• 한국작물학회지
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• 제47권6호
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• pp.443-447
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• 2002

참깨 재배조건에 따른 생육과 수량 및 품질 차이를 구명하고자 수행한 연구결과는 다음과 같다. 1. 비닐하우스 재배가 노지 재배에 비해 개화기가 빠른 반면 성숙기가 늦어 생식생장기간이 길었다. 또한 경장과 착삭부위장이 매우 길었다 한편 주당삭수가 많고 천립중이 무거워 수량이 57%가량 많았다. 2. 비닐하우스 재배에서는 6월 8일 파종구가 5월 9일 파종 구에 비해 착삭부위장이 길었다. 그리고 주당삭수가 많고 천립중이 무거웠다. 또한 착삭 상단부위 등숙율이 높고 착삭 중, 하단부위 천립중이 무거우나 수량은 비슷하였다. 3. 노지 재배에서는 5월 9일 파종구가 6월 8일 파종구에 비해 유효분지수와 주당삭수가 많았다. 그러나 착삭 상, 중단부위의 등숙율이 낮고 천립중이 가벼워 수량은 비슷하였다. 4. 종실의 $L^*$값은 재배조건에 따라 차이가 없었고 $a^*$값은 비닐하우스 재배에서 높았으나 $b^*$값은 노지 재배에서 높게 나타났다 그리고 파종기간 색차($\Delta E^*{ab}$)는 노지 재배에서 가장 높게 나타났다. 5. 비닐하우스 재배에서는 수용성 성분인 세사미놀 배당체 함량이 높고, 노지 재배에서는 세사민과 세사몰린 함량이 높게 나타났다.

• 한국작물학회지
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• 제7권1호
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• pp.161-165
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• 1969

Since Guha and Maheshwari's works on the induction of haploid plant from the cultured anther of Datura innoxia an her culture has become a big topic among geneticists and breeders. Presen paper is the summarized report on the anther culture method based on the author's recent researches.

• 생약학회지
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• 제23권4호
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• pp.264-267
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• 1992

The cytotoxic activity of medicinal plants was screened using A549 human lung cancer cell line. Plant materials were extracted with 80% methanol and fractionated to chloroform and water layers. Each methanol, chloroform, and water extract of thirty-two medicinal plants was tested for cytotoxic activity in A549 cell culture system and the cell viability was measured by SRB assay.

• Journal of Plant Biology
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• 제14권1호
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• pp.33-35
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• 1971

After topping, axillary buds of haploid plants derived from cultured anthers were treated with 0.4% aqueous solution of colchicine. Due to the high temperature and dry air at the time of treatment, most of the buds perished. A few months after the colchicine application, however, several shoots arose from the places where the dead buds were originally located. These shoots were mostly diploid. Induction of adventive shoots from the colchicine-treatedaxils was supposed to be rather effective method of obtaining diploid shoots from haploid plants. The diploid plants had larger floral organs than the haploid plants, and had good pollen fertility and seed setting. 24 bivalent chromosomes were observed at MI of the PMC's.

• Plant Biotechnology Reports
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• 제5권2호
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• pp.127-134
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• 2011

The biotransformation potential of cell suspension cultures generated from Withania somnifera leaf was investigated, using withanolides, i.e. withanolide A, withaferin A, and withanone as precursor substrates. Interestingly, the cell suspension cultures showed inter-conversion of withanolides, as well converted to some unknown compounds, released to the culture media. The bio-catalyzed withanolide was detected and quantified by TLC and HPLC, respectively. There is noticeable conversion of withanolide A to withanone, and vice versa though at a lower level. The type of reaction of this biotransformation appears to be substitution of 20-OH group to 17-OH in withanolide A. In this paper, we present for the first time the possibility of biotransformation by inter-conversion of withanolides of pharmacological importance through cell suspension culture of W. somnifera. The possible role of putative cytochrome $P_{450}$ hydroxylases is implicated in the conversion.

• Journal of Plant Biotechnology
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• 제1권2호
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• pp.91-95
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• 1999

Genetic engineering of stone fruit species like apricot, plum, peach and cherry are hampered by the inefficient and low-level regeneration processes in tissue culture. The first transgenic stone fruit species have emerged from transformed hypocotyls. These great achievements were applauded by the scientific community contrary the fact that hypocotyl derived transgenic plants have no real brooding value. Tissue culture of different organs of valuable cultivars are recorded with an extremely low-level of regeneration in the literature. To improve the tissue culture basis of stone fruit plants an extensive tissue culture programme were launched and dozens of different media were compared including a series of hormone concentration in the tissue culture systems. Our continuous efforts were crowned by a very efficient method for achieving up to 30-40% regenerable petioles. Usually on a single petiole several well-separated meristems were induced. After 3-4 weeks of cultivation shoots were developed. The basic media $K_2$ were supplemented with 10g/L saccharose, 10g/L glucose and 10g/L maltose. The following plant hormones were used BAP 1mg/L, TDZ 1mg/L, 2-iP 1mg/L and IAA 0,1 mg/L concentrations. The Petri dishes were kept for 3 weeks in dark at a temperature 22$^{\circ}C$ for 8 hours and 22-24$^{\circ}C$ for 16 hours. The Petri dishes were sealed with Parafilm. The regeneration of the petioles were genotype independent and we were able to regenerate different plum cultivars with almost the same efficiency.

• 한국약용작물학회지
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• 제20권5호
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• pp.301-307
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• 2012

IPP isomerase (Iso) and Limonene synthase (Limo) are important enzymes in terpenoids biosynthesis pathway. The wild type and each metabolically engineered (Iso and Limo) transgenic spearmint (Mentha spicata Linne) plants were compared for their growth patterns and the contents of essential oil in in vitro culture media. The profile of terpenoid metabolites was obtained from the essential oil of the metabolically engineered transgenic spearmint, which was extracted using a modified SDE method, by GC-MS analysis. The growth of wild spearmint was more profuse in B5 culture medium than in other media. Significant differences in leaf and root growth patterns were observed between metabolically engineered transgenic and wild type spearmint plants. The leaves of the transgenic spearmint plants were slightly elongated but were dramatically narrower than those of wild type spearmints. The content of essential oil of transgenic spearmint was different slightly depending on the target terpenoid genes. The content of essential oils in Limo transgenic plants was higher than that of Iso, except for transgenic plant in B5 medium. The transgenic spearmint produced more terpenoids than the wild type. Iso spearmint extracts showed eleven terpenoids and a phenylpropane, while Limo spearmint extracts contained nine terpenoids. However, extracts from the wild type showed the presence of only four terpenoids.

• 한국잡초학회지
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• 제8권1호
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• pp.59-63
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• 1988

수도(水稻) 담수직파재배(湛水直播栽培)에서 제초제(除草劑) 처리(處理)에 따라 야기되기 쉬운 약해(藥害)가 안전성(安全性)이 있는 제초제(除草劑)를 선발하기 위하여 이앙답(移秧畓)에서 사용되고 있는 4종(種)의 제초제(除草劑)를 공시(供試)하여 간척답(干拓畓)과 보통답(普通畓)의 토양(土壤)을 사용하여 폿트실험으로 벼의 안전성(安全性)을 조사하였다. 1. Pyrazolate는 300, 600 g ai/10a에서 벼에 안전하여 입모율(立毛率)이 높고 벼의 초장(草長), 경수(莖數) 및 건물중(乾物重)의 감소가 적었다. 2. Bensulfuron methyl 5.1g ai/10a을 파종직후(播種直後)에 처리(處理)하면 약해(藥害)가 유발되었으나 파종(播種) 5 일 후(後)에 처리(處理)하므로써 약해(藥害)가 현저히 감소되었으며, 10.2g ai/10a에서는 다소 약해(藥害)가 크게 나타났다. 3. Chlormethoxynil은 210g ai/10a에서도 약해(藥害)가 나타나 입모율(立毛率)이 낮아지고 벼의 초장(草長), 경수(莖數) 및 건물중(乾物重)도 크게 감소되었다. 4. Benthiocarb는 210g ai/10a을 파종(播種) 5 일(日) 후(後)에 처리(處理)하면 약해(藥害)가 적었으나, 파종직후처리(播種直後處理)와 420g ai/10a 처리(處理)에서는 약해(藥害)가 많은 경향이었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.99-99
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• 2022

Artemisia fukudo is a biennial plant and has been reported to have anticancer, anti-melanogenesis, and anti-inflammatory effects. However, it is difficult to produce biomass from A. fukudo, so it is not used as a material for cosmetics or pharmaceuticals. In vitro culture can stably produce biomass throughout the year. In this study, the culture system for producing the highest biomass and bioactive substances was compared. Ex vitro plants were collected in Pyoseon-eup, Jeju island in May 2021, and in vitro culture was harvested after culturing for 8 weeks (plantlet) and 4 weeks (adventitious roots), respectively. After harvest, total polyphenol content (TPC), total flavonoid content (TFC), and DPPH scavenging activity were analyzed. In biomass production, adventitious roots (FW: 5.1 g·100 ml^-1, DW: 0.6 g·100 ml^-1) were about 4 times higher than that of plantlets (FW: 1.8 g·200 ml^-1, DW: 0.3 g·200 ml^-1). Both TPC and TFC were highest in ex vitro plants (9.2 ㎍·mL^-1, 31.6 ㎍·mL^-1), and were 3.0 times and 1.8 times higher than those of plantlets (3.0 ㎍·mL^-1, 17.8 ㎍·mL^-1), respectively. The IC₅₀ value of DPPH scavenging activity was also the best in ex vitro plants (69.8 ㎍·mL^-1), followed by root root (184.4 ㎍·mL^-1) and plants (325.3 ㎍·mL^-1) in that order. Through additional elicitor treatment, scale-up, and advanced compounds analysis such as HPLC, it can be used as an industrial material.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권2호
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• pp.135-141
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• 2003

The human granulocyte-macrophage colony stimulating factor (hGM-CSF) gene was introduced into tobacco plants. The cell suspension culture was established from leaf-derived calli of the transgenic tobacco plants in order to express and secrete a biologically active hGM -CSF. The recombinant hGM-CSF from the transgenic plant cell culture (prhGM-CSF) was identified as a yield of about 180 ${\mu}$g/L in the culture filtrate, as determined by ELISA. The addition of 0.5 g/L polyvinylpyrrolidone (PVP) to the plant cell culture medium both stabilized the secreted prhGM-CSF and increased the level of production approximately 1.5-fold to 270 ${\mu}$g/L. The biological activity of the prhGM-CSF was confirmed by measuring the proliferation of the hGM-CSF-dependent cell line, TF-1. Interestingly, the specific activity of the prhGM-CSF was estimated to be approximately 2.7 times higher than that of a commercially available preparation from E. coli.