Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Rust Disease on Fringe Tree by Puccinia sp. and Its Alternative Host

Puccinia sp.에 의한 이팝나무 잎녹병 발생 및 중간기주 보고

  • Yu, Nan Hee (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Park, Ae Ran (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Yoon, Hyeokjun (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Son, Youn Kyoung (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Lee, Byoung-Hee (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Kim, Jin-Cheol (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University)
  • 유난희 (전남대학교 농화학과) ;
  • 박애란 (전남대학교 농화학과) ;
  • 윤혁준 (국립생물자원관 유용자원분석과) ;
  • 손연경 (국립생물자원관 유용자원분석과) ;
  • 이병희 (국립생물자원관 유용자원분석과) ;
  • 김진철 (전남대학교 농화학과)
  • Received : 2020.07.21
  • Accepted : 2020.08.31
  • Published : 2020.09.30
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Abstract

In July 2018, a serious rust symptom was found throughout the fringe trees planted in Gangjin-gun, Korea. Yellow and brown spots were observed on the adaxial (topside) surface of the collected fringe tree leaves, and yellow color aecia were observed on the abaxial (underside) surface leaves. The size of aeciospore and urediniospores of JCK-KCFR1 strain were measured to 41.2 ㎛ (Φ) and 28.84 ㎛ (Φ) with a light microscope. Phylogenetic analysis of the small subunit rRNA, internal transcribed spacer, and large subunit rRNA region indicated that JCK-KCFR1 strain is novel species of the genus Puccinia and closely related to Puccinia kusanoi, which has been reported a rust pathogen on bamboo. In May 2019, rust symptoms were also discovered on the bamboo leaves planted around the fringe tree on Muwisa-ro, and their telia and teliospores were observed on the abaxial leaf surfaces of the bamboo with 100% sequence homology with the rust of the fringe tree. This is the first report that Puccinia sp. JCK-KCFR1 is a new species that requires both primary (fringe tree) and alternative (bamboo) host plants to complete its life cycle in Korea.

2018년 7월 전라남도 강진군의 가로수 전체 이팝나무에 심각한 녹병증상이 발견되어 잎을 채집하였다. 채집한 이팝나무 잎 앞면에서 황색 및 갈색의 병반과 잎 뒷면에서 황색의 녹포자기가 관찰되었고, 현미경아래에서 41.2 ㎛ 크기의 구형 녹포자와 28.84 ㎛ 크기의 구형 하포자가 확인되었다. 이팝나무 녹병균의 부분 small subunit rRNA, internal transcribed spacer (ITS) 1, 5.8S rRNA, ITS2, 부분 large subunit rRNA 염기서열을 이용하여 계통학적 유연관계를 분석한 결과, 대나무 녹병균으로 보고 되어져 있는 Puccinia kusanoi와 가까운 근연관계를 가지고 있었다. 또한 2019년 5월 무위사로 이팝나무 주변에 식재된 대나무에서도 녹병 병징이 관찰되었고, 잎 뒷면에서 동포자퇴와 동포자를 확인하였으며, 대나무에서 채집한 동포자의 염기서열 분석 결과, 이팝나무 녹병균과 100% 일치하였다. 본 논문은 대나무 녹병균으로 알려진 P. kusanoi와 근연관계가 가까운 새로운 종 Puccinia sp. JCK-KCFR1 strain (MT729824)가 국내에서 이팝나무와 대나무를 기주교대하며 녹병을 발생시키는 것을 처음으로 보고한다.

Keywords

References

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