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http://dx.doi.org/10.4014/jmb.1907.07072

Pseudomonas syringae pv. tomato DC3000 Improves Escherichia coli O157:H7 Survival in Tomato Plants  

Namgung, Min (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University)
Lim, Yeon-Jeong (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University)
Kang, Min Kyu (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University)
Oh, Chang-Sik (Department of Horticultural Biotechnology, Kyung Hee University)
Park, Duck Hwan (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University)
Publication Information
Journal of Microbiology and Biotechnology / v.29, no.12, 2019 , pp. 1975-1981 More about this Journal
Abstract
Recently, outbreaks of food-borne diseases linked to fresh produce have been an emerging public health concern worldwide. Previous research has shown that when human pathogens co-exist with plant pathogens, they have improved growth and survival rates. In this study, we have assessed whether Escherichia coli O157:H7 benefits from the existence of a phytopathogenic bacterium and the underlying mechanisms were further investigated. When Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and E. coli O157:H7 were co-inoculated by either dipping or infiltration methods, the populations of E. coli O157:H7 increased; however, no effect was observed when type three secretion system (T3SS) mutants were used instead, suggesting that E. coli O157:H7 benefits from the presence of Pst DC3000. In addition, this study confirmed that the E. coli O157:H7 populations increased when they occupied the tomato leaf intercellular space; this colonization of the interior of the leaves was possible due to the suppression of the PAMP-triggered immunity (PTI) by Pst DC3000, in particular with the AvrPto effector. In conclusion, our data support a plausible model that E. coli O157:H7 benefits from the presence of Pst DC3000 via AvrPto suppression of the PTI resistance.
Keywords
AvrPto; E. coli O157:H7; effector; food-borne disease; Pseudomonas syringae pv. tomato DC3000; tomato;
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1 Hauck P, Thilmony R, He SY. 2003. A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants. Proc. Natl. Acad. Sci. USA 100: 8577-8582.   DOI
2 Nürnberger T, Brunner F, Kemmerling B, Piater L. 2004. Innate immunity in plants and animals: striking similarities and obvious differences. Immunol. Rev. 198: 249-266.   DOI
3 Xin X, He SY. 2013. Pseudomonas syringae pv. tomato DC3000: a model pathogen for probing disease susceptibility and hormone signaling in plants. Annu. Rev. Phytopathol. 51: 473-498.   DOI
4 Almeida DP, Huber DJ. 1999. Apoplastic pH and inorganic ion levels in tomato fruit: a potential means for regulation of cell wall metabolism during ripening. Physiol. Plantarum 105: 506-512.   DOI
5 Doyle MP, Erickson MC. 2008. Summer meeting 2007 - the problems with fresh produce: an overview. J. Appl. Microbiol. 105: 317-330.   DOI
6 Melotto M, Panchal S, Roy D. 2014. Plant innate immunity against human bacterial pathogens. Front Microbiol. 5: doi: 10.3389/fmicb.2014.00411.
7 Gu G, Hu J, Cevallos-Cevallos JM, Richardson SM, Bartz JA, van Bruggen AHC. 2011. Internal colonization of Salmonella enterica serovar Typhimurium in tomato plants. PLoS One 6: e27340.   DOI
8 Roy D, Ranchal W, Rosa BA, Melotto M. 2013. Escherichia coli O157:H7 induces plant immunity than Salmonella enterica Typhimurium SL1344. Phyhtopathology 103: 326-332.   DOI
9 Seo S, Matthews KR. 2012. Influence of the plant defense response to Escherichia coli O157:H7 cell surface structures on survival of that enteric pathogen on plant surfaces. Appl. Environ. Microbiol. 78: 5882-5889.   DOI
10 Meng F, Altier C, Martin GB. 2014. Salmonella colonization activates the plant immune system and benefits from association with plant pathogenic bacteria. Environ. Microbiol. 15: 2418-2430.   DOI
11 Simko I, Zhou Y, Brandl M. 2015. Downy mildew disease promotes the colonization of romain lettuce by Escherichia coli O157:H7 and Salmonella enterica. BMC Microbiol. 15: 19. doi: 10.1186/s12866-015-0360-5.   DOI
12 Nguyen HP, Chakravarthy S, Velasquez AC, McLane HL, Zeng L, Nakayashiki H, et al. 2010. Methods to study PAMP-triggered immunity using tomato and Nicotiana benthamiana. Mol. Plant Microbe Interact. 23: 991-999.   DOI
13 Berger CN, Sodha SV, Shaw RK, Griffin PM, Pink D, Hand P, et al. 2010. Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environ. Microbiol. 12: 2385-2397.   DOI
14 Wright KM, Chapman S, McGeachy K, Humphris S, Campbell E, Toth IK, et al. 2013. The endophytic lifestyle of Escherichia coli O157:H7: quantification and internal localization in roots. Phytopathology 103: 333-340.   DOI
15 Barak JD, Schroeder BK. 2012. Interrelationships of food safety and plant pathology: the life cycle of human pathogens on plants. Annu. Rev. Phytopathol. 50: 241-266.   DOI
16 Watanabe Y, Ozasa K, Mermin JH, Griffin PM, Masuda K, Imashuku S, et al. 1999. Factory outbreak of Escherichia coli O157:H7 infection in Japan. Emerg. Infect. Dis. 5: 424-428.   DOI
17 Solomon EB, Pang HJ, Matthews KR. 2003. Persistence of Escherichia coli O157:H7 on lettuce plants following spray irrigation with contaminated water. J. Food Prot. 66: 2198- 2202.   DOI
18 Kroupitski Y, Golberg D, Belausov E, Pinto R, Swartzberg D, Granot D, et al. 2009. Internalization of Salmonella enterica in leaves is induced by light and involves chemotaxis and penetration through open stomata. Appl. Environ. Microbiol. 75: 6076-6086.   DOI
19 Thilmony R, Underwood W, He SY. 2006. Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7. Plant J. 46: 34-53.   DOI
20 Potnis N, SotoArias JP, Cowles KN, van Bruggen AH, Jones JB, Barak JD. 2014. Xanthomonas perforans colonization influences Salmonella enterica in the tomato phyllosphere. Appl. Environ. Microbiol. 80: 3173-3180.   DOI
21 Abramovitch RB, Kim Y-J, Chen S, Dickman MB, Martin GB. 2003. Pseudomonas type III effector AvrPtoB induces plant disease susceptibility by inhibition of host programmed cell death. EMBO J. 22: 60-69.   DOI
22 Chang JH, Rathjen JP, Bernal AJ, Staskawicz BJ, Michelmore RW. 2000. AvrPto enhances growth and necrosis caused by Pseudomonas syringae pv. tomato in tomato lines lacking either Pto or Prf. Mol. Plant Microbe Interact. 13: 568-571.   DOI
23 Shan L, Thara VK, Martin GB, Zhou JM, Tang X. 2000. The Pseudomonas AvrPto protein is differentially recognized by tomato and tobacco and is localized to the plant plasma membrane. Plant Cell 12: 2323-2338.   DOI
24 Wei H-L, Chakravarthy S, Mathieu J, Helmann TC, Stodghill P, Swingle B, et al. 2015. Pseudomonas syringae pv. tomato DC3000 type III secretion effector polymutants reveal an interplay between HopAD1 and AvrPtoB. Cell Host Microbe 17: 752-762.   DOI
25 Shan L, He P, Li J, Heese A, Peck SC, Nurnberger T, et al. 2008. Bacterial effectors target the common signaling partner BAK1 to disrupt multiple MAMP receptor-signaling complexes and impede plant immunity. Cell Host Microbe 4: 17-27.   DOI