• Parasites, Hosts and Diseases
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• v.50 no.1
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• pp.53-56
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• 2012

Treatment of hydatid disease is mainly surgical, with medical treatment being reserved as a coadjuvant treatment. Use of effective scolicidal agents during surgery of cystic echinococcosis is essential to reduce the recurrence rate. The goal of this study was to evaluate the in vitro scolicidal effects of hydroalcoholic extracts of $Satureja$ $khuzestanica$ leaves and aqueous extracts of $Olea$ $europaea$ leaves on hydatid cyst protoscolices. $Echinococcus$ $granulosus$ protoscolices were collected from the liver of sheep infected with the hydatid cyst. Various concentrations of plant extracts were used in different exposure times for viability assay of protoscolices. Among the olive leaf extracts tested, 0.1% and 0.01% concentrations had strong scolicidal effects in 120 min. $S.$ $khuzestanica$ 0.1% had very strong scolicidal effects in 30, 60, and 120 min of exposure times and the mortality rate decreased with the lower concentration. The finding have shown that the scolicidal activity of $S.$ $khuzestanica$ against cystic echinococosis protoscolices were more effective, while the $O.$ $europaea$ extract showed less effects.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.1
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• pp.91-99
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• 2007

The research was carried out to define the morphological and growth characteristics of Poa pratensis L. (Kentucky bluegrass) 'Pureundul' native to Korea. Poa pratensis 'Pureundul' was collected from the belt highway around Mt. Uam located in Naedeok-dong, Cheongju City, Chungcheongbuk-do Province on May 5, 1995. The morphological characteristics of 'Pureundul' were compared with those of 205 individuals of native Poa pratensis collected throughout the nation from June 11, 2000 to June 21, 2003. The findings were as follows : The height of plant was 28.2cm but increased to 40.0cm at the flowering time. The height of first node from the base was 6.8cm and the length and width of leaf were 17.3cm and 2.9mm, respectively. The length of a spike was 7.9cm. Based on these morphological characteristics, 'Pureundul' was defined medium type Kentucky bluegrass. Visual quality(1~9), visual color and visual density were measured. The visual quality, color and density of 'Pureundul' were not superior to those of introduced species but above the average other native species. The damages from the diseases during summer resulted in poorer visual quality of color of 'Pureundul'. But the spread of the diseases was slower in 'Pureundul' than other native species, and accordingly 'Pureundul' didn't suffer from more quality deterioration from July to September during which the diseases broke out. Entering fall, 'Pureundul' showed high quality comparable to that of the introduced species. If 'Pureundul' is put to breeding by implementing pest control in summer and introducing disease tolerant genes, it will be used to make greens of greater quality than now.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.2
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• pp.91-106
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• 2013

Exserohilum turcicum is considered serious destructive disease of maize (Zea mays L.) in North Korea. This study aimed to understand genetic inheritance and combining ability of newly bred lines of maize tolerant to E. turcicum by diallel crosses. Three diallel sets for two different ecological regions and one agronomic trait; eastern (E), northern (N) and stay green (SG) involving 29 inbred lines were tested in eight locations of 2000 and 2001. E. turcicum infections were under natural conditions, respectively. Lines used were selected for high yield potential in test crosses with good agronomic traits and tolerance to biotic and abiotic stresses. Selection for race specific high resistance to biotic stresses was avoided to select quantitatively inherited genes. Host plant responses to E. turcicum were rated on a scale of 1 (highly tolerant) to 9 (highly susceptible). Highly significant variations were recorded in all trials. General combining ability (GCA) mean square was roughly twice that of specific combining ability (SCA). The genotype (G) by environment (E) interaction was highly significant. The overall results of genetic studies in three diallel sets show that genetic control for inbred tolerance to E. turcicum is polygenic and quantitatively inherited. New inbreds; E-3, N-1 and SG-4 confer better tolerance to E. turcicum than the widely used inbreds; Mo17, and B73. Proper use of genetic information from this study shall increase of corn production under high E. turcicum infection in the Far Eastern Regions of Korea and China.

• Journal of Microbiology and Biotechnology
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• v.29 no.12
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• pp.1975-1981
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• 2019

Recently, outbreaks of food-borne diseases linked to fresh produce have been an emerging public health concern worldwide. Previous research has shown that when human pathogens co-exist with plant pathogens, they have improved growth and survival rates. In this study, we have assessed whether Escherichia coli O157:H7 benefits from the existence of a phytopathogenic bacterium and the underlying mechanisms were further investigated. When Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and E. coli O157:H7 were co-inoculated by either dipping or infiltration methods, the populations of E. coli O157:H7 increased; however, no effect was observed when type three secretion system (T3SS) mutants were used instead, suggesting that E. coli O157:H7 benefits from the presence of Pst DC3000. In addition, this study confirmed that the E. coli O157:H7 populations increased when they occupied the tomato leaf intercellular space; this colonization of the interior of the leaves was possible due to the suppression of the PAMP-triggered immunity (PTI) by Pst DC3000, in particular with the AvrPto effector. In conclusion, our data support a plausible model that E. coli O157:H7 benefits from the presence of Pst DC3000 via AvrPto suppression of the PTI resistance.

• Korean journal of applied entomology
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• v.23 no.4 s.61
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• pp.209-214
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• 1984

Since 1980, burley tobacco plants grown mainly in the western hat of the Korea. have shown two new types of disease symptom. Both symptoms were found to be caused by two different PVY strains : the vein banding type by a PVY strain designated as PVY-VB and necrosis on leaf veins by a PVY strain designated as PVY-VN, Identification of the PVY strains was based on host range test. aphid( Myzus Persicae) transmission test, physical properties, serology, and observation of virus particle morphology. The virus particles were measured to be about 730 nm without any difference in shape or dimensions between the two strains. Both strains also gave a positive reaction to the PVY antiserum in SDS-agar gel double diffusion test. These strains, however, gave a negative reaction to the tobacco etch virus and tobacco vein mottling virus antisera.

• Korean Journal of Agricultural and Forest Meteorology
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• v.3 no.3
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• pp.156-162
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• 2001

In order to monitor local climatic information, twelve automated weather stations (AWS) were installed in alpine area by the Alpine Agricultural Experiment Station, Rural Development Administration (RDA), at the field of major crop located in around highland area, and collected data from 1993 to 2000. Hourly measurements of air and soil temperature (underground 10 cm,20 cm), relative humidity, wind speed and direction, precipitation, solar radiation and leaf wetness were automatically performed and the data could be collected through a public phone line. Datalogger was selected as CR10X (Campbell scientific, LTD, USA) out of consideration for sensers' compatibility, economics, endurance and conveniences. All AWS in alpine area were combined for net work and daily climatic data were analyzed in text and graphic file by program (Chumsungdae, LTD) on 1 km $\times$ 1 km grid tell basis. In this analysis system, important multi-functionalities, monitoring and analysis of local climatic information in alpine area was emphasized. The first objective was to obtain the output of a real time data from AWS. Secondly, daily climatic normals for each grid tell were calculated from geo-statistical relationships based on the climatic records of existing weather stations as well as their topographical informations. On 1 km $\times$ 1 km grid cell basis, real time climatic data from the automated weather stations and daily climatic normals were analyzed and graphed. In the future, if several simulation models were developed and connected with this system it would be possible to precisely forecast crop growth and yield or plant disease and pest by using climatic information in alpine area.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.558-562
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• 1996

The growth and yield of Job's tears (Coix lacryma-jobi L.) under the flooded paddy and upland (dry paddy) field conditions were compared at three sowing dates and two planting densities. Job's tears grown under flooded paddy field was much shorter in plant height, but greater in number of tillers than those grown under upland. Photosynthesis rate of Job's tears grown under flooded paddy field was higher and the weight of dry roots heavier but the damages of pest and leaf blight disease smaller than those grown under upland. For the above mentioned reasons, the grain yield of Job's tears grown under flooded paddy field was higher by up to 85% than that grown under upland. There was no significant difference in grain yield between the planting densities. The earlier sowing brought about the less grain yield in upland field condition, while sowing plot on the 15th of May showed the highest grain yield in the flooded paddy field condition.

• Molecules and Cells
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• v.40 no.8
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• pp.587-597
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• 2017

MicroRNAs (miRNAs) are essential small RNA molecules that regulate the expression of target mRNAs in plants and animals. Here, we aimed to identify miRNAs and their putative targets in Hibiscus syriacus, the national flower of South Korea. We employed high-throughput sequencing of small RNAs obtained from four different tissues (i.e., leaf, root, flower, and ovary) and identified 33 conserved and 30 novel miRNA families, many of which showed differential tissuespecific expressions. In addition, we computationally predicted novel targets of miRNAs and validated some of them using 5' rapid amplification of cDNA ends analysis. One of the validated novel targets of miR477 was a terpene synthase, the primary gene involved in the formation of disease-resistant terpene metabolites such as sterols and phytoalexins. In addition, a predicted target of conserved miRNAs, miR396, is SHORT VEGETATIVE PHASE, which is involved in flower initiation and is duplicated in H. syriacus. Collectively, this study provides the first reliable draft of the H. syriacus miRNA transcriptome that should constitute a basis for understanding the biological roles of miRNAs in H. syriacus.

• Journal of the Korean Society of Industry Convergence
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• v.22 no.1
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• pp.61-69
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• 2019

All the parts of lotus, including the seed, rhizome, leaf, stalk, petal, anther, pericarp, and fruit receptacle, have been used in traditional medicine system as a health beneficial supplement. However the most usually used from lotus plant is only the root. Therefore in this study, it will be discussed more the utilization of other parts of the lotus, namely the flower of lotus. The petals and stamens of lotus actually are also rich in bioactive components such as flavonoids and alkaloids, are used in the treatment of tissue inflammation, cancer, skin disease, and also for us as antidotes. One of the biotechnological process that can be used to improve the nutritional content, sensory, and also antioxidant activities is fermentation process. The final product desired from the fermentation process in this study is vinegar. The microbial strain powder used is Uinkin fermented powder with three variations of fermentation. The variations given in this study were initial sugar 32%, 24%, and 14% with the same fermentation temperature, $35^{\circ}C$ for 3 months. The results obtained showed that the pH value and sugar content of products during the fermentation process were decreasing during the fermentation process, with total polyphenol content of $283.7{\pm}97.6mg/100g\;QAE$, and total flavonoid content of $3.3{\pm}0.0mg/100g\;QAE$. For the DPPH radical scavenging ability of the fermentation product also increased in a concentration dependent manner, with ORAC activity of the product showed a high activity of $20.7{\pm}0.41{\mu}M$ TE. Therefore, fermentation process can be the one of method for improving the product. The efficiency of lotus flower vinegar fermentation can be reached with an initial sugar condition of 25% (sample B).

• Microbiology and Biotechnology Letters
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• v.32 no.3
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• pp.256-264
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• 2004

A new primer set was developed for the detection and identification of Xanthomonas oryzae pv. oryzae, the bacterial leaf blight (BLB) pathogen in rice plant. The nucleotide sequence of hpaA gene was determined from X. o. pv. oryzae str. KACC10331, and the sequence information was used to design primers for the application of the polymerase chain reaction (PCR). The nucleotide sequence of hpaA from X. o. pv. oryzae str. KACC 10331 was aligned with those of X. campestris pv. vesicatoria, X. campestris pv. campestris, X. axonopodis pv. citri, and X. axonopodis pv. glycines. Based on these results, a primer set(XOF and XOR) was designed for the specific detection of hpaA in X. o. pv. oryzae. The length of PCR products amplified using the primer set was 534-bp. The PCR product was detected from only X. o. pv. oryzae among other Xanthomonas strains and reference bacteria. This product was used to confirm the conservation of hpaA among Xanthomonas strains by Southern-blotting. Furthermore, PCR amplification with XOF and XOR was used to detect the pathogen in an artificially infected leaf. The sensitivity of PCR detection in the pure culture suspension was also determined. This PCR-based detection methods will be a useful method for the detection and identification of X. o. pv. oryzae as well as disease forecasting.