• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.93-102
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• 2019

The materials of artificial soils in the production process of container seedlings have a great influence on plant growth. Peat moss, vermiculite, and perlite have been used as major components of artificial soils for many years; however, they could decrease carbon fixation carried out by the soil and cause environmental problems such as a change in the water quality. Thus, environmental friendly materials to replace them must be developed. The purpose of this study was to verify the optimum additive materials of artificial soils and their mixed ratios for the growth and seedling quality index (SQI) of Quercus serrata. Rice husk, mushroom sawdust, and pine bark were each used as an additive material and mixed into the growth medium at 10% and 20% of the total volume. There was no significant difference in the height growth of Q. serrata. The 20% mushroom sawdust decreased the root collar diameter by 23.4% compared to the control. The total dry weight was highest with the 10% rice husk and was significantly lower by 10.3% for the 20% mushroom sawdust compared to the control. Additionally, the SQI for all the treatments showed no tendency to increase compared with the control. Thus, this study showed the possibility of recycling biomaterials from agriculture and forest for seedling production. This method could reduce environmental problems and help eco-friendly nurseries to achieve a carbon negative impact by the recycling of by-products.

• Journal of Mushroom
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• v.21 no.3
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• pp.190-193
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• 2023

To cultivate pine mushroom (Tricholoma matsutake) artificially, co-cultivation with microorganisms has been introduced. Here, experiments were performed to assess the growth-promoting effect of bacteria on T. matsutake mycelia. Bacteria were isolated from soil samples collected in Yangyang County, Korea. Four of the bacterial isolates (Y22_B06, Y22_B11, Y22_B18, and Y22_B22) exhibited a growth-promoting effect on T. matsutake mycelia (154.67%, 125.91%, 134.06%, and 158.28%, respectively). To analyze the characteristics of the bacteria, especially the antifungal activity, 𝛼-amylase and cellulase activity assays were performed. In comparison with the controls, the isolated bacteria exhibited low 𝛼-amylase and cellulase activity. 16S rRNA gene sequencing was performed to identify the four bacterial isolates. The isolates belonged to the Terrabacteria group and were identified as Microbacterium paraoxydans, Paenibacillus castaneae, Peribacillus frigoritolerans, and P. butanolivorans. These bacterial isolates are expected to have contributed to the growth promotion of T. matsutake mycelia and the artificial cultivation of T. matsutake.

• Journal of Korean Society of Forest Science
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• v.87 no.4
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• pp.519-527
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• 1998

An economic study of vegetation control to increase production of Songyi (Korean name far pine mushroom, matsutake, Tracholoma matsutake (S. Ito & Imai) Sing.) in red pine (Panes densiflora) stands in Korea was undertaken. In Korea, Songyi grows only in red pine stands. Harvest of this mushroom provides a significant income source to rural people in Korea yielding exports of US$20 million to US$80 million per year. However, hypogeous Songyi colonies and the mushroom production are declining, partly because shade tolerant species are succeeding the shade intolerant red pine. Past research says that it is possible to deep Songyi production increasing by controlling under-story vegetation. But few people are wilting to invest in the necessary control. Our analysis found that the economics of vegetation control appear to be quite favorable, showing an internal rate of return (IRR) of 20.7 percent in 15 years. However, positive returns do not occur for at least eight years and even then, the returns may not appear to the landowner to be a result of vegetation control efforts only because the mushroom production has been greatly variable depending on weather conditions. In a sensitivity analysis, it was found that the number of circular mushroom colonies was critically important for the cash flow. Results of this analysis are also sensitive to assumptions about annual growth length(0.16m radial growth=1.0m/circular length growth) of Songyi colony. However, the primary goal of vegetation control should be to keep the young colonies growing. Further research in the behavior of hypogeous Songyi colonies after vegetation control would help to improve our confidence in the results.

• Journal of Mushroom
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• v.3 no.3
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• pp.105-108
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• 2005

Main materials used as media for oyster mushroom cultivation are pine sawdust, beet pulp, cotton seed pomace. Increases in the price and the unbalance of demand and supply of cotton seed pomace was often damage to oyster mushroom cultivation farm, so we investigated agricultural by-product to replace the cotton seed pomace for bottle cultivation of oyster mushroom. Treatment of coconut oil meal or coconut pomace delayed incubation period about 3 days compared with cotton seed waste treatment(control), but yield and income index showed similar to each other in three treatment. Consequently coconut oil meal and coconut pomace could select for cotton seed pomace substitute.

• The Korean Journal of Mycology
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• v.49 no.4
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• pp.469-476
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• 2021

Tricholoma matsutake is commercially important wild edible mushroom. The only method for cultivation of T. matsutake has been to use matsutake-infected pine trees. In this study, twenty-one fruiting bodies were collected from the matsutake-infected pine trees in the Hongcheon experimental site in 2020. The fruiting bodies from the existing production area of matsutake-infected pine tree and from its transplantation site were found to be identical through monitoring and analysis of 13 microsatellite markers. Transplanted matsutake-infected pine trees succeeded in fairy-ring formation and matsutake fruiting in 2010 and 2017 to 2020. In conclusion, the matsutake mycelium of transplanted matsutake-infected pine tree originates from the existing matsutake-infected pine tree production.

• Journal of Forest and Environmental Science
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• v.35 no.1
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• pp.25-30
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• 2019

The composition of artificial soil in a containerized seedling production plays an important role in seedling quality as well as environmental issues. We investigated the effects of different types of biomaterials and mixed ratio with artificial soil on the growth of Fraxinus rhynchophylla seedlings. Soil medium was supplemented with 3 levels (0%, 10%, 20%) of pine bark, mushroom sawdust and rice husk. Root collar diameter (RCD), height growth, and biomass have significantly increased when rice husk was applied. Compared with the control, RCD and height growth showed highest in 20% rice husk treatment with an increase of 5.7% and 17.6%, respectively. In contrast, the treatments of pine bark and mushroom sawdust showed lower results in growth parameters (RCD, height growth, and total biomass) than control. Seedling quality index was also highest at the 20% rice husk treatment, but there was not statistically different among treatments. Our results suggested rice husk can be substituted up to 20% of substrates for containerized F. rhynchophylla seedling production system.

• Current Research on Agriculture and Life Sciences
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• v.3
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• pp.105-111
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• 1985

This research is mainly focused on the distributions of pine mushroom(Tricholomo matsutake S.) in Kyungpook province, south eastern part of Korea. The study contributes to graps the trends of producing place and their distributions. The general trends of pine mushroom producing area are scattered over northern part of the province : such as Ulchin Kun, Youngyang Kun, and Bonghwa Kun. The term 'Kun' is equivalent to a country level district of administration. Less concentrated areas are listed : Munkyong Kun, Sangju Kun, Yecheon Kun, Euiseong Kun, Dalseong Kun, Kyungsan Kun, and Cheongdo Kun, which have shown growing spots in pine forest area Pine mushroom do not grow in south eastern part of province, which are Kumreung Kun, Sangju Kun, Chilgog Kun, Wolsong Kun, Koryong Kun. Producing spots classified by Kun area : 117 spots in Ulchin Kun, 70spots in Yongdeog Kun, 54 spots in Youngyang Kun, 38 spots in Munkyong Kun, 37 spots in Cheongsong Kun, 32 spots in Andong Kun, 32 spots in Sangju Kun, 26 spots in Bonghwa, 17 spots in Youngil Kun 15 spots in Yeongcheon Kun, 15 spots in Euiseong Kun, 10 spots in Gyeongsan Kun, 9 spots in Gunwi Kun, 6 spots in Cheongdo Kun, 5 spots in Yecheon Kun, 4 spots in Seonsan Kun, 2 spots in Chilgog Kun, and one spot in Weolseoog Kun.

• The Korean Journal of Mycology
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• v.3 no.2
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• pp.19-23
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• 1975

A study on the variation of the air temperature in the pine mushroom box containing dry ice for the preservation of the freshness of the pine mushroom, Tricholoma matsutake (Ito et Imai) Sing. produced in Korea was made in Seoul, Korea during the period of August 1 to 30, 1975. A safe shipment of 5 kg (net weight) of fresh mushrooms with freshness preserved for 24 hours in August required the placement of 4.5 kg of dry ice-a half in the upper portion and the other half in the middle portion-in an airtight container (mushroom box). The container used for the shipment test, made of 3mm-thick double-layer cardboard, was of the dimensions : $0.33{\times}0.4{\times}0.5=0.066(m^3)$. As the temperature fell, decreased amounts of dry ice were needed : 3 to 2 kg in September and 1.5 to 0.3 kg in October. With this preservation method, large quantities of fresh mushrooms were exported to Tokyo ana Osaka, Japan without damage in the process of transportation.

• The Korean Journal of Mycology
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• v.8 no.1
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• pp.7-11
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• 1980

To increase production of Tricholoma matsutake, experiments were carried out in a mountain located in Keumma 2-ri, Joocheon-myun, Yongwol-gun, Kangwon-do, Korea during the period from April to November 1979. The area of the experiment showed pH 6.55 of soil acidity and the rock was found to be granite. Among its vegetation, the major growths were Pinus densiflora Siebold et Zuccarini, Rhododendron mucronulatum Turcz., Rhododendron schlippenhachii Max., Lespedeza bicolor and Pteridium aquilinum (Linne) Kuhn var. japonicum Nakai. Of two equally divided areas of A and B in which pine mushrooms grew, artificial methods such as thinning the upper portion of land raking and water-spraying were applied to area B only, where 21% increase of the production was obtained as compared with the control area A.

• Korean Journal of Agricultural Science
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• v.48 no.3
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• pp.493-503
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• 2021

Tricholoma matsutake (Pinus mushroom, PM) is one of the most valued ectomycorrhizal fungi in Asia because it is an expensive forest product with a unique flavor and taste. Therefore, many studies have tried to successfully cultivate Tricholoma matsutake artificially in Korea and other countries. However, its physiological and ecological characteristics are still unknown. Thus, we need to understand the diversity and clusters of microorganisms related to Tricholoma matsutake and to identify their core microorganisms related to their growth and production. In this study, we obtained an average of 11,661 fragments from three pine mushrooms with metagenome (an assemblage of genes of all microorganisms in the natural world) analysis from a pine forest located in Pohang, Gyeongsang-Bukdo. Of these, the valid reads were on average 5,073 per sample available for analysis, and the average length of a read was 456 bp. There were an average of 33.3 phyla in the metagenome analysis. Firmicutes phylum made up on an average 46% of the phyla and was dominant among the phyla. The next dominant phylum was Proteobacteria at 27% followed by Bacteroidetes at 17%, Actinobacteria at 5% and Verrucomicrobia at 2%. The Proteobacteria phylum consisted of the γ-proteobacteria class at 54% followed by β-proteobacteria at 37%, α-proteobacteria at 6%, δ-proteobacteria at 2% and ζ-proteobacteria at 0%. The metagenome consisted of the Ruminococcaceae family at 17% followed by Pseudomonadaceae at 13%, Burkholderiaceae at 7%, Bacteroidaceae at 7%, Lachnospiraceae at 7% and Clostridiaceae at 6%.