• Food Science and Preservation
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• v.14 no.2
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• pp.154-159
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• 2007

In efforts to use pine needle extract as a substitute for sugar, fermented pine needle extract syrup was added to the wheat flour nea in the manufacture of white bread The extract was added to levels of 8.3%, 11% and 18% of total weight based on the Brix degree of the dough The cohesion of each dough was checked, and the physical properties and storage stabilities of the baked breads were analyzed during storage at mom temperature. Analysis of the cohesion of each dough by farinogram showed that dough with pine needle extract was better than dough with sugar, in terms of both stability and durability of mixing. The pH of dough with syrup was steady during storage at pH 5.4 5.8, which is favorable for yeast activity. The dough with syrup also showed low firmness and good extensibility, both of which would favorably affect gas retention on fermentation. Increases in syrup addition resulted in higher product volumes. Bread with syrup was slow to increase in hardness during storage, suggesting that higher syrup concentrations inhibited development of staleness. The addition of syrup also inhibited the growth of aerobic bacteria and mold on the bread surface. The addition of syn did not cause any negative effect. The use of pine none extract syrup may thus contribute to improvements the physical properties, the storage stability, and the hygienic quality of bread.

• The Korean Journal of Community Living Science
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• v.19 no.2
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• pp.213-221
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• 2008

In this study, a dye bath was made using a mixture of distilled water, pine needle extract and bamboo vinegar. Analysis and comparison of various functions of cotton fabrics dyed using bamboo charcoal and gardenia seeds in distilled water and in the dye bath mixture were done. The summary of the results is as follows. Comparing bamboo vinegar and the pine needle extracts, the bamboo vinegar had a higher antibacterial function. In the case of natural dyeing cotton fabrics using bamboo charcoal and gardenia seeds, the additional amount of dyestuff for optimization is 10 g and 20 g respectively per 1 liter of distilled water. The suitable dye bath mixture ratio of distilled water, pine needle extract, and bamboo vinegar considering functionality and economical efficiency of dyed fabrics is 4:3:3. By using the mixture made in this study as a dye bath, improved antibacterial function, deodorization, and colorfastness can be obtained more than distilled water alone.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.3
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• pp.461-470
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• 1998

The effects of addition of water extract of pine needle(WEPN) on texture and cell wall polysac-charides content of kimchi during fermentation at 1$0^{\circ}C$ were investigated. Textural properties of hardness, gumminess and cohesiveness of kimchi were higher for WEPN-added kimchi than for the control during the entire fermentation periods, while its adhesiveness was lower. Alcohol insoluble substance, among cell wall polysaccharide fractions of kimchi was higher in WEPN-added kimchi than in the control but water soluble materals was high in control during fermentation periods. The separation phenomenon of middle lamella of control kimchi tissue was observed at 14th days of fermentation but WEPN-added kimchi showed at 21th days fermentation. The vasular of kimchi tissue was more destroyed in control than in WEPN-added kimchi.

• Journal of Korean Society on Water Environment
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• v.33 no.1
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• pp.8-14
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• 2017

Microcystis aeruginosa (M. aeruginosa) is a cyanobacterium species that can form harmful algal blooms in freshwater bodies worldwide. The use of pine needle extract (PNE) to control nuisance algae by allelopathic inhibition will be environmentally friendly and promising. PNE removed successfully upto 98% of M. aeruginosa at the following optimal conditions: pH 7, $25^{\circ}C$ of temperature, 100 rpm of mixing rate, 5 min of mixing time. These results was indicated that the amount of 1 g/L PNE was removed 1g dryweight/L of M. aeruginosa. The kinetic data showed substrate inhibition kinetics and maximum growth rate was obtained when the M. aeruginosa was grown in medium containing 0.5 g/L of initial concentration of PNE. Different substrate inhibition models were fitted to the kinetic data and found the Luong model was best. The model predicted kinetic parameters were in agreement with the experimental findings. The natural extract, PNE, can be a promising inhibition due to its high efficiency and low dose requirements.

• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.215-219
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• 2012

The strain KCTC 11629BP, isolated from spontaneously fermented pine needle extract (FPE), showed fibrinolysis activity. The isolated strain was analyzed in physiological and biochemical experiments. Based on 16S rDNA sequencing and phylogenic tree analysis, the strain was identified to be a part of the genus Acetobacter, with Acetobacter senegalensis and Acetobacter tropicalis as the closest phylogenetic neighbors. Based on genotypic and phenotypic results, it was proposed that bacterial strain KCTC 11629BP represents a species of the genus Acetobacter. The strain was thusly named Acetobacter sp. FP1. In conclusion, Acetobacter sp. FP1 isolated from FPE possesses fibrinolytic activity.

• Journal of Korean Society of Forest Science
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• v.62 no.1
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• pp.87-95
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• 1983

The effects of the pine needle gall midge, Thecodiplosis japonensis, on the growth of the Korean red pine, Pinus densiflora, were examined for the natural forest stands at nine locations: Seocheon-gun, Boryong-gun, and Hongseong-gun in Chooncheongnam-do, where consequence of the infestation had been different. To estimate the economic threshold level of the pine needle gall midge, the growth impacts due to the insect were examined with terminal shoot growth, and cambial growth by analyzing the growth horizontally and vertically. The results obtained are as follows: 1) In general, the growth impacts of the red pine trees were significant for the five consecutive years since damage had occurred, and its maximum retardation was shown in the second and the third years. 2) Simultaneous retardation occurred in both terminal and cambial growth of the tree, probably due to cumulative characteristics of the feeding behaviour. 3) Strong correlation was found between terminal and cambial growth of Korean red pine and this was true for the trees infested by pine needle gall midge. Thus retardation in cambial growth due to the insect may be estimated with that in terminal growth. 4) Infestation was greater on slope facing north than south even though the history of the infestation had been similar. 5) The stem volume of 13-year-old, infested Korean red pine was reduced by more than 75-85%, compared to same aged, uninfected pine in yield table. Thus, much longer period is required to recover the normal growth.

• The Korean Journal of Ecology
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• v.15 no.2
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• pp.147-155
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• 1992

Litterfall, decomposition, and dynamics of n and p in decomposing litter were investigated for 2 years in red pine ( pinus densiflora) and chinese thuja( thuja orientalis)stands in the limestone area. Average litterfall in red pine and chinese thuja stands were 4, 535kgDM ha-1 yr-1 and 5, 010 kgDM ha-1 yr-1, respectively. Seasonal litterfall in red pine and chinese thuja stands showed peaks in November. concentrations of N and P in the needle litter were lowest in the winter when the greast litterfall occurred, and highest in the summer when the least litterfall occurred. However, those in chinese thuja scale leaf litter showed litter seasonal variation.amount of N and P returned to the forest floor through litterfall were 29.02kgN ha-1 yr-1 , 2.81 kg P ha-1 yr-1 for red pine stand, and 31.06 kg N ha-1 yr-1 , 2.86kgP ha-1 yr-1 for chinese thuja stand, respectively. After 21 mounts elapsed, needle and chinese thuja scale leaf litterbags lost 34.8% and 32.5% of the initial weight, respectively. N concentrations in the docomposing needle and chines thuja scale leaf litter decreased by 19% and 30%, respectively, after 1 month elapsed, and then gradually increased to exceed the initial concentration after 9 months elapsed and then gradually increased to exceed the initial concentration after 9 months elapsed in both of them, P in needle and chines thuja scale leaf litter decreased by 54% and 57% of the initial concentration, respectively, after 1 month elapsed. unlike N, P concentration in the decomposing litter did not exceed that of initial ones. Neyt immobilization period of N and P in decomposing litter did not occur over the study period.

• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.213-217
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• 1998

This study was performed to observe cytotoxic effect of the pine needle extracts against cancer cell lines including human gastric carcinoma (KATOIII), human lung carcinoma (A549), human hepatocellular carcinoma (Hep3B) and human breast adenocarcinoma (MCF-7) using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) and SRB (sulforhodamine B) method. The extracts were prepared by step-wise fractionation of ethanol extract of pine needles using diethylether, chloroform, ethylacetate, butanol and water. The growth of the cancer cells in medium containing pine needle extracts were significantly inhibited degree in proportion to the increase of the extract concentration. A significant shrinkage of Hep3B cells was observed when the cells were exposed into 0.5, 1 mg/mL of pinus rigida extract.

• KSBB Journal
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• v.26 no.2
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• pp.172-176
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• 2011

This study was aimed to verify suppressive effect of pine-needle extract on lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression. In order to evaluate suppressive effect on iNOS expression, RAW 264.7 cells were stably transfected using an iNOS promoterluciferase reporter plasmid yielding RAW 264.7/pGL2-NeomiNOS_ pro11 cells. Established in vitro detection system revealed to diminish LPS-induced iNOS expression by 0.1~500 ${\mu}g/mL$ of saponin at the concentration-dependant manner. Pine needle extract also diminished LPS-induced iNOS expression to 92 and 88% at 500 and 50 ${\mu}g/mL$, respectively. These results suggest that the in vitro detection system developed here could be useful for the verification of suppressive materials on iNOS expression and pine needle extract could be used for the development of functional foods.

• Natural Product Sciences
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• v.19 no.4
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• pp.290-296
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• 2013

In preliminary tests, we examined the effect of several fractions isolated from fermented pine needle extract on pacemaker potentials in cultured interstitial cells of Cajal (ICCs) from the mouse colon using a whole cell patch clamp technique. Among these fractions, Fraction 3 (F3) elicited the most powerful depolarization of membrane. Therefore, the aim of the present study was to investigate the effect of F3 obtained from fermented extract of Pinus densiflora needle on pacemaker potentials in ICCs and to establish its mechanism of action. Colonic ICCs generated spontaneous periodic pacemaker potentials in the current-clamp mode. F3 depolarized the membrane and decreased the frequency and amplitude of pacemaker potentials in a dose-dependent fashion. The F3-induced effects on pacemaker potentials were blocked by methoctramine, a muscarinic $M_2$ receptor antagonist, and by glycopyrrolate, a muscarinic $M_3$ receptor antagonist. The F3-induced effects on pacemaker potentials were blocked by external $Na^+$-free solution and by flufenamic acid, a non-selective cation channel blocker, as well as by the removal of external $Ca^{2+}$ and in the presence of thapsigargin, a $Ca^{2+}$-ATPase inhibitor in the endoplasmic reticulum. Taken together, these results suggest that F3 of pine needle extract modulates the pacemaker activity of colonic ICCs by the activation of non-selective cation channels via muscarinic $M_2$ and $M_3$ receptors. And external $Ca^{2+}$ influx and intracellular $Ca^{2+}$ release are involved in F3 actions on ICCs.