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Anti-bacterial Effects of Aqueous Extract Purified from the Immature Cone of Red Pine (Pinus densiflora)

미성숙 솔방울 열수추출물의 항균성

  • Jeong, Kyung Hui (Department of Biomaterials Science, Pusan National University) ;
  • Hwang, In Sik (Department of Biomaterials Science, Pusan National University) ;
  • Kim, Ji Eun (Department of Biomaterials Science, Pusan National University) ;
  • Lee, Young Ju (Department of Biomaterials Science, Pusan National University) ;
  • Kwak, Moon Hwa (Department of Biomaterials Science, Pusan National University) ;
  • Lee, Young Hee (Department of Organic Material Science and Engineering, Pusan National University) ;
  • Lee, Jae Ho (Department of Biomaterials Science, Pusan National University) ;
  • Hwang, Dae Youn (Department of Biomaterials Science, Pusan National University) ;
  • Jung, Young Jin (Department of Biomaterials Science, Pusan National University)
  • 정경희 (부산대학교 바이오소재과학과) ;
  • 황인식 (부산대학교 바이오소재과학과) ;
  • 김지은 (부산대학교 바이오소재과학과) ;
  • 이영주 (부산대학교 바이오소재과학과) ;
  • 곽문화 (부산대학교 바이오소재과학과) ;
  • 이영희 (부산대학교 유기소재시스템공학과) ;
  • 이재호 (부산대학교 바이오소재과학과) ;
  • 황대연 (부산대학교 바이오소재과학과) ;
  • 정영진 (부산대학교 바이오소재과학과)
  • Received : 2014.01.02
  • Accepted : 2014.02.14
  • Published : 2014.03.27

Abstract

Red pine (Pinus densiflora) is widely used traditional medicine, pharmacological and nutritional values from which the phytochemical compounds are derived. The present study was aimed to examine the antibacterial effects in the absence and presence of a immature red pine cone extract against 13 microorganisms. The components in the aqueous extract from immature red pine cone were identified by GC-MS. About 1.4% of total polyphenolic compound was measured in aqueous extract collected from immature red pine cone. Also, the high concentration of ${\beta}$-phellenandrene, ${\alpha}$-pinene, limonene, bornyl acetate and aldehyde was detected in total ion chromatograms. Of total 13 microorganisms, 4 microorganisms including Pseudomonas aeruginosa, Vibrio cholera, Listeria monocytogenes, Klebsiella pneumonia were effectively killed by aqueous extract of immature red pine cone. The highest anti-bacterial effect was detected in P. aeruginosa, followed by V. cholera, L. monocytogenes and K. pneumonia. In case of P. aeruginosa, the largest diameter of inhibition zone was maintained to 1/2 solution treated cells and slightly decreased at 1/4 and 1/8 solution treated cells. Also, in test used V. cholera and L. monocytogenes, the inhibition zone was strongly formed in only 1 and 1/2 solution treated cells, while K. pneumonia showed the very small diameter of inhibition zone in all concentrations. Therefore, these results suggested that the aqueous extracts of immature red pine cone should be considered as a new and potentially important anti-bacterial substrate to effectively prevent the microbial infection and penetration.

Keywords

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