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http://dx.doi.org/10.5423/RPD.2022.28.1.54

First Report of Bacterial Wilt by Ralstonia pseudosolanacearum on Peanut in Korea  

Choi, Soo Yeon (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration)
Kim, Nam Goo (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration)
Kim, Sang-Min (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration)
Lee, Bong Choon (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration)
Publication Information
Research in Plant Disease / v.28, no.1, 2022 , pp. 54-56 More about this Journal
Abstract
A peanut plant showing wilt and browned symptom was found in the field of Gochang, Korea, in July 2021. The symptomatic peanut plant was collected from the field and isolation of the pathogen caused the wilt symptom was performed using the collected sample on TZC media. The dominated colony on media was isolated colony on media was isolated and subcultured of purification. The pure cultured bacteria was identified as Ralstonia solanacearum by sequencing of 16S rRNA gene. Multiplex polymerase chain reaction using phylotype-specific primer set identified isolate as phylotype I (R. pseudosolanacearum). Phylogenetic tree was constructed based on 16S rRNA sequence and it was closed with R. pseudosolanacearum. Pathogenicity of the isolates was assessed by soil drenching inoculation on 4-week-old peanut plant. The wilt symptom was successfully reproduced by inoculation of the isolates after 14 days. This is first report of bacterial wilt caused by R. pseudosolanacearum on peanut in Korea.
Keywords
Bacterial wilt; Peanut; Ralstonia pseudosolanacearum;
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Times Cited By KSCI : 3  (Citation Analysis)
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