Kim, Hyun Sik;Cha, Chang-Jun;Cho, Jang-Cheon;Im, Wan-Taek;Jahng, Kwang Yeop;Jeon, Che Ok;Joh, Kiseong;Kim, Seung Bum;Seong, Chi Nam;Kim, Wonyong;Yi, Hana;Lee, Soon Dong;Yoon, Jung-Hoon;Bae, Jin-Woo
Journal of Species Research
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v.6
no.2
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pp.101-118
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2017
In an investigation of indigenous prokaryotic species in Korea, a total of 57 bacterial strains assigned to the classes Betaproteobacteria and Gammaproteobacteria were isolated from diverse environments. Samples were collected from fresh water, natural caves, soil, paddy fields, lakes, sea water, jeotgal (fermented seafood), salt flats, soil from abandoned mines, plant roots, digestive organs of both Japanese crested ibis (Nipponia nippon) and Burmese python (Python molurus bivittatus) and tidal flats. From the high 16S rRNA gene sequence similarity (>98.7%) and formation of robust phylogenetic clades with closely related species, it was determined that each strain belonged to an independent and predefined bacterial species within either the Betaproteobacteria or Gammaproteobacteria. There is no official report or publication that describes these 57 proteobacterial species in Korea. Overall, in the class Betaproteobacteria there were 16 species in 12 genera of 4 families in the order Burkholderiales and two species in two genera of one family in the order Neisseriales. Within the class Gammaproteobacteia, there were five species in four genera of four families in the order Alteromonadales, 12 species in 11 genera of one family in the order Enterobacteriales, four species in four genera of three families in the order Oceanospirillales, 11 species in four genera of two families in the order Pseudomonadales, two species in the order Vibrionales and five species in five genera of one family in the order Xanthomonadales. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source and strain IDs are described in the species description section.
High-performance liquid chromatography (HPLC) was applied to determine the carotenoid composition of carrots during storage and cooking. Analyses were conducted immediately after harvest and 1, 2, 4, and 8 weeks after harvest. During the course of the storage, the carotenoid levels generally decreased, and this decrease was found to be greater during the first week for $\beta$-carotene (all-trans-$\beta$-carotene) and lutein, and during the second week for $\alpha$-carotene. Additionally, the amount of the $\alpha-$ and $\beta$-carotenes in carrot leaves changed slightly within the first 2 weeks of harvest when stored at $4^{\circ}C$. Specifically, the level of lutein, the main component of carrot leaves, increased from 233.8$\pm$11.7 to $346.2\pm26.7{\mu}g$/g DW during the first 2 weeks. In addition, the change in carotenoid contents was observed during the home-processing of one Korean cultivar. Carrots fried in oil showed the highest amount of $\beta$-carotene ($164.3\pm6.6{\mu}g$/g DW) and $\alpha$-carotene ($50.1\pm0.4{\mu}g$/g DW), while carrots that were prepared by sauteing, pressure-cooking in water and microwaving had the second highest levels. The greatest loss of in carotenoids occurred in response to boiling in water containing 1% NaCl, braising and baking. The content of lutein increased slightly after boiling in water containing 1% NaCl ($9.3\pm0.4{\mu}g$/g DW), while a loss in lutein occurred after preparation using other home-processing methods. A cis-isomer of all-trans-$\beta$-carotene, 13-cis-$\beta$-carotene, was present in detectable amounts in all processed samples, but not in raw roots. Another isomer, 9-cis-$\beta$-carotene, was detected in carrots that were prepared by boiling, frying and pressure-cooking.
Journal of the Korean Institute of Landscape Architecture
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v.29
no.5
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pp.92-100
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2001
This study was conducted to evaluate the growth rate of landscape trees produced in containers for three year, and the growth rate of the establishment period during sixth month~twelve months after transplanting. Two types of container such as plastic pot(pot) and fabric growing bag(bag) were adopted to restrict tree roots. Each type of container was divided into seven sub-types. One traditional production method was included as comparison. Two landscape woody plant species (Magnolia denudata, Albizzia julibrissin) were planted in the seven sub-types of container. After one or two growing season in the container, the trees of each container type were transplanted. Half of the trees were transplanted in the mild spring season, and the other half were transplanted in the improper summer season. The data was collected on the diameter of root cellar and the tree height in each year. The research results are as follows; 1. Container production method was lower than the traditional production methods by 1.3 times ~ 2 times in the growth rates. 2. The Geocell bag and Root control bag revealed higher growth rates than pp-woven bag. And pot- in-pot(double pot)system revealed higher growth rates than the ether container pot system in A denudata. There were no differences in the growth rates between container production system in Albizzia julibrissin. 3, The growth rates of improper transplanting trees was high in the \"pot\" type - \"bag\" type - \"control\" in descending order. Especially, the growth rates of \"pot\" type revealed higher than \"control\" by 4 times. 4. From the results of ANOVA and Ad hoc test, the variable of growth factors of each container types in improper transplanting experiment was not significant for a range of 5% or 1% level. And the growth rates of traditional production method was lower than the container production method. 5. There was no differences in growth rate between the containerized and the traditional production system in the case of proper transplanting experiment. The growth rates of diameter of root collar was higher in the \"control\". The growth rates of tree height was higher in the \"bag\" type. Finally, based upon the results of this study, subsequent research on the development of container materials and maintaining methods that focused on the growth rates would be required.aintaining methods that focused on the growth rates would be required.
This study aimed to examine a suppression effect of salinity on extension rate of stem elongation of cucumber seedlings (Cucumis sativus L.) grafted with roots of figleaf gourd plant. The effects of application methods, timings, and concentrations of two salt types, sodium chloride (NaCl) and calcium chloride ($CaCl_2$), were compared to untreated control plants. In result, an obvious suppression effect on the excessive elongation of stem was obtained by both sub-irrigated and medium-mixed NaCl salt. An improvement in quality of transplants was also obtained by the sub-irrigated NaCl salt. Foliar-applied NaCl caused visible leaf injury when the concentration was higher than 40 mM; but, with no effect on suppressing the stem elongation. When the NaCl was applied at 7 days after grafting, a higher concentration of NaCl was demanded for suppressing the stem elongation compared to an application at the day of grafting. No effect of the NaCl salt on the fresh weights of 36-day grown plants was observed; but, there was a negative effect on the number of female flowers at a high temperature season. Overall, the NaCl salt was more effective on slowing down the stem elongation and had the lower incidence of leaf injury than the $CaCl_2$ salt.
The present study was conducted to characterize physiological responses of aerial part according to soil water stresses in 'Mihong' peach trees. Discolorations, wilting and falling of leaves were observed in chronological order as response for waterlogging and no irrigation and the phenomena started from basal to end of shoots. Shoot growth in elongation and thickness decreased and fallen leaves were severe in waterlogged trees. Function of water uptake by roots and photosynthesis and leaf respiration decreased by waterlogging. Leaf chlorophyll contents decreased in both treatments. In waterlogging treatment, decrease of chlorophyll was observed in normal leaves with waterlogging using light microscopy. Starch content was lower in both treatment and carbohydrate content was lower in root with waterlogging. These results demonstrated that waterlogging weakened the function of soil water uptake and movement and decreased photosynthesis and fallen leaves. Finally the peach trees would wither or suffer low temperature damage through the shortage of reserve accumulations. We suggested that waterlogging damage in peach trees could be reduced to take notice of irrigation and install drainage facility to improve soil condition.
Kim, Myeong Seok;An, Ho Sub;Kim, Gil Ja;Kim, Yong Soon;Choi, Jin Gyung;Park, Heung Gyu;Kim, Hyun Woo;Kim, Seong Il;Kim, Young Guk;Park, Chun Geun;Chang, Jae Gi
Proceedings of the Korean Society of Crop Science Conference
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2017.06a
/
pp.225-225
/
2017
This study is to investigate the effect of split application and adapted fertilization application cultivation in stable medicinal herbs production of Acorus graminens S. And it accomplished one's purpose to GAP guide manual established standard method of cultivation technology, contribute to the enlargement of farm's income. Method of fertilization application were conducted under five condition compose to standard application(N-P-K-Compost: fermented mixed oil cake fertilizer applied at $4-2-6-300kg.10a^{-1}$, twofold application(N-P-K-Compost applied at $8-4-12-600kg.10a^{-1}$) cultivation. Compost (fermented mixed oil cake fertilizer) and fused phosphate applied at 100% of basal fertilizer in April 14. The split application level of standard application and twofold application(N-K) applied at 100%, 50%, 40% of basal fertilizer in April 14, 50% and 40% of top dressing were three times application (N-K) in June 1, July 15, August 15, 25% and 20% respectively. Transplanting with Naju varieties in May 12 by growing pot seedling for 40 days in 2015. Planting density were spaced 30 cm apart in rows 30 cm apart with black vinyl mulching on open cultivation. Plot design randomized block 3 repetition. Therefore, growth characteristics by 50% and 40% of top dressing were three times application (N-K) compared to standard application and twofold application (N-K) applied at 100% basal fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Length of diameter and main root of subterranean part growth increment rising highest. Dried roots yields treated standard fertilizer 100% application($250kg.10a^{-1}$) compared to 100% from twofold application (N-K) applied at 50% basal fertilizer, 50% and 40% of top dressing were three times application increased by 16%, 20%. respectively.
In the current study, antioxidant properties of extracts from different parts of Taraxacum officinale were determined by measuring the radical scavenging activity of 1,1-diphenyl-2-picryl hydrazyl(DPPH) and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The antioxidant activity of aerial and root parts of this plant was measured in extracts obtained from various solvents, e.g., water, 50, 70, 100% ethanol, and hot-water. The hot-water extracts had the highest radical scavenging activities. A further study was conducted to determine the total antioxidant activity of hot-water extract fractions obtained from different solvents, e.g., chloroform, ethyl acetate, n-butanol, and water. The ethyl acetate fraction of this extract displayed the strongest DPPH and ABTS radical scavenging activity and the highest total phenolic contents. The inhibitory effect of individual solvent fractions on the production of nitric oxide in LPS-stimulated RAW 264.7 cells was measured. The results indicated that the ethyl acetate fraction of aerial and roots part extracts significantly reduced nitric oxide productions.
Effect of soil moisture level on the growth of ginger plant was investigated through a pot experiment. The soil used for this study was collected from a newly reclaimed hillside land. The soil was a silty clay loam(19% sand, 57% of silt and 24% of clay), acidic in soil rection(pH 4.7, in $H_2O$) and low in organic matter content(1.2%). Soil moisture levels selected for the experiment were 10, 15, 20, 25, and 30% on weight basis. Under the soil moisture of 20-25%, the emergence ratio was 80-100%, 25 days alter planting. The performance of above ground parts was best under 20-25% of soil moisture. When the soil moisture content was far from 25%, high or low, the die out of above ground parts of ginger tended to increase. Under 20-25% of soil moisture the growth of roots was best and the occurrence of root rot was minimal.
This study was carried out to investigate the optimum management system during a overwintering for the production of Pinus densiflora container 2-0 seedlings. The experiment performed between 2005~2006 in a polyethylene film house (PE house) located at Yeoju-Gun in Kyungki-Do. During the winter in the PE house, the difference in maximum day temperature and minimum day temperature was large, and the difference in temperature was detected between the container keeping locations. During the winter season, the maximum day temperatures at the seedling bench in January and February were $32.8^{\circ}C$ and$36.6^{\circ}C$, respectively, whereas those at the ground in January and February were $16.0^{\circ}C$ and $24.4^{\circ}C$, respectively. Water contents of container seedlings was reduced gradually from the beginning the experiment, and reduced rapidly from February to March, and increased rapidly from April. Container seedlings showed different death rate according to the extending of the irrigation cycle. Death rate by one week and two weeks of irrigation cycle was 4.8% and 6.5%, and 38.5% and 49.4% of death rate occurred by three and four weeks of irrigation cycle, respectively. It is suggested that the proper irrigation cycle for P. densiflora 2-0 container seedlings during overwintering is two weeks. When containers placed directly on the ground, the root of container seedlings went out through the drainage of the container, and grew out in the soil. These roots were cut while moving the container to the bench in spring.
The winter ecology of individual yellow-throated martens(Martes flavigula) intemperate region of Korea were studied through snow-tracking. The study was performed across 3 winter seasons, from January 2011 to February 2013. Total distance of 49.8km was snow tracked (comprising 13 snow-tracking routes) to determine winter foraging habits, general behavior and movement paths of solitary and small groups (1-6 individuals; $mean=2.9{\pm}1.6$) of yellow-throated martens. The martens in the current study were omnivorous, with their winter diet including 9 animal and 5 plant species. Yellow-throated martens searched for food near and under the fallen logs and branches, root plates of fallen trees, around the roots of growing trees, and in small holes in the ground. They also climbed trees to search inside the tree holes and vacant bird nests. Foraging activity was estimated to occur at a frequency of 1.20 times/km, while territory marking occurred 1.42 times/km on average. Of the 60 documented foraging activities we observed, 17 were successful (28.3%). Moving activity and territory marking mainly occurred along ridges, whereas foraging activity was recorded in valleys, slopes, and forest edges. To protect the habitat of this species, the entire forest should be preserved, including the valleys, slopes, and even forest edges as well as main ridges.
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