, $pH6.0{\sim}7.0$이었다.

• 한국균학회지
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• 제23권3호통권74호
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• pp.197-201
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• 1995

느타리버섯 재배에 있어서 배지재료로 제지부산물의 사용 가능성을 검토한 결과 배지재료별 화학특성은 제지부산물이 폐면, 볏짚, 왕겨보다 CaO는 많았고, T-C, T-N, $P_2O_5$, MgO는 비슷하였으며 $K_2O$는 현저히 적었다. 균사생장은 혼합처리가 폐면단용보다 생장이 다소 빠르거나 비슷한 경향이었다. 초발이소요일수는 폐면단용보다 혼합처리가 비슷하거나 $1{\sim}2$일 정도 빠른 경향이었다. 배지종류별 수량은 폐면단용에 비해 폐면 10+왕겨 20+제지부산물 70% 처리를 제외하고는 증수되었으며, 특히 계면+제지부산물 30% 혼합처리가 21% 증수되어 가장 우수하였다. 경제성분석결과 폐면단용보다 폐면+제지부산물 30% 혼합처리에서 소득지수가 50% 높게 나타났다.

• The Plant Pathology Journal
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• 제22권1호
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• pp.68-74
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• 2006

A new virus with a dsRNA genome was isolated and characterized from the Suhan-:neutari strain (ASI 2504) of Pleurotus ostreatus, which was characterized as long and slightly bent with small caps on the stipe of fruit body. Thirty nm isometric viruses with three dsRNA segments (approximately 2.0, 1.84 and 1.82 kb in sizes) were isolated by ultracentrifugation in sucrose gradients. Western analysis of protein extracted purified viruses with anti-virus polyclonal antibody confirmed that viruses have two specific proteins (36 and 68 kDa). Computer analysis of 2.0 kb segment shows that high. sequence identity with RNA-dependent RNA polymerase (RdRp) of partitiviruses, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis, OMDV was most related to Pleurotus ostreatus virus 1.