• Korean Journal of Soil Science and Fertilizer
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• v.37 no.4
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• pp.235-244
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• 2004

In this study, we compared the levels of methylotrophic bacterial community diversity in the leaf samples of 19 rice cultivars collected from three regions of Korea. Nineteen pink pigmented isolates showing characteristic growth on methanol were obtained. Physiological and biochemical characters of each isolate were examined according to methods described in Bergey's Manual of Systematic Bacteriology. When phylotypes were defined by performing numerical analysis of 37 characteristics, four distinct clusters were formed. The two reference strains, Methylobacterium extorquens AM1 and Methylobacterium fujisawaense KACC10744 were found to group under cluster IV and cluster III respectively. Cluster I diverged on the basis of nitrate reduction and four isolates showed tolerance upto 0.5 M NaCl concentrations. Two strains in cluster I and III were found to possess methane utilizing properties. Most of the isolates in all the four clusters utilized monosaccharides, disaccharide and polyols as carbon source. When the isolates were subjected for indole-3-acetic acid (IAA) analysis in the presence of L-tryptophan, only 8 isolates exhibited IAA production. In addition, the nitrogen source in the medium was found to influence the IAA production. Addition of $(NH_4)_2SO_4$ in the medium led to a 2 to 30 fold increase in the indole synthesis. However, $KNO_3$, $NH_4NO_3$ and $NH_4Cl$ substitution did not significantly stimulate the synthesis of IAA in the growth medium. Result of gnotobiotic root elongation assay significantly increased roots and shoots lengths, and number of lateral roots, which is mediated by IAA production in the culture medium. The rice seedlings primary roots from seeds treated with methylotrophic isolates were on average 27 to 56% longer than the roots from seeds treated with the uninoculated seeds. In addition, application of different high concentrations of authentic IAA ($400g\;mL^{-1}$) to roots of rice seedlings inhibited root growth. However, the IAA concentration from 10 to $200g\;mL^{-1}$, IAA promoted root growth of rice seedlings. These results suggest that bacterial IAA plays a major role in the development of the host plant root system.

• Applied Biological Chemistry
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• v.37 no.3
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• pp.182-188
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• 1994

Similar to the $^{42}K$ uptake, $^{86}Rb$ uptake by the roots of Hordeum distichum grown in the hydroponic culture was negatively correlated with the concentration of K supplied previously, showing that $^{86}Rb$ can be used for the K-bioassay. $^{86}Rb$ having longer half life (18.86 day) than $^{42}K$ (12.36 hr) allowed the use of larger number of root samples. $^{86}Rb$ uptake of 3 years old Citrus unshiu Marc. grown in water culture decreased drastically with the increase of K concentration of the culture solution, thus demonstrating that the nutrition status of K for citrus trees can be diagnosed by K-bioassay using $^{86}Rb$ tracer. $^{86}Rb$ uptake by the excised roots of Hordeum distichum grown in the pot with different K fertilizations was well correlated with the exchangeable K in soil. The amount of exchangeable K in soil for the optimal plant growth can be determined by its relationship. $^{42}K$ and $^{86}Rb-uptake$ by the Hordeum distichum roots were markedly inhibited by $5{\times}10^{-3}\; M$ KCN in the bioassay solution, indicating that uptake is energy-dependent. There was no significant relationship between K content in citrus leaves and K concentration in the water-culture medium. It is concluded that K-bioassay is a potentially useful tool for determining of K requirement in citrus trees.

• Journal of Bio-Environment Control
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• v.27 no.1
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• pp.1-6
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• 2018

Also, t-cincreaseisdecreasein order In hydroponics, the accumulation of inorganic ions in the root zone are closely related to the irrigation volume. Therefore, the effects of irrigation volume on the growth and yield of tomatoes are very signigicant. This study was conducted to investigate the effect of irrigation volume on inorganic ions of root zone in hydroponic culture using coir substrate. The irrigation volume was adjusted to 4 levels depending on the integrated solar radiation for each growth period. The drainage ratio was calculated by daily amount of irrigation and drainage. The higher irrigation volume is, drainage ratio and water absorption tended to increase. But, the water absorption in the treatment of high irrigation volume was decreased in February and March compared to the treatment of medium high irrigation volume. By calculating monthly average irrigation volume and the drainage ratio, 120 to 1$40J/cm^2$ in January, 100 to $120J/cm^2$ in February, 80 to $100J/cm^2$ in March, 70 to $90J/cm^2$ in April and 60 to $75J/cm^2$ in May was detected as appropriate irrigation volume ranges which drainage ratio was 20-30%. The higher irrigation volume, the lower the concentration of ions decrease, which could prevent the accumulation of nutrients in the root zone. However, due to the characteristics of the coir substrate that absorbs ions, concentration of ions was significantly high when the drainage ratio was 20-30%. However, concentrations of P and K were sometimes lower in the drainage than that of irrigation water regardless of the treatment. Mg and S were the most highly accumulated ions even in the treatment of high irrigation volume. In low radiation season, there was no difference in the ion concentration in the drainage depending on the irrigation volume. In high radiation season, the lower irrigation volume, resulted to the higher ion concentration in the drainage. After March, it was difficult to prevent the increase of ions concetration in the drainage by only adjusting irrigation volume. Thus, it is necessary to decrease the EC of irrigation solution to prevent the accumulation of nutrients in the root zone.

• Journal of Korean Society of Forest Science
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• v.78 no.4
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• pp.333-344
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• 1989

The objectives of this study were to investigate the growth of Gyrophora esculanta and to establish a method of tissue culture of the plant. The results obtained were as follows : 1. The Gyrophora esculanta was found growing mostly on the rock slopes of 722 m to 1915 min elevation on mountains in Korea. 2. Trees growing in the vicinity of the G. esculanta were mainly Quercus spp., Pinus thunbergii, Acer spp. and Lespedeza spp, Especially Quercus spp. was found growing in all of the study site. 3. The average Length of the rock slopes with G. esculanta growing on was 14 m and their aspects were mostly south. 4. The G. esculanta were found growing on rocks of Crystalline Schist, Quartz, Liparite, Granite, ete. Particularly they were mostly found on granites. The gradient of the rock slopes was in the range of 22-90 degrees. 5. The mean number of individuals of G. esculanta per one rock slope ranged from 14 at Mt. Bukhan to 70 at Mt. Jrri. Their mean diameter ranged from 1.8cm at Mt. Munsu to 4.6cm at Mt, Sokri. 6. The average percentage of G. esculanta with fruit body was 17.6%. The highest value was found at Mt. Cheonhwang (24.0%). 7. When the 100 segments of rhizoid of Gyrophora esculanta cultured in Detmer's medium supplemented with kinetine 5mg/l and 2, 4-D 3mg/l, n callus of microspore origins were induced from about 20% of the segments. As the induced n callus was transplanted on the six different types of rocks, it was observed that the juvenile G. esculanta grew best on granite and the development rate of G. esculanta on the granite was about 55%.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.97-102
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• 2005

One of the key molecules involved in skin moisture is hyaluronan (hyaluronic acid, HA) with its associated water of hydration. The predominant component of the ECM (extracellular matrix) of skin is HA. It Is the primordial and the simplest of the GAGs (glycosaminoglycans), a water-sorbed macromolecule In extracellular matrix, Included between the vital cells of epidermis. In the skin, HA was previously thought to derive extlusively from dermis. But, recent studies revealed that HA could be synthesized in epidermis. Flavonoids are polyphenolic compounds that is found mainly in foods of plant origin. Kaempferol was known to increase glutathione synthesis in human keratinocyte. And quercetin blocked PPAR-meidated keratinocyte differentiation as lipoxygenase inhibitors. In this study, we sought to evaluate the effect of flavonid, kaempferol and quercetin on production HA in keratinocyte. We examined the changes of three human hyaluronan synthase genes (HASI, HAS2, HAS3) expression by semi-quantitative RT-PCR when kaempferol or quercetin was added to cultured human keratinocytes. We found that these flavonoids slightly upregulated HAS2, HAS3 mRNA after 24 h. And we investigated the effect on HA production by ELISA. When we evaluated the level of HA in culture medium after 24 h incubation. We found enhanced accumulation of HA in the culture medium. Although the effects of above flavonoids are less than retinoic acid, the data indicate that kaempferol, quercetin can dose-dependently increase the level of HA in epidermis cell line. It suggested that flavonoid, kaempferol, and quercetin increased production of HA in skin and it helped to elevate skin moisture and improve facial wrinkle.

• Journal of Korea Soil Environment Society
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• v.1 no.2
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• pp.23-34
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• 1996

The biological magnification of Pb, Cu, Zn and Fe in vascular plant, Persicaria thunbergii, Trapa japonica and Nymphoides peltata natually grown at the river and riverside and relationship between the concentration of heavy metals in these vascular plants and in the soil were investigated in the Chonju river, Chonju city. The biological magnification values of these metals in P thunbergii were founded as follows ; Pb, Cu, Zn and Fe were 80.4~254.6$mu\textrm{g}$/g, 284.6~688.4$mu\textrm{g}$/g, 635.5~1979.4$mu\textrm{g}$/g and 1160.0~3590.9$mu\textrm{g}$/g respectively. In the case of T. japonica, Pb, Cu, Zn and Fe were 107.8~306.0$mu\textrm{g}$/g, 282.7~963.0p$mu\textrm{g}$/g, 1328.3~3546.$mu\textrm{g}$/g and 656.8~9944.0$mu\textrm{g}$/g and in N. peltata, 140.1~ 193.9$mu\textrm{g}$/g, 187.7~327.3$mu\textrm{g}$/g, 1126.6~1723.6$mu\textrm{g}$/g and 611.7~1914.6$mu\textrm{g}$/g respectively. The amount of the biological magnification of heavy metals in the leaf, stem and root of these plants was generally increased in the sequence of leaf

• Journal of Bio-Environment Control
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• v.31 no.3
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• pp.230-236
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• 2022

This study was carried out to produce two-flowered seedlings, harvest them early in a greenhouse, and extend the harvest period. This study was carried out to effectively produce the second truss blooming seedlings to harvest tomatoes early and extend the harvest period. For production of the second truss blooming seedlings (one stem), the nutrient solution EC was supplied at 1.5, 2.0, 2.5 dS·m^-1, and dynamic management (3.0 → 3.5 → 4.5 dS·m^-1). The seedling period was 60 days, which was 20-40 days longer than conventional seedlings, and 10 days longer than the first truss blooming seedlings (cube seedlings). The plant height was 78 and 77 cm in EC 2.5 dS·m^-1 and dynamic management respectively, which was shorter than EC 1.5 dS·m^-1 with 88 cm. As for the EC in the cube before formulation, dynamic management had the highest EC 5.5 dS·m^-1, and the cube supplied with EC 1.5 dS·m^-1 had the lowest. The production yield by treatment did not a difference among in the second truss blooming seedlings, but the first truss blooming seedlings showed lower productivity than second truss blooming seedlings. The second truss blooming seedling were harvested 35 days after planting on June 4, the first harvest date, and the first truss blooming were harvested in 42 days on June 11th. There was no difference in plant height and root growth due to bending at frequency planting. In the study on the production of the second truss blooming seedlings (two stem), the nutrient solution EC was supplied under 2.0, 2.5, 3.0 dS·m^-1, and dynamic management (3.0 → 3.5 → 4.5 dS·m^-1). The seedling period was 90 days, which was 40-50 days longer than conventional seedlings and 10 days longer than the first truss blooming seedlings (cube seedlings). Plant height was 80 and 81 cm in EC 2.0 dS·m^-1 and 2.5 dS·m^-1 respectively, but was the shortest at 73 cm in dynamic management. EC in the medium increased as the seeding period increased in all treatments. The dynamic management was the highest with EC 5.1 dS·m^-1. There was no difference in yield among EC treatments in the second truss blooming seedlings, which had a longer seeding period of about 10 days, produced 15% more than the first truss blooming seedlings. In order to shorten the plant height of the second truss blooming seedlings, it is judged that the most efficient method is increasing the concentration of nutrient solution.

• Journal of Korean Society of Forest Science
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• v.43 no.1
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• pp.6-13
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• 1979

In order to substract the time and cost of propagation for inducing the haploid plants per each species. 500 anthers of late uninucleate microspore on early binucleate microspore stage of Robinia pseudoacacia (Fuel tree) Punius granatum (Ornamental tree). Aleurites fordii (Faty tree) and Styrax japonica (Silvicultural tree) were cultured on the modified Murashige and Skoog's medium supplemented with Kinetin, 2.4-D and NAA as growth regulators. And I observed the samples of cultured anthers under the microscope which were made by Microtoming method and Paraffin method. The results were summarized as follows: 1) Among 500 cultured anthers per each species, anther numbers inducing the diploid callus were as follow: Styrax japonica 20 (4% for the species total); Aleurites fordii 10 (2% for the species, total) and Punica granatum 45 (9% for the species total) were showed. 2) 2n Callus were induced from anther wall. but haploid callus were induced from anther locule. 3) Haploid callus were induced only in 25 anthers (5% for the species total) of Robinia pseudoacacia. 4) These haploid callus were not originated from body cell of anther wall tissue, but from reduced microspores, 5) Since already reported many herbaceous haploid plants were induced from the callus which were originated from reduced microspores, I conclude that the anther of woody plant which induced the haploid callus also will be cultured haploid plant.

• Journal of Bio-Environment Control
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• v.9 no.2
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• pp.85-93
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• 2000

This study was carried out to investigate the effects of several substrates and ratio of NO3--N to NH4--N in nutrient solution on growth, yield and mineral uptake of sweet pepper(Capsicum annuum L.) in bag culture. The content of minerals such as P, K, Ca and Mg showed difference in concentration among media: P and Mg were the highest in vermiculite in vermiculti+rice hull, K in perlite+vermiculite and Ca in perlite+peatmoss; with the lowest in the single perlite medium respectively, Ca of mineral made fairly higest level in concentration compared with the others in all of the media. The concentration of mineral content was lower in the ratio of 8:2 than that of 10:0. Admitting that the pH made difference depending on the kind of substrates and ratios of NO3--N to NH4--N. The pH of 10:0 ratio in all the substrates was higher and more stable than that of 8:2. The range of EC in all the substrates showed from 1.78ds·m-1 to 2.10 ds·m-1, which was optimum range for growth of sweet pepper, and range of EC is larger in 8:2 ratio than that in 10:0 ratio. Plant height and stem diameter were nothing to do with the kind of substrates, but leaf area was the largest at vermiculite+rice hull of the 8:2 ratio, fresh and dry weights were heavier at peatmoss+carbonized rice hull, but were the lightest at perlite. All indexes related to the growth which had something to do with the kind of substrates higher in 8:2 ratio than those in 10:0 ratio. The number of fruit and fresh weight related to the ratio of the 8:2 were the highest as 17.5 at vermiculite+rice hull with 1,588g of fresh weight, while the yield from perlite was the lowest. The number of fruit was the highest as 16.4 at virmiculite+rice hull, yield was the higest as 1,394a at perlite+ peatmoss. The yield of 8:2 ratio at all substrates was higher than that of 10:0 ratio. Of the mineral content related to the plant part, K+ and Mg2+ were higher in concentration at leaf; Ca2+ were higher at root; PO4- was higher at stem and fruit; The content of mineral showed no difference between the ratio of the 8:2 and the ratio of the 10:0 with no regrading to the difference of mineral content among substrates; and K+, Ca2+, and Mg2+ uptake of sweet pepper were higher at 10:0 ratio than that of 8:2; 2while PO4- uptake of sweet pepper was lower at 10:0 ratio than that of 8:2 ratio.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.37 no.4
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• pp.81-97
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• 2019

The purpose of this study is to examine what people in Korea recognize the cultural symbolism and the planting patterns of 'Guǐmok', pagoda tree(Sophora japonica). The species planted in the 'Guǐjeong' was empirically investigated and analyzed to determine which species of pagoda tree or Zelkova tree(Zelkova serrata) was taken through literature surveys, field surveys, and interviews with persons. This 'Guǐjeong' was combined to track how the culture of the 'Guǐmok' planting introduced in China was ultimately accepted and transformed in Korea. In this study, we tried to analyze the meaning implicit in the mystery while checking the distribution of the mystery and the form of the mystery, the name of the pavilion and its relevance to the contrast medium. Essentially, the trees that govern the characterization of the nectar plant, regardless of the region, are considered to be a pagoda tree, which is considered an internal factor in which the pagoda tree culture was not completely transformed into a zelkova tree. It was recognized throughout the Joseon Dynasty that the species representing 'Prime ministers(三公)' was judged from all the Joseon Dynasty periods, based on the builder of Guǐjeong's Aho(雅號) and Dangho(堂號). It was confirmed that the tree was very likely to be planted in place of the painting tree. But now is selectively zelkova tree is in accordance with the preparation of planting site conditions and areas on behalf of the Change is very high probability that is planted. Cultural variables that led to the cultural transformation of the 'Guǐmok' seem to have been deeply involved in the geographical space of China and Korea, Confucian practices of the Choson society, comings and goings and letter bridge, and network strength with China through the book spread. In addition, the culture of 'Guǐmok' is presumed to have led to cultural custom of the upper class, not the whole class, in the Yeongnam region, it can be said that the independent adaptation to act to recognize 'Guǐmok' as a pagoda tree, that is a Sophora japonica, has occurred very strongly. The difference between the cultural areas of Yeongnam and non-Yeongnam is also considered to be an internal factor that has played a major role in the cultural transformation of planting of 'Guǐmok'.