• Title/Summary/Keyword: Plant disease and insect

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Detection of Rice Stripe Virus using RT-PCR (RT-PCR에 의한 벼 줄무늬잎마름병 정밀진단)

  • Lee, Bong-Choon;Hong, Yeon-Kyu;Kwak, Do-Yeon;Oh, Byeong-Geun;Park, Sung-Tae;Kim, Soon-Chul
    • Research in Plant Disease
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    • v.10 no.1
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    • pp.30-33
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    • 2004
  • Until now, occurrence of rice stripe virus (RSV) is limitted in southern part of Korea. However, recently the occurrence of RSV is increased and spreaded in central part of Korea including Chungcheong and Kyonggi province. It is very difficult to distinguish RSV symptoms on virus symptom physiological damage of rice. We detected RSV viral RNA from infected rice and its insect vector Laodelphax striatellus using specific primer of RSV-polymerase and coat protein gene with reverse transcription (RT)-PCR. The result of RT-PCR, we observed specific band including RSV-polymerase (1,,023 bp) and CP (969 bp) in both host of rice and insect vector.

Disease Resistance-Based Management of Alternaria Black Spot in Cruciferous Crops (병 저항성 기반 십자화과 작물의 검은무늬병 관리)

  • Young Hee Lee;Su Min Kim;Seoung Bin Lee;Sang Hee Kim;Byung-Wook Yun;Jeum Kyu Hong
    • Research in Plant Disease
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    • v.29 no.4
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    • pp.363-376
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    • 2023
  • Alternaria black spots or blights in cruciferous crops have been devastating diseases worldwide and led to economic losses in broccoli, Chinese cabbage, kale, radish, rapeseed, etc. These diseases are caused by different Alternaria spp., including A. brassicae, A. brassicicola and A. raphani transmitted from infected seeds or insect vectors. Efforts to excavate disease resistance traits of cruciferous crops against Alternaria black spots or blights have been demonstrated. Genetic resource of disease resistance was investigated in the wild relatives of cruciferous crops, and different cultivars were screened under different inoculation conditions. Development of the disease-resistant lines against Alternaria black spots or blights was also tried via genetic transformation of the cruciferous crops using diverse plant defence-associated genes. Plant immunity activated by pre-treatment with chemicals, i. e. β-amino-n-butyric acid and melatonin, was suggested for reducing Alternaria black spots or blights in cruciferous crops. The disease resistance traits have also been evaluated in model plant Arabidopsis originating from different habitats. Various plant immunity-related mutants showing different disease responses from wild-type Arabidopsis provided valuable information for managing Alternaria black spots or blights in cruciferous crops. In particular, redox regulation and antioxidant responses altered in the Alternaria-infected mutants were discussed in this review.

Development and evaluation of a model for management of plant pests in organic cucumber cultivation

  • Ko, S.J.;Kang, B.R.;Kim, D.I.;Choi, D.S.;Kim, S.G.;Kim, H.K.;Kim, H.J.;Choi, K.J.;Kim, Y.C.
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.263-266
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    • 2011
  • Crop protection strategies in organic horticulture aim to prevent insect pest and plant disease problems through utilization of non-chemical based control means. In order to develop a model for management of plant diseases and insects in organic cucumber cultivation, we compared efficacies between chemical pesticide spraying system and biological control means in semi-forcing and retarding cucumber cultivation during 2005 and 2006. Conventional chemical spray program using various chemical pesticides was applied 5 - 10 days intervals, while two different non-chemical pesticide application programs using two formulated biopesticides Topseed$^{TM}$ and Q-fect$^{TM}$, Suncho$^{TM}$, and Sangsungje$^{TM}$ (biocontrol agents 1) and using egg-yolk and cooking oil(EYCO), Bordeaux mixture, Suncho$^{TM}$, and Sangsungje$^{TM}$ (biocontrol agents 2) were applied 5 - 7 days intervals during entire cucumber cultivation period. Efficacy of both biocontrol agents programs was effective to comparable to conventional chemical pesitice spray program to control plant diseases such as powdery mildew and downy mildew as well as insect pests such as aphids and thrips which are known as major threats in cucumber organic cultivation. In this study, we established and evaluated an effective and economic crop protection strategy using various biological resources can be used to control plant diseases and pests simultaneously in organic cucumber cultivation field.

A Review of the List of Plant Diseases in Korea and the Names of Korean Tree Diseases (한국식물병명목록과 우리나라 나무병 이름에 대한 소고)

  • Byeongjin Cha;Sang-Tae Seo;Sang-sup Han
    • Research in Plant Disease
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    • v.30 no.1
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    • pp.1-12
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    • 2024
  • Since the List of Plant Diseases in Korea (DisList) was first published in 1986, the 6th edition appeared 36 years later. In 2023, the 6.1 edition, a revised and improved version of the 6th edition, was released to the public on the web free of charge. The contents of DisList increased, with the number of hosts increasing from 437 taxa to 1,420 taxa and the number of disease species increasing from 1,539 to 6,680. Among these, tree diseases are 3,586 species and their hosts include 504 taxa, providing much help to experts who need them. Meanwhile, the importance of accurate disease names continues to grow with the legalization of tree care, but many disease names are still inappropriate or misused, causing confusion. Disease names that do not follow the naming regulations are still registered, and even if the same pathogen infects hosts of the same taxa, the disease names are given differently, and there are many disease names that do not indicate the characteristics of the disease. Also, there are diseases reported without Korean names. In order to make DisList better, the review committee for disease names should establish the regulations to review and register disease names, and establish a system to review new disease names before publishing papers.

Studies on the Some Aspect of Small Brown Planthopper Transmission of Rice stripe tenuivirus (벼줄무늬잎마름병을 매개하는 애멸구의 전염생태)

  • Park, Jin-Woo;Lee, Min-Ho;Lee, Key-Woon
    • The Korean Journal of Pesticide Science
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    • v.15 no.4
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    • pp.490-494
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    • 2011
  • Rice stripe virus (RSV) has been the main viral disease of rice plant in western coastal region of Korea since 2000. The control of the vector insect, small brown planthopper (Laodelphax striatellus), is the most effective management method of the persistently-transmitted viral disease. Thus, ecological study between RSV and the vector insect was needed and investigated in order to make effective control plan, especially about study on the feeding and transmission of the virus by the vector insect. Each larval stage of vector insect differed in vector competence; larvae over 4th stage were shown as higher transmission after feeding on RSV-infected rice plant. These 4th and 5th larvae had higher transmission rates, 69.2% and 67.9% respectively, than 44.8% of the adult stage. The vector competence, however, was changed according to temperature; the highest transmission rate was 93.3% on $30^{\circ}C$ in comparison to 70.6% on $25^{\circ}C$ and 43.8% on $20^{\circ}C$.

Control Efficacy of Fungicide Injection on Oak Wilt in the Field (살균제 나무주사를 이용한 참나무 시들음병 방제 효과)

  • Son, Su-Yeon;Seo, Sang-Tae;Park, Ji-Hyun
    • Research in Plant Disease
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    • v.20 no.4
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    • pp.295-298
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    • 2014
  • Oak wilt caused by Raffaelea quercus-mongolicae was first noticed in South Korea in 2004 and, ever since, its distribution and damage have been increasing. To screen a fungicide effective for oak wilt control by tree injection, laboratory and field experiments were conducted. Ten fungicides and one antibiotic were examined in vivo for their effectiveness in restricting the growth of R. quercus-mongolicae and R. quercivora (Japanese oak wilt pathogen) isolates. To the Korean isolates of R. quercus-mongolicae, chlorothalonil showed the highest fungicidal effects, followed by benomyl and propiconazole. To the Japanese one, propiconazole was highest in the fungicidal effectiveness, followed by benomyl and bitertanol. Propiconazole was selected for field-testing of its control efficacy because it showed good fungicidal effects in vitro and systemic activity. The control efficacy in the field was 87.5% in the first year of injection and 66.7% in the second year, indicating the fungicidal effects last at least over one year.

Seasonal Occurrence of Diseases and Insect Pests in Small Type Watermelon and Effect of Organic Materials on Powdery Mildew Control (소과종 수박의 병해충 발생소장과 유기농업자재를 이용한 흰가루병 방제효과)

  • Han, Jong-Woo;Jeong, Taek-Goo;Kim, Young-Sang;Kim, Tae-Il;Noh, Sol-Ji;Kim, Ik-Jei
    • Korean Journal of Organic Agriculture
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    • v.25 no.2
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    • pp.419-429
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    • 2017
  • We investigated the occurrence pattern of diseases and insect pests and disease control efficacy of organic materials against watermelon powdery mildew in small type watermelon in Eumseong, Chungcheongbuk-do, 2015. The result of this study, the small type watermelon was damaged by diseases and pests such as Didymella bryoniae, Podosphaera xanthii, Aphis gossypii, Tetranychus urticae, thrips, Spodoptera exigua and Spodoptera litura. Among them, the occurrence of P. xanthii, T. urticae and thrips was high. Diseased leaf rate by P. xanthii, a casual agent of powdery mildew, was 27~99.3% in three small type watermelon cultivars in the middle of June. The number of T. urticae per leaf was high from 79.9 to 111 in three small type watermelon cultivars in the middle of June. It showed high number of thrips captured by yellow and blue sticky trap. Highest numbers of yellow sticky trap (407) and of blue sticky trap (774) were detected in the middle and first of June, respectively. The disease control efficacy of mayonnaise, oleic acid and three organic materials against powdery mildew of small type watermelon in fields was evaluated. As thre results, the symptoms of plant disease were effectively reduced by over 60% in the treatments of materials such as sodium bicarbonate 80%, mayonnaise and the extract of Rheum palmatum 1%. The highest control efficacy was 83% in the treatment of sodium bicarbonate 80%. From this study, we had a information of the occurrence pattern of diseases and insect pests in small type watermelon and the treatment of material containing sodium bicarbonate 80% was very effective for controlling against powdery mildew.

Foreign Case Analysis and Implications for Risk Assessment of Industrial Insects (산업곤충 위해성 평가를 위한 국외사례 분석 및 시사점)

  • Yoo, Mi-Na;Jang, Ki-Jung;Hwang, Jung-Hoon;Park, Yong-Ha;Ro, Tae Ho
    • Journal of Environmental Policy
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    • v.13 no.4
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    • pp.135-159
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    • 2014
  • Industrial insect is defined as the insect utilized in industries that creates added value. Most of the industrial insects used in Korea are exotic species that are introduced through artificial means. Despite the rapid expansion of market for industrial insects, the system for risk assessment of industrial insects is not being adequately conducted. Although Korea carries out a risk assessment for the species designated as disease and insect pest by Animal and Plant Quarantine Agency, far too little consideration is being given to overall ecosystem, as the control system is covered in the Plant Quarantine Law. To solve this problem, we analyzed the Korean risk assessment system and looked at systems in other countries. The results show that it is essential for stakeholders to reach an agreement to set up fundamental directions for the system. Unless the integration system of taxonomical and ecological information is prepared, the ecological risk assessment should be conservative to protect ecosystems and should also follow the precautionary principle. It also requires cooperation among the ministries. In addition, the results indicated that a differentiation between risk assessment and screening is urgent. Several solutions such as setting up clear objectives in both assessment and screening stages, target species, steering organization and assessment criteria assessment systems from were proposed as practical institutional strategies. Among many foreign countries the assessment system from Ireland equally considers various factors such as economical, ecological safety and management aspects, It is also based on precautionary principle to fulfil its original purpose. It was suggested that the Ireland system would be the best reference that can be modified and applied into the Korean system by considering distinct characteristics of the industrial insects.

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Viruliferous Rate of the Small Brown Planthopper in the Maize Field Infected with Rice black-streaked dwarf virus (옥수수 재배지의 벼검은줄오갈병 매개충인 애멸구의 보독충율)

  • Lee, Bong-Choon;Jung, Ji-Hun;Kim, Jung-In;Yoon, Young-Nam;Hong, Sung-Jun;Kwak, Do-Yeon;Hong, Yeon-Kyu;Kang, Hwang-Won;Lee, Key-Woon
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.226-228
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    • 2008
  • The Rice black-streaked dwarf virus (RBSDV) infected 99-100% of a $1000\;m^2$-maize field in Mungyeong City in 2007. Adjacent to the area is a Persimmon orchard where barley crops were grown under the trees as green manure crops and for soil amendments. The barley acted as winter host to the small brown plant hoppers (SBPH) enabling the insects to survive and pass the winter season. The existence of RBSDV was detected and confirmed by RT-PCR using S9 specific primer. Samples of the insect vector SBPH were collected in the area on May 3, June 7 and, August 4 and the results of the RT-PCR analysis revealed viruliferous insect rates of 2.9, 4.8, and 4.4%, respectively. These observed viruliferous insect rates were similar with those detected in RBSDV infected rice fields.

Diagnosis of Cylindrocarpon destructans Using Enzyme-Linked Immunosorbent Assay

  • Li, Taiying;Ji, Sungyeon;Jung, Boknam;Kim, Bo Yeon;Lee, Kwang Sik;Seo, Mun Won;Lee, Sung Woo;Lee, Jungkwan;Lee, Seung-Ho
    • Research in Plant Disease
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    • v.25 no.3
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    • pp.131-135
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    • 2019
  • Cylindrocarpon destructans causes ginseng root rot and produces radicicol that has an antifungal effect. In this study, we developed a method to detect this fungus using enzyme-linked immunosorbent assay (ELISA). Secreted proteins of C. destructans were used as antigens to obtain C. destructans-specific IgG from mouse. Out of 318 monoclonal antibodies generated from mouse, two antibodies (Cd7-2-2 and Cd7-2-10) showed highest specificity and sensitivity. Indirect ELISA using both antigens successfully detected C. destructans in soils, but direct ELISA using IgG conjugated with horseradish peroxidase failed to detect antigens in soils. The indirect ELISA developed here can efficiently detect the fungus and help manage ginseng root rot disease in fields.