Browse > Article
http://dx.doi.org/10.5423/PPJ.2010.26.4.402

NMMP1, a Matrix Metalloprotease in Nicotiana benthamiana Has a Role in Protection against Bacterial Infection  

Kang, So-Ra (School of Biotechnology, Yeungnam University)
Oh, Sang-Keun (Department of Plant Sciences, Seoul National University)
Kim, Jong-Joo (School of Biotechnology, Yeungnam University)
Choi, Do-Il (Department of Plant Sciences, Seoul National University)
Baek, Kwang-Hyun (School of Biotechnology, Yeungnam University)
Publication Information
The Plant Pathology Journal / v.26, no.4, 2010 , pp. 402-408 More about this Journal
Abstract
Plant matrix metalloproteases (MMPs) are a family of apoplastic metalloproteases closely related to human matrilysins. Up-regulation of Nicotiana benthamiana matrix metalloprotease 1 (NMMP1) expression by treatment with pathogens, ethephon and aging indicates that the gene is related to plant defense and the aging process through ethylene signaling. NMMP1 expression was higher than in normal growth leaves following infection with an incompatible pathogen Pseudomonas syringae pv. tomato T1 or a compatible pathogen P. syringae pv. tabaci and in aged leaves. Transient overexpression of NMMP1 in N. benthamiana leaves lowered the growth of P. syringae pv. tabaci. However, NMMP1-silenced leaves showed increased growth of P. syringae pv. tabaci. These data strongly suggest that NMMP1 in N. benthamiana is a defense related gene, which is positively regulated by ethylene.
Keywords
Defense; matrix metalloprotease; overexpression; pathogen; virus-induced gene silencing (VIGS);
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
연도 인용수 순위
1 Schiermeyer, A., Hartenstein, H., Mandal, M. K., Otte, B., Wahner, V. and Schillberg, S. 2009. A membrane-bound matrixmetalloproteinase from Nicotiana tabacum cv. BY-2 is induced by bacterial pathogens. BMC Plant Biol. 9:83.   DOI   ScienceOn
2 van der Hoorn, R. A. L. and Jones, J. D. 2004. The plant proteolytic machinery and its role in defence. Curr. Opin. Plant. Biol. 7:400-407.   DOI   ScienceOn
3 van der Hoorn R. A. L. 2008. Plant proteases: From phenotypes to molecular mechanisms. Annu. Rev. Plant Biol. 59:191-223.   DOI   ScienceOn
4 Kapila, J., De Rycke, R., Van Montagu, M. and Angenon, G.. 1997. An Agrobacterium-mediated transient gene expression system for intact leaves. Plant Sci. 122:101-108.   DOI   ScienceOn
5 Liu, Y., Dammann, C. and Bhattacharyya, M. K. 2001. The matrix metalloproteinase gene GmMMP2 is activated in response to pathogenic infections in soybean. Plant Physiol. 127:1788-1797.   DOI
6 Liu, Y., Schiff, M. and Dinesh-Kumar S. P. 2002. Virus-induced gene silencing in tomato. Plant J. 31:777-786.   DOI   ScienceOn
7 Maidment, J. M., Moore, D., Murphy, G. P., Murphy, G. and Clark, I. M. 1999. Matrix metalloproteinase homologues from Arabidopsis thaliana. J. Biol. Chem. 274:34706-34710.
8 McGeehan, G.., Burkhart, W., Anderegg, R., Becherer, J. D., Gillikin, J. W. and Graham, J. S. 1992. Sequencing and characterization of the soybean leaf metalloproteinase: Structural and functional similarity to the matrix metalloproteinase family. Plant Physiol. 99:1179-1183.   DOI   ScienceOn
9 Nagase, H. and Woessner, J. F. Jr. 1999. Matrix metalloproteinases. J. Biol. Chem. 274:21491-21494.   DOI
10 Pak, J. H., Liu, C. Y., Huangpu, J. and Graham, J. S. 1997. Construction and characterization of the soybean leaf metalloproteinase cDNA. FEBS Lett. 404:283-288.   DOI   ScienceOn
11 Chung, E., Seong, E., Kim, Y. C., Chung, E. J., Oh, S. K., Lee, S., Park, J. M., Joung, Y. H. and Choi, D. 2004. A method of high frequency virus-induced gene silencing in chili pepper (Capsicum annuum L. cv. Bukang). Mol. Cells 17:377-380.
12 Ratnaparkhe, S. M., Egertsdotter, E. M. U. and Flinn, B. S. 2009. Identification and characterization of a matrix metalloproteinase (Pta1-MMP) expressed during Loblolly pine (Pinus taeda) seed development, germination completion, and early seedling establishment. Planta 230:339-354.   DOI
13 Rawlings, N. D., Barrett, A. J. and Bateman, A. 2010. MEROPS: the peptidase database. Nucl. Acids Res. 38:D227-D233.   DOI   ScienceOn
14 Roberts, K. 1994. The plant extracellular matrix: in a new expansive mood. Curr. Opin. Cell Biol. 6:688-694.   DOI   ScienceOn
15 Coffeen, W. C. and Wolpert, T. J. 2004. Purification and characterization of serine proteases that exhibit caspase-like activity and are associated with programmed cell death in Avena sativa. Plant Cell 16:857-873.   DOI   ScienceOn
16 Combier, J. P., Vernié, T., De Billy, F., El Yahyaoui, F., Mathis, R. and Gamas, P. 2007. The MtMMPL1 early nodulin is a novel member of the matrix metalloendoproteinase family with a role in Medicago truncatula infection by Sinorhizobium meliloti. Plant Physiol. 144:703-716.   DOI   ScienceOn
17 Delorme, V. G., McCabe, P. F., Kim, D. J. and Leaver, C. J. 2007. A matrix metalloproteinase gene is expressed at the boundary of senescence and programmed cell death in cucumber. Plant Physiol. 123:917-927.   DOI
18 Dreher, K. A. and Callis, J. 2000. Ubiquitin, hormones and biotic stress in plants. Ann. Bot. 9:787-822.
19 Eisenhaber, B., Bork, P. and Eisenhaber, F. 1999. Prediction of potential GPI-modification sites in proprotein sequences. J. Mol. Biol. 292:741-758.   DOI   ScienceOn
20 Garcia-Lorenzo, M., Sjodin, A., Jansson, S. and Funk, C. 2006. Protease gene families in Populus and Arabidopsis. BMC Plant Biol. 6:30.   DOI
21 Golldack, D., Popova, O. V. and Dietz, K. J. 2002. Mutation of the matrix metalloproteinase At2-MMP inhibits growth and causes late flowering and early senescence in Arabidopsis. J. Biol. Chem. 277:5541-5547.   DOI   ScienceOn
22 Graham, I. A., Xiong, J. and Gillikin, J. W. 1991. Purification and developmental analysis of a metalloproteinase from the leaves of Glycine max. Plant Physiol. 97:786-792.   DOI   ScienceOn
23 Barrett, A. J., Rawlings, N. D. and Woessner, J. F. eds. 2004. Handbook of proteolytic enzymes. Elservier Academic Press, London.
24 Almeida, N. F., Yan, S. C., Lindeberg, M. D., Studholme, D. J., Schneider, D. J., Condon, B. F., Liu, H. J., Viana, C. J., Warren, A., Evans, C., Kemen, E., MacLean, D., Angot, A., Martin, G. B., Jones, J. D., Collmer, A., Setubal, J. C. and Vinatzer, B. A. 2009. A Draft genome sequence of Pseudomonas syringae pv. tomato T1 reveals a type III effector repertoire significantly divergent from that of Pseudomonas syringae pv. tomato DC3000. Mol. Plant-Microbe Interact. 22:52-62.   DOI   ScienceOn
25 An, G. 1987. Binary Ti vectors for plant transformation and promoter analysis. Meth. Enzymol. 153:292-305.   DOI
26 Baek, K.-H. and Choi, D. 2008. Roles of plant proteases in pathogen defense. Plant Pathol. J. 24:367-487.   DOI   ScienceOn