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http://dx.doi.org/10.7783/KJMCS.2012.20.4.301

Comparative Analysis of Terpenoids in in vitro Culture Media of Metabolically Engineered Transgenic and Wild Type Spearmint (Mentha spicata L.)  

Kang, Young-Min (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine ((KIOM))
Park, Dong-Jin (Division of Environmental Forest Secience & Institute of Agriculture and Life Science, Gyeongsang National University)
Song, Hyun-Jin (Division of Environmental Forest Secience & Institute of Agriculture and Life Science, Gyeongsang National University)
Ma, Ho-Seop (Division of Environmental Forest Secience & Institute of Agriculture and Life Science, Gyeongsang National University)
Karigar, Chandrakant (Department of Biochemistry, Bangalore University)
Choi, Myung-Suk (Division of Environmental Forest Secience & Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.20, no.5, 2012 , pp. 301-307 More about this Journal
Abstract
IPP isomerase (Iso) and Limonene synthase (Limo) are important enzymes in terpenoids biosynthesis pathway. The wild type and each metabolically engineered (Iso and Limo) transgenic spearmint (Mentha spicata Linne) plants were compared for their growth patterns and the contents of essential oil in in vitro culture media. The profile of terpenoid metabolites was obtained from the essential oil of the metabolically engineered transgenic spearmint, which was extracted using a modified SDE method, by GC-MS analysis. The growth of wild spearmint was more profuse in B5 culture medium than in other media. Significant differences in leaf and root growth patterns were observed between metabolically engineered transgenic and wild type spearmint plants. The leaves of the transgenic spearmint plants were slightly elongated but were dramatically narrower than those of wild type spearmints. The content of essential oil of transgenic spearmint was different slightly depending on the target terpenoid genes. The content of essential oils in Limo transgenic plants was higher than that of Iso, except for transgenic plant in B5 medium. The transgenic spearmint produced more terpenoids than the wild type. Iso spearmint extracts showed eleven terpenoids and a phenylpropane, while Limo spearmint extracts contained nine terpenoids. However, extracts from the wild type showed the presence of only four terpenoids.
Keywords
GC-MS; In vitro Culture Media; Mentha spicata; Iso; Limo; Spearmint; Terpenoids;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Bienvenu F, Peterson L and Edward J. (1999). Native and scotch spearmint oil production in Tasmania and Victoria. A Report for the Rural Industries Research and Development Corporation. p.99-147.
2 Bohlmann J, Steele CL and Croteau R. (1997). Monoterpene synthases from grand fir (Abiesgrandis). Journal of Biological Chemistry. 272:2178-2192.
3 Caissard JC, Faure O, Jullien F, Colson M and Perrin A. (1996). Direct regeneration in vitro and transient GUS expression in Mentha piperita. Plant Cell Reports. 16:67-70.
4 Choi JE, Nam KY, Li X, Kim BY, Cho HS and Hwang KB. (2010). Changes of chemical compositions and ginsenoside contents of different root parts of ginsengs with processing method. Korean Journal of Medicinal Crop Science. 18:118-125.
5 Choi SR, Kim CS, Kim JY, You DH, Kim JM, Kim YS, Song EJ, Kim YG, Ahn YS and Choi DG. (2011). Changes of antioxidant activity and lignan contents in Schisandra chinensis by harvesting times. Korean Journal of Medicinal Crop Science. 19:414-420.   DOI
6 Colby SM, Alonso WR, Katahira EJ, McGravey DJ and Croteau R. (1993). 4S-limonene synthase from the oil glands of spearmint (Mentha spicata). Journal of Biological Chemistry. 268:16-24.
7 Dudareva N, Cseke L, Blanc VM and Pichersky E. (1996). Evolution of floral scent in Clarkia: novel patterns of S-linalool synthase gene expression in the C. breweri flower. Plant Cell. 8:1137-1148.   DOI
8 Gamborg OL, Miller RA and Ojima K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research. 50:151-158.   DOI   ScienceOn
9 Gershenzon J and Croteau RB. (1993). Terpenoid biosynthesis: the basic pathway and formation of monoterpenes, sesquiterpenes and diterpenes. In Moore TS (ed.) Lipid metabolism in plants. CRC Press. Boca Raton. Florida, USA. p.340-388.
10 Guo J and Wang MH. (2008). Characterization of LeAbsil gene in Lycopersicon esculentum treated with various abiotic and oxidative stresses. Journal of Applied Biological Chemistry. 51:213-214.
11 Kang YM, Min JY, Kim WJ, Kim YD, Lee BH and Choi MS. (2004). Growth pattern and content of tropane alkaloids of metabolic engineered Scopolia parviflora hairy lines. Korean Journal of Medicinal Crop Science. 12:123-128.
12 Lange BM, Mahmoud SS, Wildung MR, Turner GW, Davis EM, Lange I, Baker RC, Boydston RA and Croteau RB. (2011). Improving peppermint essential oil yield and composition by metabolic engineering. Proceedings of the National Academy of Sciences of the United States of America. 108:16944-16949.   DOI   ScienceOn
13 Lewinson O and Bibi E. (2001). Evidence for simultaneous binding of dissimilar substrates by the Escherichia coli multidrug transporter MdfA. Biochemistry. 40:12612-12618.   DOI
14 Linsmaier EM and Skoog F. (1965). Organic growth factor requirements of tobacco tissue culture. Plant Physiology. 21:487-492.
15 Lucker J, El Tamer MK, Schwab W, Verstappen FWA, van der Plas LHW, Bouwmeester HJ and Verhoeven HA. (2002). Monoterpene biosynthesis in lemon(Citrus limon): cDNA isolation and functional analysis of four monoterpene synthases. European Journal of Biochemistry. 269:3160-3171.   DOI
16 Mucciarelli M, Contri P, Monachesi G, Calvano G and Gallipoli MR. (2001). An empirical method to assess the seismic vulnerability of existing buildings using the HVSR technique. Pure and Applied Geophysics. 158:2635-2647.   DOI
17 Murashige T and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 15:473-497.   DOI
18 Nitsch J and Nitsch C. (1969). Haploid plants from pollen grains. Science. 163:85-87.   DOI   ScienceOn
19 Park DJ. (2008). Metabolites profiling and its function through terpenoids biosynthesis genes overexpression of spearmint (Mentha spicata L.). Master Thesis. Gyeongsang National University. p.11-18.
20 Aharoni A, Giri AP, Deuerlein S, Griepink F, de Kogel WJ, Verstappen FWA, Verhoeven HA, Jongsma MA, Schwab W and Bouwmeester HJ. (2003). Terpenoid metabolism in wild-type and transgenic Arabidopsis plants. Plant Cell. 15:2866-2884.   DOI
21 Park WJ, Kang YM, Min JY, Park DJ, Kim YD, Karigar CS and Choi MS. (2004). In vitro propagation of junos orange (Citrus junos Sieb.) through nucellar polyembryoid cultures. Korean Journal of Medicinal Crop Science. 12:384-390.
22 Sacchettini JC and Poulter CD. (1997). Biochemistry-Creating isoprenoid diversity. Science. 277:1788-1789.   DOI
23 Schenk RH and Hildebrandt AC. (1972). Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany. 50:199-204.   DOI
24 Seo WT, Yang JK, Kang BK, Park WJ, Hong SC, Kang YM, Jung HY, Kim YD, Kang SM, Kim SW and Choi MS. (2003). Leaves extraction and biological activities of essential oil from Thuja occidental. Korean Journal of Medicinal Crop Science. 5:364-370.
25 Singh HP, Batish RD and Kohli KR. (1999). Autotoxicity: concept, organisms and ecological significance. Critical Reviews in Plant Sciences. 18:757-772.   DOI   ScienceOn
26 White PR. (1963). The cultivation of animal and plant cells. Ronald Press. New York City. New York, USA. p.60.