• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.445-460
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• 2017

This study was carried out for three years from 2013 to 2015 to investigate the actual condition of pest control of domestic persimmon organically cultivated farmers, to select environment - friendly pest control materials for major pests, and to conduct field test of established control system. The main cultivated cultivar of the reader farmer of organic cultivation was "Buyou" and sprayed organic agricultural material 9~17 times per year for pest control. The most harmful pests were anthracnose, bugs, persimmon fruit worm, and scales, etc. The control materials used were organic lime sulfur mixture, red clay sulfur, emulsifier, bordeaux solution, and self-made plant extracts using garlic, ginkgo nut and pine tree. The effective materials for controlling anthrax were lime sulfur mixture and red clay sulfur. Garlic oil + citronella emulsion, shrubby sophora seed extract + tea extract was effective to control Riptortus clavatus and Euproctis subflava. When installed 60 ea per 10a of mating disruption trap in a sweet orchard, the fruit setting rate was improved by 30%. The results of field test of control system to control 10 times a year in organically grown persimmons were able to harvest fruit of 70.7%. However, since any kinds of pesticides can not be sprayed during the harvest season in September and October, the damaged by bugs did not decreased, and further research is needed.

• Korean Journal of Medicinal Crop Science
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• v.12 no.1
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• pp.43-46
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• 2004

In genus Angelica, three species have been used and cultivated for medicinal material in orient, A. gigas in Korea, acutiloba in Japan and sinensis in China. A. gigas (Chamdangui) is different from A. acutiloba or A. sinensis in the contents chemical components and their efficacious. In Korea, in particular, A. acutiloba has been cultivated in a small acreage from several decades ago, and recently A. sinensis has been trying to cultivate. A trial of adaptation and cultivation of the three species was performed in different altitudinal regions such as Suwon (50 m of sea level), Jinbu (500 m of sea level) and Taebak (700 m of sea level). A. gigas and acutiloba grew and produced roots successfully at all locations, but A. sinensis could grow only at high altitudinal region, Taebaek. A. sinensis survived 70.3% at Taebaek, but only 45.2% at Jinbu and 3.6% at Suwon. The dry root yield of A. sinensis was as low as 1,600 kg/ha at Taebaek and 1,270 kg/ha at Jinbu comparing farmers' average root yield of A. gigas, 3,000 kg/ha however, no root could be harvested at Suwon. The results, showed it was that A. sinensis could not grow at low altitudinal plane area except high altitude area in Korea. Even though it could grow, the root yield was too low to support the income.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.4
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• pp.464-469
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• 2015

This study was carried out to find relationships of genetic distance and heterosis expression degree in the developed CNU waxy corn lines. The used material were developed in Corn Breeding Laboratory, Coll. of Agri. & Life Sci., CNU. Total 10 primers used for SSR maker analysis. In genetic distance, the used lines divided into four groups; A group has two lines, B group has one line, C group has ten lines and D group has eleven lines, respectively. Among used lines, CNU427 and CNU588 were very closed as a 74, while CNU451 and CNU429 were remote as a 40 inbred coefficient, respectively. While heterosis degree were very variable not only hybrids but also cross parents. Average heterosis of most cross-parent was high in plant height, ear height, flowering day and ear length. Especially, CNU H09-23 hybrid was high as 79.4% than other hybrids. Here, we gained the fact has closely relationship between genetic distance and heterosis.

• Korean Journal of Environmental Agriculture
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• v.20 no.1
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• pp.8-14
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• 2001

To investigate the effects of waste sludge from antibiotic fermentation on the growth of young radish and chemical properties of soil, five levels of fertilizer, control (recommended fertilizer, $N-P_2O_5-K_2O=160-59-104$ kg/ha), AS(control + sludge 1,000 kg/ha), AC(control + conventional compost 1,000 kg/ha), SNS(control - subtracting 30% N of sludge + sludge 1,000 kg/ha) and SNC(control - subtracting 30% N of conventional compost + conventional compost 1,000 kg/ha) were applied and radish was grown twice with same treatments on May and August in 1998. Germination rate and early growth of young radish grown with AS and SNS were lower than those grown in control and with AC, SNC. This negative effects by adding the sludge in the early growth seemed to be caused by damage of ammonia gas released during degradation of the sludge in soil. However, yield of young radish showed no significant difference among all the treatments including the AS and SNS at the 1st and 2nd experiment, and these suggested that the latter half of growth of young radish was accelerated by adding the sludge. Contents of T-N in young radish and inorganic N in soil showed a tendency to increase by adding the sludge while antibiotic substance, cephalosporin-C, was not detected in plant material and soils after harvest of young radish in both experiments. Consequently, waste sludge from antibiotic fermentation, which contains high levels of organic matter and nitrogen could be used as an useful resource in agriculture.

• Korean Journal of Head & Neck Oncology
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• v.25 no.1
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• pp.3-7
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• 2009

Background and Objectives : Panax ginseng C.A. Meyer is a medical plant that has been widely utilized as a tonic and nutritional agent since ancient times in Korea. Ginseng has anti-metastatic property of cancer and immunomodulating activity. The novel acidic polysaccharide compound(MB40) was isolated from the leaves of Panax ginseng C.A. Meyer. To determine immunomodulating activities of MB40, we evaluate anti-cancer and anti-metastatic effects of MB40 in tumor bearing immune competent mice. Material and Methods : C3H mice were divided into three equal groups(Cisplatin treatment group, MB40 treat-ment group, Cisplatin and MB40 treatment group) and were transplanted SCC(Squamous Cell Carcinoma) cells(2${\times}$106) to the lateral side of abdomen. From day 4 after transplantation, MB40 was administrated at dose of 10mg/kg, respectively, every other day by intratumoral injection. Cisplatin was systemically administrated at doses of 1mg/kg, respectively, every week by intraperitoneal injection. Results : 5 days after administration, tumors can be palpated in every mice group. After 13 days of administration, the mice group to which MB40 were administrated exhibited reduction in tumor size respectively, compared to cisplatin group. Overall status of mice such as body weight and activity were superior in MB40 group than cisplatin group. Conclusion : The result of this study indicates MB40 may have significant therapeutic effect and decreases complications induced by systemic chemotheraphy. MB40 may be developed as a novel and potent immunotropics to improve the cell immune system and anti-cancer drug for the treatment of cancer patients in head and neck squamous cell carcinoma.

• Journal of the Korean Society of Hazard Mitigation
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• v.8 no.3
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• pp.51-58
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• 2008

Development of a new design guide which is based on empirical-mechanistic concept for pavement design is in action. It is called AASHTO 2002 Design Guide in USA and the KPRP(Korean Pavement Research Project) in Korea. The material characteristic of hot mix asphalt is a key role in the design guide. Therefore it is urgent to get a proper materials database, especially the dynamic modulus of hot mix asphalt. In this research, dynamic modulus test, which is based on aged asphalt binder, has been carried out and proposed the predicted equation of dynamic modulus. Nine different hot mix asphalt with three different asphalt binder have been used for the dynamic modulus test. Short-term aging, which is covers the time for the production of asphalt plant, transportation, lay-down, and compaction, can be simulated at $135^{\circ}C$ with 2 hour curing. Long-term aging has been carried out for a performance period of asphalt pavement. The dynamic modulus of asphalt pavement increases with aging time. As the nominal aggregate size increases, the change of dynamic modulus is not big.

• Korean Journal of Environmental Agriculture
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• v.31 no.4
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• pp.368-374
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• 2012

BACKGROUND: The urgency of feeding the world's growing population while combating soil pollution, salinization and desertification requires suitable biotechnology not only to improve crop productivity but also to improve soil health through interactions of soil nutrient and soil microorganism. Interest in the utilization of microbial fertilizer has increased. A principle of nature farming is to produce abundant and healthy crops without using chemical fertilizer and pesticides, and without interrupting the natural ecosystem. Beneficial microorganisms may provide supplemental nutrients in the soil, promote crop growth, and enhance plant resistance against pathogenic microorganisms. We mixed beneficial microorganisms such as Bacillus sp. Han-5 with anti-fungal activities, Trichoderma harziaum, Trichoderma longibrachiatum with organic material degrading activity, Actinomycetes bovis with antibiotic production and Pseudomonas sp. with nitrogen fixation. This study was carried out to investigate the mixtures on the soil microflora and soil chemical properties and the effect on the growth of lettuce and cucumber under greenhouse conditions. METHODS AND RESULTS: The microbial mixtures were used with each of organic fertilizer, swine manure and organic+swine manure and compared in regard to changes in soil chemical properties, soil microflora properties and crop growth. At 50 days after the treatment of microorganism mixtures, the pH improved from 5.8 to 6.3, and the EC, $NO_3$-Na and K decreased by 52.4%, 60.5% and 29.3%, respectively. The available $P_2O_5$ and $SiO_2$ increased by 25.9% and 21.2%, respectively. Otherwise, the population density of fluorescent Pseudomonas sp. was accelerated and the growth of vegetables increased. Moreover, the population density of E. coli and Fusarium sp., decreased remarkably. The ratio of bacteria to fungi (B/F) and the ratio of Actinomycetes bovis to fungi (A/F) increased 2.3 (from 272.2 to 624.4) and 1.7 times (from 38.3 to 64), respectively. Furthermore, the growth and yield of cucumber and lettuce significantly increased by the treatment of microorganism mixtures. CONCLUSION(S): These results suggest that the treatment of microorganism mixtures improved the chemical properties and the microflora of soil and the crop growth. Therefore, it is concluded that the microorganism mixtures could be good alternative soil amendments to restore soil nutrients and soil microflora.

• Journal of Life Science
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• v.27 no.11
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• pp.1233-1244
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• 2017

CDPKs have pivotal roles in plant $Ca^{2+}$-mediated transduction signaling. A total of 29 CDPK genes have been identified in rice (Oryza sativa L.), but their key functions have not been completely noted. This study focused on the OsCPK11 gene, which has not been studied, to determine its functional characteristics. A study of tissue-specific expressions revealed that the OsCPK11 gene is expressed in young leaves, mature leaves and flowers of rice. An expression of the gene was also confirmed in gibberellin-treated aleurone layers of rice. Regarding the phenotypic characteristics of Tos17-inserted OsCPK11 mutants, the heights of the mutants were not distinguishable from the heights of wild type plants, but the number of caryopses and the caryopses' weights were significantly statistically different. In addition, many grains of the mutants had white belly materials in their endosperm. The cDNA of the OsCPK11 was cloned, and an OsCPK11 protein of about 60.5 kD was obtained by using a GST affinity chromatography and an SDS-PAGE. An analysis of the amino-acid sequence of the protein indicated that the OsCPK11 protein has the structural characteristics of typical CDPKs. The results provided useful information about the functions of the OsCPK11 gene and further noted the roles CDPKs have in $Ca^{2+}$-mediated signaling in plants.

• Journal of the Korea Institute of Building Construction
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• v.13 no.2
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• pp.122-130
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• 2013

One of recent methods to protect wooden cultural assets from fire, there is the flame retardation which is applied directly to wood and it is to prevent fire through securing flame resistance for the material and delaying combustion when failed fire in advance and then to gain time for people in the room to evacuate and it has same goal with the Korean Fire Service Act by protecting life and property. However, in case of spraying flame retardant on the colored surface of the wooden cultural assets, there are continuous problems of decoloration, efflorescence and water absorbtion after sometime and accordingly there increases danger of damages of cultural assets. So when treating with flame retardant on wooden cultural assets, there has to be no problems on dancheong after sometime and securing sustainable methods for flmae retardation should be preceded. Accordingly, this study aims to provide basic sources for selecting proper flame retardation methods by evaluating and analyzing flame retardation capabilities according to types of flame retardants which are frequently used nowadays and spraying them on the dancheong-painted surface and confirming if there is no problem on the dancheong and wood after sometime and if flame retardation effect is sustainable with its quality and capability through precise analysis.

• 한국균학회소식:학술대회논문집
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• 2015.11a
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• pp.41-41
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• 2015

Oomycetes belong to the kingdom Straminipila, a remarkably diverse group which includes brown algae and planktonic diatoms, although they have previously been classified under the kingdom Fungi. These organisms have evolved both saprophytic and pathogenic lifestyles, and more than 60% of the known species are pathogens on plants, the majority of which are classified into the order Peronosporales (includes downy mildews, Phytophthora, and Pythium). Recent phylogenetic investigations based on DNA sequences have revealed that the diversity of oomycetes has been largely underestimated. Although morphology is the most valuable criterion for their identification and diversity, morphological species identification is time-consuming and in some groups very difficult, especially for non-taxonomists. DNA barcoding is a fast and reliable tool for identification of species, enabling us to unravel the diversity and distribution of oomycetes. Accurate species determination of plant pathogens is a prerequisite for their control and quarantine, and further for assessing their potential threat to crops. The mitochondrial cox2 gene has been widely used for identification, taxonomy and phylogeny of various oomycete groups. However, recently the cox1 gene was proposed as a DNA barcode marker instead, together with ITS rDNA. To determine which out of cox1 or cox2 is best suited as universal oomycete barcode, we compared these two genes in terms of (1) PCR efficiency for 31 representative genera, as well as for historic herbarium specimens, and (2) in terms of sequence polymorphism, intra- and interspecific divergence. The primer sets for cox2 successfully amplified all oomycete genera tested, while cox1 failed to amplify three genera. In addition, cox2 exhibited higher PCR efficiency for historic herbarium specimens, providing easier access to barcoding type material. In addition, cox2 yielded higher species identification success, with higher interspecific and lower intraspecific divergences than cox1. Therefore, cox2 is suggested as a partner DNA barcode along with ITS rDNA instead of cox1. Including the two barcoding markers, ITS rDNA and cox2 mtDNA, the multi-locus phylogenetic analyses were performed to resolve two complex clades, Bremia lactucae (lettuce downy mildew) and Peronospora effuse (spinach downy mildew) at the species level and to infer evolutionary relationships within them. The approaches discriminated all currently accepted species and revealed several previously unrecognized lineages, which are specific to a host genus or species. The sequence polymorphisms were useful to develop a real-time quantitative PCR (qPCR) assay for detection of airborne inoculum of B. lactucae and P. effusa. Specificity tests revealed that the qPCR assay is specific for detection of each species. This assay is sensitive, enabling detection of very low levels of inoculum that may be present in the field. Early detection of the pathogen, coupled with knowledge of other factors that favor downy mildew outbreaks, may enable disease forecasting for judicious timing of fungicide applications.