Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of Low Dose Gamma Radiation on the Formation of Shikonin Derivatives on Callus Cultures of Lithospermum erythrorhizon

지치(Lithospermum erythrorhizon S.)의 캘러스배양에서 Shikonin 유도체 생산에 미치는 저선량γ선의 효과

  • Hwang, Hye-Yeon (Department of Horticulture, Chungnam National University) ;
  • Kim, Jae-Sung (Radiation Application Research Team, Korea Atomic Energy Research Institute) ;
  • Lee, Young-Bok (Department of Horticulture, Chungnam National University)
  • 황혜연 (충남대학교 원예학과) ;
  • 김재성 (한국원자력연구소 방사선 이용연구부) ;
  • 이영복 (충남대학교 원예학과)
  • Published : 2003.09.01
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Abstract

The effects of low dosage ${\gamma}$-radiation on the cell growth and the formation of shikonin derivatives were investigated in callus cultures of Lithospermum erythrorhizon under different medium and light conditions. Gamma radiation significantly affected the cell growed and formation of shikonin derivatives, depending on the culture conditions. In the cell cultures grown on M-9 medium, 2Gy and 16Gy of ${\gamma}$-radiation increased the calli growth and the formation of shikonin derivatives, respectively under 16hr day light condition. When calli were cultured for 60 days in the dark after irradiation of ${\gamma}$-radiation, cell growth was increased at low dosage of 1Gy and 2Gy in LS medium containing BA 2mg/L and IAA 0.2mg/L. Interestingly, calli grown in M-9 medium by 2Gy irradiation for 60 days significantly stimulated the formation of shikonin derivatives(13.21mg/g cell fresh wt), which was approximately 6 times higher than untreated cells.

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References

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