• Korean Journal of Agricultural Science
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• v.48 no.3
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• pp.493-503
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• 2021

Tricholoma matsutake (Pinus mushroom, PM) is one of the most valued ectomycorrhizal fungi in Asia because it is an expensive forest product with a unique flavor and taste. Therefore, many studies have tried to successfully cultivate Tricholoma matsutake artificially in Korea and other countries. However, its physiological and ecological characteristics are still unknown. Thus, we need to understand the diversity and clusters of microorganisms related to Tricholoma matsutake and to identify their core microorganisms related to their growth and production. In this study, we obtained an average of 11,661 fragments from three pine mushrooms with metagenome (an assemblage of genes of all microorganisms in the natural world) analysis from a pine forest located in Pohang, Gyeongsang-Bukdo. Of these, the valid reads were on average 5,073 per sample available for analysis, and the average length of a read was 456 bp. There were an average of 33.3 phyla in the metagenome analysis. Firmicutes phylum made up on an average 46% of the phyla and was dominant among the phyla. The next dominant phylum was Proteobacteria at 27% followed by Bacteroidetes at 17%, Actinobacteria at 5% and Verrucomicrobia at 2%. The Proteobacteria phylum consisted of the γ-proteobacteria class at 54% followed by β-proteobacteria at 37%, α-proteobacteria at 6%, δ-proteobacteria at 2% and ζ-proteobacteria at 0%. The metagenome consisted of the Ruminococcaceae family at 17% followed by Pseudomonadaceae at 13%, Burkholderiaceae at 7%, Bacteroidaceae at 7%, Lachnospiraceae at 7% and Clostridiaceae at 6%.

• Mycobiology
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• v.38 no.3
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• pp.203-205
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• 2010

The spore of Tricholoma matsutake is considered to be the starting point of the mushroom growth cycle, but the mechanism of mycelial development from the spore stage is not yet clarified. In this study, we tried to measure how far the spores of T. matsutake disperse from a fruiting body located at a Pinus densiflora stand in Korea. We established 16 slide glasses coated with glycerin near a fruiting body in four directions separated by four different distance intervals within a mushroom productive stand after removing all other fruiting bodies from three plots. The number of dispersed spores increased with time from the first day (475 $spores/cm^2$) to the fourth day (836 $spores/cm^2$) after the pileus opened. The number of spores dispersed downward was about 1.5 times greater than that dispersed toward the ride. The number of dispersed spores decreased exponentially as the distance from each fruiting body increased. More than 95% of the spores dropped within a meter from the fruiting body, with 75% dropping within 0.5 m. Even so, the number of spores dispersed over 5 m from the fruiting body was more than 50 million when considering the total number of spores produced by a fruiting body is about 5 billion.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.5
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• pp.535-541
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• 2008

Pine mushroom (Tricholoma matsutake Sing.) is an expensive and highly prized delicacy in Korean and Japanese cuisines with its unique flavor and functional properties. The biological activities of pine mushroom juice (soluble solid contents $4.3^{\circ}$Brix) were evaluated using different tests; DPPH radical scavenging assay for its antioxidant activity, fibrin plate method for fibrinolysis and angiotensin I converting enzyme (ACE) inhibitory activity for anti-hypertensive effect. Free radical scavenging activity of the pine mushroom juice was $48.3{\pm}2.2%$ at the concentration of 1.0 mg/mL. The fibrinolytic activity of pine mushroom was about 2 times greater than that of plasmin used as positive control and the activity increased dose-dependently. The pine mushroom juice inhibited ACE activities dose-dependently and $IC_{50}$ value of ACE activity was $1.03^{\circ}$Brix. These results suggest that pine mushroom is a healthy delicacy.

• Journal of Mushroom
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• v.21 no.3
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• pp.190-193
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• 2023

To cultivate pine mushroom (Tricholoma matsutake) artificially, co-cultivation with microorganisms has been introduced. Here, experiments were performed to assess the growth-promoting effect of bacteria on T. matsutake mycelia. Bacteria were isolated from soil samples collected in Yangyang County, Korea. Four of the bacterial isolates (Y22_B06, Y22_B11, Y22_B18, and Y22_B22) exhibited a growth-promoting effect on T. matsutake mycelia (154.67%, 125.91%, 134.06%, and 158.28%, respectively). To analyze the characteristics of the bacteria, especially the antifungal activity, 𝛼-amylase and cellulase activity assays were performed. In comparison with the controls, the isolated bacteria exhibited low 𝛼-amylase and cellulase activity. 16S rRNA gene sequencing was performed to identify the four bacterial isolates. The isolates belonged to the Terrabacteria group and were identified as Microbacterium paraoxydans, Paenibacillus castaneae, Peribacillus frigoritolerans, and P. butanolivorans. These bacterial isolates are expected to have contributed to the growth promotion of T. matsutake mycelia and the artificial cultivation of T. matsutake.

• The Korean Journal of Mycology
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• v.19 no.3
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• pp.203-213
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• 1991

From 1986 to 1990, the fifty-three sites (shiros) of the five places known as an areas of the pine-mushroom production in Kyoung Sang Do were visited, investigating the biology or ecology of pine-mushrooms. Physical or ecological characteristics of the areas producing pine-mush­rooms was similar to those reported in Japan, but not similar to in this regardings; The ages of pine trees, the understory plant flora of the places producing the pine-mushrooms and the soil types. The areas of pine-mushroom productions are divided to two typical areas, the inland and coastal areas. Ecological differences between two areas were found, The understory plant flora and different soil types. Three kinds of soil were, by the naked eyes, were found from our observa­tions of Kyoung Sang Do, but not based on soil analysis. The pine-mushrooms observed in Kyoung Sang Do were related to the root of the pine trees, but not confirmed to be ectomycorrhizally related to the pine trees. The mycelia isolated form the different places were compared with the degradation of phenolic compounds and culture methods, but were not clearly distinguished. The productions of pine-mushroom and several methods for the pine mushroom were suggested under four years' observations.

• Culinary science and hospitality research
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• v.14 no.4
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• pp.55-66
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• 2008

This study investigated the rheological and sensory characteristics of pickle with frozen pine mushroom. P3(Developed pickle seasoning) was the best by preference among three kinds of pine mushroom pickle seasoning. For flavor and functionality, pine mushroom pickle was processed by using three kinds of method(P3-1: P3+cinnamon 10 g, P3-2: P3+licorice 10 g and P3-3: P3+licorice 5 g+cinnamon 5 g). As a result, the product from P3-3(P3+licorice 5 g+cinnamon 5 g) was the best preferred pine mushroom pickle. The pH value of P3-3 was 2.15, 42.9 degrees Brix, and its color value was L(54.65), a(-1.61), b(17.87). Its texture level was higher than that of other products, but it would be lowered on storage. Until the 28th day of storage, microorganisms in pine mushroom pickle seasoning were detected less than 30 CFU/mL.

• The Korean Journal of Mycology
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• v.26 no.3 s.86
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• pp.306-313
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• 1998

The occurrences of ectomycorrhizal fungi were investigated in a pine-mushroom forest at Hongcheon, Korea. The fairy rings of Tricholoma matsutake were figured by the sporocarp places of T. matsutake for three years $(1995{\sim}1997)$, and the occurrences of other ectomycorrhizal fungi were surveyed with x and y dimensions for two years $(1996{\sim}1997)$. The diameters of fairy rings of T. matsutake ranged from 2m to 10m, which indicated that the age of the fairy rings as $10{\sim}50$ years when we consider that the growth of the fairy ring used to show about 10 cm per year. Russula bella, R. sororia, R. delica and Cantharellus minor were the major species occurred on the site during the survey period, and each species occupied 16.0%, 12.8%, 12.4% and 7.0% of total mushroom occurrence, respectively. From the results, we could conclude that the surveyed stand was a productivity-declining forest from the view point of pine-mushroom production. In addition, Amanita pantherina, Suillus bovinus, Ramaria flaccida and Laccaria amethystea were considered to be the indicator species for declining of pine-mushroom productivity since some fruiting bodies of the species appeared around the fairy ring of T. matsutake.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.95-103
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• 2008

Mushrooms are regarded as one of the well-known foods and biopharmaceutical materials with a great deal of interest. Polysaccharide ${\beta}$-glucan is the major component of mushrooms that displays various biological activities such as antidiabetic, anticancer, and antihyperlipidemic effects. In this study, we compared the immunostimulatory potency of polysaccharide fractions, prepared from liquid culture of pine-mushroom Tricholoma matsutake, with a potent immunogen lipopolysaccharide (LPS), and their molecular mechanisms on the functional activation of macrophages. We found that fraction II (TMF-II) was able to comparably upregulate or highly enhance the phenotypic functions of macrophages such NO production and cytokine (IL-$1{\beta}$, IL-6, IL-12, and TNF-${\alpha}$) expression, to LPS. TMF-II triggered the phosphorylation of $I{\kappa}B{\alpha}$, a critical step for NF-${\kappa}B$ activation and translocation. Of the upstream signaling enzymes tested, Src and Akt were thought to be the responsible upstream signaling components in induction of NO production, although TMF-II strongly upregulated the phosphorylation of all MAPK pathways. Therefore, our data suggest that T. matsutake-derived ${\beta}$-glucan may exert its immunostimulating activities with similar potency to LPS via activation of multiple signaling pathways linked to NF-${\kappa}B$ activation.

• Journal of Mushroom
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• v.2 no.4
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• pp.175-183
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• 2004

We carried out survey of a pine mushroom picking district and its vegetation environment for increase and maintainment of its production in Kyonggi province. The district which we could pick up pine mushroom among the nineteen surveyed district were sixteen such as Geoleun ri Gangcheon myeon Yeoju gun Kyonggi do, etc. and total yields were 470~600 kg in Kyonggi province. The temperature of atmosphere and earth in Yeoju and Bonghwa were droped to $19^{\circ}C$ at 5~7th september and maintained from $12^{\circ}C{\sim}18^{\circ}C$ during the a pine mushroom picking period. The density of a pine tree per $100m^2$ was 5.1~27.1 trees, the years of it was 15~50years old and height was 4~11 meter. The pine tree in a pine mushroom picking district was competed with oak trees, azaleas, lacquers, etc. The depth of organic materials accumulated on the pine mushroom mycelium layer was 4.5 cm in Yeoju. The soil texture of a pine mushroom picking district was almost sandy, soil pH was 4.3~5.1 and organic material content was 3.91~8.28%.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.479-486
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• 2010

We analyzed the relationships between the daily yield and quality of pine-mushroom to predict the annual production pattern and unit price of the mushroom with the records of pine-mushroom trade at Yeongdeok forestry cooperative's market for nine years (2000~2008). Although there were some exceptions due to extreme drought or extraordinary temperature, the production ratio of high quality (first and second grade) was more than 50% in early stage and decreased, while that of low quality (pileus opened and defected ones) showed increasing pattern after the production reached in peak. The ratio of high quality and that of low quality were reversed 1~9 days before the mushroom production reached the acme of daily yield, which allowed us to predict that the mushroom production would be decreased when the ratio of low quality overcomes that of high quality. The ratio of high quality preceded about 3~4 days prior to that of daily yield, and the mushroom yield showed significant correlations with the ratio of high quality mushroom prior to 3~4 days of the day with the coefficient larger than 0.5 (r=0.51 for 3 days and r=0.54 for 4 days). Thus, we concluded that the analysis of grade distribution of pine-mushroom at the market may provide a significant clue to predict production pattern of the mushroom. In addition, the price of high quality pine-mushroom showed clear negative correlations with the yield. Thus, the analysis may take a good role for the trading of pine-mushroom with providing information for predicting the price of pine-mushroom.