• 한국환경복원기술학회지
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• 제10권5호
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• pp.67-75
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• 2007

The purpose of this study was to pelletize to calendula and salvia seeds with peat moss as basic material for the efficient greening and vegetating of slopes and damaged areas. Also the pelletizing was compressed by spherical types that mixed basic fertilizer of N.;300 mg/l, P.;200 mg/l, K.;400 mg/g and plant growth regulator of A and NAA each 300PPM. Soil and soil surface seeding methods were researched to find the growing state of germination percent, germination date, germination force, length of leaf, number of leaf, width of leaf, length of plant, and etc. Comparing with controlled pelletizing, peat moss and +GA pelletizing treatments resulted in higher from two to three times as following growing states : length of leaves, number of leaves, width of leaves, length of plants, length of roots, fresh weights, and ratio of germination. Especially the two treatments above showed four more times effects than the +NNA treatment. Also their germinating date germinating force were earlier and stronger. The surface seeding method was superior to soil seeding.

• The Plant Pathology Journal
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• 제39권6호
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• pp.566-574
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• 2023

The aim of this study was to investigate the regulation of lantipeptide production in Streptomyces globisporus SP6C4, which produces the novel antifungal lantipeptides conprimycin and grisin, and to identify the role of cytochrome P450 (P450) in tis regulation. To investigate the regulation of lantipeptide production, we created gene deletion mutants, including ΔP450, ΔtsrD, ΔlanM, ΔP450ΔtsrD, and ΔP450ΔlanM. These mutants were characterized in terms of their morphology, sporulation, attachment, and antifungal activity against Fusarium oxysporum. The gene deletion mutants showed distinct characteristics compared to the wild-type strain. Among them, the ΔP450ΔlanM double mutant exhibited a recovery of antifungal activity against F. oxysporum, indicating that P450 plays a significant role in regulating lantipeptide production in S. globisporus SP6C4. Our findings highlight the significant role of P450 in the regulation of lantipeptide production and morphological processes in S. globisporus. The results suggest a potential link between P450-mediated metabolic pathways and the regulation of growth and secondary metabolism in SP6C4, thereby highlighting P450 as a putative target for the development of new antifungal agents.

• 한국약용작물학회지
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• 제22권3호
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• pp.231-236
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• 2014

This experiment was carried out to establish the optimum cutting time, plant growth regulator, and bed-soil for rooting by greenwood cutting of Schisandra chinensis. Approximately 7 cm long-shoots of greenwood cuttings were transplanted by various cutting times, plant growth regulators, and bed-soils in the plastic-film house. The rooting rate of greenwood cutting was 12.5% in the April 5th, 73.5% in the May 8th, and 75.5% in the August 5th. The number and length of primary root in greenwood cutting were more in the early May than those in the early August. For mass propagation of Schisandra chinensis. using greenwood cutting, shoots were treated with plant growth regulators on May to increase rooting rate. Rooting rate was 100% with IAA $50mg/{\ell}$, 92.9% with NAA $100mg/{\ell}$, and NAA $1,000mg/{\ell}$, for 60 min. To select effective media for rooting, various medias for bed-soil were treated by single and mixture form for 100 days after cutting. Rooting rate was 91.8% in the single treatment of peat moss or decomposition of granite soil, and this result was better than those in other treatment. The treatment by 1 : 1 mixture of peat moss and horticulture bed soil was rooting with 94.0% best rooting rate.

• 한국약용작물학회지
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• 제10권5호
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• pp.344-352
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• 2002

본 시험은 국내 재래종 정선시호와 일본에서 도입된 삼도시호를 공시하여 시호의 품질 향상을 위해 식물생장조절제 처리가 시호의 생육 및 saikosaponin 함량에 미치는 요인들을 구명하여 얻어진 결과를 요약하면 다음과 같다. 1. 정선시호는 삼도시호보다 분지수는 적었지만 경수는 많았고, 근경이 굵으며 생근중과 건근중은 무거웠고 지근수도 많은 경향이었다. SSa 및 TSS 함량에서 정선시호가 삼도시호보다 높았고, SSc 함량에서는 정선시호가 삼도시호보다 적었다. 2. 식물생장조절제 처리시기 간에서는 6월에 처리한 것이 7월에 처리한 것보다 경수가 많았고 건근중에서는 유의적인 차이가 없었으나, 6월에 처리한 것이 7월에 처리한 것보다 SSd 함량이 높았다. 3. 식물생장조절제 처리에서 $GA_3$, 10, 50, 100 ppm, IAA 10, 50 ppm, kinetin 50 ppm 지상부 처리에서 지상부 생육을 촉진하였고, kinetin 50 ppm 처리구의 생근중과 건근중은 무처리구보다 무거웠으나 TSS 함량은 무처리구보다 낮았다. $GA_3$, 10 ppm, IAA 10 ppm처리에서 SSa, SSd 및 TSS 함량이 증가되었으며 TSS 함량은 $GA_3$, 50 및 100 ppm에서 높았다. 4. 무처리에 비하여 정선시호는 6월의 $GA_3$, 100 rpm, IAA 10 및 50 ppm, kinetin 10 ppm 처리구에서, 삼도시호는 6월의 $GA_3$, 10 ppm, IAA 10 및 100 ppm 처리구에서 생근중이나 근중은 차이가 없었지만 TSS 함량이 높아 시호생산에 가장 유리한 처리로 판단되었다.

• Molecules and Cells
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• 제19권3호
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• pp.342-349
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• 2005

Cytokinins are adenine derivatives that regulate numerous plant growth and developmental processes, including apical and floral meristem development, stem growth, leaf senescence, apical dominance, and stress tolerance. However, not much is known about how cytokinin biosynthesis and metabolism is regulated. We identified a novel Arabidopsis gene, ALL, encoding an aldolase-like enzyme that regulates cytokinin signaling. An Arabidopsis mutant, all-1D, in which ALL is activated by the nearby insertion of the 35S enhancer, exhibited extreme dwarfism with rolled, dark-green leaves and reduced apical dominance, symptomatic of cytokinin-overproducing mutants. Consistent with this, ARR4 and ARR5, two representative primary cytokinin-responsive genes, were significantly induced in all-1D. Whereas SHOOT MERISTEMLESS (STM) and KNAT1, which regulate meristem development, were also greatly induced, expression of REV and PHV that regulate lateral organ polarity was inhibited. ALL encodes an aldolase-like enzyme that belongs to the HpcH/HpaI aldolase family in prokaryotes and is down-regulated by exogenous cytokinin, possibly through a negative feedback pathway. We propose that ALL is involved in cytokinin biosynthesis or metabolism and acts as a positive regulator of cytokinin signaling during shoot apical meristem development and determination of lateral organ polarity.

• KSBB Journal
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• 제25권6호
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• pp.507-512
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• 2010

In this study, we investigated the ability of luteolin, a plant derived flavonoid on hepatocarcinoma cell growth using HepG2 cell culture system. We found that luteolin increased the Smac/DIABLO releases, a mitochondrial protein that potentiates apoptosis. Luteolin also induced either transcriptional activity or expression of PPAR-gamma, a target of cancer growth that PPAR-gamma agonist sensitizes to apoptosis in certain cancer types. To find the possible upstream target molecules of PPAR-gamma activated by luteolin treatment, we used compound C, a specific inhibitor of AMP-activated protein kinase. Pre-treatment of Compound C significantly restored the activation or expression of PPAR-gamma stimulated by luteolin. This result indicated that AMPK signaling might be involved in the activation or expression of PPAR-gamma signaling pathway stimulated by luteolin. Moreover, we also found that luteolin inhibited the insulin-stimulated Akt phosphorylation as well as AICAR, a specific AMPK activator. These results propose that luteolin significantly induces cancer cell death through modulating survival signal pathways such as PPAR-gamma and Akt. AMPK signaling pathway may be an upstream regulator for survival signal pathways such as PPAR-gamma and Akt stimulated by luteolin.

• 한국자원식물학회지
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• 제8권2호
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• pp.153-157
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• 1995

초피(椒皮)나무(Zanthoxylum piperitum DC.)의 embryogenic callus로부터 부정배발생(不定胚發生)에 있어 $NH_4NO_3,\;KNO_3$와 casein hydrolysate의 첨가(添加) 효과(效果)를 알아 보기위하여 본(本) 실험(實驗)을 실시(實施)하였던 바 그 결과(結果)는 다음과 같다. 1. 2,4-D 0.1mg/1가 첨가(添加)된 MT배지(培地)에 $NH_4NO_3$를 3300mg/1 첨가(添加)시킨 배지(培地)에서 부정배발생(不定胚發生)이 가장 양호(良好)하였다. 2. $KNO_3$를 3800mg/1 첨가(添加) 시킨 경우(境遇)에는 MT 기본배지(基本培地)와 2,4-D 0.1mg/1를 첨가(添加)시킨 배지(培地)에서 비교적(比較的) 양호(良好)한 부정배(不定胚)를 발생(發生)시키었다. 3. 2,4-D 0.1mg/1가 첨가(添加)된 MT 배지(培地)에 casein hydrolysate를 700mg/1 첨가(添加)시킨 경우(境遇)에 부정배(不定培) 발생(發生)이 양호(良好)하였으며 2,4-D가 첨가(添加)되지 않는 배지(培地)에서는 부정배(不定培) 발생(發生)이 저조(低調)하였다.

• Plant Biotechnology Reports
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• 제4권4호
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• pp.261-267
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• 2010

We describe culture conditions for a high-efficiency in vitro regeneration system of Papaver nudicaule through somatic embryogenesis and secondary somatic embryogenesis. The embryogenic callus induction rate was highest when petiole explants were cultured on Murashige and Skoog (MS) medium containing 1.0 mg $1^{-1}$ ${\alpha}$-naphthaleneacetic acid (NAA) and 0.1 mg $1^{-1}$ 6-benzyladenine (BA) (36.7%). When transferred to plant growth regulator (PGR)-free medium, 430 somatic embryos formed asynchronously from 90 mg of embryogenic callus in each 100-ml flask. Early-stage somatic embryos were transferred to MS medium containing 1.0 mg $1^{-1}$ BA and 1.0 mg $1^{-1}$ NAA to germinate at high frequency (97.6%). One-third-strength MS medium with 1.0% sucrose and 1.0 mg $1^{-1}$ $GA_3$ had the highest frequency of plantlet conversion from somatic embryos (91.2%). Over 90% of regenerated plantlets were successfully acclimated in the greenhouse. Secondary somatic embryos were frequently induced directly when the excised hypocotyls of the primary somatic embryos were cultured on MS medium without PGRs. Sucrose concentration significantly affected the induction of secondary embryos. The highest induction rate (89.5) and number of secondary somatic embryos per explant (9.3) were obtained by 1% sucrose. Most secondary embryos (87.2-94.3%) developed into the cotyledonary stage on induction medium. All cotyledonary secondary embryos were converted into plantlets both in liquid and on semisolid 1/3-strength MS medium with 1.0% sucrose.

• 한국자원식물학회지
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• 제19권2호
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• pp.315-322
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• 2006

로즈마리(Rosmarinus officinalis)와 라벤더(Lavandura spica L.)의 잎 절편으로부터 식물생장조절물질의 첨가에 따른 캘러스 유기율, 신초 및 뿌리의 분화율을 조사한 결과와 유기된 캘러스의 항산화 활성은 다음과 같다. 로즈마리의 캘러스 유기율은 2,4-D의 모든 처리구에서 양호 하였고, 특히 배양 90일째 2,4-D 1.0mg/L처리구에서 94%의 유기율로 가장 좋은 반응을 보였다. 로즈마리의 항산화 활성은 식물생장조절물질에 따라 다른 경향을 보였고, 2,4-D 0.1mg/L를 첨가하여 배양된 캘러스에서 $43{\mu}g(RC_{50})$으로 가장 높았으며, 2,4-D의 농도가 낮을 수 록 항산화 활성이 증가됨을 알 수 있었다. 또한 NAA, BA는 2,4-D와 반대로 농도가 증가함에 따라 항산화 활성이 증가됨을 알 수 있었다. 라벤더의 캘러스 유기는 2,4-D, NAA 및 BA의 모든 처리구에서 양호 하였으며, 로즈마리와 동일하게 2,4-D 1.0mg/L 처리구에서 98%의 유기율로 가장 양호하였다. 뿌리의 형성은 NAA 1.0mg/L을 첨가하였을 때 85%의 분화율로 가장 양호 하였다. 라벤더의 항산화 활성은 잎의 경우 $69.5{\mu}g(RC_{50})$으로 낮은 항산화 활성을 보였지만 2,4-D 0.1mg/L을 첨가하여 배양된 캘러스에서 $37.6{\mu}g(RC_{50})$으로 잎 보다 2배 정도 강한 항산화 활성을 보였으며, BA의 모든 처리구에서도 잎 보다 강한 항산화 활성을 보였다.

• Journal of Plant Biotechnology
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• 제49권1호
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• pp.82-89
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• 2022

정금나무(Vaccinium oldhamii)의 정아를 포함한 줄기를 재료로 다신초 유도 및 기외발근을 통해 완전한 식물체를 효과적으로 재분화시킬 수 있었다. 정아를 포함한 줄기로부터 다신초 유도는 2.0 mg/L 2-iP에서 유도율 100.0%, 절편체 당 다신초 유도수 7.4개로 나타났으며, 특히 신초 길이는 평균 51.7 mm로 다른 사이토키닌(zeatin, BA 또는 TDZ) 처리구에 비해 가장 높게 나타났다. 증식된 줄기로부터 기내발근은 0.5 mg/L IBA 첨가에서 절편체 당 2.4개의 뿌리를 생산하였고, 뿌리 길이는 평균 17.6 mm로 가장 우수한 결과를 보였다. 상토를 이용한 기외발근의 경우 1.0 mg/L IBA와 탈크(talc) 혼합용액 처리 시 평균 발근율은 80.0%로 기내발근에 비해 더 양호한 것으로 나타났다.