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http://dx.doi.org/10.5010/JPB.2020.47.1.046

Occurrence and identification of genetic variation and variation continuity in strawberry tissue culture caused by benzyladenine treatment  

Kim, Hye Jin (Highland Agricultural Research Institute, National Institute of Crop Science)
Choi, Mi Ja (Highland Agricultural Research Institute, National Institute of Crop Science)
Lee, Jong Nam (Highland Agricultural Research Institute, National Institute of Crop Science)
Suh, Jong Taek (Highland Agricultural Research Institute, National Institute of Crop Science)
Kim, Ki Deog (Highland Agricultural Research Institute, National Institute of Crop Science)
Kim, Yul Ho (Highland Agricultural Research Institute, National Institute of Crop Science)
Hong, Su Young (Highland Agricultural Research Institute, National Institute of Crop Science)
Kim, Su Jeong (Highland Agricultural Research Institute, National Institute of Crop Science)
Sohn, Hwang Bae (Highland Agricultural Research Institute, National Institute of Crop Science)
Nam, Jeong Hwan (Highland Agricultural Research Institute, National Institute of Crop Science)
Publication Information
Journal of Plant Biotechnology / v.47, no.1, 2020 , pp. 46-52 More about this Journal
Abstract
This experiment study aimed to identify the continuous genetic variation caused by benzyladenine (BA) treatment in strawberry tissue culture. The 'Goha' cultivar was used and treated with different concentrations of BA (0.0, 0.5, 1.0, 2.0 mg·L-1). Morphological and genetic variation tests were performed, and genetic continuity tests were performed for three years. The morphological variation induced by BA was distinctively high (10.5 ~ 20.0%) and the genetic variation was 7.0 ~ 15.0%, 1.8 ~ 10.0%, and 5.0% in the first, second, and third year of cultivation, respectively. The rate of genetic variation decreased with increasing cultivation years. In addition, genetic variation caused by BA 1.0 mg·L-1 and BA 2.0 mg·L-1 occurred in the first and second years of cultivation, whereas only BA 2.0 mg·L-1 caused genetic variation in the third year of cultivation. Therefore, a concentration of less than 1.0 mg·L-1 BA was used for the propagation of strawberry tissue culture plants, and it was necessary to identify their variation.
Keywords
Benzyladenine; Fragaria ${\times}$ ananassa; Genetic variation; Morphological variation;
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Times Cited By KSCI : 2  (Citation Analysis)
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