• Journal of Aquaculture
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• v.22 no.1
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• pp.63-67
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• 2009

In order to establish optimum anesthesia concentration, we tested the efficacy of clove oil at five different concentrations in large sized (mean SL $17.1{\pm}2.21\;cm$) and small sized (mean SL $0.6{\pm}0.06\;cm$) dark-banded rockfish Sebastes inermis. Optimal anesthesia concentration for dark-banded rockfish was $150\;mgL^{-1}$ in both large and small sized fish. In general, fish exposed to higher anesthetic doses were rapidly induced but took longer to recover (P<0.05). Recovery time of small sized fish was longer than large sized fish in lower concentrations, while recovery time of large sized fish was longer than small sized fish in higher concentration (P<0.05). Using the established optimum aesthetic concentration, we evaluated the physiological response of dark-banded rockfish to clove oil by measuring plasma cortisol and glucose levels. Following administration of $150\;mgL^{-1}$ clove oil at $20^{\circ}C$ (optimum breeding temperature), plasma cortisol level was highest ($42.2{\pm}11.318{\mu}g/dL$) after 0 hour, while plasma glucose level was highest ($52.5{\pm}10.61\;mg/dL$) after 1 hour. Plasma cortisol and glucose concentrations required 6 and 2 hours, respectively, to return to pre-exposure levels.

• The Korea Journal of Herbology
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• v.30 no.2
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• pp.37-42
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• 2015

Objectives : The aim of this study was to investigate the effects of licorice supplementation on muscle injury, plasma cortisol, testosterone and insulin sensitivity after high intensity resistance exercise. Methods : The fourteen health college male students were voluntarily participated in this study and were randomly divided into 2 groups: Control group (CON, n=7), Licorice group (LR, n=7). LR group ingested 2 g/time of licorice extract (mixed with 100 ml of water) two times/day for 10 days while the CON group ingested 100 ml of water. All subjects performed a high intensity resistance exercise (half-squat, 8 RM at 80% one-repetition maximum, 5 sets, 1min rest). Blood samples were collect before (-7) and after (0) licorice supplementation, and then 1 day, 2 day and 3 day post exercise. After 10 day treatment, plasma creatine kinase, cortisol, testosterone, glucose, insulin were measured. To determine the insulin sensitivity, HOMA-IR was calculated. Results : Plasma creatine kinase activities were significantly elevated after exercise, but there was not different between two groups. The plasma cortisol and testosterone levels were not significantly different between two groups. Plasma glucose levels were increased at 1 day and 2 day after exercise in the LR comparing with CON group (P<0.05) but plasma insulin levels were significantly lower in comparison with CON. HOMA-IR were significantly lower in the LR than CON group at 0 day to 3 day (P<0.05). Conclusions : The results of the current study suggest that licorice supplementation for 10 days might not attenuate the high-intensity exercise-induce muscle injury but may enhance the whole-body insulin sensitivity.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.860-868
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• 2014

The study was performed to investigate changes in hematological responses and antioxidative enzyme activities (superoxide dismutase, SOD; catalase, CAT) of Japanese eel Anguilla japonica following exposure to 0 (control), 2.33, 4.60, 6,64 and 8.78 mM nitrite-N in fresh water for 48 h. Hematological parameters such as plasma nitrite, electrolytes, cortisol, glucose, glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), hemoglobin (Hb), methemoglobin (metHb) and NADH-methemoglobin reductase (NMR) were measured. Plasma nitrite, cortisol, metHb and NMR increased directly with increasing ambient nitrite concentration, while Hb content showed a progressive decline. Levels of plasma potassium, GOT and GPT of the eel exposed to 6.64 mM ambient nitrite were significantly higher than the control fish. The activity of SOD and CAT in plasma, gill and liver of the eel following exposure to nitrite were augmented by increasing ambient nitrite. Levels of plasma nitrite, metHb, NMR, cortisol, glucose and antioxidative enzyme activities of the eel exposed to 2.33 mM ambient nitrite were significantly higher than the control fish. This study suggested that the eel acutely exposed to elevated ambient nitrite causes nitrite-induced stress responses, changes in antioxidative enzyme activities and hematological parameters.

• The Journal of Internal Korean Medicine
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• v.11 no.1
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• pp.41-52
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• 1990

After intravenous administration of Se Pye San water extract in the rabbit, the change of plasma cortisol concentration, arterial blood $PCO_2$ and $PO_2$ was obtained such results as follows. 1. The plasma cortisol concentration in the control group was constant, but after intravenous administration of Se Pye San water extract at the dose of 0.2 ml/kg, the above concentration was increased significantly from 2 to 3 hours. Also, the above concentration was increased remarkably at the dose of 0.4 ml/kg from 1 to 4 hours. 2. After intravenous administration of Se Pye San water extract at each dose of 0.2 ml/kg and 0.4 ml/kg, arterial blood $PCO_2$ was decreased remarkably from 1 to 4 hours. 3. No change after intravenous administration of Se Pye San water extract at the dose of 0.2 ml/kg, while arterial blood $PO_2$ was decreased significantly at the dose of 0.4 ml/kg on 3 hours. As a results of the above, the therapeutic action of Se Pye San water extract effected with Jisu (止嗽), Jeong Cheon (定喘), Geo Dam, Cheong Yeol (淸熱) would be related with the increased both plasma cortisol concentration and arterial blood $PO_2$, and the decrease of arterial blood $PCO_2$.

• Anxiety and mood
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• v.4 no.1
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• pp.19-27
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• 2008

Objective : This study was conducted to evaluate the changes in the levels of neurotransmitters and cortisol in patients with chronic posttraumatic stress disorder (PTSD) and to evaluate their correlation with symptoms after long-term pharmacological treatment. Methods : Twenty-eight Vietnam veterans with chronic PTSD and 34 non-PTSD patients were consecutively recruited. The Combat Exposure Scale (CES), Mississippi Scale for Combat-Related Posttraumatic Stress Disorder (M-PTSD), Clinician Administered PTSD Scale (CAPS), Hamilton Rating Scale for Depression (HRSD) and Hamilton Anxiety Scale (HAS) were used to evaluate symptom severity. High performance liquid chromatography (HPLC) was used to measure the plasma levels of epinephrine, norepinephrine, and dopamine, and a radioimmunoassay (RIA) was performed to evaluate the plasma level of cortisol. Results : Plasma cortisol was significantly lower in PTSD patients than in control subjects, while there was no significant difference in plasma epinephrine, norepinephrine and dopamine between the two groups. The scores of M-PTSD, CAPS, HAMD and HAMA were signigicantly higher in PTSD patients than control group. Conclusion : After long-term treatment, the levels of neurotransmitters in PTSD patients returned to within the normal range, and the patients' symptoms showed some improvement. However, the core symptoms of PTSD continued to appear intermittently, and they are thought to be associated with hormonal systems, such as the HPA axis. It is also suggested that PTSD should be considered to be a complex disorder associated with multiple systems and that combinations of the effective medications for each system should be used to treat patients with PTSD.

• Reproductive and Developmental Biology
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• v.32 no.3
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• pp.199-203
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• 2008

This study was performed to analyze the characterization of plasma hormonal levels during pregnancy in the Hanwoo recipients pregnant by artificial insemination (AI) or somatic cell nuclear transfer (SCNT) embryos. The synchronized recipients pregnant by SCNT embryos produced by Hanwoo fetal fibroblast cells (n=8) and by AI (control, n=5) were used. The plasma hormonal levels were measured by RIA (P4 and E2) and ELISA (cortisol), respectively. In control, the increase of E2 and the decrease of P4 were occurred immediately before the initiation of parturition. The expression pattern of plasma P4 was similar in both groups from 50 to 10 days before parturition, however, it did not decrease even at the expected date of labor in the SCNT recipients. The plasma cortisol was expressed a lower level during pregnancy in the SCNT recipients. But, the cortisol was increased in the cow aborted around 100 days of pregnancy (n=1). Based on these results, it can be postulated that the failure of the hormonal changes immediately before parturition in the SCNT recipients may be one of the most important reasons for a delayed parturition in clone calving.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.819-826
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• 2013

The black seabream Acanthopagrus schlegelii is an important species for aquaculture in Korea. There are, however, no reports on the physiological responses to hypoxia in this species. The objective of this study was to evaluate the effects of acute hypoxia on the physiological stress responses (plasma cortisol as the primary response, and plasma glucose, hematocrit (Ht), hemoglobin (Hb), sodium, chloride, osmolality, AST, ALT and total protein as the secondary responses) of the fish. The experimental fish were exposed to 0.5 ppm dissolved oxygen (DO) in the seawater via two methods (progressive stepwise decline (Exp. I), and direct decline (Exp. II)). A highly significant increase was detected in plasma cortisol levels due to the hypoxia treatments, and fish in Exp. I had hormone levels that were significantly higher than the fish in Exp. II. For plasma glucose, there was no significant difference between the Exp. I fish and the control fish, whereas Exp. II fish showed significantly higher plasma glucose levels than Exp. I fish and control fish. Ht values increased in both hypoxia treatments; however, Hb concentrations increased only in Exp. I. Although plasma chloride levels were unaffected by acute hypoxia, plasma sodium and osmolality levels increased in Exp. I. Progressive hypoxia (Exp. I fish) increased plasma AST, ALT and total protein. These results suggest that the value and direction of changes to the investigated parameters can be used to determine the resistance of black seabream to acute hypoxia.

• Development and Reproduction
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• v.16 no.4
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• pp.279-287
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• 2012

We investigated the changes in plasma sex steroid hormones, testosterone (T), estradiol-$17{\beta}$ ($E_2$), 17,$20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$), 11-ketotestosterone (11KT) and cortisol levels from ribbed gunnel, Dictyosoma burgeri in associated with annual reproductive cycle. The gonadosomatic index (GSI) of females increased from November, peaked in February and decreased rapidly from March. The GSI of males also increased from November, peaked in January and then decreased gradually. In females, $E_2$ levels increased and remained high from December to February. The levels of T showed a similar tendency and correlated ($r_s$=0.898, p<0.01) with $E_2$ levels. The levels of $17{\alpha}20{\beta}P$ increased rapidly in February ($4.78{\pm}1.01ng/ml$) and peaked in July ($5.08{\pm}0.65ng/ml$). Cortisol level was peaked in March and correlated with $17{\alpha}20{\beta}P$ levels ($r_s$=0.696, p<0.01). In males, the levels of T was peaked in January and then decreased rapidly. The levels of 11KT were remained high from October to January. On the other hand, the levels of $17{\alpha}20{\beta}P$ fluctuated during reproductive cycle. These results suggest that plasma sex steroids in ribbed gunnels have annual periodicity, and that cortisol may involve in maturation of females.

• The Korean Journal of Pharmacology
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• v.28 no.1
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• pp.19-25
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• 1992

The effect of the nonbenzodiazepine anxiolytic, buspirone $(Buspar^R)$, a serotonin $(5-HT)_{1A}$ partial agonist, which also has dopamine $(DA)_2$ receptor antagonist properties, on prolactin and cortisol secretion was examined in eight normal male volunteers. The oral administration of buspirone (30 mg) significantly increased plasma prolactin concentrations but did not significantly increase plasma cortisol concentrations in this study. The oral administration of pindolol (30 mg), a beta adrenoceptor antagonist which is also a $5-HT_{1A}$ receptor antagonist, had no significant effect on basal prolactin or cortisol levels. Moreover, pretreatment with pindolol did not significantly inhibit the buspirone-induced increase in prolactin secretion. These preliminary data are suggestive that buspirone-induced prolactin secretion is not mediated via $5-HT_{1A}$ receptor activation.

• Journal of Korean Medical Ki-Gong Academy
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• v.1 no.1
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• pp.95-109
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• 1996

To investigate anti-stress effects of Manual Kigong, Manual-Apparatus Combined Kikong, and Systemic Kigong, this experiments were performed changing of plasma catecholaminess and serum cortisol measured. Also using D.I.T.I(Digital Infrared Thermongraphy Imaging), Pulse Analyzer, Electric Current on Neurometer, and Digital Flicker on Testing-Stressed. And following results were abtained. 1) Menual-Appartus Combined Kikong decreased significantly increasing concentration of plasma epinephrine and norepinephrine level in Testing-stressed. 2) Manual Kigong decreased increasing concentration of serum cortisol level in Testing-stressed. 3) Manual Kigong and Manual-Aparatues Combined Kikong increased decreasing thermomerty on the palm's center caused Testing-stressed. 4) All of three treats are small change in SET(Systolic Ejection Time) by Pulse Analyzer caused Testing-stressed. 5) More changes of Electric Current on the Neurometer Diagnosis caused Testing-stressed are as followed. Manual-Apparatus Combined Kikong >Systemic Kigong >Manual Kigong From the results, it may be concluded that Manual-Apparatus Combined Kikong Theraphy having more anti-stress effects than Manual Theraphy. Systemic Kigong Therapy.