Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Callus induction and high-efficiency plant regeneration via somatic embryogenesis in Papaver nudicaule L., an ornamental medicinal plant

  • Yang, Jing Li (Key Laboratory of Forest Improvement and Biotechnology, Ministry of Education, Northeast Forestry University) ;
  • Zhao, Bo (Pharmacy College, Heilongjiang University of Traditional Chinese Medicine) ;
  • Seong, Eun-Soo (Bioherb Research Institute, Kangwon National University) ;
  • Kim, Myong-Jo (Bioherb Research Institute, Kangwon National University) ;
  • Kang, Won-Hee (Department of Horticulture, Kangwon National University) ;
  • Kim, Na-Young (Food Service Cuisine, Songho College) ;
  • Yu, Chang-Yeon (Bioherb Research Institute, Kangwon National University) ;
  • Li, Cheng Hao (Key Laboratory of Forest Improvement and Biotechnology, Ministry of Education, Northeast Forestry University)
  • Received : 2009.10.12
  • Accepted : 2010.07.02
  • Published : 2010.12.30
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Abstract

We describe culture conditions for a high-efficiency in vitro regeneration system of Papaver nudicaule through somatic embryogenesis and secondary somatic embryogenesis. The embryogenic callus induction rate was highest when petiole explants were cultured on Murashige and Skoog (MS) medium containing 1.0 mg $1^{-1}$ ${\alpha}$-naphthaleneacetic acid (NAA) and 0.1 mg $1^{-1}$ 6-benzyladenine (BA) (36.7%). When transferred to plant growth regulator (PGR)-free medium, 430 somatic embryos formed asynchronously from 90 mg of embryogenic callus in each 100-ml flask. Early-stage somatic embryos were transferred to MS medium containing 1.0 mg $1^{-1}$ BA and 1.0 mg $1^{-1}$ NAA to germinate at high frequency (97.6%). One-third-strength MS medium with 1.0% sucrose and 1.0 mg $1^{-1}$ $GA_3$ had the highest frequency of plantlet conversion from somatic embryos (91.2%). Over 90% of regenerated plantlets were successfully acclimated in the greenhouse. Secondary somatic embryos were frequently induced directly when the excised hypocotyls of the primary somatic embryos were cultured on MS medium without PGRs. Sucrose concentration significantly affected the induction of secondary embryos. The highest induction rate (89.5) and number of secondary somatic embryos per explant (9.3) were obtained by 1% sucrose. Most secondary embryos (87.2-94.3%) developed into the cotyledonary stage on induction medium. All cotyledonary secondary embryos were converted into plantlets both in liquid and on semisolid 1/3-strength MS medium with 1.0% sucrose.

Keywords

References

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