• Applied Chemistry for Engineering
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• v.26 no.6
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• pp.648-658
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• 2015

Extraction of phytoncide oil from korea pine cone waste without damaging the pine cone tree itself was investigated using a steam distillation method. Also various components in the extracted phytoncide oil were separated using a column chromatography method. The extraction of phytoncide oil was effectively proceeded, and the maximum production yield of phytoncide oil could be obtained under $100^{\circ}C$ of distillation temperature and within 30 minute of distillation time. According to chemical analysis, it was found that the phytoncide oil from korea pine cone waste was consisted of more than 12 components such as ${\alpha}$-pinene, ${\beta}$-pinene, D-limonene, as main components. In addition, the aqueous hydrogel containing other components such as verbenone, ${\alpha}$-terpinieol, fenchol, different from components of phytoncide oil itself could be obtained through the steam distillation.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2005.11a
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• pp.72-73
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• 2005

This study was carried out to investigate the effects of extracts supplements of pine nut cone on the broiler performance and the cholesterol content, the lipid oxidation of serum and meat of broilers. The control group was fed a common basal diet without antibiotics and the treatment group were fed a common basal diet with extracts of pine nut cone of 500ppm(T1), illite 1% +extracts of pine nut cone of 500ppm(T2), pine nut cone powder of 2.5%(T3) for 5 weeks. The weight gain and the feed intake were significantly higher treatment than control. The cholesterol content of serum was significantly decreased in T1, T2 group. The breast was also significantly decreased in T1, T2 group. The thigh was not different among treatment, but the cholesterol content of serum was significantly lower in T1, T2 treatment than control. TBARS of the brest and the thigh showed significantly lower than control. POV of the brest and the thigh showed different among treatment, but there were no correlations among treatment.

• Textile Coloration and Finishing
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• v.26 no.1
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• pp.45-52
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• 2014

Red pine (Pinus densiflora) is widely used traditional medicine, pharmacological and nutritional values from which the phytochemical compounds are derived. The present study was aimed to examine the antibacterial effects in the absence and presence of a immature red pine cone extract against 13 microorganisms. The components in the aqueous extract from immature red pine cone were identified by GC-MS. About 1.4% of total polyphenolic compound was measured in aqueous extract collected from immature red pine cone. Also, the high concentration of ${\beta}$-phellenandrene, ${\alpha}$-pinene, limonene, bornyl acetate and aldehyde was detected in total ion chromatograms. Of total 13 microorganisms, 4 microorganisms including Pseudomonas aeruginosa, Vibrio cholera, Listeria monocytogenes, Klebsiella pneumonia were effectively killed by aqueous extract of immature red pine cone. The highest anti-bacterial effect was detected in P. aeruginosa, followed by V. cholera, L. monocytogenes and K. pneumonia. In case of P. aeruginosa, the largest diameter of inhibition zone was maintained to 1/2 solution treated cells and slightly decreased at 1/4 and 1/8 solution treated cells. Also, in test used V. cholera and L. monocytogenes, the inhibition zone was strongly formed in only 1 and 1/2 solution treated cells, while K. pneumonia showed the very small diameter of inhibition zone in all concentrations. Therefore, these results suggested that the aqueous extracts of immature red pine cone should be considered as a new and potentially important anti-bacterial substrate to effectively prevent the microbial infection and penetration.

• Journal of Biosystems Engineering
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• v.19 no.1
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• pp.17-21
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• 1994

This reasearch was performed to provide the fundamental intonation for the mechanization of Korean pine cone harvest when the shoot shearing method is adopted. Shear force and stress of pine cone shoots were measured and analyzed for this purpose. Samples are selected along their harvesting time and tested in 17 levels of shoot diameter from 10 to 26mm with 1mm increment. 1) Shear force-deformation characteristics showed that shoot reached its rupture point after 2 to 4 of bio-yield points. It was supposed that these multiple bio-yield points were caused by the discrete compression of wood parts which are composed of water, nutrient, resin, etc. 2) Required shear force to shear shoot was proportional to the square of shoot diamter, however, shear force for shoots of early harvesting time(Aug. 31) was proportional to the shoot diameter. Variance of shear force was increased as the harvesting time was delayed. Shear forces were distributed from 468N(Aug. 31, 12mm dia) to 4153N(Aug. 31, 26mm dia) disregarding the sampling date. 3) The average shear stresses by sampling dates were 744,822, and 883N/m2, respectively, and for the earlier shoot samples shear stress was quite smaller than the others. Shear stress was proportional to shoot diameter squared, and the effect of shoot diameter on the shear stress was decreased as harvesting time was delayed.

• Journal of Animal Science and Technology
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• v.50 no.1
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• pp.57-68
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• 2008

This study was conducted to evaluate effects of dietary pine cone meal on growth performance, serum cholesterol, carcass quality and fatty acid composition and cholesterol content of meat in broiler chickens. Three treatments were assigned to 480(2 days) Arbor Acre broiler chicks with eight replications, allocating 20 broiler chicks in each replication. Dietary treatments included 1) CS(1% cottonwood sawdust), 2) PCM0.5(0.5% cottonwood sawdust + 0.5% pine cone meal) and 3) PCM1.0(1% pine cone meal). During the overall period the difference of growth performance was not significant among treatments (P>0.05). Also, cholesterol profile(total, HDL, LDL cholesterol and triglyceride) in serum and carcass characteristic were not affected by treatments(P>0.05). TBARS of leg and breast meat was significantly decreased (P<0.05) in PCM1.0 treatment compared to CS treatment. In fatty acid contents of leg meat, C20:0 was significantly higher in PCM0.5 treatment than PCM1.0 treatment(P<0.05). Also, C18:3n3 was significantly lower in PCM1.0 treatment than other treatments(P<0.05). Cholesterol contents of leg, breast meat and abdomen fat were not significant among treatment(P>0.05). In conclusion, results of the experiment were affected by dietary supplementation of pine cone meal on TBARS of leg and breast meat.

• Korean Journal of Poultry Science
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• v.34 no.3
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• pp.223-229
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• 2007

This study was conducted to evaluate effects of dietary pine cone meal on egg production, egg quality, serum cholesterol and cholesterol content and fatty acid composition of egg yolk in laying hens. The total of 252 (51-wks) Hy-line brown commercial hens were used for 5 weeks. Dietary treatments included 1) T1 (basal diet + 1% cottonwood sawdust), 2) T2 (basal diet + 0.5% cottonwood sawdust + 0.5% pine cone meal) and 3) T3 (basal diet + 1% pine cone meal). During the overall period, there was no significant difference in egg production, egg weight and egg quality among the treatments. Lipids profile(total, HDL, LDL cholesterol and triglyceride) in serum was not affected by treatments. Egg yolk cholesterol was not significantly different among the treatments. In fatty acid contents of yolk, C18:3 was higher in T1 treatment than T3 treatment(P<0.05). Total SFA, PUFA, MUFA and UFA/SFA were not significantly different in experimental period. In conclusion, dietary supplementation of pine cone meal affected C18:3 fatty acid content of yolk in laying hens, without any adverse effect on egg production.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.106-106
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• 2022

The cone of Red Pine (Pinus densiflora), which has been used as a drug in traditional medicine. Its ethyl acetate fraction was reported to exert antioxidant, anti-melanogenesis, and anti-inflammation activites. Apoptosis of hepatic stellate cells (LX-2) is regarding as a potential strategy for alleviation of hepatic fibrosis. We conducted to investigated whether the treatment of cone has a potential to control of some factors related in apoptosis and autophagy in cell signaling pathways. We suggest that the cone induced apoptosis through confirming the expression levels of genes (cPARP, Bcl-XL, Bax, p53, and caspase-3) in LX-2 cells. Also, the cone may regulate autophagy (LC3, p62, Beclin-1, and ATG12). Remarkably, the treatment of cone may affect to formation of autophagosomes in the immunofluorescence image in live cells. These findings suggest that the ethyl acetate fraction from the cone of Red Pine (P. densiflora) may have potential as an alternative therapeutic agent for the alleviation and prevention of liver fibrosis.

• Korean Journal of Agricultural and Forest Meteorology
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• v.4 no.3
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• pp.141-150
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• 2002

This study was conducted to reveal the effects of local climatic conditions on the yearly cone production in progeny test stand of Korean white pine. For this, yearly cone production by locality of progeny test stands was first measured and analyzed. The effects of climatic conditions on the cone production was analyzed by the estimation of yearly local climates based on both a topoclimatological method and a spatial statistical technique. From yearly climatic estimates, 19 climatic indices affecting cone production were computed for each of the progeny test stand. The yearly cone productions were then correlated with and regressed to the climatic indices to examine effects of local climatic conditions on the reproductive growth. According to correlation analysis, it was found that some typical climatic indices by locality were significantly correlated with the cone production. Also, the optimal regression equations which can estimate cone production by local climatic conditions were provided for applying to each of the progeny test stand of Korean white pine.

• Korean Journal of Ecology and Environment
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• v.33 no.3 s.91
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• pp.304-310
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• 2000

Enclosure experiments to reduce the growth of Cyanophyceae were carried out using plants in Kyongan stream. Wet plants put into the enclosure at a rate of 2.5 g wet wt/l and at that time, the average concentration of chlorophyll a was ranged from 30 to $50\;{\mu}g/l$. The dominant species was Microcystis aeruginosa. Ginkgo, big cone pine and pine needles significantly inhibited the growth of Microcystis from the early days to the stages of log-Phase. Waterchestnut was the most inhibitory to the growth of Microcystis. Pine needles inhibited in 85% of the algal growth: ginkgo in 80%; big cone pine in 75%; waterchestnut in 78%; wildrice in 59%; and iris in 30%. At the treatment with 0.25 g dried plants/l, algae was declined at a rate of 90% by waterchestnut: 53% by pine needles. Phenolic compounds were purifled from decomposing big cone pine and waterchestnut.

• Journal of Biosystems Engineering
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• v.19 no.1
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• pp.9-16
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• 1994

135 and 136 pine cones were sampled from age class of II to VI Korean pine trees for the study of their physical properties in 1991 and 1992, respectively. The length, width, weight, volume, and the largest projected area of cones were measured, and the specific gravity, apparent volume ratio sphericity, and roundness were calculated. Regression analysis were performed for the weight, volume, and projected area to the cone length and width. The length, and major and minor diameters of the cone stalks were measured and analyzed. 1. The range of the length of cone stalks was 0 to 47.3mm. The average length of stalks were 9mm ('91) and 10mm('92), respectively. Cross section of the stalks was ellipse with average major and minor diameters of 9.1mm and 10.1mm, and 8.6 and 8.7mm in 91 and 92, respectively. 2. The length of pine cones distributed from 8cm to 17cm and the average length were about 13cm('91, '92). The width varied from 5cm to 9.5cm and the average width were 6.7cm('91) and 6.9cm('92). The ratios of the length to the width were 0.56('91) and 0.65('91) and the shape of the cones were found to be ellipse with minor diameter of 1/2 to 2/3 of the major diameter. 3. The roundnesses and sphericity of cones were 0.74 and 0.75('91), 0.63 and 0.67('92), respectively. The average of the largest projected area of cones were $85.3cm^2$('91) and $93.1cm^2$('92) and the criterion areas were $71.0cm^2$ and $74.5cm^2$, respectively. 4. Cone weights were from 83g to 467g('91 and '92) and averages were 186g('91) and 220g('92). The average specific gravities were 0.89 and 0.96('91 and '92). The true volumes were $212cm^2$('91) and $230cm^2$('92), and the average bulk volume was $321cm^2$('91, '92). The average apparent volume ratios of cones were 35% ('91) and 28% ('92), respectively. 5. The weight and the volume were proportional to the length of the cone multiplied by the width squred and the largest projected area was proportional to the length multiplied by the width of cones.