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http://dx.doi.org/10.5423/RPD.2019.25.3.131

Diagnosis of Cylindrocarpon destructans Using Enzyme-Linked Immunosorbent Assay  

Li, Taiying (Department of Applied Biology, Dong-A University)
Ji, Sungyeon (Department of Applied Biology, Dong-A University)
Jung, Boknam (Forest Insect Pests and Diseases Division, National Institute of Forest Science)
Kim, Bo Yeon (Department of Applied Biology, Dong-A University)
Lee, Kwang Sik (Department of Applied Biology, Dong-A University)
Seo, Mun Won (Ginseng Research Division, National Institute of Horticultural and Herbal Science)
Lee, Sung Woo (Ginseng Research Division, National Institute of Horticultural and Herbal Science)
Lee, Jungkwan (Department of Applied Biology, Dong-A University)
Lee, Seung-Ho (Ginseng Research Division, National Institute of Horticultural and Herbal Science)
Publication Information
Research in Plant Disease / v.25, no.3, 2019 , pp. 131-135 More about this Journal
Abstract
Cylindrocarpon destructans causes ginseng root rot and produces radicicol that has an antifungal effect. In this study, we developed a method to detect this fungus using enzyme-linked immunosorbent assay (ELISA). Secreted proteins of C. destructans were used as antigens to obtain C. destructans-specific IgG from mouse. Out of 318 monoclonal antibodies generated from mouse, two antibodies (Cd7-2-2 and Cd7-2-10) showed highest specificity and sensitivity. Indirect ELISA using both antigens successfully detected C. destructans in soils, but direct ELISA using IgG conjugated with horseradish peroxidase failed to detect antigens in soils. The indirect ELISA developed here can efficiently detect the fungus and help manage ginseng root rot disease in fields.
Keywords
Cylindrocarpon destructans; Diagnosis; Enzyme-linked immunosorbent assay; Fusarium solani; Ginseng root rot;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
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