Korean Journal of Head & Neck Oncology (대한두경부종양학회지)

The Korean Society for Head and Neck Oncology (대한두경부종양학회)
권호 표지

The Anti-tumor Effect of Polysaccharide from the Leaves of Panax Ginseng C.A. Meyer(MB40) in a Murine Squamous Cell Carcinoma Model

편평세포암 동물 모델에서 고려인삼잎-다당체(MB40)의 항암치료효과

  • Joo, Eun-Jung (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine) ;
  • Choi, Jun (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine) ;
  • Chung, Eun-Jae (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine) ;
  • Hong, Seok-Jin (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine) ;
  • Cho, Jae-Gu (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine) ;
  • Baek, Seung-Kuk (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine) ;
  • Woo, Jeong-Soo (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine) ;
  • Jung, Kwang-Yoon (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine) ;
  • Kwon, Soon-Young (Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine)
  • 주은정 (고려대학교 의과대학 이비인후과학교실) ;
  • 최준 (고려대학교 의과대학 이비인후과학교실) ;
  • 정은재 (고려대학교 의과대학 이비인후과학교실) ;
  • 홍석진 (고려대학교 의과대학 이비인후과학교실) ;
  • 조재구 (고려대학교 의과대학 이비인후과학교실) ;
  • 백승국 (고려대학교 의과대학 이비인후과학교실) ;
  • 우정수 (고려대학교 의과대학 이비인후과학교실) ;
  • 정광윤 (고려대학교 의과대학 이비인후과학교실) ;
  • 권순영 (고려대학교 의과대학 이비인후과학교실)
  • Published : 2009.05.22
Download PDF
⟨ Previous Next ⟩

Abstract

Background and Objectives : Panax ginseng C.A. Meyer is a medical plant that has been widely utilized as a tonic and nutritional agent since ancient times in Korea. Ginseng has anti-metastatic property of cancer and immunomodulating activity. The novel acidic polysaccharide compound(MB40) was isolated from the leaves of Panax ginseng C.A. Meyer. To determine immunomodulating activities of MB40, we evaluate anti-cancer and anti-metastatic effects of MB40 in tumor bearing immune competent mice. Material and Methods : C3H mice were divided into three equal groups(Cisplatin treatment group, MB40 treat-ment group, Cisplatin and MB40 treatment group) and were transplanted SCC(Squamous Cell Carcinoma) cells(2${\times}$106) to the lateral side of abdomen. From day 4 after transplantation, MB40 was administrated at dose of 10mg/kg, respectively, every other day by intratumoral injection. Cisplatin was systemically administrated at doses of 1mg/kg, respectively, every week by intraperitoneal injection. Results : 5 days after administration, tumors can be palpated in every mice group. After 13 days of administration, the mice group to which MB40 were administrated exhibited reduction in tumor size respectively, compared to cisplatin group. Overall status of mice such as body weight and activity were superior in MB40 group than cisplatin group. Conclusion : The result of this study indicates MB40 may have significant therapeutic effect and decreases complications induced by systemic chemotheraphy. MB40 may be developed as a novel and potent immunotropics to improve the cell immune system and anti-cancer drug for the treatment of cancer patients in head and neck squamous cell carcinoma.

Keywords

References

  1. Li J, Huang M, Teoh H, Man RY. Panax quinquefolium saponins protects low density lipoproteins from oxidation. Life Sci. 1999; 64:53-62 https://doi.org/10.1016/S0024-3205(98)00533-5
  2. Xie FY, Zeng ZF, Huang HY. Clinical observation on nasopharyngeal carcinoma treated with combined therapy of radiotherapy and ginseng polysaccharide injection. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2001;21:332-334
  3. Smolina TP, Solov'eva TF, Besednova NN. Immunotropic activity of panaxans-bioglycans isolated from ginseng. Antibiot Khimioter. 2001;46:19-22
  4. Popov AM, Atopkina LN, Uvarova NI, Elyakov GB. The antimetastatic and immunomodulating activities of ginseng minor glycosides. Dokl Biochem Biophys. 2001;380:309-312 https://doi.org/10.1023/A:1012327806554
  5. Sung H, Kang SM, Lee MS, Kim TG, Cho YK. Korean red ginseng slows depletion of CD4 T cells in human immunodeficiency virus type 1-infected patients. Clin Diagn Lab Immunol. 2005;12:497-501 https://doi.org/10.1128/CDLI.12.4.497-501.2005
  6. Yoshikawa M, Morikawa T, Kashima Y, Ninomiya K, Matsuda H. Structures of new dammarane-type Triterpene Saponins from the flower buds of Panax notoginseng and hepatoprotective effects of principal Ginseng Saponins. J Nat Prod. 2003;66:922-927 https://doi.org/10.1021/np030015l
  7. Yoshikawa M, Morikawa T, Yashiro K, Murakami T, Matsuda H. Bioactive saponins and glycosides. XIX. Notoginseng (3): Immunological adjuvant activity of notoginsenosides and related saponins: Structures of notoginsenosides-L, -M, and -N from the roots of Panax notoginseng (Burk.) F.H. Chen. Chem Pharm Bull (Tokyo) 2001;49:1452-1456 https://doi.org/10.1248/cpb.49.1452
  8. Vinogradov EV, Muller-Loennies S, Petersen BO, Meshkov S, Thomas-Oates JE, Holst O, et al. Structural investigation of the lipopolysaccharide from Acinetobacter haemolyticus strain NCTC 10305 (ATCC 17906, DNA group 4). Eur J Biochem. 1997;247:82-90 https://doi.org/10.1111/j.1432-1033.1997.00082.x
  9. Louis JA, Chiller JM, Weigle WO. The ability of bacterial lipopolysaccharide to modulate the induction of unresponsiveness to a state of immunity. Cellular parameters. J Exp Med. 1973;138:1481-1495 https://doi.org/10.1084/jem.138.6.1481
  10. Letessier EM, Sacchi M, Johnson JT, Herberman RB, Whiteside TL. The absence of lymphoid suppressor cells in tumor-involved lymph nodes of patients with head and neck cancer. Cell Immunol.1990;130:446-458 https://doi.org/10.1016/0008-8749(90)90286-Z
  11. O'Malley BW Jr, Cope KA, Johnson CS, Schwartz MR. A new immunocompetent murine model for oral cancer. Arch Otolarylgol Head Neck Surg. 1997;123:20-24 https://doi.org/10.1001/archotol.1997.01900010022003
  12. Lee YS, Chung IS, Lee IR, Kim KH, Hong WS, Yun YS. Activation of multiple effector pathways of immune system by the antineoplastic immunostimulator acidic polysaccharide ginsan isolated from Panax ginseng. Anticancer Res. 1997;17:323-331
  13. Attele AS, Wu JA, Yuan CS. Ginseng pharmacology: Multiple constituents and multiple actions. Biochem Pharmacol. 1999;58:1685-1693 https://doi.org/10.1016/S0006-2952(99)00212-9
  14. Hashimoto W, Takeda K, Anzai R, Ogasawara K, Sakihara H, Sugiura K. Cytotoxic NK1.1 Ag+ alpha beta T cells with intermediate TCR induced in the liver of mice by IL-12. J Immunol. 1995; 154:4333-4340
  15. Anzai R, Hashimoto W, Sugiura K, Takahashi M, Satoh M, Kumagai K. Liver NK1.1+ CD4+ alpha beta T cells activated by IL- 12 as a major effector in inhibition of experimental tumor metastasis. J Immunol. 1996;156:3366-3373
  16. Herberman RB. Possible role of natural killer cells and other effector cells in immune surveillance against cancer. J Invest Dermatol.1984;83(Suppl):137-140 https://doi.org/10.1111/1523-1747.ep12282012