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Effects of Pine Needle Extract on Spontaneous Pacemaker Potentials in Interstitial Cells of Cajal from the Mouse Colon  

Shahi, Pawan Kumar (Department of Physiology, College of Medicine, Chosun University)
Zuo, Dong Chaun (Department of Physiology, College of Medicine, Chosun University)
Choi, Seok (Department of Physiology, College of Medicine, Chosun University)
Lee, Mi Jung (Department of Physiology, College of Medicine, Chosun University)
Cheong, Hyeon Sook (Department of Life Science, College of Natural Science, Chosun University)
Lim, Dong Yoon (Department of Pharmacology, College of Medicine, Chosun University)
Jun, Jae Yeoul (Department of Physiology, College of Medicine, Chosun University)
Publication Information
Natural Product Sciences / v.19, no.4, 2013 , pp. 290-296 More about this Journal
Abstract
In preliminary tests, we examined the effect of several fractions isolated from fermented pine needle extract on pacemaker potentials in cultured interstitial cells of Cajal (ICCs) from the mouse colon using a whole cell patch clamp technique. Among these fractions, Fraction 3 (F3) elicited the most powerful depolarization of membrane. Therefore, the aim of the present study was to investigate the effect of F3 obtained from fermented extract of Pinus densiflora needle on pacemaker potentials in ICCs and to establish its mechanism of action. Colonic ICCs generated spontaneous periodic pacemaker potentials in the current-clamp mode. F3 depolarized the membrane and decreased the frequency and amplitude of pacemaker potentials in a dose-dependent fashion. The F3-induced effects on pacemaker potentials were blocked by methoctramine, a muscarinic $M_2$ receptor antagonist, and by glycopyrrolate, a muscarinic $M_3$ receptor antagonist. The F3-induced effects on pacemaker potentials were blocked by external $Na^+$-free solution and by flufenamic acid, a non-selective cation channel blocker, as well as by the removal of external $Ca^{2+}$ and in the presence of thapsigargin, a $Ca^{2+}$-ATPase inhibitor in the endoplasmic reticulum. Taken together, these results suggest that F3 of pine needle extract modulates the pacemaker activity of colonic ICCs by the activation of non-selective cation channels via muscarinic $M_2$ and $M_3$ receptors. And external $Ca^{2+}$ influx and intracellular $Ca^{2+}$ release are involved in F3 actions on ICCs.
Keywords
Fraction of pine needle extract; Interstitial cells of Cajal; Colon; Muscarinic receptor;
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