• 한국약용작물학회지
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• 제12권2호
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• pp.129-134
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• 2004

Optimal culture conditions for efficient in vitro propagation and polysaccharide production of Orostachys japonicus were established. The highest growth yield was achieved in 1/2 MS medium, while the lowest growth yield was obtained in 4 MS medium. The patterns of polysaccharide formation were a little similar in all cases, but on MB5 medium, the po]ysaccharide contents of plant were higher than others. Among the modified nitrate levels, effective growth level were obtained in 1/4 N and 1/2 N. High contents of polysaccharide were obtained in 4 N. Different concentration of potassium and calcium did not improve the growth and polysaccharide production. The micropropagated shoots were successfully acclimatized artificial soils.

• KSBB Journal
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• 제8권1호
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• pp.55-61
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• 1993

Ginkgo biloba의 세포배양을 통하여 세포성장과 flavonol glycosides의 생성에 영향을 미치는 여러 조건들을 연구하였다. 다양한 지역으로부터 유도된 세포주들의 세포성장과 flavonol glycosides의 생합성 능력은 서로 차이가 있었다. 이들의 캘러스 및 현탁 세포는 배지조건과 배양조건에 따라 성장 및 f1avonol glycosides 생합성에 많은 영향을 받았었다. 특히 배양조건 중 빛의 영향은 현저하여 명조건에서의 f1avonol glycosides의 생성은 암조건보다 10배 이상 증가함을 알 수 있었다.

• 한국대기환경학회지
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• 제2권1호
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• pp.11-21
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• 1986

The study was performed to investigate the effects of gaseous imission of sulfur dioxide and hydrogen fluoride on the growth of rice plant under stressed field conditions. The plants were cultivated in normal paddy fields where are 88 industrial plants operating with 285 smoke stacks emitting pollutants. There has been a number of reported studies (1, 3, 11, 19, 20) which deal with rice plant damages by air pollution under a simulated exposure experimental condition. Furthermore, these experiments were conducted to examine effects of a single pollutant on the plant. Furthermore, these experiments were conducted to examine effects of a single pollutant on the plant. In korea, however, there is no study reported in literature with respect to the in-situ dose-response relationship between rice pant reduction in yields and air pollution. This study is specifically dealt with multiple effects of sulfur dioxde and hydrogen fluoride on various plant growth indicators such as leaf damage, culm height, weight of grain, panicles per hill, spikelets per panicle and percent fertility.It appears that there is a good correlation between ambient concentrations of sulfur oxides and sulfur contents found in leaves with an average correlation coefficient of 0.868 within a 1% significance level. It is interesting to note that a better multiple correlation was found between percent leaf damage and sulfur and fluoride contentd found in leaf with a significance of 1% level. The yearly correlation coefficient ranges from 0.963 to 0.987 with an average being 0.971. It is, therefore, believed that a percent leaf damage may serve as a single indicator of pollutional damages to rice plant cultivating in fields. Regarding other factors to the diminution of rice plant growth in polluted atmosphere, it appears that a significant correlation to culm length and dry weight of grain with a 1% significance level whereas T/R ratio has a good correlation with lead damage within 5% significance level. An evaluation of data observed has demonstrated that both panicles per hill and percent fertility are significantly affected by air pollutants. As expected, hydrogen fluoride has more effects than sulfur oxide. It is, however, interesting to note that spikelets per panicles has slightly been affected while no indication of effects on 1000-grain-weight has been observed. This may lead to a conclusion that a reduction in yield of rice under polluted field conditions may have more been caused by the diminution of panicles per hill and percent fertility rather than by the diminution of spikelets per panicle and grain weight.

• 한국자원식물학회지
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• 제28권2호
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• pp.243-252
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• 2015

본 연구는 제주특산식물인 좀향유의 현지 내･외 보존을 위한 기초 연구로서 증식체계를 마련하고 전처리제 처리에 따른 발근 특성과 시비에 따른 유묘의 생장 효과를 등을 알아보았다. 온실과 밀폐상의 온습도 조사결과 평균 온도는 비슷하였으나 공중 습도는 온실보다 밀폐상이 높은 습도를 유지하였으며, 좀향유 삽목 후 온실은 10일, 밀폐상은 7일만에 최초 발근을 확인하여 밀폐상의 빠른 발근율을 보였으며 온실과 밀폐상에서의 생존율, 발근율, 뿌리수 등은 근소하게 밀폐상이 높게 조사되어 온실보다 밀폐상이 효과적이었다. 발근억제물질 제거를 위한 전처리제 처리는 AgNO₃ 77.5%를 제외한 다른 처리구에서 95% 이상의 높은 발근율로 조사되어 좀향유의 경우 발근억제 물질은 적은 것으로 판단된다. 유묘의 용기별 질소 시비량 및 Osmocote 시비시험 결과 시비량과 용기의 조건에 따라 생존율과 생장 등에 차이를 보였다. 배양판에서 완효성 비료인 Osmocote를 시비한 결과 생존율, 지상부, 지하부 생장 등이 가장 양호하여 좀향유의 유묘 증식을 위해서는 Osmocote 시비가 바람직 할 것으로 판단된다.

• 한국미생물·생명공학회지
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• 제51권4호
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• pp.484-499
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• 2023

Plant growth-promoting (PGP) bacteria can be used as bioresources to enhance phytoremediation through their PGP traits and pollutant removal capacity. In this study, 49 rhizobacteria were primarily isolated from the rhizosphere of tall fescue grown in diesel- and heavy metal-contaminated soil. Their biosurfactant production, phosphate (P) solubilization, and siderophore production were qualitatively and quantitatively evaluated to identify superior PGP bacteria. The optimal conditions for the growth of PGP bacteria and the stability of their PGP traits were a temperature of 35℃, a pH of 7, and 2 days of cultivation time. Four superior PGP bacteria (Pseudomonas sp. NL3, Bacillus sp. NL6, Bacillus sp. LBY14, and Priestia sp. TSY6) were finally selected. Pseudomonas sp. NL3 exhibited superior biosurfactant production and P solubilization. Bacillus sp. NL6 showed the highest P solubilization and superior production of biosurfactants and siderophores. Bacillus sp. LBY14 offered the best siderophore production and impressive P solubilization. Priestia sp. TSY6 had superior capacity for all three PGP traits. Through their secretion of beneficial PGP metabolites, the four bacteria isolated in this study have the potential for use in the phytoremediation of contaminated soil.

• 한국자원식물학회지
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• 제19권4호
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• pp.530-536
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• 2006

본 연구는 한라산의 대표적인 극지성 고산식물인 시로미의 현지내외 보존을 위한 기초 연구로서 증식체계를 마련하고 저지대에서 생장특성을 밝히고자 삽목조건 및 초기생육 특성 등을 알아보았다. 숙지삽시 식물호르몬의 처리나 상토의 조건에 따라 발근이나 생장 등이 다소 차이를 보였다. NAA 100 mg/l 또는 500 mg/l를 처리한 경우 생존율, 발근율 및 뿌리의 생장 등이 가장 양호할 뿐만 아니라 뿌리의 수도 많고 신초생장도 비교적 양호하여 시로미의 삽목증식을 위한 적정한 처리인 것으로 나타났다. 시로미 삽목묘를 노지에 옮겨서 키웠을 때에는 2개월 후부터 고사가 이루어지기 시작하여 7개월 후에는 26.7%가 고사한 것으로 나타났다. 이에 반해 차광처리를 하면 고사율이 낮아 $91.1{\sim}94.4%$가 생존하는 것으로 나타나 차광처리가 시로미의 생존율을 높이는데 효과적인 것으로 나타났다. 그리고 온실에서는 차광처리에 관계없이 생존율이 전체적으로 $95.6{\sim}97.8%$로 높게 나타났다. 그리고 생육장소 및 차광처리별 시로미의 평균 생장량은 노지와 온실에서 많은 차이를 보였다. 특히, 차광처리를 했을 때 상대적으로 높은 생장량을 보였는데 이는 차광처리가 상대습도를 높이고 광량을 낮추는 효과에 기인한 것으로 판단된다.

• Journal of Plant Biotechnology
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• 제30권3호
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• pp.293-299
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• 2003

The effects of low dosage ${\gamma}$-radiation on the cell growth and the formation of shikonin derivatives were investigated in callus cultures of Lithospermum erythrorhizon under different medium and light conditions. Gamma radiation significantly affected the cell growed and formation of shikonin derivatives, depending on the culture conditions. In the cell cultures grown on M-9 medium, 2Gy and 16Gy of ${\gamma}$-radiation increased the calli growth and the formation of shikonin derivatives, respectively under 16hr day light condition. When calli were cultured for 60 days in the dark after irradiation of ${\gamma}$-radiation, cell growth was increased at low dosage of 1Gy and 2Gy in LS medium containing BA 2mg/L and IAA 0.2mg/L. Interestingly, calli grown in M-9 medium by 2Gy irradiation for 60 days significantly stimulated the formation of shikonin derivatives(13.21mg/g cell fresh wt), which was approximately 6 times higher than untreated cells.

• Mycobiology
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• 제38권2호
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• pp.113-117
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• 2010

Trichoderma strains were evaluated under field conditions to assay their efficacy in suppressing Alternaria fruit rot disease and promoting chili plant growth. The experiment was conducted at the Botanical Garden, Rajshahi University, Bangladesh from July 2006 to March 2007. Application of Trichoderma harzianum IMI 392432 significantly (p = 0.05) suppressed the disease compared to Alternaria tenuis (T2) treatment and improved both growth and yield. The treatment T4 (T. harzianum IMI-392432 + A. tenuis) was most effective in reducing disease percentage (72.27%) compared to A. tenuis (T1) treatment. The highest seed germination rate (85.56%) and the highest growth and yield (12.5 g/plant) was also recorded in the same treatment (T4), followed by T5 (T. harzianum IMI-392433 + A. tenuis), T6 (T. harzianum IMI-392434 + A. tenuis), T2 (T. virens IMI-392430 + A. tenuis), and T3 (T. pseudokoningii IMI-392431 + A. tenuis) treatment, while single treatment with A. tenuis significantly decreased these values.

• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1577-1584
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• 2010

In total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the Yellow Sea, near the city of Incheon in the Republic of Korea. All of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (IAA), nitrogen fixation, phosphorus (P) and zinc (Zn) solubilization, thiosulfate ($S_2O_3$) oxidation, the production of ammonia ($NH_3$), and the production of extracellular hydrolytic enzymes such as protease, chitinase, pectinase, cellulase, and lipase under in vitro conditions. From the original 140 strains tested, on the basis of the latter tests for plant growth promotional activity, 36 were selected for further examination. These 36 halotolerant bacterial strains were then tested for 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity. Twenty-five of these were found to be positive, and to be exhibiting significantly varying levels of activity. 16S rRNA gene sequencing analyses of the 36 halotolerant strains showed that they belong to 10 different bacterial genera: Bacillus, Brevibacterium, Planococcus, Zhihengliuella, Halomonas, Exiguobacterium, Oceanimonas, Corynebacterium, Arthrobacter, and Micrococcus. Inoculation of the 14 halotolerant bacterial strains to ameliorate salt stress (150 mM NaCl) in canola plants produced an increase in root length of between 5.2% and 47.8%, and dry weight of between 16.2% and 43%, in comparison with the uninoculated positive controls. In particular, three of the bacteria, Brevibacterium epidermidis RS15, Micrococcus yunnanensis RS222, and Bacillus aryabhattai RS341, all showed more than 40% increase in root elongation and dry weight when compared with uninoculated salt-stressed canola seedlings. These results indicate that certain halotolerant bacteria, isolated from coastal soils, have a real potential to enhance plant growth under saline stress, through the reduction of ethylene production via ACC deaminase activity.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1622-1628
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• 2006

Bacteria from the Methylobacterium genus, called pink-pigmented facultative methylotrophic bacteria (PPFMs), are common inhabitants of plants, potentially dominating the phyllosphere population, and are also encountered in the rhizosphere, seeds, and other parts of plants, being versatile in nature. The consistent success of the Methylobacterium plant association relies on methylotrophy, the ability to utilize the one-carbon compound methanol emitted by plants. However, the efficiency of Methylobacterium in plant growth promotion could be better exploited and thus has attracted increasing interest in recent years. Accordingly, the present study investigated the inoculation effects of Methylobacterium sp. strains CBMB20 and CBMB 110 on seed imbibition to tomato and red pepper on the growth and accumulation of phytohormone levels under gnotobiotic conditions. Seeds treated with the Methylobacterium strains showed a significant increase in root length when compared with either the uninoculated control or Methylobacterium extorquens $miaA^-$ knockout mutanttreated seeds. Extracts of the plant samples were used for indole-3-acetic acid (IAA), trans-zeatin riboside (t-ZR), and dihydrozeatin riboside (DHZR) assays by immunoanalysis. The treatment with Methylobacterium sp. CBMB20 or CBMB 110 produced significant increases in the accumulation of IAA and the cytokinins t-ZR and DHZR in the red pepper extracts, whereas no IAA was detected in the tomato extracts, although the cytokinin concentrations were significantly increased. Therefore, this study proved that the versatility of Methylobacterium as a plant-growth promoting bacteria could be better exploited.