• Archives of Pharmacal Research
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• v.24 no.6
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• pp.527-531
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• 2001

Two triterpenoids, 24-methylene-3,4-seco-cycloart-4(28)-en-3-oic acid (1) and 3-oxo-$9{\beta}$-lanosta-7,22Z,24-trien-26,23-olive (6) were isolated from Abies koreana, together with $\beta$-sitosterol (2), maltol (3), ${\beta}-sitosterol-O-{$\beta}-D-glucoside$ (4), and hexacosylferulate (5). The structures of the compounds were established based on the spectroscopic data. The cytotoxic activities of triterpenoids have been evaluated using the sulforhodamine B (SRB) method. Compound 1 showed moderate cytotoxicities against human lung carcinoma (A549), ovarian carcinoma (SK-OV-3), malignant melanoma (SK-MEL-2), and colon carcinoma (HCT-15) cell lines.

• Korean Journal of Pharmacognosy
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• v.52 no.2
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• pp.105-111
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• 2021

To investigate the antioxidant activities of Pinus densiflora needle (Pinaceae), which is widely used as a traditional medicine in Korea, Caenorhabditis elegans model system was used. The n-butanol fraction of P. densiflora needle methanol extract showed the most potent DPPH radical scavenging and superoxide quenching activities in a dose-dependent manner. The n-butanol fraction was measured for the activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans with reactive oxygen species (ROS) level. In addition, to see that the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the butanol extract, SOD-3 expression was measured using a transgenic strain. As a result, the P. densiflora needle n-butanol fraction increased SOD and catalase activities, and decreased ROS accumulation, dose-dependently. Furthermore, the n-butanol fraction-treated CF1553 worm showed higher SOD-3::GFP intensity than the control.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.108-108
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• 2022

Pine (Pinaceae family such as Pinus densiflora, P. rigida, and P.taeda) has been used as traditional medicine, its various parts (pine needle, bark, sap) have been used for hemostasis, bruises, and burns. These species were reported that have phenolics and flavonoids. We evaluated the anti-inflammation effects of PRT in lipopolysaccharide (LPS)-induced macrophages. These results showed that the ethyl acetate fraction of cone from Pinus rigida x P.taeda (ECRT) stabilized free radicals by reducing reactive oxygen species (ROS) and decreasing the production of nitric oxide (NO). ECRT decreased the expressions of nitric oxide synthase (iNOS) and cyclooxygenase (COX-2). In addition, ECRT significantly suppressed mRNA levels of inflammation-related factors such as cytokines, iNOS, and COX-2. As a result, ECRT was related to alleviating various pro-inflammatory mediators through IκB/NF-κB signaling pathways, including p65 translocation to the nucleus.

• Journal of Korean Society of Forest Science
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• v.98 no.4
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• pp.464-471
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• 2009

Vegetation types of the native conifer forests in the ridge of the Baekdudaegan, South Korea, were classified using the Braun-Blanquet method and TWINSPAN and its ecological characteristics analyzed with special repect to floristic composition. The vegetation type was classified into two types (Pinus densiflora-Carex humilis and Pinus koraiensis-Dryopteris crassirhizoma types) and seven groups. Mean species richness and total plot cover per unit area ($/100m^2$) were $21.9{\pm}8.9$ spp. and $146.9{\pm}32.3$%, respectively. The plant species composed of 82 families, 217 genera, and 387 species and the three major families, Compositae, Liliaceae, and Rosaceae, made up about 25.6% of the total species. The 7.8% of total families were the families with occurrence of only one species. The highest relative importance value was 19.2, the species belonged to Pinaceae and the pattern was different from species richness pattern of other families.

• Journal of Korean Society of Forest Science
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• v.98 no.4
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• pp.472-478
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• 2009

Pine (Pinus densiflora) forests of 58 sites on the ridge of the Baekdudaegan, South Korea were classified by applying the phytosociological method and TWINSPAN. The floristic composition and life-form characteristics of the vegetation types analyzed and documented. The vegetation types were classified into five groups including four groups of Carex humilis var nana type and one group of Abies koreana-Sasa borealis type. Compositae was the most diverse family (26 species), Liliaceae (21 species), Rosaceae (18 species) and Betulaceae (11 species) whereas 27 families were represented by only one species. Taxonomically, Compositae was the most diverse (17 genera), Liliaceae and Rosaceae(12 genera) whereas 41 families were represented by only one genus. The Fagaceae was the most important family in total coverage (19.0) and followed Pinaceae (16.7) and Ericaceae (15.1). Five families such as Compositae, Liliaceae, Rosaceae, Betulaceae, and Umbelliferae contributed little to total coverage, although represented by numerous species. In the correlation between species richness and life forms, the species richness was highly correlated with growth and disseminule forms. In general, the correlation coefficients between life forms represented lower values than those between species richness and life-forms.

• Applied Biological Chemistry
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• v.41 no.1
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• pp.95-98
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• 1998

Methanol extracts of 54 plant species in 32 families were tested for their antignawing activities against mice by wire-dipping method. The activity varied with plant species. Potent antignawing activity was observed in extracts from roots of Aucklandia lappa (Compositae), barks of Cinnamomum cassia (Lauraceae), fruits of Illicium verum (Magnoliaeceae), fruits of Piper nigrum (Piperaceae), rhizomes of Rheum officinale (Polygonaceae), and leaves of Pinus densiflora (Pinaceae). As naturally occurring rodent repellents, these plant-derived materials could be useful as a preventive agent against various kinds of damage caused by rodents.

• Korean Journal of Environment and Ecology
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• v.18 no.4
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• pp.415-436
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• 2004

The vascular plants species were summarized as 479 taxa: 89 families, 260 gents, 404 species, 66 varieties, 8 forms and 1 hybrid in the whole 6 surveyed areas of eastern parts of Jirisan (Georim Valley, Baengmudong Valley, Hanshin Valley, Jungsanri Valley, Sesokpyongjeon, and Chonwangbong). Based on the list of Korean endemic plants, 26 species were recorded: Athyrium excelsius (Aspidiaceae), Abies koreana (Pinaceae), Betula ermani var. saitoana (Betulaceae) etc. The special plants from the list of Ministry of Forest, the Forest Research Institute (1997) and the Ministry of Environment (2001), 15 species were recorded: Smilacina bicolor (Liliaceae), Disporum ovale (Liliaceae), Clematis chiisanensis (Ranunculaceae) etc. And 15 species of naturalized plants were recorded: Oenothera biennis (Onagraceae), Ambrosia artemisiaefolia (Asteraceae), Carduus crispus (Asteraceae), etc. Especially, the Korean endemic plants repeatedly appeared around mountain-trails and Ambrosia artemisiaefolia (Asteraceae), also, popular were distributed in the entire studied areas.

• Journal of the Korean Wood Science and Technology
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• v.42 no.5
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• pp.533-542
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• 2014

We extracted essential oils from four species (Pinus densiflora, Larix kaempferi, Pinus koraiensis, and Abies holophylla) in the family Pinaceae to investigate their antibacterial activities against respiratory tract bacteria (Klebsiella pneumoniae, Haemophillus influenzae, Streptococcus pyogenes, Streptococcus pneumonia, and Neisseria meningitides). Among the tested oils, that from A. holophylla was showed strong activity based on disc diffusion and broth medium dilution (minimum inhibitory concentration, MIC) assays. Qualitative analysis of A. holophylla oil was carried out by GC-MS; ${\alpha}$-pinene, camphene, ${\beta}$-pinene, 3-carene, limonene, bornyl acetate, borneol, ${\beta}$-caryophyllene, ${\alpha}$-caryophyllene, caryophyllene oxide, and ${\alpha}$-bisabolol were identified as its major constituents. Fractionation by silica gel chromatography was performed to analyze the active constituents of the crude oil. In particular, one fraction containing caryophyllene oxide as the major constituent showed stronger antibacterial activity than the crude oil of A. holophylla. Growth rates of bacterial strains exposed to fraction D were explored by optical density (OD600) measurements while morphology was examined by optical microscopy observations ( ${\times}1000$). OD600 of K. pneumoniae decreased from 0.2582 to 0.005 in response to treatment with fraction D at a MIC value of $0.31{\mu}{\ell}/m{\ell}$.

• Journal of the Korean earth science society
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• v.26 no.3
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• pp.276-282
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• 2005

The Quaternary palynoflora from Cheongsu core, drilled on the western part of the Jeju Island, consists mainly of deciduous broad-leaved angiosperms and herbs. Dominant taxa are Pinaceae, Alnus, Castanea/Castanopsis, Ulmus/Zelkova, Chenopodiaceae and Compositae. Based on palynofloral composition, two palynostratigraphic zones are recognized. Zone I is characterized by a predominance of Alnus and Chenopodiaceae, and by the abundance of herbs. Zone II is dominated by conifers and deciduous broad-leaved angiosperms, including Abies, Taxodiaceae-Cupressaceae-Taxaceae, Ulmus/Zelkova and Gramineae. The Cheongsu palynoflora can be correlated with Alnipollenites(Alnus)-Chenopodipollis(Chenopodiaceae)-Compositae Assemblage Zone of the East China Sea and is assigned to the Pleistocene in age. Ecology of main taxa and comparison with the present vegetation of Korea suggest a cool temperate climate and a low relief in the Jeju Island during the deposition of the Cheongsu core sediments.

• Journal of Species Research
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• v.4 no.2
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• pp.159-168
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• 2015

Although airborne pollen is invisible to the eye, it has been known as a major source to respiratory allergic reactions. For this reason, airborne pollen is monitoring in many countries to predict pollen concentration based on locality and season. However, the morphological characteristics of airborne pollen and their potential tendency as an allergen are still obscure. In the present study, we selected 52 airborne pollen samples based on previously reported data and investigated their detail pollen characteristics using LM and SEM. Major airborne pollen in Korea has sorted in 19 families (most angiosperms except four gymnosperm families), and all pollen grains are small to medium in size ($P=17.34-49.86{\mu}m$) apart from the bisaccate pollen grains of Pinaceae ($P=46.49-106.20{\mu}m$). The aperture number and shape vary from sulcate to polyporate. While the inaperture pollen has found only in gymnosperm (Cupressaceae and Taxaceae), triporate or polyporate is common pollen type in angiosperm. The sexine ornamentations could divide into several types, but the most sculpturing types are inconspicuous like psilate, rugulate and granulate. Reticulate pollen grains as a semitectum have occurred the species of genera Platanus and Fraxinus only. To estimate the possible relationships between pollen features and allergen, the results are discussed in botanical context.