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http://dx.doi.org/10.5764/TCF.2014.26.1.45

Anti-bacterial Effects of Aqueous Extract Purified from the Immature Cone of Red Pine (Pinus densiflora)  

Jeong, Kyung Hui (Department of Biomaterials Science, Pusan National University)
Hwang, In Sik (Department of Biomaterials Science, Pusan National University)
Kim, Ji Eun (Department of Biomaterials Science, Pusan National University)
Lee, Young Ju (Department of Biomaterials Science, Pusan National University)
Kwak, Moon Hwa (Department of Biomaterials Science, Pusan National University)
Lee, Young Hee (Department of Organic Material Science and Engineering, Pusan National University)
Lee, Jae Ho (Department of Biomaterials Science, Pusan National University)
Hwang, Dae Youn (Department of Biomaterials Science, Pusan National University)
Jung, Young Jin (Department of Biomaterials Science, Pusan National University)
Publication Information
Textile Coloration and Finishing / v.26, no.1, 2014 , pp. 45-52 More about this Journal
Abstract
Red pine (Pinus densiflora) is widely used traditional medicine, pharmacological and nutritional values from which the phytochemical compounds are derived. The present study was aimed to examine the antibacterial effects in the absence and presence of a immature red pine cone extract against 13 microorganisms. The components in the aqueous extract from immature red pine cone were identified by GC-MS. About 1.4% of total polyphenolic compound was measured in aqueous extract collected from immature red pine cone. Also, the high concentration of ${\beta}$-phellenandrene, ${\alpha}$-pinene, limonene, bornyl acetate and aldehyde was detected in total ion chromatograms. Of total 13 microorganisms, 4 microorganisms including Pseudomonas aeruginosa, Vibrio cholera, Listeria monocytogenes, Klebsiella pneumonia were effectively killed by aqueous extract of immature red pine cone. The highest anti-bacterial effect was detected in P. aeruginosa, followed by V. cholera, L. monocytogenes and K. pneumonia. In case of P. aeruginosa, the largest diameter of inhibition zone was maintained to 1/2 solution treated cells and slightly decreased at 1/4 and 1/8 solution treated cells. Also, in test used V. cholera and L. monocytogenes, the inhibition zone was strongly formed in only 1 and 1/2 solution treated cells, while K. pneumonia showed the very small diameter of inhibition zone in all concentrations. Therefore, these results suggested that the aqueous extracts of immature red pine cone should be considered as a new and potentially important anti-bacterial substrate to effectively prevent the microbial infection and penetration.
Keywords
polyphenolic compound; anti-bacterial effect; paper disc method; Pseudomonas aeruginosa; Pinus densiflora;
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