Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Occurrence and identification of genetic variation and variation continuity in strawberry tissue culture caused by benzyladenine treatment

딸기 조직배양 시 BA (benzyladenine) 처리에 따른 변이 발생 및 변이 연속성 검정

  • Kim, Hye Jin (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Choi, Mi Ja (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Lee, Jong Nam (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Suh, Jong Taek (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Kim, Ki Deog (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Kim, Yul Ho (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Hong, Su Young (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Kim, Su Jeong (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Sohn, Hwang Bae (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Nam, Jeong Hwan (Highland Agricultural Research Institute, National Institute of Crop Science)
  • 김혜진 (국립식량과학원 고령지농업연구소) ;
  • 최미자 (국립식량과학원 고령지농업연구소) ;
  • 이종남 (국립식량과학원 고령지농업연구소) ;
  • 서종택 (국립식량과학원 고령지농업연구소) ;
  • 김기덕 (국립식량과학원 고령지농업연구소) ;
  • 김율호 (국립식량과학원 고령지농업연구소) ;
  • 홍수영 (국립식량과학원 고령지농업연구소) ;
  • 김수정 (국립식량과학원 고령지농업연구소) ;
  • 손황배 (국립식량과학원 고령지농업연구소) ;
  • 남정환 (국립식량과학원 고령지농업연구소)
  • Received : 2020.01.29
  • Accepted : 2020.03.13
  • Published : 2020.03.31
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Abstract

This experiment study aimed to identify the continuous genetic variation caused by benzyladenine (BA) treatment in strawberry tissue culture. The 'Goha' cultivar was used and treated with different concentrations of BA (0.0, 0.5, 1.0, 2.0 mg·L-1). Morphological and genetic variation tests were performed, and genetic continuity tests were performed for three years. The morphological variation induced by BA was distinctively high (10.5 ~ 20.0%) and the genetic variation was 7.0 ~ 15.0%, 1.8 ~ 10.0%, and 5.0% in the first, second, and third year of cultivation, respectively. The rate of genetic variation decreased with increasing cultivation years. In addition, genetic variation caused by BA 1.0 mg·L-1 and BA 2.0 mg·L-1 occurred in the first and second years of cultivation, whereas only BA 2.0 mg·L-1 caused genetic variation in the third year of cultivation. Therefore, a concentration of less than 1.0 mg·L-1 BA was used for the propagation of strawberry tissue culture plants, and it was necessary to identify their variation.

본 실험은 딸기 조직배양 시 BA 처리에 따른 변이 발생 및 변이 연속성을 확인하고자 실시하였다. 본 실험에 사용된 공시 품종은 '고하'이며, 본 실험에 사용한 BA 농도는 0.0, 0.5, 1.0, 2.0 mg·L-1로 처리하였다. 변이는 형태적, 유전적 검정을 실시하였으며, 변이 연속성 검정은 3년간 실시하였다. BA 처리 시 형태적 변이는 10.5 ~ 20.0%로 매우 높게 나타났으나, 유전적 변이는 재배 1년차에 7.0 ~ 15.0%, 재배 2년차에는 1.8 ~ 10.0%, 재배 3년차에는 5.0%로 재배연수가 길어짐에 따라 유전적 변이 발생율이 낮아졌다. 뿐만 아니라 재배1년차와 2년차에는 BA 1.0 mg·L-1과 BA 2.0 mg·L-1에서 유전적 변이가 발생한 반면, 재배 3년차에는 BA 2.0 mg·L-1에서만 유전적 변이가 발생하였다. 따라서 딸기 조직배양묘의 증식을 위해서 BA는 1.0 mg·L-1미만으로 처리하고, 반드시 변이 검정 후 보급하는 것이 바람직하다고 판단되었다.

Keywords

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