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The Anti-tumor Effect of Polysaccharide from the Leaves of Panax Ginseng C.A. Meyer(MB40) in a Murine Squamous Cell Carcinoma Model  

Joo, Eun-Jung (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine)
Choi, Jun (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine)
Chung, Eun-Jae (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine)
Hong, Seok-Jin (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine)
Cho, Jae-Gu (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine)
Baek, Seung-Kuk (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine)
Woo, Jeong-Soo (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine)
Jung, Kwang-Yoon (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine)
Kwon, Soon-Young (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine)
Publication Information
Korean Journal of Head & Neck Oncology / v.25, no.1, 2009 , pp. 3-7 More about this Journal
Abstract
Background and Objectives : Panax ginseng C.A. Meyer is a medical plant that has been widely utilized as a tonic and nutritional agent since ancient times in Korea. Ginseng has anti-metastatic property of cancer and immunomodulating activity. The novel acidic polysaccharide compound(MB40) was isolated from the leaves of Panax ginseng C.A. Meyer. To determine immunomodulating activities of MB40, we evaluate anti-cancer and anti-metastatic effects of MB40 in tumor bearing immune competent mice. Material and Methods : C3H mice were divided into three equal groups(Cisplatin treatment group, MB40 treat-ment group, Cisplatin and MB40 treatment group) and were transplanted SCC(Squamous Cell Carcinoma) cells(2${\times}$106) to the lateral side of abdomen. From day 4 after transplantation, MB40 was administrated at dose of 10mg/kg, respectively, every other day by intratumoral injection. Cisplatin was systemically administrated at doses of 1mg/kg, respectively, every week by intraperitoneal injection. Results : 5 days after administration, tumors can be palpated in every mice group. After 13 days of administration, the mice group to which MB40 were administrated exhibited reduction in tumor size respectively, compared to cisplatin group. Overall status of mice such as body weight and activity were superior in MB40 group than cisplatin group. Conclusion : The result of this study indicates MB40 may have significant therapeutic effect and decreases complications induced by systemic chemotheraphy. MB40 may be developed as a novel and potent immunotropics to improve the cell immune system and anti-cancer drug for the treatment of cancer patients in head and neck squamous cell carcinoma.
Keywords
Polysaccharide; Leaves; Panax ginseng; Squamous cell carcinoma;
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1 Xie FY, Zeng ZF, Huang HY. Clinical observation on nasopharyngeal carcinoma treated with combined therapy of radiotherapy and ginseng polysaccharide injection. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2001;21:332-334   PUBMED
2 Popov AM, Atopkina LN, Uvarova NI, Elyakov GB. The antimetastatic and immunomodulating activities of ginseng minor glycosides. Dokl Biochem Biophys. 2001;380:309-312   DOI   ScienceOn
3 Louis JA, Chiller JM, Weigle WO. The ability of bacterial lipopolysaccharide to modulate the induction of unresponsiveness to a state of immunity. Cellular parameters. J Exp Med. 1973;138:1481-1495   DOI   ScienceOn
4 Sung H, Kang SM, Lee MS, Kim TG, Cho YK. Korean red ginseng slows depletion of CD4 T cells in human immunodeficiency virus type 1-infected patients. Clin Diagn Lab Immunol. 2005;12:497-501   DOI   PUBMED   ScienceOn
5 O'Malley BW Jr, Cope KA, Johnson CS, Schwartz MR. A new immunocompetent murine model for oral cancer. Arch Otolarylgol Head Neck Surg. 1997;123:20-24   DOI   ScienceOn
6 Anzai R, Hashimoto W, Sugiura K, Takahashi M, Satoh M, Kumagai K. Liver NK1.1+ CD4+ alpha beta T cells activated by IL- 12 as a major effector in inhibition of experimental tumor metastasis. J Immunol. 1996;156:3366-3373   PUBMED
7 Vinogradov EV, Muller-Loennies S, Petersen BO, Meshkov S, Thomas-Oates JE, Holst O, et al. Structural investigation of the lipopolysaccharide from Acinetobacter haemolyticus strain NCTC 10305 (ATCC 17906, DNA group 4). Eur J Biochem. 1997;247:82-90   DOI   ScienceOn
8 Yoshikawa M, Morikawa T, Yashiro K, Murakami T, Matsuda H. Bioactive saponins and glycosides. XIX. Notoginseng (3): Immunological adjuvant activity of notoginsenosides and related saponins: Structures of notoginsenosides-L, -M, and -N from the roots of Panax notoginseng (Burk.) F.H. Chen. Chem Pharm Bull (Tokyo) 2001;49:1452-1456   DOI   ScienceOn
9 Letessier EM, Sacchi M, Johnson JT, Herberman RB, Whiteside TL. The absence of lymphoid suppressor cells in tumor-involved lymph nodes of patients with head and neck cancer. Cell Immunol.1990;130:446-458   DOI   ScienceOn
10 Herberman RB. Possible role of natural killer cells and other effector cells in immune surveillance against cancer. J Invest Dermatol.1984;83(Suppl):137-140   DOI   PUBMED
11 Lee YS, Chung IS, Lee IR, Kim KH, Hong WS, Yun YS. Activation of multiple effector pathways of immune system by the antineoplastic immunostimulator acidic polysaccharide ginsan isolated from Panax ginseng. Anticancer Res. 1997;17:323-331   PUBMED
12 Hashimoto W, Takeda K, Anzai R, Ogasawara K, Sakihara H, Sugiura K. Cytotoxic NK1.1 Ag+ alpha beta T cells with intermediate TCR induced in the liver of mice by IL-12. J Immunol. 1995; 154:4333-4340   PUBMED
13 Attele AS, Wu JA, Yuan CS. Ginseng pharmacology: Multiple constituents and multiple actions. Biochem Pharmacol. 1999;58:1685-1693   DOI   ScienceOn
14 Smolina TP, Solov'eva TF, Besednova NN. Immunotropic activity of panaxans-bioglycans isolated from ginseng. Antibiot Khimioter. 2001;46:19-22   PUBMED
15 Yoshikawa M, Morikawa T, Kashima Y, Ninomiya K, Matsuda H. Structures of new dammarane-type Triterpene Saponins from the flower buds of Panax notoginseng and hepatoprotective effects of principal Ginseng Saponins. J Nat Prod. 2003;66:922-927   DOI   ScienceOn
16 Li J, Huang M, Teoh H, Man RY. Panax quinquefolium saponins protects low density lipoproteins from oxidation. Life Sci. 1999; 64:53-62   DOI   ScienceOn