• The Plant Pathology Journal
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• v.26 no.4
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• pp.402-408
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• 2010

Plant matrix metalloproteases (MMPs) are a family of apoplastic metalloproteases closely related to human matrilysins. Up-regulation of Nicotiana benthamiana matrix metalloprotease 1 (NMMP1) expression by treatment with pathogens, ethephon and aging indicates that the gene is related to plant defense and the aging process through ethylene signaling. NMMP1 expression was higher than in normal growth leaves following infection with an incompatible pathogen Pseudomonas syringae pv. tomato T1 or a compatible pathogen P. syringae pv. tabaci and in aged leaves. Transient overexpression of NMMP1 in N. benthamiana leaves lowered the growth of P. syringae pv. tabaci. However, NMMP1-silenced leaves showed increased growth of P. syringae pv. tabaci. These data strongly suggest that NMMP1 in N. benthamiana is a defense related gene, which is positively regulated by ethylene.

• Applied Biological Chemistry
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• v.41 no.1
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• pp.104-109
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• 1998

Methanol extracts from 84 Indian plant samples (50 species in 31 families) and 27 African plant samples (20 species in 12 families) in vitro were tested for their growth-inhibitory activities against Bifidobacterium bifidum, Bifidobacterium longum, Lactobacillus acidophilus, Clostridium perfringens, and Escherichia coli, using a paper disc agar diffusion method under $O_2-free$ conditions. The responses varied with bacterial strain, plant species and plant part. Extracts from Cymbopogon citratus whole plants, Ocimum basilicum whole plant, Madhuca indica flowers, and Aegle marmelos leaves among Indian plant samples moderately or strongly inhibited the growth of Cl, perfringens whereas moderate growth-inhibitory activity against E. coli was obtained from extract of Indian O. basilicum whole plants. These plant extracts did not affect the growth of the lactic acid forming bacteria tested. These results may be an indication of at least one of the pharmacological actions of these tropical plants.

• Mycobiology
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• v.40 no.4
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• pp.244-251
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• 2012

In vitro and greenhouse screening of seven rhizobacterial isolates, AB05, AB10, AB11, AB12, AB14, AB15 and AB17, was conducted to investigate the plant growth promoting activities and inhibition against anthracnose caused by Colletotrichum acutatum in pepper. According to identification based on 16S rDNA sequencing, the majority of the isolates are members of Bacillus and a single isolate belongs to the genus Paenibacillus. All seven bacterial isolates were capable of inhibiting C. acutatum to various degrees. The results primarily showed that antibiotic substances produced by the selected bacteria were effective and resulted in strong antifungal activity against the fungi. However, isolate AB15 was the most effective bacterial strain, with the potential to suppress more than 50% mycelial growth of C. acutatum in vitro. Moreover, antibiotics from Paenibacillus polymyxa (AB15) and volatile compounds from Bacillus subtilis (AB14) exerted efficient antagonistic activity against the pathogens in a dual culture assay. In vivo suppression activity of selected bacteria was also analyzed in a greenhouse with the reference to their prominent in vitro antagonism efficacy. Induced systemic resistance in pepper against C. acutatum was also observed under greenhouse conditions. Where, isolate AB15 was found to be the most effective bacterial strain at suppressing pepper anthracnose under greenhouse conditions. Moreover, four isolates, AB10, AB12, AB15, and AB17, were identified as the most effective growth promoting bacteria under greenhouse conditions, with AB17 inducing the greatest enhancement of pepper growth.

• Research in Plant Disease
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• v.22 no.4
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• pp.284-288
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• 2016

A plant growth promoting rhizobacterium, Bacillus vallismortis BS07M, was formulated as a clay pellet (CP) to evaluate its pepper growth promotion and induced resistance against various diseases under field and storage conditions. Peppers were grown in 50-hole tray containing potting mixture with CP in seedling raising stage, and then it was transplanted into a field. After transplanting, pepper plants treated with CP in seedling raising stage increased shoot growth and reduced disease severity caused by Phytophthora capsici in detached pepper leaves compared to untreated control. Moreover, treatment with CP in seedling raising stage increased fruit weight per plant; after harvesting, pepper fruits shown reduced diameter of lesions by Colletotrichum acutatum, and occurrance of soft rot in storage condition. These results indicated that CP could affect plant growth and induced resistance in pepper plants under field condition, and maintenance of fruit during storage.

• Microbiology and Biotechnology Letters
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• v.48 no.4
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• pp.399-422
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• 2020

Remediating soils contaminated with heavy metals due to urbanization and industrialization is very important not only for human health but also for ecosystem sustainability. Of the available remediation technologies for heavy metal-contaminated soils, phytoremediation is a relatively low-cost environment-friendly technology which preserves biodiversity and soil fertility. The application of plant growth-promoting bacteria (PGPB) during the phytoremediation of heavy metal-contaminated soils can enhance plant growth against heavy metal toxicity and increase heavy metal removal efficiency. In this study, the sources of heavy metals that have adverse effects on microorganisms, plants, and humans, and the plant growth-promoting traits of PGPB are addressed and the research trends of PGPB-assisted phytoremediation over the last 10 years are summarized. In addition, the effects of environmental factors and PGPB inoculation methods on the performance of PGPB-assisted phytoremediation are discussed. For the innovation of PGPB-assisted phytoremediation, it is necessary to understand the behavior of PGPB and the interactions among plant, PGPB, and indigenous microorganisms in the field.

• Korean Journal of Plant Resources
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• v.35 no.1
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• pp.29-35
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• 2022

Elsholtzia byeonsanensis is a Korean native plant of the Lamiaceae family, and was first introduced in the Korean Journal of Plant Taxonomy. This plant has a short plant length of 20-35 cm, with leathery and glossy leaves. The purple flowers bloom in November, so they are highly useful as potted or ground cover ornamental plants. In this study, tray cell size (128, 162, and 200-cell trays), soil composition [horticultural soil (HS): decomposed granite (DG) 1:1, 1:2, and 1:4 (v:v)], seeding rate (1, 2, and 3 seeds per cell) and additional fertilizer concentrations (0, 250, 500, and 1000 mg/L Hyponex) were tested to find out the optimum seedling growth conditions. As a result of the study, E. byeonsanensis growth increased proportionally as the cell size of the tray increased. The growth was inhibited as the percentage of DG increased. As for the growth according to the seeding rate, plant height, plant width, leaf length, and leaf width decreased as the seeding rate increased, but there was no difference in the number of leaves, stem diameter, and root length. There was an increasing trend in seedling growth as the additional fertilizer concentration increased. Therefore, for producing E. byeonsanensis seedling, it is most effective to fill HS or HS:DG 1:1 in 128-cell trays, sow one seed per cell, and spray the seedling at 1000 mg/L for additional fertilizer.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.2
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• pp.78-85
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• 1993

This experiment was carried out to select for the varieties with high environmental adaptability in mountainous pastures of Taekwalyong area, and the evaluation was based on the data of varietal differences of dry matter production and yield components in Tall fescue cutting at various developmental stages in growth period of autumn. The results were summarized as follows; 1. The varieties with high number of tillers per plant(NT) showed a high dry weight of plant(DW) in all autumn cuttings. These varieties(Barce1 and Fuego) may be more adaptable to mountainous pastures of Taekwalyon area than other varieties. 2. The number of tillers per plant(NT) was significantly positive correlated with dry weight of plant (DW), but dry weight of tiller(WT) was not significantly correlated with dry weight of plant(DW) in all autumn cuttings. 3. The multiple regression equations of autumn cutting stages with mean dry weight of plant(DW) and number of tillers per plant(NT) can be used to estimate the cutting time to obtain maximum mean dry weight of plant(DW) and the highest number of tillers per plant(NT). The maximum mean dry weight of plant(DW) and the highest number of tillers per plant(NT) was estimated at the time of 21 Oct. and 26 Oct, respectively. 4. Dead leaves tended to increase greatly after the 2nd cutting(l4 Oct.) in growth period of autumn. 5. The number of tillers per plant(NT) was an important yield component before tiller density achieved equilibrium, but dry weight of tiller(WT, including dead leaves) was of most significant as a yield determinant after maximum tiller density reached in growth period of autumn.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.153-160
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• 2004

Glehnia littoralis is known as an edible and medicinal plant using green loaves and mature roots of plant. In the present paper, the influence of plant growth regulators on callus induction and plant regeneration was investigated. Callus induction and regeneration occurred from leaf and petiole explants in Glehnia littoralis. Optimal condition of plant growth regulators for callus induction from leaf and petiole explants was MS basal medium supplemented with 2mg/L 2,4-D and 2mg/L BA. The frequency of callus induction was higher in petiole explant than leaf. When the callus was cultured on MS basal medium supplemented with 0∼1 mg/L IAA, 0∼1mg/L NAA and 0∼2mg/L BA for about 65 days, the most effective plant growth regulators on plant regeneration from callus were 1mg/L NAA and 2mg/L BA. The plantlets acclimatized successfully and grown in vermiculite matrix.

• The Korean Journal of Ecology
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• v.17 no.2
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• pp.149-157
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• 1994

To investigate the causes of distribution of Kummerowia striata (Thunb.) Schindl. mostly restricted on the roadside and reclaimed land, plant growth was analyzed under different of tread-pressures: $P_1$(10 times/day, $16.34Kg/cm^2$), $P_2$(20 times/day, $31.52 Kg/cm^2$), $P_3$(30 times/day, $40.79 kg/cm^2$) and the control $P_c$(0 times/day, $3.73 Kg/cm^2$). The matter production in the $P_1$decreased compared with that in the $P_c$, and those in the $P_2$and $P_3$ continuously decreased so that its length growth gradually decreased and, in particular, the growth of leaves and nodule was suppressed. The number of leaves and roots tended to increase in the $P_2$and $P_3$. The water contents of roots following the increase of tread-pressure increased continuously. Following the increase of tread-pressure, T/R ratio and C/F ratio tended to increase. Kummerowia striata showed the highest growth in the $P_c$ and showed 26% relative growth in the $P_1$compared with that in the $P_c$. This plant showed 8% relative growth in the $P_3$, which is excess to the tread-pressure $21Kg/cm^2$, the limit of growth in the plant. Because this plant grew continuously in the P$_3$, the tread-pressure is regarded as an important factor affecting the roadside distribution of this plant.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.1
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• pp.59-65
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• 2012

Plant growth-promoting effects of rhizobacterial inoculation obtained in pot experiments cannot always be dependably reproduced in fields. In this study, we investigated the effect of inoculation with Azospirillum brasilense and Methylobacterium oryzae, which have displayed growth promoting effects in several pot experiments, on growth and fruit yield of red pepper under field condition in a plastic-film house. Four rows spaced 90 cm apart were prepared after application of compost ($10Mg\;ha^{-1}$), and red pepper seedlings (Capsicum annum L., Nocgwang) were transplanted in each row with 40-cm space. Experimental treatments were consisted of A. brasilense CW903 inoculation, M. oryzae CBMB20 inoculation, and uninoculated control. Twelve plots, 10 plants per plot, were allotted to the three treatments with four replicates in a completely randomized design. At the time of transplanting, 50 mL of each inoculum ($1{\times}10^8cells\;mL^{-1}$) was introduced into root zone soil of each plant, and re-inoculated at 7 and 14 days after transplant. Plant growth and fruit yield were measured during the experiment. Both A. brasilense CW903 and M. oryzae CBMB20 could not promote growth of red pepper plants. All growth parameters measured were not significantly different among treatments. There were large variations in fruit yield recorded on plot basis, and no statistically significant differences were found among treatments. The failure to demonstrate the expected plant growth promoting effect of the inoculants is possibly due to various environmental factors, including weather and soil characteristics, reducing the possibility to express the potential of the inoculated bacterial strains.