• 동의생리병리학회지
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• 제24권1호
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• pp.118-123
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• 2010

Piper nigrum Linne has been used spice as well as herbal medicine in worldwide and has function of anti-oxidant, anti-inflammation, anti-cancer, bioavailability of drugs and etc. In this study, anti-adipogenic activity of Piper nigrum Linne extract and its constituent piperine were investigated in 3T3-L1 differentiation. Adipogenic effects and lipid accumulation in 3T3-L1 was measured by RT-PCR and Oil Red O staining assays respectively in stimulation of Piper nigrum Linne extract and piperine. Piper nigrum Linne and piperine reduced lipid accumulation in 3T3-L1 differentiation and expression of genes associated with adipogenesis such as PPAR${\gamma}$, adipsin, SERBP-1c and LPL. All of taken, anti-adipogenic mechanism of Piper nigrum Linne and piperine were related with regulation of SREBP-1c and PPAR${\gamma}$ expression.

• Natural Product Sciences
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• 제25권3호
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• pp.255-260
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• 2019

Piper nigrum L. (Piperaceae), which is a well-known food seasoning, has been used as a traditional medicine for the treatment of vomiting, abdominal pain, diarrhea and anorexia in Korea, China and Japan. Methanol extract from the fruit of P. nigrum was successively partitioned as n-hexane, methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions. Among those fractions the ethyl acetate soluble fraction showed the most potent DPPH radical scavenging activity, and piperine was isolated from the ethyl acetate fraction. To know the antioxidant activity of piperine, we tested the activities of superoxide dismutase (SOD) and catalase together with oxidative stress tolerance and intracellular ROS level in Caenorhabditis elegans. To investigate whether piperine-mediated increased stress tolerance was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain including CF1553. Consequently, piperine enhanced SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, piperine-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.

• 한국응용곤충학회지
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• 제51권2호
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• pp.149-152
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• 2012

잎침지법을 이용하여 후추 열매 메탄올 추출물 및 분리된 화합물들의 배추좀나방(Plutellaxylostella) 유충에 대한 살충 활성을 검정하였다. 후추 열매 메탄올 추출물은 배추좀나방 유충에 대해 5.0과 2.5 mg/mL 농도에서 각각 100%와 97%의 살충 활성을 보였다. 후추 열매에서 유래된 화합물들의 배추좀나방에 대한 살충 활성을 조사한 결과, guineensine ($LC_{50}$=0.013 mg/mL)의 살충 활성이 가장 높았으며, 다음으로 retrofractamide A (0.020 mg/mL), pipercide (0.033 mg/mL) 그리고 pellitorine (0.046 mg/mL) 순이었다. Piperine의 반수치사농도는 0.5 mg/mL 이상이었다.

• 동의생리병리학회지
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• 제24권5호
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• pp.831-836
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• 2010

In addition to spice, black pepper (Piper nigrum Linne : PnL) has been used as herbal medicine because of its function in anti-oxidation, anti-inflammation, and anti-carcinogenesis. Recently, it has been reported that piperine, a component of PnL, inhibits adipocyte differentiation by repressing various adipogenic gene expressions. In this study, we determined whether piperine is a major constituent of PnL that confers the anti-adipogenic activity at whole genome level. Differentiation of 3T3-L1 pre-adipocytes was induced in presence of PnL extract or piperine. To compare genes that are regulated by PnL extract or piperine, we performed expression profiling using microarrays (Agilent Mouse 44k 4plex). RNA samples were labeled with Cy3 and Cy5, respectively. Labeled samples were hybridized to the microarrays. Results were filtered and cut off set p<0.05. Genes exhibiting significant differences in expression level were classified into Gene Ontology (GO)-based functional categories (http://www.geneontology.org) and KEGG (http://www.genome.jp/kegg/). Extract of PnL and its component piperine reduced lipid accumulation in 3T3-L1 cells during adipogenesis. Such anti-adipogenic activity appears to result from down-regulation of transcription factor genes involved in adipogenesis, and other genes involved in fatty acid synthesis, transport, triglyceride synthesis, and carbohydrate metabolism. These genome-wide studies lead to conclude that piperine, as a critical component of PnL, plays common role with PnL in anti-adipogenesis.

• Advances in Traditional Medicine
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• 제9권2호
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• pp.142-148
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• 2009

Anxiety disorders are one of the serious problems which need proper therapy devoid of side effects of presently available medicines. The present study evaluates the anxiolytic and sedative activity of leaf galls of Piper nigrum Linn. in Swiss Albino mice. The pet. ether, chloroform, ethyl acetate and ethanol extracts of leaf galls of Piper nigrum Linn were obtained by continuous soxhlet extraction. The prepared extracts were found to be safe up to 2000 mg/kg body weight of mice in the acute toxicity study. Each extract was assessed for anxiolytic activity in Swiss Albino mice by elevated plus Maze, open field test, rota rod test and phenobarbitone induced sleeping time test. In the Elevated Plus Maze test, the pet.ether extract and chloroform extract at a dose of 50 mg/kg b.w. orally, significantly (P < 0.01) increased the number of entries and time spent in open arm comparable with standard diazepam at the dose of 10 mg/kg. b.w. p.o. In the open field test, pet. ether extract (50 mg/kg b.w. p.o.) showed significant increase (P < 0.01) in ambulation and activity in the center. Chloroform extract (50 mg/kg b.w p.o.) was significant (P < 0.05) for both ambulation and center activity. Pet. ether extract (50 mg/kg b.w. p.o) also showed significant activity (P < 0.01) in rota rod test. All the results are comparable with standard diazepam at the dose of 1 mg /kg b.w, p.o. Moreover all the extracts showed significant (P < 0.01) increase in the phenobarbitone induced sleeping time among which pet.ether showed more prominent activity (36%) comparable with control. The results revealed that, the active pet.ether extract and chloroform extract of leaf galls of Piper nigrum Linn is worthwhile to develop the bioactive principle for anxiolytic activity.

• Advances in Traditional Medicine
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• 제3권1호
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• pp.8-17
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• 2003

Water extract (kwath) of six different widely used Ayurvedic medicinal plants were tested in mice for possible neuropharmacological efficacy. In the present experiments it was observed that a number of plant tested causes a significant level of Central Nervous System (CNS) depression, in that it significantly decreased the spontaneous Motor activity, and also lowered the exploratory behavior of the treated animals. Terminalia chebula (HAA), Terminalia bellerica (BHA), Emblica officinalis (AA), Piper longum 1. (PP). exhibited depressant action of on the CNS. Apart from them a mild to moderate degree of depression was evident as a consequence of administration of Zingiber officinale Rosc (SUT), Piper nigrum L. (MRC). However, none of the plant tested did not exhibit significant effects on pentobarbital induced narcosis, and this indicated that the sedating effects of the drug was not associated with the risk of fatal consequences on overdose.

• Journal of Plant Biology
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• 제32권1호
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• pp.11-21
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• 1989

Anatomical and histochemical changes taking place in Piper nigrum berries during their ripening are described. The important observations on the pericarp are the development of sclereids in the exocarp, a continuous band of oil cells in mesocarp and the wall thickening of the endocarpic cells. The mature seed with a single layer of seed coat, representing the innermost tegment layer, encloses abundant perisperm. The endosperm and embryo are situated laterally at the terminal part of the seed. The perisperm is distinguished into an outer protein-rich zone and inner starch-filled zone. Starch and protein are also deposited in the mature pericarpic tissue. Lipid bodies are seem in the form of oil globules in oil cells.

• 한국잡초학회지
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• 제31권1호
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• pp.96-102
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• 2011

식물 정유로부터 신규 제초제 개발을 위한 살초활성물질을 탐색하였다. 실험에 사용된 10종의 식물정유는[basil(Ocimum basilicum), blackpepper(Piper nigrum), clary sage(Salvia sclarea), ginger(Zingiber pfficinale), hyssop(Hyssopus officinalis), nutmag (Myristica fragrance), palmarosa(Cymbopogon martini), fennel(Foeniculum vulgare), sage(Salvia leucantha), spearmint(Mentha spicta)] 등이었으며, 이중 palmarosa 정유가 유채에 대한 살초력이 가장 높았다($GR_{50}$값, $201{\mu}g\;mL^{-1}$). Palmarosa 정유에 함유된 휘발성 유기화합물로는 alpha-humulene, betacaryophyllen, cis-ocimene, citral, dipentene, geraniol, geranyl acetate, myrcene, nerol, terpinolene, transocimene이었다. Palmarosa 정유에는 geraniol(40.23%), geraniol acetate(15.57%), cis-ocimene(10.79%), beta-caryophyllene(8.72%)이 다량 함유되어 있었고, geraniol과 citral의 $GR_{50}$값은 각각 151과 $224{\mu}g\;mL^{-1}$로 매우 낮았으며 이들 2종의 휘발성 유기화합물이 palmarosa 정유의 살초력에 크게 기여했다고 추론된다. 온실조건에서 palmarosa 정유 $80kg\;ha^{-1}$ 경엽처리는 수수, 돌피, 개밀, 바랭이, 자귀풀, 어저귀, 도꼬마리, 메꽃을 완전 방제하였다. 포장조건에서 민들레, 망초, 쇠비름은 $40kg\;ha^{-1}$ 경엽처리에도 모두 100% 방제가 이루어졌으나 질경이의 방제가는 50% 이었다. 본 연구 결과는 palmarosa 정유에 함유되어 있는 geraniol과 citral이 살초활성에 크게 기여했다는 것을 시사하여 준다.

• 생약학회지
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• 제8권3호
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• pp.115-119
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• 1977

Black pepper (Piper nigrum L.) among several irritating spices tested was highly effective on the duration of hexobarbital hypnosis in mice. Pretreatment of mice with the methanolic extract of black pepper (60mg/kg i.p.) prolonged markedly the duration of hexobarbital sleeping time. Three consecutive daily administrations of the same dose of black pepper extract, however, shortened (37%) the duration of hexobarbital sleeping time. The ether soluble fraction of black pepper extract caused most potent effects on the duration of hexobarbital hypnosis. From the above results, it was postulated that the lipid soluble components of black pepper might considerably change the drug action and metabolism by altering drug metabolizing enzyme systems.

• 생약학회지
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• 제34권1호통권132호
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• pp.86-90
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• 2003

For the determination of inhibiting cytochrome P450(CYP)3A4 activity, an improvement HPLC method was established by using a new internal standard and solvent system. Moreover, CYP3A4 amount for a optimum reaction of enzyme was determined by a comparative study with a variety concentration of enzyme. Using a established method, inhibitory effect of CYP3A4 that is drug metabolizing enzyme Investigated on EtOAc extracts of 5-class spices. As a result of experiment, EtOAc extract of white pepper (Piper nigrum L.) showed strong inhibitory activity. On a continuous experiment, the fraction 2, 4 and 5 of while pepper extract showed remarkable inhibitory activity. Pipeline, a main constituent of pepper was not included in these fraction. It is suggested that major compounds for the inhibitory activity of white pepper may be other ingredient that is not piperine.