• Journal of Plant Biotechnology
• /
• v.29 no.1
• /
• pp.19-23
• /
• 2002

This experiment was conducted to micropropagate bulblets via shoot cluster formation and massproduce normal bulblets from the sections of proliferated shoot clusters in Lilium asiatic hybrid 'Hae Hwa'. The induction of shoot clusters from the culture of bulblet sections was more effective than that of bulb scales on MS medium with 1.0 mg/L BA and 0.5 mg/L IAA. Proliferation of shoot clusters from the formed shoot cluster sections was the most favorable on medium containing 5.0 mg/L BA and 0.5 mg/L IAA. The formation and the growth of bulblets from shoot cluster sections were achieved effectively on medium with 60∼90 g/L sucrose. The leaves derived from shoot clusters grew vigorously but the bulblets from shoot clusters grew very poor in 5L air-lift bioreactor culture. By the addition of 30 mL fresh liquid medium containing doulble strength MS salts, 250 g/L sucrose and 5 g/L activated charcoal after 8 weeks in the shoot cluster culture on MS medium with 5.0 mg/L BA and 0.5 mg/L IAA, the number of bulblets was increased in light condition, but the growth of bulblets was not affected by light. Bulblet production was possible with the bulblet product at 53 to 68 mg in fresh weight by liquid medium addition after the proliferation of shoot cluster.

• Korean Journal of Plant Tissue Culture
• /
• v.27 no.3
• /
• pp.185-189
• /
• 2000

For the micropropagation, node explants of cassava were cultured in liquid MS medium with various concentrations of cytokinins on a rotary shaker (100 rpm) for 2 weeks. The adventitious roots and shoots from the explants were differentiated more efficiently in liquid medium than in solid. But root formation was not inhibited in medium with BAP and kinetin at low concentration (＞0.05 mg 1/sup -1/), while in medium added with BAP and zeatin at high level (＜0.25 mg 1/sup -1/), it was inhibited by callus forming on cut end of the cuttings. However, all of plantlets grown in liquid medium for more than 2 weeks showed symptoms of hyperhydricity. The plantlets grown in liquid medium were transferred into culture bottles filled with fine sand or artificial soil (pitmoss：perlite：vermiculite, 1：1：1 v/v) wetted with half strength of Knop's solution. After transplanted to culture bottles, some of vitriscent leaves were defoliated and new leaves were normally formed from shoot apex. Most of plantlets (＞95%) were hardened-off successfully only in culture bottles with fine sand, and grew into 3-5 cm seedlings possessing 4-6 nodes after 4 weeks. Thus, the mass propagation of cassava on medium containing cytokinin could be established based on the suspension culture using node explants.

• Journal of Plant Biotechnology
• /
• v.47 no.1
• /
• pp.46-52
• /
• 2020

This experiment study aimed to identify the continuous genetic variation caused by benzyladenine (BA) treatment in strawberry tissue culture. The 'Goha' cultivar was used and treated with different concentrations of BA (0.0, 0.5, 1.0, 2.0 mg·L^-1). Morphological and genetic variation tests were performed, and genetic continuity tests were performed for three years. The morphological variation induced by BA was distinctively high (10.5 ~ 20.0%) and the genetic variation was 7.0 ~ 15.0%, 1.8 ~ 10.0%, and 5.0% in the first, second, and third year of cultivation, respectively. The rate of genetic variation decreased with increasing cultivation years. In addition, genetic variation caused by BA 1.0 mg·L^-1 and BA 2.0 mg·L^-1 occurred in the first and second years of cultivation, whereas only BA 2.0 mg·L^-1 caused genetic variation in the third year of cultivation. Therefore, a concentration of less than 1.0 mg·L^-1 BA was used for the propagation of strawberry tissue culture plants, and it was necessary to identify their variation.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.24 no.4
• /
• pp.325-330
• /
• 2004

Several kinds of low voltage cables have been used in nuclear power plants for the supply of electric power, supervision, and the propagation of control signals. These low voltage tables must be inspected for safe and stable operation of nuclear power plants. In particular, the degradation diagnosis to estimate the integrity of low voltage rabies has recently been emphasized according to the long use of nuclear power plants. In order to evaluate their degradation, the surrounding temperature, hardness of insulation material, elongation at breaking point (EAB), etc. have been used. However, the measurement of temperature or hardness is not useful because of the absence of quantitative criteria; the inspection of a sample requires turning off of the power plant power; and, the electrical inspection method is not sufficiently sensitive from the initial through the middle stage of degradation. In this research, based on the theory that the ultrasonic velocity changes with relation to the degradation of the material, we measured the ultrasonic velocity as low voltage cables were degraded. To this end, an ultrasonic degradation diagnosis device was developed and used to measure the ultrasonic velocity with the clothing on the cable, and it was confirmed that the ultrasonic velocity changes according to the degradation of low voltage cables. The low voltage cables used in nuclear power plants were degraded at an accelerated rate, and EAB was measured in a tensile test conducted after the measurement of ultrasonic velocity. With the increasing degradation degree, the ultrasonic velocity decreased, whose potential as a useful parameter for the quantitative degradation evaluation was thus confirmed.

• FLOWER RESEARCH JOURNAL
• /
• v.17 no.2
• /
• pp.93-100
• /
• 2009

This study was carried out to investigate the efficient in vitro mass propagation methods for juvenile sporophytes of Matteuccia struthiopteris. Chopped segments of pinnae, petiole and rhizome were cultured on 1/2MS with 0.1% activated charcoal. Among these explant sources only rhizome segments produced young sporophytes, regenerating vigorously on 1/2 MS medium. Adjusting sucrose concentration to 2% and supplement to $50mgL^{-1}$ $NaH_2PO_4$ in 1/2MS medium proved to be more efficient for plant regeneration. Various combinations of growth regulators such as kinetin, BA, NAA, and IBA were added to the growing media, and the best sporophyte regeneration was obtained by $1{\mu}M$ kinetin. The BA addition resulted in vigorous proliferation of meristematic tissues, but without differentiation to sporophytes. Three types of culture methods, solid using agar, liquid stationary, and liquid shaking culture, were employed with or without activated charcoal. The addition of 0.1% activated charcoal to modified 1/2MS media (2% sucrose, $50mgL^{-1}$ $NaH_2PO_4$, $1{\mu}M$ kinetin, pH 5.8 and 0.8% agar) yielded highest sporophyte regeneration in liquid shaking culture.

• FLOWER RESEARCH JOURNAL
• /
• v.17 no.4
• /
• pp.291-296
• /
• 2009

This experiment was conducted to investigate the propagation system by seed to check growth, flowering, and making seed of four-year-old seedling cactus of potted Astrophytum asterias 'Nudum', A. asterias 'Super Kabuto', and A. myriostigma 'Nudum'. Plant height and width of Astrophytum asterias 'Nudum' and A. asterias 'Super Kabuto' was 5.3 to 5.5 cm, and 2.5 cm. However, plant height and width of A. myriostigma 'Nudum' was 7.6 cm and 5.6 cm as divided into 8 sections of body. In A. asterias 'Nudum', the mean seeded pod from April to September was 37.0% and the number of seeds in a pod was 43.0. In A. asterias 'Super Kabuto', the mean seeded pod from April to September was 22.5% and the number of seeds in a pod was 26.2. However, the mean seeded pod of A. myriostigma 'Nudum' from April to September was 32.3% and the number of seeds in a pod was 57.4. Especially, the seeded pod and the number of seeds in a pod of A. asterias 'Nudum' in July was the highest, 72.0% and 66.0. The sib crossing obtained much more seeded pods and the number of seeds per pod than that of selfing in the Astrophytum. The self crossing was formed from 9.0 to 20.0% of seeded pod, and 12.0 to 16.0 seeds in a pod. However, sib crossing was formed 60.0% seeded pod and 68.0 seeds in a pod on July of A. myriostigma 'Nudum'.

• FLOWER RESEARCH JOURNAL
• /
• v.19 no.2
• /
• pp.103-109
• /
• 2011

This study was conducted to develop mass production method by proper cutting time, and the concentrations of plant growth regulators of Spiraea spp. Plants of hard wood was cut on March 3th, the rooting rate in S. salicifolia and S. cantoniensis was 11.3% comparatively higher than that about 5% in S. trichocarpa, S. miyabei, S. japonica, S. thunbergii and S. fritschiana. The rooting of control was 6.0%, however the $125{\sim}1,000mg{\cdot}L^{-1}$ IBA or NAA for 3 hour soaking was above 15.0% in the S. salicifolia. Therefore soaking in $125mg{\cdot}L^{-1}$ of IBA or NAA solution was recommended before cutting. The proper cutting time for soft wood from May 7 to September 17 in S. trichocarpa, S. cantoniensis, S. prunifolia, and S. thunbergii was September 17 when the soft stem was changed hard and the temperate was proper for rooting, high and low, $25{\pm}3^{\circ}C$ and $18{\pm}3^{\circ}C$. The rooting of September 17 was above 70% in S. salicifolia and S. fritschiana. However, the rooting from middle of May to early August was low below 20% in all the genus of Spiraea.

• FLOWER RESEARCH JOURNAL
• /
• v.18 no.1
• /
• pp.29-32
• /
• 2010

This study was conducted to determine the effects of cutting position, media, and auxin on rooting of Artemisia keiskeana cuttings. In the cutting positions, the cutting of middle and basal positions had a better growth in the both upper and lower part than the cutting of the top position. Especially, the cutting of the middle position showed the highest rooting rate, at 68.3%. In the media, peatmoss showed the latest date on the rooting initiation. Also, the growth, survival rate and rooting rate of the upper part and lower part were low. However, the cutting in vermiculate showed the fastest date on the rooting initiation, and had a significant effect on the growth of the upper part and lower part, showing the highest rooting rate and survival rate in all tested media. When cuttings were treated by auxin, the rooting rate and growth of cuttings were higher than the control. The control showed the lowest rooting rate, at 41%, while, the auxin treatment showed the highest, rate over 80%. Whereas when treated by NAA, the lower part of cuttings had a better growth than these treated by IBA. Also, the higher the concentration of auxin was, the higher the rooting rate and growth rate of Artemisia keiskeana cuttings were.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.04a
• /
• pp.57-57
• /
• 2019

본 연구는 관상 및 조경용으로 개발이 가능한 남방계 양치식물인 점고사리[Hypolepis punctata (Thunb.) Mett.]의 전엽체 증식 및 포자체 형성에 적합한 배양조건을 구명하고자 수행되었다. 무가온 온실에서 성숙한 포자엽을 채집한 후 포자를 기내에서 발아시켜 전엽체를 획득하였으며, 8주 간격으로 계대배양 하여 실험의 재료로 사용하였다. 배지종류에 따른 전엽체의 증식 및 형태형성을 확인하고자, 배양된 전엽체 0.3g을 메스로 잘게 다진 후, 농도를 1/4, 1/2, 1, 2배로 조절한 MS배지에 8주간 배양하였다. 이후 선발된 배지를 기준으로 sucrose, 활성탄, 질소급원의 농도를 조절하여 전엽체의 증식과 형태형성을 확인하였다. 그 결과, 1MS배지에서 전엽체의 생체중이 초기 접종량인 0.3g에 비해 10.7배 증가한 3.2g으로 가장 높은 증가율을 보였다. 형태관찰에서도 장정기의 형성이 관찰되었으며, 전엽체의 쿠션조직이 비교적 잘 발달하였다. 전엽체 증식에 가장 좋은 효과를 보인 1MS배지를 기준으로 sucrose의 농도를 0-4%로 달리하여 실험한 결과, 1%의 처리구에서 6.7g으로 가장 높은 생체중을 보였다. 활성탄의 농도를 0-0.8%로 첨가한 네 처리구 중에서는 0.8%의 처리구에서 14.2g으로 무처리구에 비해 생체중이 2배 이상 증가하였다. 전엽체의 형태 또한 정상적인 발달을 보였다. 질소급원의 비율을 30-120mM로 조절한 배지에서는 60mM의 처리구에서 4.9g으로 가장 높은 생체중을보였다. 이후 포자체 형성을 위한 최적의 토양조건을 구명하고자, 원예상토, 피트모스, 펄라이트 및 마사토의 비율을 달리하여 5종류의 배양토를 조성하였다. 혼합된 토양은 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진 하였으며, 배양된 전엽체 1g을 증류수와 함께 10초간 분쇄한 다음 준비된 토양표면에 분주하여 재배하였다. 12주간의 재배 결과, 원예상토를 단용한 토양에서 포자체의 수가 포트당 250.0개로 가장 많이 형성되었으며, 포자체의 생육 또한 다른 처리구에 비해 우수한 결과를 보였다. 한편 피트모스가 혼합된 토양에서는 포자체가 형성되지 않았다. 따라서 점고사리의 전엽체 대량증식에 적합한 배지는 sucrose 1%와 질소급원의 농도를 60mM, 활성탄을 0.8% 첨가한 1MS배지로 판단되며, 포자체 대량생산을 위해서는 원예상토를 단용한 토양이 적합하다고 판단된다.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.04a
• /
• pp.56-56
• /
• 2019

본 연구는 식용과 약용으로 이용되는 국내 자생식물인 야산고비(Onoclea sensibilis var. interrupta Maxim.)를 기내에서 대량증식하기 위한 조건을 구명하기 위하여 수행되었다. 무가온 온실에서 수집한 포자를 기내에서 발아시켜 전엽체를 획득하였으며, 계대배양하며 실험의 재료로 사용하였다. 전엽체의 대량증식을 위해 전엽체 0.3g을 메스로 균일하게 다진 후 증류수 1ml와 함께 배지에 고루 펼쳐서 배양하는 방법을 사용하였으며, 증식에 미치는 배지의 영향을 확인하기 위하여 1/4, 1/2, 1, 2배로 조절한 MS배지를 조성하여 8주간 배양하였다. 이후, 증식이 우수한 배지를 선정하여 sucrose와 질소급원의 농도를 조절하였으며, 활성탄을 첨가하여 증식에 미치는 영향을 확인하였다. 배지종류 실험의 결과, 생체중이 1MS에서 10.2g으로 초기 접종량에 비해 가장 많이 증가하였으며, 현미경을 이용하여 관찰한 결과에서도 정상적인 전엽체의 형태인 심장형으로 발달하였다. 증식이 우수하였던 1MS배지에 sucrose의 농도를 조절하여 배양한 결과에서는 1%의 처리구에서 가장 증식률이 좋았으며, 질소급원의 경우 30mM의 농도로 조절한 처리구가 가장 좋은 결과를 보였다. 배지 내 활성탄의 첨가는 처리구당 증가된 전엽체의 생체중이 유의적인 차이를 보이지 않았다. 포자체 대량 형성을 위한 적정 배양토의 혼합조건을 확인하기 위하여 원예상토, 피트모스, 펄라이트 및 마사토의 혼합비율을 5종류로 달리하여 조성하여 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하였다. 조성한 배양토에 기내에서 배양한 전엽체 1g을 증류수와 함께 블렌더를 이용하여 10초간 분쇄하여 토양표면에 고루 분주하였다. 이후 12주간 재배한 결과, 모든 토양조합에서 포자체가 형성되었다. 그중 원예상토와 마사토를 2:1(v:v)로 혼합한 토양에서 포트 당 405.0개로 가장 많은 포자체가 형성되었으며, 전체적인 생육 또한 비교적 양호한 결과를 보였다. 따라서 야산 고비의 전엽체 대량증식에 적합한 배지는 경제성과 생육수준을 고려하여 1%의 sucrose와 질소급원의 농도를 30mM로 조절한 1MS 배지가 적합하며, 포자체 대량생산을 위해서는 원예상토, 마사토를 2:1(v:v)로 혼합한 토양이 적합하다고 판단되었다.