• Research in Plant Disease
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• v.26 no.3
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• pp.179-182
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• 2020

In July 2018, a serious rust symptom was found throughout the fringe trees planted in Gangjin-gun, Korea. Yellow and brown spots were observed on the adaxial (topside) surface of the collected fringe tree leaves, and yellow color aecia were observed on the abaxial (underside) surface leaves. The size of aeciospore and urediniospores of JCK-KCFR1 strain were measured to 41.2 ㎛ (Φ) and 28.84 ㎛ (Φ) with a light microscope. Phylogenetic analysis of the small subunit rRNA, internal transcribed spacer, and large subunit rRNA region indicated that JCK-KCFR1 strain is novel species of the genus Puccinia and closely related to Puccinia kusanoi, which has been reported a rust pathogen on bamboo. In May 2019, rust symptoms were also discovered on the bamboo leaves planted around the fringe tree on Muwisa-ro, and their telia and teliospores were observed on the abaxial leaf surfaces of the bamboo with 100% sequence homology with the rust of the fringe tree. This is the first report that Puccinia sp. JCK-KCFR1 is a new species that requires both primary (fringe tree) and alternative (bamboo) host plants to complete its life cycle in Korea.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.618-623
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• 2015

The new white calla lily (Zantedeschia aethiopica) cultivar 'White Cutie' was bred at the National Institute of Horticultural & Herbal Science (NIHHS) in 2011. 'Childsiana' showing the multi-flowering characteristic and 'Wedding March' resistant to soft rot disease were artificially crossed in 2004. Of the progeny, 'White Cutie' was selected specifically for use in cut flower production after investigation over seven years (2005 to 2011) of genetic and phenotypic characteristics, resistance against soft rot, and customer preference regarding the culture vigor and post-harvest quality. 'White Cutie' was early flowering (85.6 days to flowering) with white flowers (RHS W155C), although it had a mid-sized flower in which spathe height and width were 8.6 cm and 8.7 cm, respectively. It was multi-flowering (6.2 flowers per plant) and produced a very high number of cormels (13.4 per plant). Furthermore, it was resistant to soft rot disease.

• Molecules and Cells
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• v.28 no.5
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• pp.431-439
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• 2009

Rice Oryza sativa accelerated cell death and resistance 1 (OsACDR1) encodes a putative Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK). We had previously reported upregulation of the OsACDR1 transcript by a range of environmental stimuli involved in eliciting defense-related pathways. Here we apply biochemical, gain and loss-of-function approaches to characterize OsACDR1 function in rice. The OsACDR1 protein showed autophosphorylation and possessed kinase activity. Rice plants overexpressing OsACDR1 exhibited spontaneous hypersensitive response (HR)-like lesions on leaves, upregulation of defense-related marker genes and accumulation of phenolic compounds and secondary metabolites (phytoalexins). These transgenic plants also acquired enhanced resistance to a fungal pathogen (Magnaporthe grisea) and showed inhibition of appressorial penetration on the leaf surface. In contrast, loss-of-function and RNA silenced OsACDR1 rice mutant plants showed downregulation of defense-related marker genes expressions and susceptibility to M. grisea. Furthermore, transient expression of an OsACDR1:GFP fusion protein in rice protoplast and onion epidermal cells revealed its localization to the nucleus. These results indicate that OsACDR1 plays an important role in the positive regulation of disease resistance in rice.

• Research in Plant Disease
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• v.10 no.3
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• pp.159-166
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• 2004

This study were carried out to identify pathogens and determine the seasonal occurrence and chemical control of safflower (Carthamus tinctorius) diseases from 2000 to 2002 in Gyengbuk province, Korea. Major diseases of safflower were, anthracnose caused by Colletotricum acutatum in open field, and gray mold by Botrytis cinerea in rain sheltered plastic house. Other diseases occurred were powdery mildew caused by Sphaerotheca fuliginea, collar rot by Sclerotium rolfsii, leaf spot by Alternaria carthami and A. alternata, rust by Puccinia carthami, root-rot and stem-rot by Phytophthora cactorum, root-rot and wilt by Fusarium oxysporum and damping-off by Pythium ultimum. Seasonal occurrence of anthracnose on safflower has begun from late April, and increased until harvesting, especially rapid increased after rainfall during stem elongation season that is from May to June. In open fields, maximum incidence of anthracnose was 67 ％ in late July. But in rain-sheltered plastic house, it was very low, about 5％ in July. Gray mold caused by Botrytis cinerea was most important disease in rain-sheltered plastic house cultivation. Maximum incidence of gray mold on floral head was 27.4％, whereas other diseases occurred below 1 ％. In the test of the chemical control of the safflower anthracnose, metiram WP, carbendazim$.$kasugamycin WP and iminoctadintris$.$thiram WP were the highest controlling chemicals. In chemical control of gray mold, iminoctadintris$.$thiram WP, fluazinam WP and iprodion WP showed highest controlling effects.

• Research in Plant Disease
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• v.23 no.1
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• pp.42-48
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• 2017

Chlorella is one of the microorganisms which can live autotrophically by their own photosynthesis. It was previously revealed that pre-treatment of Chlorella fusca caused a suppression of appressorium formation on the cucumber leaves after inoculation with Colletothrichum orbiculare. In this study, the ultrastructures of C. orbiculare on the cucumber leaves pretreated with C. fusca were observed using both scanning electron microscope (SEM) and transmission electron microscope (TEM). The SEM images revealed that most fungal conidia and hyphae were attached with lots of C. fusca cells. Also, the conidia could germinate but not form appressorium, which is necessary to penetrate into host tissue. These observations suggested that C. fusca adjoined to the fungus may play a role in suppression of the appressorium formation. On the other hand, the observations of TEM showed no remarkable cytological differences on the ultrastructures of the intracellular hyphae between in the pre-treated and untreated leaves. It seemed that the fungus could grow in the pre-treated plant tissues as in the untreated one. Based on these observations, it is suggested that the suppression of appressorium on the leaf surfaces by the C. fusca cells may be a main cause of the reduction of the anthracnose disease.

• Research in Plant Disease
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• v.13 no.1
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• pp.15-19
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• 2007

A wilt disease on the greenhouse-grown chrysanthemum occurred at Kumi, Masan and Busan, Korea in $2003{\sim}2005$. Symptoms of wilting, yellowing, and marginal burning started from lower leaves and progressed upward. Early symptoms often appeared on one side, involving only one part of the plant or one side of individual leaves or stems without a vascular discoloration. Vascular tissues of the infected leaf-base's discolored to brown. Fungal isolates obtained from discolored tissues were identified as Verticillium dahliae based on its cultural and morphological characteristics. The fungus showed whitish to creamy colony pattern with abundant dark brown to black elongated microsclerotia on PDA. Conidiophores were verticillately branched and conidia were ellipsoidal to sub-cylindrical shape, and measured as $2.5{\sim}8.8{\times}2.0{\sim}3.8{\mu}m$. Pathogenicity tests by root dipping resulted in similar symptoms observed in the naturally infected chrysanthemum in fields.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.210-218
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• 2015

Melon leaves showing yellowing symptoms were analyzed using electron microscopy and RT-PCR for major cucurbit-infecting-viruses (CMV, MNSV, CGMMV, SqMV, WMV, KGMMV, PRSV and ZYMV) reported in Korea, but these viruses were not detected. As the result of further analysis by next-generation sequencing (NGS), the virus was identified as Cucurbit aphid-borne yellows virus (CABYV), and then confirmed by RT-PCR using CABYV-specific primers. When photosynthetic capacity was measured based on chlorophyll fluorescence yield (ChlFY), the leaves of the diseased plants showed $4.09{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, which was one-third of the readings observed for unaffected normal plants ($12.36{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). The root functions of plants affected by leaf yellowing symptoms (LYS) was $0.28mg{\cdot}g^{-1}$, about half that measured for the normal unaffected plants ($0.48mg{\cdot}g^{-1}$). Cytological observations revealed that there were no morphological differences in the palisade parenchyma and mesophyll spongy cells of the leaves between the diseased and the normal plants. However, the same leaf cells of the affected plants contained more starch granules compared to those of the normal, unaffected plants. We conclude that the LYS of muskmelon is not merely a physiological disorder but a viral disease caused by CABYV and spread by aphids.

• Research in Plant Disease
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• v.14 no.3
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• pp.193-200
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• 2008

Blending of eggshell powder into soil as ratio of 1:5, 1:10, 1:15, 1:20, and 1:25 did not affect seed germination rates of several crops including Chinese cabbage. The blending increased pH of distilled water and decreased the viability of resting spores of Plasmodiophora hrassicae. The ratio of non-viable resting spores in eggshell-blending water was over five times higher than in distilled water of the same pH. Chinese cabbage (cv. 'Norangbom') grew more in eggshell-blended soil than in non-treated soil, but other crops grew less. Leaf numbers and above ground growth of Norangbom increased to around 150% and 470%, respectively, in soil blended with $1:20{\sim}1:15$ of eggshell powder. Even though the optimum sizes of eggshell powder were $0.8{\sim}2.0mm$ for growth and smaller than 0.4 mm for inhibition of clubroot disease of Chinese cabbage, there was no statistical difference among the sizes. Soil pH was above 8.0 in all eggshell treatments without any statistical difference among them. Eggshell powder blending to 1:20 showed lower control efficacy, 58.5%, than registered fungicide 'Hokanna (flusulfamide)', 78.5%. However, Chinese cabbage of that blending ratio recorded the highest growth among the treatments. Therefore, blending of eggshell powder into clubroot-contaminated soil may make culture of Chinese cabbage possible by growth-increasing, even though eggshell powder could not inhibit clubroot disease entirely.

• Research in Plant Disease
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• v.14 no.1
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• pp.68-70
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• 2008

Brown leaf spots on leaves of kiwifruit(Actinidia deliciosa) were observed at farmers' orchards in Suncheon and Goheung, Jeonnam Province, Korea in June, 2006. They developed to form dark brown ring spots and severely infected leaves resulted in defoliation during the growing season of kiwifruit. Alternaria sp. was isolated from the diseased leaves repeatedly and was identified as Alternaria alternata on the basis of its mycological characteristics on potato dextrose agar and its pathogenicity was confirmed by wound inoculation on healthy leaves of kiwifruit. A. alternata formed gray to dark sooty gray colony and produced numerous conidia on potato dextrose agar. The conidia, commonly in long chains of 5 or more produced on conidiophores, have $3{\sim}5$ transverse and $1{\sim}2$ longitudinal septa and mostly ovoid or obclavate in shape and were pale brown golden brown in color. The condia were $16.5{\sim}42.1{\times}6.7{\sim}19.5\;{\mu}m$ in size and conidiophores were $8.6{\sim}112.7\;{\mu}m$ in length. This is the first report on the brown ring spot on leaves of kiwifruit caused by A. alternata in Korea.

• Journal of Korean Society of Rural Planning
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• v.21 no.4
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• pp.35-43
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• 2015

The purpose of the research is to analyze damages of TYLCV (Tomato Yellow Leaf Curl Virus) in the context of climate changes and to find the spatial distribution of the damages and characteristics of regions. A TYLCV is generally known for a plant disease related to temperature. Its occurrence rate increases when temperature rises. This disease first occurred in 2008 and rapidly spread nationwide. Due to the spread of a TYLCV, a number of Tomato farms in Korea were damaged severely. To analyze damages of the pest in the context of climate changes, this research estimated production loss under the current situation and RCP scenarios. Additionally, Hot Spot Analysis, LISA, and Cluster analysis were conducted to find spatial distribution and properties of largely damaged regions under RCP scenarios. The results explained that additional production loss was estimated differently by regions with the same temperature rising scenario. Also, largely damaged regions are spatially clustered and factors causing large damages were different across regional cluster groups. It means that certain regions can be damaged more than others by diseases and pests. Furthermore, pest management policy should reflect the properties of each region such as climate conditions, cultivate environment and production technologies. The findings from this research can be utilized for developing rural management plans and pest protection policies.