• 한국미생물·생명공학회지
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• 제49권3호
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• pp.413-424
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• 2021

식물과 근권미생물을 이용해 토양 오염물질을 제거하는 rhizoremediation의 효율을 높이기 위해서는 오염물질을 제거함과 동시에 식물 생장을 촉진시키는 미생물 자원 개발이 필요하다. 본 연구에서는 중금속 및 유류 복합 오염 토양에서 서식하고 있는 옥수수와 톨페스큐의 근권으로부터 중금속(구리, 카드뮴 및 납) 내성을 가진 근권세균을 순수분리하였고, 식물 생장 촉진능, 중금속 내성능 및 디젤 분해능을 정성적으로 평가하였다. 그 결과 중금속 내성, 식물 생장 촉진 활성 및 디젤 분해능을 가진 6종의 균주를 분리하였다. 옥수수 근권에서 분리한 CuM5와 CdM2 균주는 Cupriavidus sp.로 동정되었다. 톨페스큐 근권에서 분리한 CuT6, CdT2, CdT5 및 PbT3는 각각 Fulvimonas soli, Cupriavidus sp., Novosphingonium sp. 및 Bacillus sp.로 동정되었다. Cupriavidus sp. CuM5와 CdM2는 중금속 내성과 디젤 분해능은 상대적으로 낮았으나, 식물 생장 촉진능이 상대적으로 우수하였다. 6종 중에서 디젤 분해능이 가장 우수한 균주는 Cupriavidus sp. CdT2와 Bacillus sp. PbT3이었다. 특히, Bacillus sp. PbT3는 3종의 중금속에 대해 상대적으로 우수한 내성을 가졌고 식물 생장 촉진능도 우수하였다. 본 연구에서 분리한 근권세균은 유류와 중금속 복합 오염 토양을 정화시키며 식물 생장을 촉진시키는 새로운 미생물 자원으로 활용 가능하다.

• The Plant Pathology Journal
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• 제32권2호
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• pp.145-156
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• 2016

Fusarium graminearum Schwabe causes Fusarium head blight (FHB), a devastating disease that leads to extensive yield and quality loss of wheat and other cereal crops. Twelve isolates of F. graminearum were collected from naturally infected spikes of wheat from Assiut Egypt. These isolates were compared using SRAP. The results indicated distinct genetic groups exist within F. graminearum, and demonstrated that these groups have different biological properties, especially with respect to their pathogenicity on wheat. There were biologically significant differences between the groups; with group (B) isolates being more aggressive towards wheat than groups (A) and (C). Furthermore, Trichoderma harzianum (Rifai) and Bacillus subtilis (Ehrenberg) which isolated from wheat kernels were screened for antagonistic activity against F. graminearum. They significantly reduced the growth of F. graminearum colonies in culture. In order to gain insight into biological control effect in situ, highly antagonistic isolates of T. harzianum and B. subtilis were selected, based on their in vitro effectiveness, for greenhouse test. It was revealed that T. harzianum and B. subtilis significantly reduced FHB severity. The obtained results indicated that T. harzianum and B. subtilis are very effective biocontrol agents that offer potential benefit in FHB and should be harnessed for further biocontrol applications. The accurate analysis of genetic variation and studies of population structures have significant implications for understanding the genetic traits and disease control programs in wheat. This is the first known report of the distribution and genetic variation of F. graminearum on wheat spikes in Assiut Egypt.

• The Plant Pathology Journal
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• 제30권2호
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• pp.220-227
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• 2014

The GacS/GacA system in the root colonizer Pseudomonas chlororaphis O6 is a key regulator of many traits relevant to the biocontrol function of this bacterium. Proteomic analysis revealed 12 proteins were down-regulated in a gacS mutant of P. chlororaphis O6. These GacS-regulated proteins functioned in combating oxidative stress, cell signaling, biosynthesis of secondary metabolism, and secretion. The extent of regulation was shown by real-time RT-PCR to vary between the genes. Mutants of P. chlororaphis O6 were generated in two GacS-regulated genes, trpE, encoding a protein involved in tryptophan synthesis, and prnA, required for conversion of tryptophan to the antimicrobial compound, pyrrolitrin. Failure of the trpE mutant to induce systemic resistance in tobacco against a foliar pathogen causing soft rot, Pectobacterium carotovorum SCCI, correlated with reduced colonization of root surfaces implying an inadequate supply of tryptophan to support growth. Although colonization was not affected by mutation in the prnA gene, induction of systemic resistance was reduced, suggesting that pyrrolnitrin was an activator of plant resistance as well as an antifungal agent. Study of mutants in the other GacS-regulated proteins will indicate further the features required for biocontrol-activity in this rhizobacterium.

• Journal of Microbiology and Biotechnology
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• 제22권5호
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• pp.674-683
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• 2012

A new strain, SD12, was isolated from tannery waste polluted soil and identified as Pseudomonas aeruginosa on the basis of phenotypic traits and by comparison of 16S rRNA sequences. This bacterium exhibited broad-spectrum antagonistic activity against phytopathogenic fungi. The strain produced phosphatases, cellulases, proteases, pectinases, and HCN and also retained its ability to produce hydroxamate-type siderophore. A bioactive metabolite was isolated from P. aeruginosa SD12 and was characterized as 1-hydroxyphenazine ((1-OH-PHZ) by nuclear magnetic resonance (NMR) spectral analysis. The strain was used as a biocontrol agent against root rot and wilt disease of pyrethrum caused by Rhizoctonia solani. The stain is also reported to increase the growth and biomass of Plantago ovata. The purified compound, 1-hydroxyphenazine, also showed broad-spectrum antagonistic activity towards a range of phytopathogenic fungi, which is the first report of its kind.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.38-38
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• 2019

Among the 6500 accessions of the pepper germplasm which conserved at RDA Genebank, 319 accessions were investigated charateristics such as their growth habit, flowering and fruit shape in Capsicum frutescens. All accessions were characterized for their 14 qualitative and 16 quantitative characters. Results revealed that both qualitative and quantitative characters exhibited wide variation among the studied germplasm. The hypocotyl color, which investigated in juvenile stage, was divided in three types such as green, purple, and purple with green, and most of the accessions showed in purple. Flowering starts from 7 May, and most accessions were starting to flower from 27 May to 25 June. The fresh color of the mature pepper were red, yellow, orange, Etc. Fruit shape were mostly elongated, while exhibited ball shape and round shape were a few. The fresh weight of fruits ranged from 0.2 to 42.3, and the diameter of fruit showed from 4.2 to 45.1 mm, respectively. The phenotypic diversity exists in Capsicum frutescens. provide valuable information to improve agronomic traits in pepper breeding program.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.29-29
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• 2022

The fast-changing climatic conditions make plants to be vulnerable to many abiotic stresses. Drought stress is one of the limiting factors that affect pepper production in water deficient regions. It affects plant growth and development by altering physiological, morphological, and metabolic processes. Breeding drought tolerant varieties is one of the mitigation strategies to overcome the ever increasing drought disaster. Hence, screening of new drought tolerant pepper genotypes is essential. The current study was aimed to identify new drought tolerant genotypes among the collection of pepper genetic resources. In total, 70 pepper genotypes were screened for drought tolerance after exposure to drought stress condition. The pepper genotypes were classified as highly tolerant, intermediate, or severely sensitive to drought stress based on the phenotypic analysis. Consequently, 13 genotypes significantly exhibited higher recovery rate after drought stress and were classified as highly tolerant. Comparative analysis of morphological and physiological parameters and expression of drought responsive genes between tolerant and susceptible pepper genotypes will be presented and discussed. The identified tolerant genotypes will be useful resources for breeding drought tolerant pepper cultivars.

• Journal of Ecology and Environment
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• 제48권3호
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• pp.285-295
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• 2024

Although invasive alien species (IAS) have a negative impact on native ecosystems and reduce ecosystem services and productivity, the understanding of IAS at the population level is still lacking. Coreopsis lanceolata, a perennial plant native to North America, is expanding its invasive range, but there is limited research on the invasion risk of this species, and measures to control its spread are inadequate. Therefore, we collected findings from selected studies on C. lanceolata, examining its morphological and growth characteristics, reproductive traits, and invasion strategies, sourced from scientific databases using its scientific name as the keyword. Researchers have conducted extensive research on C. lanceolata, primarily focusing on the extraction of chemical compounds for anticancer and antioxidant effects and numerous studies on seed germination conditions in the field of ecology. Coreopsis lanceolata has a negative impact on plant ecosystems in Australia and Japan, and its high invasiveness is associated with high seed production, high seed viability and longevity, rapid reproduction through rhizomes, high surface coverage, and long flowering periods. Few studies have examined the invasive potential of C. lanceolate and management techniques to stop its spread, despite worries about the detrimental effects of invasion on plant ecosystems. Therefore, additional research on the invasion risk and management of C. lanceolata is necessary. This review offers a thorough analysis of C. lanceolata, serving as a scientific foundation for devising future ecosystem management strategies.

• 한국환경생태학회지
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• 제38권4호
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• pp.375-387
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• 2024

본 연구는 한국 특산식물이자 희귀식물인 물들메나무를 대상으로 분포 현황과 생육환경 특성, 종조성을 바탕으로 생태적 특성을 구명하였다. 물들메나무가 출현한 입지를 대상으로 생육환경 조건의 기재 및 식물사회학적 방법에 따라 식생조사가 수행되었으며, 출현종 양상과 환경변수의 상관관계 분석, 출현종의 식물 형질 분석을 수행하였다. 총 12개 지역, 28개 조사구에서 물들메나무 우점 입지 19개, 비우점 입지 9개가 확인되었으며, 총 155분류군이 출현하였다. 물들메나무 조사구의 생육환경은 한국 식생기후를 기준으로 대부분 온대북부 낙엽활엽수림대에 위치하여 한랭한 입지이며, 해발고도는 평균 859m, 암석노출도는 평균 60.4%, 토양노출도는 평균 24.7%, 경사도는 평균 18.7°로 나타났다. 출현종 중 상위 P-NCD(백분율기여도)에 해당하는 분류군은 대부분 계곡 식생에 출현하는 분류군으로 특기되었다. 환경변수의 상관관계 분석에서 해발고도가 가장 큰 상관관계를 보였으며, 그 다음은 암석노출도로 확인되었다. 물들메나무림의 동태는 교목수종에 해당하는 분류군 중 관목층과 초본층에서 물들메나무가 높은 P-NCD값을 보여 지속적으로 유지될 것으로 보인다. 현재 물들메나무가 출현하는 입지의 대부분은 국립공원, 도립공원, 군립공원 등의 보호구역내에 출현하여 인위적 교란요인이 비교적 적은 편이나, 일부 국가보호구역 미지정 지역의 경우 숲가꾸기 사업 또는 신규 등산로 개설로 인한 생육지 질의 쇠퇴 가능성이 있으므로, 이들 지역에 대하여 산림유전자원보호구역 설정과 같은 관리 방안이 제안되었다. 또한 물들메나무의 다양한 환경조건을 기반으로 잠재적 분포 가능 입지 파악 및 현지 외 보전 후보지역 설정을 위한 후속 조치 및 연구가 수행되어야 할 것이다.

• The Plant Pathology Journal
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• 제35권6호
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• pp.543-552
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• 2019

Fusarium asiaticum of the F. graminearum species complex causes head blight in small-grain cereals. The nivalenol (NIV) chemotypes of F. asiaticum is more common than the deoxynivalenol (DON) chemotypes of F. asiaticum or F. graminearum in Korea. To understand the prevalence of F. asiaticum-NIV in Korean cereals, we characterized the biological traits of 80 cereal isolates of F. asiaticum producing NIV or 3-acetyl-deoxynivalenol (3-ADON), and 54 F. graminearum with 3-ADON or 15-acetyl-deoxynivalenol (15-ADON). There was no significant difference in mycelial growth between the chemotypes, but F. asiaticum isolates grew approximately 30% faster than F. graminearum isolates on potato dextrose agar. Sexual and asexual reproduction capacities differed markedly between the two species. Both chemotypes of F. graminearum (3-ADON and 15-ADON) produced significantly higher numbers of perithecia and conidia than F. asiaticum-NIV. The highest level of mycotoxins (sum of trichothecenes and zearalenone) was produced by F. graminearum-3-ADON on rice medium, followed by F. graminearum-15-ADON, F. asiaticum-3-ADON, and F. asiaticum-NIV. Zearalenone levels were correlated with DON levels in some chemotypes, but not with NIV levels. Disease assessment on barley, maize, rice, and wheat revealed that both F. asiaticum and F. graminearum isolates were virulent toward all crops tested. However, there is a tendency that virulence levels of F. asiaticum-NIV isolates on rice were higher than those of F. graminearum isolates. Taken together, the phenotypic traits found among the Korean F. asiaticum-NIV isolates suggest an association with their host adaptation to certain environments in Korea.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.52-52
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• 2020

병아리콩은 두류 중 콩, 땅콩, 강낭콩, 완두 다음으로 중요한 작물로 chickpea, garbanzo bean 이라고도 불리며, 원산지는 터키 동남부와 시리아로 알려져 있다. 병아리콩은 전세계 50개국 이상에서 재배되며, 인도에서 전세계 생산량의 66%가 재배되고 있다. 병아리콩은 Desi (microsperma)와 Kabuli (macrosperma)의 두가지 형태로 구분된다, Desi형은 꽃색이 분홍색, 종피가 두껍고 종피색이 유색이 특징, Kabuli형은 백색꽃에, 백색이나 미색 종피 양머리 종자형태를 보인다. Desi 형은 아시아와 아프리카, Kabuli 형은 서아시아, 북아프리카, 남미와 유럽 등지에서 주로 재배된다. 본 연구에서는 인도, 미얀마, 우즈벡, 조지아, 불가리아, 우크라이나 등 6개국에서 수집한 자원의 농업형질을 평가하고 비교하여 활용을 위한 정보를 제공하고자 수행하였다. 꽃색의 분포는 분홍색이 백색보다 2배 많았으며 조지아, 인도와 미얀마 수집 자원은 80% 이상이 분홍색, 우크라이나, 우즈베키스탄에서는 백색이 최소 75%이상으로 조사되었다. 소엽의 크기는 종자의 크기와 밀접한 관련이 있으며 인도와 미얀마, 불가리아에서는 소립종, 조지아, 우크라이나에서는 중립종의 비율이 높았다. 개화기는 6월 상중순에 집중되었으며 인도와 미얀마 수집자원의 개화기가 6월 상순 이전으로 빠르고 우크라이나 자원이 6월 하순과 7월 상순으로 늦은 경향을 보였다. 올해는 6월 중순부터 지속된 강우로 개화 후 정상적인 결실이 어려웠으나 6월 하순부터 성숙하기 시작하였고, 인도와 미얀마 수집자원은 주로 7월 상순에 나머지 수집 국가들은 7월 하순 이후에 성숙하였다. 올해의 기상이변으로 보다 정확한 평가를 위하여 차년도에 재평가를 수행하고자 하나 올해의 양적, 질적 형질의 경향치를 이용하여 수집국간의 농업형질을 비교하고, 식품소재의 다양화 다변화 추세에 맞추어 도입작물 활용을 위한 기초자료로 제공하고자 한다.