• Title/Summary/Keyword: Plant foundation

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Analysis of Genes Activated by Salt and ER Stress in bZIP17 and bZIP28 Gene Transgenic Potato Plants

  • Kim, Kyung Hwa;Choi, Man Soo;Chun, Jae Buhm;Jin, Mi Na;Jeong, Nam Hee;Kim, Dool Yi
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2018.10a
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    • pp.179-179
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    • 2018
  • Potato (Solanum tubersosum L.) is susceptible to various environmental stresses such as salt, high temperature, and drought. Especially, potato tuber growth is greatly affected by drought that causes not only yield reduction but also loss of tuber quality. Since unpredictable global weather changes cause more severe and frequent water limiting conditions, improvement of potato drought tolerance can minimize such adverse effects under drought and can impact on sustainable potato production. Genetic engineering can be utilized to improve potato drought tolerance, but such approaches using endogenous potato genes have rarely been applied. We were obtained AtbZIP28 gene transgenic potato plants. It is identified transcript levels at various stress conditions, polyethylene glycol (PEG), NaCl, (ABA). Also, For identification to regulate ER stress response genes in AtbZIP28 gene transgenic potato plant, we screened seven potato genes from RNA-seq analysis under TM treatment. Five and two genes were up- and down-regulated by TM, respectively. Their expression patterns were re-examined at stress agents known to elicit TM, DTT, DMSO and salt stress.

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Status of Molecular Biotechnology Research Based on Tissue Culture of Soybean (콩 조직배양 기술에 기반한 생명공학 연구 동향)

  • Seo, Mi-Suk;Cho, Chuloh;Choi, Man-Soo;Chun, JaeBuhm;Jin, Mina;Kim, Dool-Yi
    • Korean Journal of Plant Resources
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    • v.33 no.5
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    • pp.536-549
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    • 2020
  • Soybean (Glycine max (L.) Merrill) is one of the most important crops of the world. With the completion of the soybean genome sequence, the Korean soybean core collection consisted of 430 accessions with genetic and phenotypic diversity was constructed in recent year. The availability of genome sequences and core collection will result in the crop improvement by molecular breeding using the various accessions and genome editing approaches. Efficient tissue culture techniques, such as haploid production, protoplast culture and plant regeneration from various organs are essential for the successful molecular biological approach and crop improvement. However, soybean is still considered to be recalcitrant in tissue culture because of the low frequency of regeneration and limitation of available responsive cultivars. In this study, we discuss the recent studies of tissue culture technology and methodology for efficient tissue culture to genetic improvement and application of molecular biotechnology in soybean.

First Report of Bacterial Wilt by Ralstonia pseudosolanacearum on Peanut in Korea (Ralstonia pseudosolanacearum에 의한 땅콩 풋마름병 발생 보고)

  • Choi, Soo Yeon;Kim, Nam Goo;Kim, Sang-Min;Lee, Bong Choon
    • Research in Plant Disease
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    • v.28 no.1
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    • pp.54-56
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    • 2022
  • A peanut plant showing wilt and browned symptom was found in the field of Gochang, Korea, in July 2021. The symptomatic peanut plant was collected from the field and isolation of the pathogen caused the wilt symptom was performed using the collected sample on TZC media. The dominated colony on media was isolated colony on media was isolated and subcultured of purification. The pure cultured bacteria was identified as Ralstonia solanacearum by sequencing of 16S rRNA gene. Multiplex polymerase chain reaction using phylotype-specific primer set identified isolate as phylotype I (R. pseudosolanacearum). Phylogenetic tree was constructed based on 16S rRNA sequence and it was closed with R. pseudosolanacearum. Pathogenicity of the isolates was assessed by soil drenching inoculation on 4-week-old peanut plant. The wilt symptom was successfully reproduced by inoculation of the isolates after 14 days. This is first report of bacterial wilt caused by R. pseudosolanacearum on peanut in Korea.

Soil-structure interaction analysis for the offshore wind tower with bucket foundation (버켓기초를 가진 해상풍력타워의 지반-구조물 상호작용해석)

  • Lee, Gyehee;Kim, Sejeong;Phu, Tranduc
    • Journal of the Society of Disaster Information
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    • v.10 no.2
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    • pp.244-252
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    • 2014
  • In this study, seismic responses of the offshore wind tower supported by bucket foundation are analyzed in consideration of soil-structure interaction. The program SASSI is used as analyzing tool and an artificial seismic input for soft soil is used as input motion. The H/R ratio of bucket, the stiffness of bucket foundation and the soil stiffness are considered as parameters and its effects are estimated. The responses of structure are obtained at the base and the nacell. As results, the effects of H/R ratio, the stiffness of bucket and the stiffness of site are generally denoted different response tendency at the base and the nacell. However, these whole responses of the base and the nacell are much lager than that of rock site. Therefore, the consideration of this phemomia affect to the response of offshore wind tower with bucket foundation largely.

Operating Vibration Reduction Effect Evaluation of EDG at the NPP Site (원자력발전소 비상디젤발전기의 가동중 진동저감 효과)

  • Kim, Min-Kyu;Choun, Young-Sun
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2006.11a
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    • pp.111-118
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    • 2006
  • The Emergency Diesel Generator(EDG) is a very important piece of equipment for the safety of a Nuclear Power Plant(NPP). In this study, the operating vibration or three kinds or EDG system was measured. The target EDG systems art Yonggwang 5 unit, Ulchin 2 unit and Ulchin 3 unit. The Yonggwang 5 and Ulchin 3 unit EDG system is the same type but the foundation systems are different. One is an anchor bolt type and the other is a spring and viscous-damper type. The purpose of this measurement is for a verification of the vibration isolation effect according to the foundation system. As a result. it can he said that the spring and viscous damper system of the EDG performed well for the vibration isolation.

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Development of Seismic Stability Evaluation Technology for Rock Foundation of Nuclear Power Plant (원전 기초지반의 내진안정성 평가절차 개발)

  • Hwang, Seong-Chun;Jang, Jung-Bum;Lee, Dae-Su;Kim, Yun-Chil
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2005.03a
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    • pp.74-81
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    • 2005
  • The purpose of this study is to suggest a proper analysis model that can evaluate seismic stability for local rock foundation of nuclear power plant. Sliding Analysis, Pseudo-static Analysis and Danamic Analysis methods are used for analysing NPP rock foundation with the conditions like acting directions of input earthquake, boundary conditions, width and depth of analysing model, and modeling methods of weakness fault zones. As the results of study, Pseudo-static Analysis for lateral roller and dynamic analysis for transfer boundary condition showed good results, and analysing ranges of width and depth were 5 times of structure width and over 2 times of structure depth.

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A Study on the Development of a Ultra-Strength Precast Concrete Bearing Concrete Bearing Plate (초고강도 ($\acute{f}_{C91}$= 950kg/$\textrm{cm}^2$) P.C Bearing Plate 개발에 관한 연구)

  • 소현창;정병욱;김재우;문성규
    • Proceedings of the Korea Concrete Institute Conference
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    • 1997.04a
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    • pp.643-648
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    • 1997
  • P.C Bearing Plate method, corresponding to the existing steel plate build-up method, is developed by the very first in domestic and is applied to the foundation in the HYUNDAI building at Kang-Nam. P. C Bearing Plate produced in ourself P.C plant can stand against vertical load of 7,000ton obtaining allowable force of soil. It is possible to minmize cost expediting, do site assembling and omit unnecessary excavation work by plant prefabrication of foundation member. The purpose of this paper is to study the optimum mixing design of Ultra-high strength concrete ($\acute{f}_{C91}$= 950kg/$\textrm{cm}^2$), crack control through measuring the heat of hydration, mock up test for the optimum curing method. As mentioned above, developing the Ultra-high strength Precast Concrete Bearing Plate set up successfully in the site foundation work of the HYUNDAI Building at Kang-Nam.

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Experimental Performance Evaluation of Complex Behavior Connector by Scaled Model (축소모형에 의한 복합거동 연결체의 실험적 성능 평가)

  • Kim, Kisung;Kang, Hyounhoi;Park, Jeongjun
    • Journal of the Society of Disaster Information
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    • v.13 no.2
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    • pp.130-138
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    • 2017
  • The connector of the complex behavior is to connect the individual piles of the pile to the lower foundation of the oil sand plant where the floating foundation is used. In this study, to verify the shape of a connector of the complex behavior for applying the advantages of existing group pile and piled raft foundation to an oil sand plant, a scaled model was constructed to measure the behavior of the load.

Spread Patterns of Thermal Effluent Discharged From Young-Kwang Nuclear Power Plant Using Remote Sensing Data

  • Han J. G.;Yeon Y. K.;Chi K. H.
    • Proceedings of the KSRS Conference
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    • 2004.10a
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    • pp.331-335
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    • 2004
  • This study is focused to analyze the movement of thermal effluent dischargeed from nuclear power plant by season, ebb and flow, and before and after foundation of tide embankment using thermal infrared band image of 28 scenes observed from Landsat from 1987 to 2004, which is the early stage of operation of young-kwang nuclear power plant. In diffusion of thermal effluent discharge by seasons, spring and summer is spreading further than autumn and winter. It is considered to distribute widely mixed with thermal effluent discharge and hot water, which is distributed naturally along the seaside. It is known the fact that tidal currents control the direction of diffusion of thermal effluent discharge by the change of ebb and flow. Namely, it is distributed widely on the Southwest direction along the seaside by tidal currents when ebb and, it is moved widely on the Northeast direction along the seaside by tidal current when flood. However, in the early stage of flood current, the mainstream of thermal effluent discharge is spread on Southwest direction and, the direction is changed on North­east way when the latter period of flood current. Similarly, in the early stage of ebb current, the mainstream of thermal effluent discharge is spread on Northeast direction and, the direction is changed on Southwest direction when the latter period of ebb current. As the result of comparing to the diffusion pattern of thermal effluent discharge before and after the foundation of seawall, discharged thermal effluent from the drain of plant by the foundation of dike is shown as curved circle pattern on Northeast to West direction from the ending portion of the seawall.

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