Browse > Article

PCR-Based Sensitive Detection and Identification of Xanthomonas oryzae pv. oryzae  

Lee, Byoung-Moo (National Institute of Agricultural Biotechnology(NIAB), Rural Development Administration(RDA))
Park, Young-Jin (National Institute of Agricultural Biotechnology(NIAB), Rural Development Administration(RDA))
Park, Dong-Suk (National Institute of Agricultural Biotechnology(NIAB), Rural Development Administration(RDA))
Kim, Jeong-Gu (National Institute of Agricultural Biotechnology(NIAB), Rural Development Administration(RDA))
Kang, Hee-Wan (Graduate school of Bio-&)
Noh, Tae-Hwan (Information Technology, Hankyong National University)
Lee, Gil-Bok (Honam Agricultural Research Institute, NICS)
Ahn, Joung-Kuk (National Institute of Agricultural Biotechnology(NIAB), Rural Development Administration(RDA))
Publication Information
Microbiology and Biotechnology Letters / v.32, no.3, 2004 , pp. 256-264 More about this Journal
Abstract
A new primer set was developed for the detection and identification of Xanthomonas oryzae pv. oryzae, the bacterial leaf blight (BLB) pathogen in rice plant. The nucleotide sequence of hpaA gene was determined from X. o. pv. oryzae str. KACC10331, and the sequence information was used to design primers for the application of the polymerase chain reaction (PCR). The nucleotide sequence of hpaA from X. o. pv. oryzae str. KACC 10331 was aligned with those of X. campestris pv. vesicatoria, X. campestris pv. campestris, X. axonopodis pv. citri, and X. axonopodis pv. glycines. Based on these results, a primer set(XOF and XOR) was designed for the specific detection of hpaA in X. o. pv. oryzae. The length of PCR products amplified using the primer set was 534-bp. The PCR product was detected from only X. o. pv. oryzae among other Xanthomonas strains and reference bacteria. This product was used to confirm the conservation of hpaA among Xanthomonas strains by Southern-blotting. Furthermore, PCR amplification with XOF and XOR was used to detect the pathogen in an artificially infected leaf. The sensitivity of PCR detection in the pure culture suspension was also determined. This PCR-based detection methods will be a useful method for the detection and identification of X. o. pv. oryzae as well as disease forecasting.
Keywords
hpaA; polymerase chain reaction; primer; Xanthomonas oryzae pv. oryzae;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Polymerase chain reation used in the development of a DNA probe to identify Erwinia stewartii, a bacterial pathogen of maize /
[ Blakemore, E. J. A.;J. C. Reeves;S. F. L. Ball ] / Seed Sci. Technol.
2 Evaluation of the Biology GN MicroPlate system for identification of some plant-pathogenic bacteria /
[ Jones, J. B.;A. R. Chase;G. K. Harris ] / Plant Dis.   DOI   ScienceOn
3 /
[ Khush, G. S.;T. Kinoshita ] / Rice Biotechnology
4 Characterization of the Xanthomonas axonopodis pv. glycines Hrp pathogenicity island /
[ Kim, J. G.;B. K. Park;C. H. Jeon;E. Yoo;J. Oh;I. Hwang ] / J. Bacteriol.   DOI   ScienceOn
5 Report of committee on gene symbolization, nomenclature and linkage groups /
[ Kinoshita, T. ] / Rice Genet. Newsl.
6 Conservation of plasmid DNA sequences and pathovar identification of strains of Xanthomonas campestris /
[ Lazo, G. R.;D. W. Gabriel. ] / Phytopathology   DOI
7 Pathovar of Xanthomonas campestris are distinguishable by restriction fragment-length polymorphism /
[ Lazo, G. R.;D. W. Gabriel ] / Int. J. Syst. Bacteriol.   DOI
8 Identifying and mapping a new gene for bacterial blight resistance in rice based on RFLP markers /
[ Lin, X. H.;D. P. Zhang;Y. F. Xie;H. P. Gao;Q. Zhang ] / Phytopathology   DOI   ScienceOn
9 Use of Polymerase chain reaction for diagnosis of plant pathogenic bacteria /
[ Lopes, S. A.;K. E. Damann ] / Summa. Phytopathol.
10 Current status and future prospects of reseach on bacterial blight of rice /
[ Mew, T. W. ] / Annu. Rev. Phytopathol.   DOI   ScienceOn
11 Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting /
[ Meunier, J. R.;P. A. D. Grimount ] / Res. Microbiol.   DOI   ScienceOn
12 Rapid isolation of high molecular-weight plant DNA /
[ Murray, M. G.;W. F. Thompson ] / Nucleic Acids Res.   DOI   ScienceOn
13 Two novel type Ⅲ-secreted proteins of Xanthomonas compestris pv. vesicatoria are encoded within the hrp pathogenicity island /
[ Noel, L.;F. Thieme;D. Nennstiel;U. Bonas ] / J. Bacteriol.   DOI   ScienceOn
14 Specific detection of Pseudomonas syringae pv. phaseolicola DNA in bean seed by polymerase chain reaction-based amplification of a phaseolotoxin gene region /
[ Prosen D.;E. Hatziloukas;N. J. Panopoulas;N. W. Schaad ] / Phytopathology   DOI   ScienceOn
15 Rice bacterial blight status in Punjab, India /
[ Raina, G. L.;G. S. Sidhu;P. K. Saini ] / Rev. Plant. Pathol.
16 DNA probes for the detection of plant pathogenic bacteria /
[ Rasmussen, O. F.;J. C. Reeves ] / J. Biochnol.
17 /
[ Sambrook, J.;E. F. Fritsch;T. Maniatis ] / Molecular cloning:A laboratiry manual(2nd ed.)
18 A combined technique to detect Pseudomonas syringae pv. phaseolicola in bean seed extracts /
[ Schaad, N. W.;S. S. Cheong;S. Tamaki;E. Hartziloukas;N. J. Panopoulas ] / Phytopathology   DOI   ScienceOn
19 Different thermostable DNA polymerases may amplify different RAPD products /
[ Schierwater, B.;A. Ender ] / Nucleic Acid Res.   DOI   ScienceOn
20 Differentiation of Pseudomonas solanacearum Pseudomonas syzygii, Pseudomonas pickettii, and the bllod disease bacterium by partial 16S rRNA sequencing-construction of oligonucleotide primers for sensitive detection by polymerase chain reaction /
[ Seal, S. E.;L. A. Jackson;J. P. W. Young;M. J. Daniels ] / J. Gen. Microbial.   DOI   ScienceOn
21 Bacteria in apparently healthy Pinto beans /
[ Thomas W. D.Jr.;R. W. Graham ] / Phytopathology
22 Differntiation between Xanthomonas campestris pv. oryzae, Xanthomonas oryzae pv. oryzicola and the bacterial brown blotch pathogen on rice by numerical analysis of phenotypic features and protein gel electrophoregrams /
[ Vera Cruz C. M.;F. Gossele;K. Kersters;P. Segers;M. Van Den Mooter;J. Swings;J. De Ley ] / J. Gen. Microbiol.
23 Specific detection of Clavibacter Michiganense subsp. Michiganense by a homologous DNA probe /
[ Thompson E.;J. V. Leary;W. W. C. Chun ] / Phytopathology   DOI
24 DNA amplification fingerprinting of bacteria /
[ Bassam, B. J.;G. Caetano-Anolles;P. M. Gresshoff ] / Appl. Microbiol. Biotechnol.
25 /
[ Goodwin, P. H.;A. Nassuth ] / Methods in molecular biology and biotechnology
26 Plasmid-based hybridization probes for detection and identification of Xanthomonas campestris pv. citri /
[ Hartung, J. S. ] / Plant Dis.   DOI
27 hpaA mutants of Xanthomonas campestris pv. vesicatoria are affected in pathogenicity but retain the ability to induce host-specific hypersensitive reaction /
[ Huguet, E.;K. Hahn;K. Wengelnik;U. Bonas ] / Mol. Microbiol.   DOI   ScienceOn
28 Specific identification of Clavibacter Michiganense subsp. sepedonicum by DNA-hybridization probes /
[ Johansen, I. E.;O. F. Rasmussen;M. Heide ] / Phytopathology   DOI
29 Changes in race frequency of Xoo in response to rice cultivars planted in the Philippines /
[ Mew, T. W.;C. M. Vera Cruz;E. S. Medalla ] / Plant Dis.   DOI
30 Pathovar-specific monoclonal antibodies for Xanthomonas campestris pv. oryzae and for Xanthomonas oryzae pv. oryzicola /
[ Benedict, A. A.;A. M. Alvarez;J. Berstecky;W. Imanaka;C. Y. Mizumoto;L. W. Pollard;T. W. Mew;C. F. Gonzalez ] / Phytopathology   DOI
31 DNA amplification finterprinting using very short arbitrary oligonucleotide primers /
[ Caetano-Anolles, G.;B. J. Bassam;P. M. Gresshoff ] / Bio-Technology   DOI
32 Characterization of Xanthomonas campestris strains from aroids using physiological,pathological and fatty acid analyses /
[ Chase, A. R.;R. E. Stall;N. C. Hoodge;J. B. Jones ] / Phytopathology   DOI
33 Comparison of the genomes of two Xanthomonas pathogens with differing host specificities /
[ da Silva, A. C.;J. A. Ferro;F. C. Reinach;C. S. Farah;L. R. Furlan;R. B. Quaggio;C. B. Monteiro-Vitorello;M. A. Van Sluys;N. F. Almeida;L. M. Alves;A. M. do Amaral;M. C. Bertolini;L. E. Camargo;G. Camarotte;F. Cannavan;J. Car-dozo;F. Chambergo;L. P. Ciapina;R. M. Cicarelli;L. L. Coutinho;J. R. Cursino-Santos;H. El-Dorry;J. B. Faria;A. J. Ferreira;R. C. Ferreira;M. I. Ferro;E. F. Formighieri;M. c. Franco;C. C. Greggio;A. Gruber;A. M. Katsuyama;L. T. Kishi;R. P. leite;E. G. Lemos;m. V. Lemon;E. C. Locali;m. A. Machado;A. M. Madeira;N. M. Martinezaki;D. H. Moon;L. M. Moreira;m. T. novo;V. K. Okura;M. C. Oliveira;V. R. Oliveira;H. A. Pereira;A. Rossi;J. A. Sena;C. silva;R. F. de Souza;L. A. Spinola;m. A. takita;R. E. Tamura;E. C. teixeira;R. I. Tezza;M. trindade dos Santos;D. Truffi;S. M. Tsai;F. F. White;J. C. setubal;J. P. Kitajima ] / Nature   DOI   ScienceOn
34 Development and application of a plasmid DNA probe for detection of bacteria causing common bacterial blight of bean /
[ Gilbertson, R. L.;D. P. Maxwell;D. J. Hagedom;S. A. Leong ] / Phytopathology   DOI