• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.247-254
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• 2019

Flavonoids are widely distributed secondary metabolites in plants that have a variety biological functions, as well as beneficial biological and pharmacological activities. In barley (Hordeum vulgare L.), for example, high levels of saponarin accumulate during primary leaf development. However, the effect of saponarin biosynthetic pathway genes on the accumulation of saponarin in barley is poorly understood. Accordingly, the aim of the present study was to examine the saponarin contents and expression levels of saponarin biosynthetic pathway genes [chalcone synthase (CHS), chalcone isomerase (CHI), and UDP-Glc:isovitexin 7-O-glucosyltransferase (OGT)] during early seedling developmental and under several abiotic stress conditions. Interestingly, the upregulation of HvCHS, HvCHI, and HvOGT during early development was associated with saponarin accumulation during later stages. In addition, exposure to abiotic stress conditions (e.g., light/dark transition, drought, and low or high temperature) significantly affected the expression of HvCHS and HvCHI but failed to affect either HvOGT expression or saponarin accumulation. These findings suggested that the expression of HvOGT, which encodes an enzyme that catalyzes the final step of saponarin biosynthesis, is required for saponarin accumulation. Taken together, the results of the present study provide a basis for metabolic engineering in barley plants, especially in regards to enhancing the contents of useful secondary metabolites, such as saponarin.

• Journal of Soil and Groundwater Environment
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• v.23 no.1
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• pp.74-84
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• 2018

Pot experiments were conducted to assess the applicability of ferric chloride ($FeCl_3$) as a washing agent for laboratory scale in-situ soil washing of paddy soil contaminated with Pb. During the monitoring period for nearly 90 days, the concentrations of Fe and Mn in the soil solution were lower than that of control soil due to lime ($Ca(OH)_2$) amendment for pH recovery. Lime amendment also affected solubility and fractionation of Pb into soil matrix. The result showed that Pb concentrations of soil solution were consistently lower than that of control soil, and the concentration in the exchangeable fraction in washed soil decreased from 13 to 2 mg/kg. There was no significant difference of biomass yield of rice plant in each pots, and Pb contents in rice roots and grains in washed soil decreased to 50 and 78%, respectively, of the control soil. Therefore, $FeCl_3$ could be used as an acceptable in-situ washing agent for agricultural paddy soil if appropriate soil pH management is subsequently practiced.

• Journal of the Korean Society for Heat Treatment
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• v.12 no.2
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• pp.136-144
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• 1999

The study on the wear characteristics of compound layers formed during gaseous nitrocarburising in the medium carbon boron steels and the plain carbon steel has been carried out by using a pin-on-disc type wear test machine under the oil lubricating condition at room temperature and by varying applied loads, sliding speeds and wear distances. Values of friction coefficient measured at the sliding speed of 0.4m/sec under the oil lubricating condition have been shown to decrease considerably with increasing applied load and become gradually a constant value as load is increased to a higher value, showing that the transition load for friction coefficient appears at an applied load of 247.2N. The length and volume wear rates of compound layer have been revealed to relatively constantly increase, also showing that the wear life per unit thickness of compound layer turns out to be superior as porous layer has a denser and thinner appearance. As the sliding speed increases during wear test performed by varying sliding speed at a load of 63.2N under the oil lubricating condition for medium carbon boron steel nitrocarburised in gas atmosphere, the wear rate has been found to increase, the friction coefficient to decrease and the wear life per unit thickness of compound layer to decrease considerably.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.1
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• pp.1-10
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• 2004

The climate of Minamiakikawa forest in Japan is similar to that of Mt. Jiri in South Korea. There is a large development plan for Minamiakikawa forest, and a change in the species composition is expected. This study was initiated to compare forest transition caused by artificial perturbations in Korea and Japan. Long-term field observations on species composition are reported. We found 98 families, 231 genera, 315 species, 29 varieties, and 8 races, making a total of 352 classification groups of higher plants in the Minamiakikawa forest area. Among them, 11 families, 12 species and 2 varieties are rare or endangered. The study area is dominated by Cryptomerica japonica and Chamaecyparis obtusa. The time and restoration effects on secondary and latent forestation consider the development of the Quercus mongolica community, the Quercus serrata community, and deciduous-broadleaved tree ascension. This indicates that the forest has been restored to Abies firma, Pinus densiflora or Cryptomeria japonica and Fagus japonica, which is considered latent natural forestation of the area in a natural transfer.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.329-334
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• 2003

D-type cyclins are believed to regulate the G1 to S phase transition in response to nutrient and hormonal signals. We investigated the expression characteristics of the key cell-cycle regulators, mitotic and G1 cyclins in potato (Solanum tuberosum L.). We isolated D-type cyclin gene from potato and it was classified as D3 cyclin by sequence similarities and a phylogenetic analysis, and named as StcycD3;1. The accumulation of transcripts was predominantly associated with mitotically active organs, such as stolons, roots, flowers, leaves, and stems. Transcription of StcycD3;1 can be induced by sucrose.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.72-72
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• 2018

The root of Angelica gigas Nakai (AGN) is used as a traditional herbal medicine in Korea for the treatment of many diseases. However, a major challenge associated with the usage of the active compounds from AGN is their poor water solubility. Therefore, this work aimed to enhance the solubility of active compounds by a chemical (viz. surfactant) and physical (hot melt extrusion) crosslinking method (CPC). Infrared Fourier transform spectroscopy (FT-IR) revealed multiple peaks in extrudate solids representing new functional groups including carboxylic acid, alkynes and benzene derivatives. Differential scanning calorimetry (DSC) analysis of the extrudate showed lower glass transition temperature (Tg) and lower enthalpy (${\Delta}H$) (Tg: $43^{\circ}C$; ${\Delta}H$: <6 (J/g)) compared to the non-extrudate (Tg $68.5^{\circ}C$; ${\Delta}H$: 123.2) formulations. X-ray powder diffraction (XRD) analysis revealed amorphization of crystal materials in extrudate solid. In addition, nanonization, enhanced solubility and higher extraction of phenolic compounds were achieved in the extrudate solid. Among the different extrudates, acetic acid- and Span 80-mediated formulations showed superior extractions. We conclude that the CPC method successfully enhanced the production of amorphous nano dispersions from extrudate solid formulations.

• Journal of the Korea Institute of Building Construction
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• v.16 no.4
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• pp.305-311
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• 2016

This study examined the long-term inelastic characteristics, including unrestrained shrinkage and creep, of low-heat cement concrete under different ambient curing temperatures. To achieve the designed compressive strength of 42MPa, water-to-binder ratios were selected to be 27.5, 30, and 32.5% for curing temperatures of 5, 20, and $40^{\circ}C$, respectively. Test results showed that the shrinkage strains of concrete mixtures tended to decrease with the decrease in curing temperature because of the delayed evaporation of internal capillary and gel waters. Meanwhile, creep strains were higher in concrete specimens under lower curing temperature due to the occurrence of the transition temperature creep. The design models of KCI provision gave better accuracy in comparison with test results than those of ACI 209, although a correction factor for low-heat cement needs to be established in the KCI provision.

• Bulletin of the Korean Chemical Society
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• v.34 no.5
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• pp.1525-1529
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• 2013

Pseudo-first-order rate constants ($k_{obsd}$) have been measured spectrophotometrically for the nucleophilic substitution reactions of 2-pyridyl thionobenzoate (5b) with alkali-metal ethoxides (EtOM, $M^+=Li^+$, $Na^+$, $K^+$, and 18-crown-6-ether complexed $K^+$) in anhydrous ethanol at $25.0{\pm}0.1^{\circ}C$. The plots of $k_{obsd}$ vs. $[EtOM]_o$ curve upward regardless of the nature of the $M^+$ ions, while those of $k_{obsd}/[EtO^-]_{eq}$ vs. $[EtO^-]_{eq}$ are linear with a positive intercept. Dissection of $k_{obsd}$ into $k_{EtO^-}$ and $k_{EtOM}$ (i.e., the second-order rate constants for the reactions with the dissociated $EtO^-$ and ion-paired EtOM, respectively) has revealed that the ion-paired EtOM is more reactive than the dissociated $EtO^-$, and $M^+$ ions catalyze the reactions in the order $K^+$ < $Na^+$ < $Li^+$ < 18C6-complexed $K^+$. The plot of log $k_{EtOM}$ vs. $1/r_{Stokes}$ results in an excellent linear correlation, indicating that the reactions are catalyzed by the solvated $M^+$ ions but not by the bare $M^+$ ions. The reactions of 5b with EtOM have been concluded to proceed through a six-membered cyclic TS, in which the solvated $M^+$ ions increase the electrophilicity of the reaction center and the nucleofugality of the leaving group.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.72-79
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• 2013

Inactivation of the gene coding for dihydroflavonol 4-reductase (DFR) is responsible for the color difference between red and yellow onions (Allium cepa L.). Two inactive DFR-A alleles, DFR-$A^{PS}$ and DFR-$A^{DEL}$, were identified in our previous study. A functional marker was developed on the basis of the premature stop codon that inactivated the DFR-$A^{PS}$ allele. A derived cleaved amplified polymorphic sequences (dCAPS) primer was designed to detect the single nucleotide polymorphism, an A/T transition, which produced the premature stop codon. Digested PCR products clearly distinguished the homozygous and heterozygous red $F_2$ individuals. Meanwhile, to develop a molecular marker for detection of the DFR-$A^{DEL}$ allele in which entire DFR-A gene was deleted, genome walking was performed and approximately 3 kb 5' and 3' flanking sequences of the DFR-$A^R$ coding region were obtained. PCR amplification using multiple primers binding to the extended flanking regions showed that more of the extended region of the DFR-A gene was deleted in the DFR-$A^{DEL}$ allele. A dominant simple PCR marker was developed to identify the DFR-$A^{DEL}$ allele using the dissimilar 3' flanking sequences of the DFR-A gene and homologous DFR-B pseudogene. Distribution of the DFR-$A^{PS}$ and DFR-$A^{DEL}$ alleles in yellow onion cultivars bred in Korea and Japan was surveyed using molecular makers developed in this study. Results showed predominant existence of the DFR-$A^{PS}$ allele in yellow onion cultivars.

• Proceedings of the Korean Information Science Society Conference
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• 2004.04b
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• pp.541-543
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• 2004

능동적 학습(active learning)은 제한된 시간과 인력으로 가능한 정확도가 높은 분류기(classifier)를 생성하기 위하여, 훈련집합에 추가할 예제 즉 문의예제(query example)의 선정과 확장된 훈련집합으로 다시 학습하는 과정을 반복하여 수행한다. 능동적 학습의 핵심은 사용자에게 카테고리(category) 부여를 요청할 문의예제를 선정하는 과정에 있다. 효과적인 문의예제를 선정하기 위하여 다양한 방안들이 제안되었으나, 이들은 매 문의단계마다 하나의 문의예제를 선정하는 경우에 가장 적합하도록 고안되었다. 능동적 학습이 복수의 예제를 사용자에게 문의할 수 있다면, 사용자는 문의예제들을 서로 비교해 가면서 작업할 수 있으므로 카테고리 부여작업을 보다 빠르고 정확하게 수행할 수 있을 것이다. 또한 충분한 인력을 보유한 상황에서는, 카테고리 부여작업을 병렬로 처리할 수 있어 전반적인 학습시간의 단축에 큰 도움이 될 것이다. 하지만, 각 예제의 문의예제로써의 적합 정도를 추정하면 유사한 예제들은 서로 비슷한 수준으로 평가되므로, 기존의 방안들을 복수의 문의예제 선정작업에 그대로 적용할 경우, 유사한 예제들이 문의예제로 동시에 선정되어 능동적 학습의 효율이 저하되는 현상이 나타날 수 있다. 본 논문에서는 특정 예제를 문의예제로 선정하면 이와 일정 수준이상 유사한 예제들은 해당 예제와 함께 문의예제로 선정하지 않음으로써, 이러한 문제점을 극복할 수 있는 방안을 제안한다. 제안한 방안을 문서분류 문제에 적용해 본 결과 기존 문의예제 선정방안으로 복수 문의예제를 선정할 때 발생할 수 있는 문제점을 상당히 완화시킬 있을 뿐 아니라, 복수의 문의예제를 선정하더라도 각 문의 단계마다 하나의 예제를 선정하는 경우에 비해 큰 성능의 저하가 없음을 실험적으로 확인하였다./$m\ell$로 나타났다.TEX>${HCO_3}^-$ 이온의 탈착은 서서히 진행되었다. R＆D investment increases are directly not liked to R＆D productivities because of delays and side effects during transition periods between different stages of technology development. Thus, It is necessary to develope strategies in order to enhance efficiency of technological development process by perceiving the switching pattern. 기여할 수 있을 것으로 기대된다. 것이다.'ity, and warm water discharges from a power plant, etc.h to the way to dispose heavy water adsorbent. Through this we could reduce solid waste products and the expense of permanent disposal of radioactive waste products and also we could contribute nuclear power plant run safely. According to the result we could keep the best condition of radiation safety super vision and we could help people believe in safety with Radioactivity wastes control for harmony with Environ