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http://dx.doi.org/10.20307/nps.2019.25.3.255

Anti-oxidative Effect of Piperine from Piper nigrum L. in Caenorhabditis elegans  

Park, Hyun Mee (College of Pharmacy, Woosuk University)
Kim, Jun Hyeong (College of Pharmacy, Woosuk University)
Kim, Dae Keun (College of Pharmacy, Woosuk University)
Publication Information
Natural Product Sciences / v.25, no.3, 2019 , pp. 255-260 More about this Journal
Abstract
Piper nigrum L. (Piperaceae), which is a well-known food seasoning, has been used as a traditional medicine for the treatment of vomiting, abdominal pain, diarrhea and anorexia in Korea, China and Japan. Methanol extract from the fruit of P. nigrum was successively partitioned as n-hexane, methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions. Among those fractions the ethyl acetate soluble fraction showed the most potent DPPH radical scavenging activity, and piperine was isolated from the ethyl acetate fraction. To know the antioxidant activity of piperine, we tested the activities of superoxide dismutase (SOD) and catalase together with oxidative stress tolerance and intracellular ROS level in Caenorhabditis elegans. To investigate whether piperine-mediated increased stress tolerance was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain including CF1553. Consequently, piperine enhanced SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, piperine-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.
Keywords
Piperine; Piper nigrum L.; Caenorhabditis elegans; Antioxidant activity;
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1 Nikolic, M. M.; Jovanovic, K. K.; Markovic, T. L.; Markovic, D. L.; Gligorijevic, N. N.; Radulovic, S. S.; Kostic, M.; Glamoclija, J. M.; Sokovic, M. D. J. Pharm. Pharmacol. 2017, 69, 1606-1614.   DOI
2 Ternes, W.; Krause, E. L. Anal. Bioanal. Chem. 2002, 374, 155-160.   DOI
3 Brenner, S. Genetics 1974, 77, 71-94.   DOI
4 Mekheimer, R. A.; Sayed, A. A.; Ahmed, E. A. J. Med. Chem. 2012, 55, 4169-4177.   DOI
5 Aebi, H. Methods Enzymol. 1984, 105, 121-126.   DOI
6 Kim, H. N.; Seo, H. W.; Kim, B. S.; Lim, H. J.; Lee, H, N.; Park, J. S.; Yoon, Y. J.; Oh, J. W.; Oh, M. J.; Kwon, J.; Oh, C. H.; Cha, D. S.; Jeon, H. Nat. Prod. Sci. 2015, 21, 128-133.
7 Seo, H. W.; Cheon, S. M.; Lee, M. H.; Kim, H. J.; Jeon, H.; Cha, D. S. Evid. Based Complement. Alternat. Med. 2015, 2015, 524878.
8 Waters, J. C. J. Cell Biol. 2009, 185, 1135-1148.   DOI
9 Kudryavtseva, A. V.; Krasnov, G. S.; Dmitriev, A. A; Alekseev, B. Y.; Kardymon, O. L.; Sadritdinova, A. F.; Fedorova, M. S.; Pokrovsky, A. V.; Melnikova, N. V.; Kaprin, A. D.; Moskalev, A. A.; Snezhkina, A. V. Oncotarget 2016, 7, 44879-44905.   DOI
10 Surco-Laos, F.; Duenas, M.; Gonzalez-Manzano, S.; Cabello, J.; Santos-Buelga, C.; Gonzalez-Paramas, A. M. Food Res. Int. 2012, 46, 514-521.   DOI
11 Liao, C. Y.; Kennedy, B. K. Cell Res. 2016, 26, 143-144.   DOI
12 Mittal, R.; Gupta, R. L. Methods Find. Exp. Clin. Pharmacol. 2000, 22, 271-274.   DOI
13 Maurya, P. K.; Noto, C.; Rizzo, L. B.; Rios, A. C.; Nunes, S. O.; Barbosa, D. S.; Sethi, S.; Zeni, M.; Mansur, R. B.; Maes, M.; Brietzke, E. Prog. Neuropsychopharmacol. Biol. Psychiatry 2016, 65, 134-144.   DOI
14 Heink, A. E.; Parrish, A. N.; Thorgaard, G. H.; Carter, P. A. Aquat. Toxicol. 2013, 144-145, 75-82.   DOI
15 Homayouni-Tabrizi, M.; Asoodeh, A.; Soltani, M. J. Food Drug Anal. 2017, 25, 567-575.   DOI
16 Verma, N.; Bal, S.; Gupta, R.; Aggarwal, N.; Yadav, A. J. Diet. Suppl. 2018, 9, 1-12.   DOI
17 Pizzino, G.; Irrera, N.; Cucinotta, M.; Pallio, G.; Mannino, F.; Arcoraci, V.; Squadrito, F.; Altavilla, D.; Bitto, A. Oxid. Med. Cell Longev. 2017, 2017, 8416763.
18 Umar, S.; Golam Sarwar, A. H.; Umar, K.; Ahmad, N.; Sajad, M.; Ahmad, S.; Katiyar, C. K.; Khan, H. A. Cell Immunol. 2013, 284, 51-59.   DOI
19 Parveen, A.; Akash, M. S.; Rehman, K.; Kyunn, W. W. Crit. Rev. Eukaryot. Gene Expr. 2016, 26, 143-160.   DOI
20 Prasad, S.; Gupta, S. C.; Tyagi, A. K. Cancer Lett. 2017, 387, 95-105.   DOI
21 Partridge, L; Gems, D. Nat. Rev. Genet. 2002, 3, 165-175.   DOI
22 Weinert, B. T.; Timiras, P. S. J. Appl. Physiol. 2003, 95, 1706-1716.   DOI
23 Deng, Y.; Sriwiriyajan, S.; Tedasen, A.; Hiransai, P.; Graidist, P. J. Ethnopharmacol. 2016, 188, 87-95.   DOI
24 Tasleem, F.; Azhar, I.; Ali, S. N.; Perveen, S.; Mahmood, Z. A. Asian Pac. J. Trop. Med. 2014, 7S1, S461-S468.   DOI