Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Growth-promoting effect on Tricholoma matsutake mycelium by Terrabacteria isolated from pine mushroom habitats in Korea

국내 송이 자생지에서 분리된 Terrabacteria에 의한 송이균사체 생장촉진 효과

  • Doo-Ho Choi (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jae-Gu Han (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kang-Hyo Lee (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Gi-Hong An (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA)
  • 최두호 (농진청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 한재구 (농진청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 이강효 (농진청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 안기홍 (농진청 국립원예특작과학원 인삼특작부 버섯과)
  • Received : 2023.08.24
  • Accepted : 2023.09.17
  • Published : 2023.09.30
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Abstract

To cultivate pine mushroom (Tricholoma matsutake) artificially, co-cultivation with microorganisms has been introduced. Here, experiments were performed to assess the growth-promoting effect of bacteria on T. matsutake mycelia. Bacteria were isolated from soil samples collected in Yangyang County, Korea. Four of the bacterial isolates (Y22_B06, Y22_B11, Y22_B18, and Y22_B22) exhibited a growth-promoting effect on T. matsutake mycelia (154.67%, 125.91%, 134.06%, and 158.28%, respectively). To analyze the characteristics of the bacteria, especially the antifungal activity, 𝛼-amylase and cellulase activity assays were performed. In comparison with the controls, the isolated bacteria exhibited low 𝛼-amylase and cellulase activity. 16S rRNA gene sequencing was performed to identify the four bacterial isolates. The isolates belonged to the Terrabacteria group and were identified as Microbacterium paraoxydans, Paenibacillus castaneae, Peribacillus frigoritolerans, and P. butanolivorans. These bacterial isolates are expected to have contributed to the growth promotion of T. matsutake mycelia and the artificial cultivation of T. matsutake.

송이버섯을 인공재배하기 위하여 송이 근권토양의 미생물을 활용하여 본 실험을 진행하였다. 본 실험은 송이의 자실체가 나오기 전인 7월의 토양으로부터 세균을 확보하여 진행되었으며 총 4점의 세균에 대해 송이 균사체 생장촉진을 확인하였다. 확보된 세균들, Y22_B06, Y22_B11, Y22_B18, Y22_B22는 각각 송이 균사의 면적을 각각 154.67%, 125.91%, 134.06%, 158.28%로 생장시키는 것으로 확인되었다. 또한 해당 균들에 대한 동정도 이뤄졌으며 각각 Mic. paraoxydans, Pae. castaneae, Per. frigoritolerans, Per. butanolivorans 로 확인되었으며, 이중 Paenibacillus 속을 제외하고는 송이 균사체 생장촉진 사례가 보고되지 않았다. 본 실험의 결과를 동일한 실험조건인 기존 사례(Oh et al., 2018a)와 비교하였으며, Pae. latus에 의한 230%의 송이 균사 생장촉진의 사례 등과 비교하면 그 생장촉진능은 떨어진다고 볼 수 있다. 또한 세균 자체에 의한 항진균성이 낮아 송이 근권토양에서 진균과의 경쟁에서 우위에 있다고 보기 힘들다. 그러나 송이 균사체에 대해 유의미한 생장촉진을 이끌어냈기에 본 실험의 세균 4점은 송이 생장 촉진에 대한 유효성이 있다고 판단되며 해당 세균을 이용한 배양액을 직접적으로 송이 균사체 혹은 송이 자실체에 적용하였을 경우 송이 생장에 긍정적인 영향을 끼칠 것으로 기대되며 이에 대한 추가적인 실험이 진행되어야 할 필요가 있다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 국립원예특작과학원 시험연구사업(과제번호 PJ014766022023)에 의하여 수행된 결과의 일부로써 국립원예특작과학원 전문연구원 과정 지원사업에 의해 이루어진 것이며, 이에 감사드립니다.

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